LOCUS       Exported                 822 bp ds-DNA     linear   UNA 24-SEP-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Dec 11, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     promoter        15..33
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             53..769
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Miesenbock et al., 1998)"
                     /label=ecliptic pHluorin
                     /note="responds to a drop in pH with a gradual loss 
                     (eclipsing) of excitation at 475 nm, accompanied by a 
                     reduction of excitation at 395 nm"
                     /note="color: #05fd14"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFHTSTLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             242..244
                     /codon_start=1
                     /label=L64F
                     /note="color: #993366"
                     /translation="F"
     CDS             245..247
                     /codon_start=1
                     /label=T65S
                     /note="color: #993366"
                     /translation="S"
     CDS             539..541
                     /codon_start=1
                     /label=V163A
                     /note="color: #993366"
                     /translation="A"
     CDS             659..661
                     /codon_start=1
                     /label=T203H - PA mutation
                     /note="color: #993366"
                     /translation="H"
     CDS             731..733
                     /codon_start=1
                     /label=227A
                     /note="color: #339966"
                     /translation="A"
ORIGIN
        1 tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta gcatgagtaa
       61 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
      121 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
      181 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac
      241 tttctcttat ggtgttcaat gcttttcaag atacccagat catatgaaac ggcatgactt
      301 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga
      361 cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat
      421 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata
      481 caactataac gatcaccagg tgtacatcat ggcagacaaa caaaagaatg gaatcaaagc
      541 taacttcaaa attagacaca acattgaaga tggaggcgtt caactagcag accattatca
      601 acaaaatact ccaattggcg atgggcccgt ccttttacca gacaaccatt acctgtttca
      661 cacttctact ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt
      721 tgtaacagct gccgggatta cacatggcat ggatgaacta tacaaataat ctagaactcg
      781 agtctagagg gcccgtttaa acccgctgat cagcctcgac tg
//
