LOCUS       pAsCpf1-AIO(U6_with_As 10092 bp ds-DNA     circular     29-DEC-2017
DEFINITION  .
FEATURES             Location/Qualifiers
     primer_bind     10055..10080
                     /label="SP6"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     CDS             8222..8881
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      8979..9661
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    complement(2632..6552)
                     /label="humanized AsCpf1"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(2578..2583)
                     /label="GS linker"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    complement(2584..2631)
                     /label="Nucloplasmin NLS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(6559..7543)
                     /label="CAM 5' UTR"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1187..1691
                     /label="Pf U6 3' regulatory element"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    9..1158
                     /label="Pf U6 5' regulatory element"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_binding    10001..10023
                     /label="LacO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(2554..2577)
                     /label="FLAG"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1159..1178
                     /label="AsCpf1 5' DR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2545..2550
                     /label="Pb DT 3' UTR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    complement(1700..2544)
                     /label="Pb DT 3' UTR"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer_bind     10029..10049
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
ORIGIN
        1 gcggccgcAA CGGTAAAAAT AATAACACGA ATTATCACAA TAATTTTTCA CATATGTATA
       61 TATATATATA TATTATCAAA GGGACAACCG TTTCAAGAAA GCAAATGTTA TTATTTTGTT
      121 TTTATTCTAA TGTATGTAAG CCAAAAACCA ATAAAAAAGT AATAATTAAA AAGGAAATAA
      181 AATAAATATA CATATGTAAT TATACTTATA TAATAAGTAT GTATATTTTT TATTATTTTT
      241 TTTTTTTAAT GCTATTTATT TATTATTGCA TCTTTTTATT TAATTTTTGT ATTTTTTTAT
      301 ATAATTCCTT TATTTCTTTA TTTCTTTCTT TATTTCTTTT TTTTTTTTTT TTTTTTTTTT
      361 TTTTTCCTTT AATTAAACTT TAAAAGACGT TATAAATTTT AAACATATTT TATAAATAAA
      421 AAAAAAAAAA AAAAAGACGA AATAATAAAA TAAACGAAGA TAAAGTAATA TTTTGTTATT
      481 ATGTCACATA GTATGTGTAC TACAATTATT TATGGATGCA AATTATTAAA TTGGTAGTTA
      541 TTATATACAT ACATGTTTTT TTTTTTGTGT GTATTAAAAG GAACTATTAT ATAAATAGAA
      601 CAATTTCATG ACATTTGGAT TTCTACACAT CTTGAGGTTT TACAATAATA TCTTCTTTTT
      661 TTTTTTTTCT CTTCTATATA TGAAATATAT TGTAAAAAGG TACAATATAT ATAATATTTA
      721 TATACTATGA AAAAAAAAAA ATATATACAT AAACTAATAA TAATTTATTC TAATTTAATA
      781 TAAAAAAAAA TTCTTGCTTG TTCAGATAAA TAAAAGAAAT TTATATAAAT AGCTATATTT
      841 TTTTTTTTTT TTTTTTTTTT TTTTTCTAAT TATAAATATA ATTATTCATT TAAAAAATTT
      901 TAAATATTTA AATATTTCAT AAATTAAAAA AAAAAAAAAA AAAACTATTA ATATAATAAA
      961 CTTTTATTTT TACTGTAATA TAATTTTTTA TAATGTAAAA ATAAAGGGTA AATTATTATT
     1021 AAAAAATGTA TATGTTATGT ATATATAACA TAATATATTA TAATATATAT ATATATATAT
     1081 ATATATATAT AATATTAGAG TAACCAAAAT GCATAATTTT TCCTATATGC ACATATTTCA
     1141 TATTAAGTAT ATAATATTTA ATTTCTACTC TTGTAGATct taagTTTTTT ATTATTTCCT
     1201 ATAAAATAAT ATATATATAT ATATATATAT ATATATATAT ATATTTTAAA TATAATGAAG
     1261 TTTTTAAGGC TACCATAAAA AAAAAAATAC ATATATATAT AAATAAATAT ATTATATTAT
     1321 ATAATATATA TATTAATGTT TTTGTTTTTT AATATTTTTT TTTTTTTTTG TAAAATATAT
     1381 TATTTTTGGA CTTATAAATA ATTGTGTACA TACATATATT ATTTTATTAT AATAAATATA
     1441 ATAATACTAT AATAATCTTT ATGCAATATT TGTAATTCAT ATATAATAAT TTTTAAAATT
     1501 ATCTATCTTT TGAAAAAAAA AAAAAAATAA AAATAATAAA AATTATAATG TTATTAAATA
     1561 CTATAATTAT TATGATTAAT ATAATATATA TATATATATA TATATATATA TATATATATA
     1621 TATATTTTAT ATCATTTAAT ATATTATGTG TGATATAATA ATAAATCCAT TGTCCATAAA
     1681 TAGATGAAAT Agcggccgcc taccctgaag aagaaaagtc cgatgatgtt gtggacataa
     1741 agaattgagc tgtctcaaaa aatgagaaaa caaacaaaca aacaaaatac cattatgcgc
     1801 atatcctctg ctcaatatca atttttcttt atgccagttt tgtttcgaca ctatttgagc
     1861 aacaccttcc ctttactatc tactactgtt acaatgttat tttattcacc tttttacaat
     1921 tttatcaaaa tttttttgta tgcatataaa ttatacattg taaagccaaa gtgtagataa
     1981 agacaccttg tttatttttc cccaattttt tttttttacc atttttttat taaccttgct
     2041 catttacctg ctaatacgat tgctatatga tatcgaaatt gaaggaaaaa ccatcatttg
     2101 tgtttatatg ttttttttta atttttcaac tttttttatt taactatgct aatcatatta
     2161 taaacatttt aaaaatcaag tccaactatt tatgaatcat tgaaggagac acataattat
     2221 aacagtaaaa ataataataa taataataat tttgtcttat tgtttcaaac atattatgtt
     2281 ccataaatat ctaaattttt aatatggtca taaattcata ggatttaatt tatataaaat
     2341 atggctattc atactagcca ttttatgtgt gtaaagtttt ttttttctga acaaaaaata
     2401 aaatgttcat aattttagct atttacatgc atgtgcatgc acaaaaaaaa atatgcacac
     2461 aacatacaca tttttacagt tataaataca atcaattggt ataaatatat aaataagaaa
     2521 aacgaacatt aagctgccat atccctcgag ttacttgtcg tcgtcgtcct tgtagtcGGA
     2581 TCCCTTTTTC TTTTTTGCCT GGCCGGCCTT TTTCGTGGCC GCCGGCCTTT TGTTGCGCAG
     2641 CTCCTGGATG TAGGCCAGCC AGTCCTGATT GGAGATGCCG TTCTGCAGCT TCAGATCCTT
     2701 GCTCTCCTTC AGGTGATTCA GCAGCAGCTG GCCCTTCAGG GCGATGTGGT AGGCGCCATT
     2761 GGCATCGGCG TCCATGGGCC ACTCTGGGTT CTGAAACCGG GAGTCGAAGC ACACGCCATT
     2821 CAGATCGCGC ACGGGGCTGT TGATATAGTC CTCGCCTGTG GCGGCATTGG AGTTCCGCAT
     2881 CTGCAGCACG CTGCGGATCA GGGCCACCAT GGTGTCGATG GCGTGAGAAT CGTCATTCTC
     2941 CAGCAGCTTT GGCAGGATGT TGGAGCCATC CCTGAACACG ATGCCCTTCT CCTCCAGCAG
     3001 GGCGATCAGC TCGTTGGCAG GATACAGGTC CCGGTATCTG CCGGTGAATC TGTGATTCTC
     3061 GATCACTGGC ACGATTCTCT TGCCGGCGAT GAAAGGGGTG CCCTTGGCGT CAAACTGTGT
     3121 CTCGTTCTTC TCGAACACGA TATCCCATGC AGGCATAAAG CCGGGCAGGC CCCTCTGGAA
     3181 GGACAGATTT CTGTTCATCT TAAAGTGCAG GATGAAGTCG CCGGTTTTCA CGTCGTAGTG
     3241 CAGAAAGTCG AAGCCCTCCA GGAAGTGCTT GCGGCTCTCG TGATTCTTGA TGGTTTTCCA
     3301 CACGAAGGGG TCCACGAAGC CGGTCAGGGG ATCGATCTTA GATGTATATG GGGCAGGCAC
     3361 GTAAAACAGG AAGCCAGACT GGGTGCCCAT CTTGGCAAAG GAGGTGAACT GGTCTGTCAG
     3421 CTGGTATGGG TTCAGCACGC CTCCCACTTT CTCTGCTGGA TAGTCCTTCA GCACCAGGCA
     3481 ATTCAGCTTA TCGATCAGCA TCTTCTCGAA CTGCTGGTAC ACGGCCTTCT CGGCGATGCC
     3541 GGTCCTCTTG CTCTTAAAGC CGAAATTCAG GTTCTCCAGC ACCACCACGG CCTGGTAGTG
     3601 GATCATCAGG TCCACGATCT CGTGGATGAC CTGGCTCAGA TAGCCCTGCT TCAGATCCTT
     3661 GATTGTGCCC ACCACAGACC AGGCCTGCCT TGCTGCCACC CTCTCCTTCT CCCTGTTGTC
     3721 CAGCTTCTTC TGGTAATCAA ACTGCTGGAT GGTGTTCAGG CTCCGCTGCT CCAGGATCTT
     3781 GCCGGTGGAG TCGATCACTG TGATATAGAT CAGGTTTCTC TCGCCCCGAT CGATGCCGAT
     3841 GATAGGTGTC TCGGGGTGCT CCTTCAGGTA GGCATTCACC CTCTGGTTGA ACTTAGATGG
     3901 GGAATTGGCG GCCTGATAGT TCAGTGTGAT AGGCACGTGG AAAAAGAACT TGTCGCTGGT
     3961 AAAGCGCCTA TCCTTGATGA TCTCGTGAGA CACCTCCTTG GTGATCACGT TGGGCAGCAG
     4021 GGCCCTGGCC TCATCAGACA GGTCGTGGGA CAGTCTGTGA TTCACATAGT CGTACAGCTC
     4081 CTGGTACAGG GTGTCGGGGA TTGGGGTTTT CTGATCCTTC AGCTTCTTGT TCAGCATCTT
     4141 CTCTCCCAGC CGGTGTGCCA TCCTCTTCAT CCTGGACTTA GGGCGGTAGA ACAGCTCGGC
     4201 CTGGCCATTC AGCTTGATGC TTGTCTTGGC CAGGTTCTCT GGAGAAAACA GGCCGGTCCA
     4261 ATACAGTGTG TGCAGATTAG GCTTGCCGTG GTGGCCCTTG GCAAAGTCCT TGTTATAGAT
     4321 CTGGAACAGG TACAGCTTGC CTGTCTCCAC GGCATCCATG ATCTCCTTCT CGGCGATTCT
     4381 CTGGAAGCTG ATGTGGTACA GCAGGGGATT CAGCTCGGCA TAGTACTCGC CCAGGTCCTT
     4441 ATACTGAGAG GATGGCCGCA GGCTAGACAG ATCGATAGAG GTTGTCTTGG TATACTTGGA
     4501 CAGAAAATCC CTTGTGAAGT CGATCCACTT GCACAGGGCC TCTCTGTAGC CCTTCTGGTC
     4561 GCCGGTTTTC TTGGCGTAGG CTGTCTGAAA CTTCTTTGGC TCCTTCTCAG GATTGTTCAG
     4621 GTCGTAGATC TCCTTTGTGA TCTCCAGAGG CTCGATGAAA TTGTTGGACA GCAGGATGGG
     4681 GGTTGTGTGG GTCTGAAAGT GGGCTGTCAC GGCCTTCAGC TGGGTGCTGC ACTTTGGGAT
     4741 CATCTTGGCG GCATCAGGGA AGTAGTCATA GTACATCTTA TCAAAGCCCT CGCTGGTTTT
     4801 CTCTGTGGGC TCGAAGCTCA GGGCCTTATA CCTGCCCTTC TGCTTTGGCA TGATGCCCAG
     4861 ATAGTACAGG CCGTTCTTCA CAAACAGGAT GGCGCCATTG TTCTTCTCCT TATTCACGTC
     4921 CCAGCCAGAG GCCAGTGTAG GCATCTGAAA GTTCAGCTTG AACTTCTCCA CGGAGTAGGG
     4981 CTTCTTGGTG GCATAATTTC TGGCCTTGTT GTAGAAGCTC AGAGAAGGCT CCATCTCCAG
     5041 CTTGATGCCG GTCAGCCGGG CAGAGAACTC GGGGTCCACC TCGTTGGACT CATCCACGGC
     5101 AAACCAGTCC AGCAGGTGGT ACAGGCCCAG CAGGCTGTCC AGCTGAGACT TCAGGATCTC
     5161 CTTCTCCTCC TGCTTCTTCA GGGTTGTAGG CAGTGGCTGA TCCAGGGCGG CGTGTGCGTG
     5221 GGACAGGATC TCGCTGGTTT TCTGCTTGAA GGCCTCGCTC AGCTCCTTGC CTGCGGCAGA
     5281 GATGATCTCC TGCAGGTTGA TATCCTCGTG CTTCAGGCTG CGCTGCACCT TCTCCTTGGC
     5341 AGACTTGGTG ATCTTGCCTG TCAGCTCGGA GATTCTCCGC TCATACAGGG CATTCCTCAG
     5401 TGTATCCCAG TGGTCGCACA GGGCGCTGCT GATTGTCTCC AGCTTCTTGT GGCTGATGAA
     5461 GATGTGTGTC AGGTCGATGC TGTTCAGCTC GTTAAACAGG GCCTCGGCTG TCTCCAGCAC
     5521 GTTCTCGTTT CTCAGCAGTG TCTTGTACTT GCAGAAGGAC TGGATCACTT CCTCGTCGCT
     5581 CTTAAACTCC TCCAGGATGA AAGACAGGGT GTTCCTATCG GACAGGATCT GCTTAAACAG
     5641 GGGGATGAAT CTGTGTGGCA GGGAGGCGAT GATGTGGGCT GTCTCATCAT TCTTCTGGAT
     5701 GGCCAGATTC AGCACCTCGT TCAGGCCCTT GATCTTCTCG GTGCCTGCCT CCCGAGAGAT
     5761 TCCTCCCAGC AGCTGGTTAT ACAGGTCGAT CTGGGTCTGT GTCAGCAGCT GGTTATAAAA
     5821 AGGGAAGGAA AACACCTCCT CGATGGAGGT GCTCACGAAG ATGCCGATGG CCTTCTTCAC
     5881 GTTCTCAAAG TGCTCCCGCA GGCTGGGCAC GGCGGTGATC AGGCGTGTGA AGATGTGACA
     5941 ATTCTCCTTA AACTTGGGGA AGTTGTCCTG CACGATGCGG TGTGGGATGG CTGTGCTGAT
     6001 ATCCTCGGCG CTGAACACGT TCTTCCTGTT CTCATAAAAG CCGGAGAAGT AGGTTGTAAA
     6061 CTTGTCGAAG CTCCGCAGCA GGGCGTTCTC GTGCTCGGTT GTGGTCACGG TGCCCAGCTG
     6121 CTTCAGCACC TTGCCATTAA ACAGCTCGGC CTTGAACAGG CCCTTGTAGA TCTCGGCGTG
     6181 TCTCTTATTG ATGGCATCGG TCAGGTTGTC TGTCCGGCCG ATGAAGTAGT CGTGGATGGC
     6241 ATTGCGATAT GTGGCCTGCT CCTCGATCAG GGCGTTCCTT GTCTCCTCGG TTTTCTCCTT
     6301 TCTATAGGAG TCGATGGCGG CGCTCAGGTT CTCCCAATCC AGCTGCACCA GCTGCAGGCA
     6361 CTGGTCGGCA TAGGTCTTGT AGATCCGATC GATGATGGGC TTCAGCTCCT TGTAGTGATC
     6421 ATTGCGGGCC TTGTCCTCCT CGATGAAGCC CTGCTCCTGG ATGTGCTTCA GGGTCTTGCC
     6481 CTGTGGGATC AGCTCAAACC GCAGTGTCTT GCTCACCTGA TACAGGTTGG TAAAGCCCTC
     6541 GAACTGTGTC ATggatccTG ATATATTTCT ATTAGGTATT TATTATTATA AAATATAAAT
     6601 CTTGAATGAT AATAAATAAA ATATTAGTTA TTCCTTTTCT AGTTTAAAAT ATACATATTA
     6661 TAAATATATA TATATATATA TATATTTTTA TTGTGACAAG AATATATAAT TATAAATTAT
     6721 ATTATTTATT TTTGTATTTT TTTTTTTTTT TTTTTTTTTT TCTTTTTTTG TTTTATTTTT
     6781 CTTTTTTTTT ATAAATATTA TTTTTTTCTT TTATCATGCA CATTGGAATA ATACATTAAT
     6841 ATATATATAT ATATTATATT ATACATATAT TGAATAATGT TTATAAAAAA TGCATAACTT
     6901 ATATGAATAT AATTTTTTTT AAATATGACA AAAAGAAAAA AAAAAAAAAC CAAAAAAAAT
     6961 TAAAATTGAA ATGAAATATA TAAATATATT ATTTATATAT ATTATACATT GTTTAATACT
     7021 ACTACATGTA TATATATATA TTATATATAT ATATATATAT CAATTTTTTC AAAAATAAAT
     7081 TAATATAAAA AGAGGGGAAA AAAAAAAAAA AAAAAAAAAA AAGATAATTA AGTAAGCATT
     7141 TAAAAATATA TAAATTGATA ATATATAAAA TTAATCACAT ATAAACTAAT ATAATTTATA
     7201 AAATAAGGAA AATAAAATAT TACCATAAAA TAAAAATAAA AATAAAAAAA AAAAAAAAAA
     7261 ACACCTTTTT TTATATATAT TAATATATAA TTATCTCTTA GAAAAAATAT TGTATAATTA
     7321 TATATGTAAT GATTTATATA AAAAAATAAA ATTATACAAG TATATATTTT GTTTCTATAA
     7381 ATTGATATCT TAATTATTTA TTATTAGAAA TAGATATTTT TATAATAAAC CAATAGATAA
     7441 AATTTGTAGA GAAAAAAAAA TAAAAATAAA AATAAAAATA ATATAATATA TAATAAAATA
     7501 AAATAATATT ATATAAATAT ATTTTAATTT TTTTTACAAA ATGtctagaa ttcatatcga
     7561 taactccatg gaactcctag gctggcgcct gatgcggtat tttctcctta cgcatctgtg
     7621 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     7681 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     7741 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     7801 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     7861 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     7921 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7981 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     8041 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     8101 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     8161 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     8221 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     8281 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     8341 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     8401 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     8461 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     8521 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatgccaac
     8581 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     8641 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     8701 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     8761 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8821 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     8881 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     8941 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     9001 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     9061 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     9121 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     9181 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     9241 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     9301 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     9361 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     9421 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     9481 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     9541 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     9601 tcgatttttg tgatgctcgt caggggggcg gagcctatcg aaaaacgcca gcaacgcggc
     9661 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     9721 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     9781 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     9841 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     9901 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     9961 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
    10021 atttcacaca ggaaacagct atgaccatga ttacgccaag ctatttaggt gacactatag
    10081 aatactcaag ct
//