LOCUS       pCX_mTagBFP2_mT2        7498 bp    DNA        circular     01-DEC-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Dec 1, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
COMMENT     Created: Friday, August 26, 2005 5:19 PM pCAGGS sequence from LMBP
            Martine Vanhoucke ;Nucleotide sequence of pCAGGS Date: 21/02/1992
            (Latest Update: 18/10/1999) ;Start of the nucleotide sequence in
            the middle of the first EcoRI site ;downstream from the AG
            promoter.    ;(c) BCCM/LMBP Plasmid collection (Ghent University,
            Belgium).
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     enhancer        11..388
                     /note="hCMV"
                     /locus_tag="hCMV"
                     /label="hCMV"
                     /ApEinfo_label="hCMV"
                     /ApEinfo_fwdcolor="#5ac3fa"
                     /ApEinfo_revcolor="#5ac3fa"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    670..762
                     /note="Exon 1?"
                     /locus_tag="Exon 1?"
                     /label="Exon 1?"
                     /ApEinfo_label="Exon 1?"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     intron          763..1687
                     /note="intron"
                     /locus_tag="intron"
                     /label="intron"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#eb606c"
                     /ApEinfo_revcolor="#eb606c"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1652..1672
                     /note="HN737"
                     /locus_tag="HN737"
                     /label="HN737"
                     /ApEinfo_label="HN737"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1696..1715
                     /note="HN382"
                     /locus_tag="HN382"
                     /label="HN382"
                     /ApEinfo_label="HN382"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1745..2455
                     /note="mTagBFP2"
                     /locus_tag="mTagBFP2"
                     /label="mTagBFP2"
                     /ApEinfo_label="mTagBFP2"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1943..1951
                     /note="mTagBFP2 chromophore"
                     /locus_tag="mTagBFP2 chromophore"
                     /label="mTagBFP2 chromophore"
                     /ApEinfo_label="mTagBFP2 chromophore"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2456..2530
                     /locus_tag="T2A"
                     /label="T2A"
                     /ApEinfo_label="T2A"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2540..4516
                     /locus_tag="iCreERT2"
                     /label="iCreERT2"
                     /ApEinfo_label="iCreERT2"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             2540..3562
                     /codon_start=1
                     /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLL
                     S
                     VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLP
                     R
                     PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFL
                     G
                     IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVER
                     W
                     ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQR
                     Y
                     LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLED
                     G D"
                     /locus_tag="iCre"
                     /label="iCre"
                     /ApEinfo_label="iCre"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             3575..4510
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human
                     estrogen receptor (Feil et al., 1997)"
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPI
                     L
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLE
                     I
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEE
                     F
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQR
                     L
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEE
                     T DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
                     /locus_tag="ERT2"
                     /label="ERT2"
                     /ApEinfo_label="ERT2"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           4526..4617
                     /note="rabbit b-globin 3' UTR, polyA"
                     /locus_tag="rabbit b-globin 3' UTR, polyA"
                     /label="rabbit b-globin 3' UTR, polyA"
                     /ApEinfo_label="rabbit b-globin 3' UTR, polyA"
                     /ApEinfo_fwdcolor="#f76ca1"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     3'UTR           4618..4974
                     /note="rabbit b-globin 3' flanking region"
                     /locus_tag="rabbit b-globin 3' flanking region"
                     /label="rabbit b-globin 3' flanking region"
                     /ApEinfo_label="rabbit b-globin 3' flanking region"
                     /ApEinfo_fwdcolor="#f76ca1"
                     /ApEinfo_revcolor="#008040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        complement(5052..5134)
                     /note="lac"
                     /locus_tag="lac"
                     /label="lac"
                     /ApEinfo_label="lac"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    5276..5351
                     /note="SV40 origin"
                     /locus_tag="SV40 origin"
                     /label="SV40 origin"
                     /ApEinfo_label="SV40 origin"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    5382..5456
                     /note="SV40 early region polyA"
                     /locus_tag="SV40 early region polyA"
                     /label="SV40 early region polyA"
                     /ApEinfo_label="SV40 early region polyA"
                     /ApEinfo_fwdcolor="#ff3eee"
                     /ApEinfo_revcolor="#ff3eee"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    complement(5382..5456)
                     /note="SV40 late region polyA"
                     /locus_tag="SV40 late region polyA"
                     /label="SV40 late region polyA"
                     /ApEinfo_label="SV40 late region polyA"
                     /ApEinfo_fwdcolor="#ff3eee"
                     /ApEinfo_revcolor="#ff3eee"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(5571..5972)
                     /note="pMB1 origin"
                     /locus_tag="pMB1 origin"
                     /label="pMB1 origin"
                     /ApEinfo_label="pMB1 origin"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(6513..7373)
                     /note="bla"
                     /locus_tag="bla"
                     /label="bla"
                     /ApEinfo_label="bla"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 cacctgggtc gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
       61 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
      121 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
      181 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
      241 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
      301 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
      361 atctacgtat tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact
      421 ctccccatct cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt
      481 tgtgcagcga tgggggcggg gggggggggg gggccccccc caggcggggc ggggcggggc
      541 gaggggcggg gcggggcgag gcggaaaggt gcggcggcag ccaatcagag cggcgcgctc
      601 cgaaagtttc cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg
      661 cggcgggcgg gagtcgttgc gcgctgcctt ccccccgtgc cccgctccgc cgccgcctcg
      721 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
      781 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg
      841 ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg
      901 ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc
      961 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc
     1021 gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg
     1081 cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc
     1141 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
     1201 acggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
     1261 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aaggggcgcg gcggcccccg
     1321 gagcgccggc ggctgtcgag gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg
     1381 cgagagggcg cagggacttc ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg
     1441 ccgccgcacc ccctctagcg ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat
     1501 gggcggggag ggccttcgtg cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg
     1561 gggctgtccg cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt
     1621 ctggcgtgtg accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt
     1681 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt
     1741 cgccatggtg tctaagggcg aagagctgat taaggagaac atgcacatga agctgtacat
     1801 ggagggcacc gtggacaacc atcacttcaa gtgcacatcc gagggcgaag gcaagcccta
     1861 cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt
     1921 cgacatcctg gctactagct tcctctacgg cagcaagacc ttcatcaacc acacccaggg
     1981 catccccgac ttcttcaagc agtccttccc tgagggcttc acatgggaga gagtcaccac
     2041 atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct
     2101 catctacaac gtcaagatca gaggggtgaa cttcacatcc aacggccctg tgatgcagaa
     2161 gaaaacactc ggctgggagg ccttcaccga gacgctgtac cccgctgacg gcggcctgga
     2221 aggcagaaac gacatggccc tgaagctcgt gggcgggagc catctgatcg caaacgccaa
     2281 gaccacatat agatccaaga aacccgctaa gaacctcaag atgcctggcg tctactatgt
     2341 ggactacaga ctggaaagaa tcaaggaggc caacaacgag acctacgtcg agcagcacga
     2401 ggtggcagtg gccagatact gcgacctccc tagcaaactg gggcacaagc ttaatggcgg
     2461 ctccggcggc cgctccgagg gccgcggcag cctgctgaca tgcggcgacg tggaagagaa
     2521 ccctggccct gccgctgcaa acctgctgac tgtgcaccaa aacctgcctg ccctccctgt
     2581 ggatgccacc tctgatgaag tcaggaagaa cctgatggac atgttcaggg acaggcaggc
     2641 cttctctgaa cacacctgga agatgctcct gtctgtgtgc agatcctggg ctgcctggtg
     2701 caagctgaac aacaggaaat ggttccctgc tgaacctgag gatgtgaggg actacctcct
     2761 gtacctgcaa gccagaggcc tggctgtgaa gaccatccaa cagcacctgg gccagctcaa
     2821 catgctgcac aggagatctg gcctgcctcg cccttctgac tccaatgctg tgtccctggt
     2881 gatgaggaga atcagaaagg agaatgtgga tgctggggag agagccaagc aggccctggc
     2941 ctttgaacgc actgactttg accaagtcag atccctgatg gagaactctg acagatgcca
     3001 ggacatcagg aacctggcct tcctgggcat tgcctacaac accctgctgc gcattgccga
     3061 aattgccaga atcagagtga aggacatctc ccgcaccgat ggtgggagaa tgctgatcca
     3121 cattggcagg accaagaccc tggtgtccac agctggtgtg gagaaggccc tgtccctggg
     3181 ggttaccaag ctggtggaga gatggatctc tgtgtctggt gtggctgatg accccaacaa
     3241 ctacctgttc tgccgggtca gaaagaatgg tgtggctgcc ccttctgcca cctcccaact
     3301 gtccacccgg gccctggaag ggatctttga ggccacccac cgcctgatct atggtgccaa
     3361 ggatgactct gggcagagat acctggcctg gtctggccac tctgccagag tgggtgctgc
     3421 cagggacatg gccagggctg gtgtgtccat ccctgaaatc atgcaggctg gtggctggac
     3481 caatgtgaac attgtgatga actacatcag aaacctggac tctgagactg gggccatggt
     3541 gaggctgcta gaggatgggg acctcgagcc atctgctgga gacatgagag ctgccaacct
     3601 ttggccaagc ccgctcatga tcaaacgctc taagaagaac agcctggcct tgtccctgac
     3661 ggccgaccag atggtcagtg ccttgttgga tgctgagccc cccatactct attccgagta
     3721 tgatcctacc agacccttca gtgaagcttc gatgatgggc ttactgacca acctggcaga
     3781 cagggagctg gttcacatga tcaactgggc gaagagggtg ccaggctttg tggatttgac
     3841 cctccatgat caggtccacc ttctagaatg tgcctggcta gagatcctga tgattggtct
     3901 cgtctggcgc tccatggagc acccagtgaa gctactgttt gctcctaact tgctcttgga
     3961 caggaaccag ggaaaatgtg tagagggcat ggtggagatc ttcgacatgc tgctggctac
     4021 atcatctcgg ttccgcatga tgaatctgca gggagaggag tttgtgtgcc tcaaatctat
     4081 tattttgctt aattctggag tgtacacatt tctgtccagc accctgaagt ctctggaaga
     4141 gaaggaccat atccaccgag tcctggacaa gatcacagac actttgatcc acctgatggc
     4201 caaggcaggc ctgaccctgc agcagcagca ccagcggctg gcccagctcc tcctcatcct
     4261 ctcccacatc aggcacatga gtaacaaagg catggagcat ctgtacagca tgaagtgcaa
     4321 gaacgtggtg cccctctatg acctgctgct ggaggcggcg gacgcccacc gcctacatgc
     4381 gcccactagc cgtggagggg catccgtgga ggagacggac caaagccact tggccactgc
     4441 gggctctact tcatcgcatt ccttgcaaaa gtattacatc acgggggagg cagagggttt
     4501 ccctgccaca gcttgaggcg cgccggatct ttttccctct gccaaaaatt atggggacat
     4561 catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt tcattgcaat
     4621 agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg caaatcattt
     4681 aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata tgctggctgc
     4741 catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     4801 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     4861 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     4921 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga gatccctcga
     4981 cctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     5041 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tgggtgccta
     5101 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     5161 cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     5221 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     5281 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     5341 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt
     5401 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     5461 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct
     5521 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     5581 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     5641 ctcaaaggcg gtaatacggt tatccacaga atcagggata acgcaggaaa gaacatgtga
     5701 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     5761 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     5821 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     5881 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     5941 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     6001 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     6061 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     6121 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6181 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6241 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6301 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6361 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     6421 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     6481 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6541 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     6601 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     6661 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     6721 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     6781 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     6841 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     6901 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     6961 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     7021 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     7081 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     7141 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     7201 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     7261 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     7321 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     7381 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     7441 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgc
//
