LOCUS       pMUT1s3AsG              6018 bp ds-DNA     circular     11-MAY-2018
DEFINITION  Escherichia coli Nissle 1917, complete genome.
ACCESSION   NZ_CP007799
VERSION     NZ_CP007799.1
KEYWORDS    .
SOURCE      .
  ORGANISM  . .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    4201..4946
                     /label=Homology to replication regions of plasmid pNTP1,pN
                     /ApEinfo_fwdcolor=#cfd5ff
                     /ApEinfo_revcolor=#c8c8c8
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5024..5473
                     /label=putitative oriT region
                     /ApEinfo_fwdcolor=#cfd5ff
                     /ApEinfo_revcolor=#c8c8c8
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2646..2651
                     /label=BsaI Golden recognition
                     /ApEinfo_fwdcolor=#ff1d12
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            complement(3052..3480)
                     /locus_tag="ECOLIN_RS25935"
                     /old_locus_tag="ECOLIN_25155"
                     /label=gene
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1493..1504
                     /label=BBa_B0034
                     /ApEinfo_fwdcolor=#c7ff9a
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(3052..3480)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /locus_tag="ECOLIN_RS25935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ECOLIN_25155"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000222531.1"
                     /transl_table=11
                     /label=helix-turn-helix domain-containing protein
                     /ApEinfo_fwdcolor=#b872ff
                     /ApEinfo_revcolor=#c01aff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1497..1500
                     /label=Conserved: 
                     /ApEinfo_fwdcolor=#c7ff9a
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1410..1449
                     /label=UNS9
                     /ApEinfo_fwdcolor=#c9fff9
                     /ApEinfo_revcolor=#73c4c4
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2228..2230
                     /label=BBa_E1010
                     /ApEinfo_fwdcolor=#c7ff9a
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            3772..4200
                     /locus_tag="ECOLIN_RS25940"
                     /old_locus_tag="ECOLIN_25160"
                     /label=gene(1)
                     /ApEinfo_label=gene
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             1511..2227
                     /label=sfGFP CDS
                     /ApEinfo_fwdcolor=#15ff0c
                     /ApEinfo_revcolor=#99ccff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      2753..2800
                     /note="T7 Terminator"
                     /label=T7 Terminator
                     /ApEinfo_fwdcolor=#3aff2a
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1450..1484)
                     /label=BBa_J23101
                     /ApEinfo_fwdcolor=#ffb114
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             3772..4200
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /locus_tag="ECOLIN_RS25940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ECOLIN_25160"
                     /product="hypothetical protein"
                     /protein_id="WP_000534558.1"
                     /transl_table=11
                     /label=hypothetical protein
                     /ApEinfo_fwdcolor=#b872ff
                     /ApEinfo_revcolor=#c01aff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2231..2255
                     /label=barcode: 
                     /ApEinfo_fwdcolor=#c7ff9a
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2264..2343
                     /label=BBa_B0010
                     /ApEinfo_fwdcolor=#c7ff9a
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            4457..4724
                     /locus_tag="ECOLIN_RS31090"
                     /pseudo=""
                     /label=gene(2)
                     /ApEinfo_label=gene
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     stem_loop       2275..2318
                     /label=BBa_B0010(1)
                     /ApEinfo_label=BBa_B0010
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2352..2392
                     /label=BBa_B0012
                     /ApEinfo_fwdcolor=#c7ff9a
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             4457..4724
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000844.1"
                     /locus_tag="ECOLIN_RS31090"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /label=hypothetical protein(1)
                     /ApEinfo_label=hypothetical protein
                     /ApEinfo_fwdcolor=#b872ff
                     /ApEinfo_revcolor=#c01aff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     stem_loop       2359..2378
                     /label=T7 TE
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     polyA_signal    2379..2392
                     /label=T7 TE(1)
                     /ApEinfo_label=T7 TE
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            complement(4664..4846)
                     /locus_tag="ECOLIN_RS29945"
                     /pseudo=""
                     /label=gene(3)
                     /ApEinfo_label=gene
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(270..1212)
                     /label=BBa_P1002
                     /ApEinfo_fwdcolor=#ff49e9
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3535..3536
                     /label=PETER INSERTED HERE (Site 1)
                     /ApEinfo_fwdcolor=#37e678
                     /ApEinfo_revcolor=#3de61d
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        300..305
                     /label=ampR -35
                     /ApEinfo_fwdcolor=#16fff9
                     /ApEinfo_revcolor=#acff1d
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        326..331
                     /label=ampR -10
                     /ApEinfo_fwdcolor=#16fff9
                     /ApEinfo_revcolor=#acff1d
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     RBS             340..344
                     /label=ampR RBS
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             349..1212
                     /gene="ampicillin resistance"
                     /label=ampR RBS(1)
                     /ApEinfo_label=ampR RBS
                     /ApEinfo_fwdcolor=#15ff0c
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1213..1219)
                     /label=BBa_G0004
                     /ApEinfo_fwdcolor=#ff49e9
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(4664..4846)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326445.1"
                     /locus_tag="ECOLIN_RS29945"
                     /note="incomplete; partial in the middle of a contig;
                     missing stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /label=hypothetical protein(2)
                     /ApEinfo_label=hypothetical protein
                     /ApEinfo_fwdcolor=#b872ff
                     /ApEinfo_revcolor=#c01aff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1220..1291)
                     /label=BBa_B0053
                     /ApEinfo_fwdcolor=#ff49e9
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     stem_loop       1228..1262
                     /label=BBa_B0053(1)
                     /ApEinfo_label=BBa_B0053
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1292..1311)
                     /label=BBa_G00100
                     /ApEinfo_fwdcolor=#ff49e9
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      2697..2815
                     /label=Tphi terminator
                     /ApEinfo_fwdcolor=#cfd5ff
                     /ApEinfo_revcolor=#c8c8c8
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1450..1455
                     /label=-35
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            complement(5035..5229)
                     /locus_tag="ECOLIN_RS25945"
                     /old_locus_tag="ECOLIN_25165"
                     /label=gene(4)
                     /ApEinfo_label=gene
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(1312..1388)
                     /label=BBa_B0055
                     /ApEinfo_fwdcolor=#ff49e9
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1..20
                     /label=r1
                     /ApEinfo_fwdcolor=#c9fff9
                     /ApEinfo_revcolor=#73c4c4
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            complement(1339..1377)
                     /gene="identical to bacteriophage T3"
                     /label=BBa_B0055(1)
                     /ApEinfo_label=BBa_B0055
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2826..2845
                     /label=r2
                     /ApEinfo_fwdcolor=#c9fff9
                     /ApEinfo_revcolor=#73c4c4
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1473..1478
                     /label=-10
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(5035..5229)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041622.1"
                     /locus_tag="ECOLIN_RS25945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ECOLIN_25165"
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041622.1"
                     /transl_table=11
                     /label=Rop family plasmid primer RNA-binding protein
                     /ApEinfo_fwdcolor=#b872ff
                     /ApEinfo_revcolor=#c01aff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     stem_loop       complement(1347..1368)
                     /label=stem loop from bacteriophage T3
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(46..52)
                     /label=BBa_G0006
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(53..124)
                     /label=BBa_B0053(2)
                     /ApEinfo_label=BBa_B0053
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            5513..5640
                     /locus_tag="ECOLIN_RS25950"
                     /old_locus_tag="ECOLIN_25170"
                     /label=gene(5)
                     /ApEinfo_label=gene
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     stem_loop       61..95
                     /label=BBa_B0053(3)
                     /ApEinfo_label=BBa_B0053
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(145..222)
                     /label=BBa_B0055(2)
                     /ApEinfo_label=BBa_B0055
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            complement(172..210)
                     /gene="identical to bacteriophage T3"
                     /label=BBa_B0055(3)
                     /ApEinfo_label=BBa_B0055
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     stem_loop       complement(180..201)
                     /label=stem loop from bacteriophage T3(1)
                     /ApEinfo_label=stem loop from bacteriophage T3
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(223..234)
                     /label=BBa_B0042
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(223..225)
                     /label=stop: stop codon (-1 frame)
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    224..226
                     /label=stop: stop codon (+2 frame)
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(227..229)
                     /label=stop: stop codon (-3 frame)
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             5513..5640
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /locus_tag="ECOLIN_RS25950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ECOLIN_25170"
                     /product="hypothetical protein"
                     /protein_id="WP_000166966.1"
                     /transl_table=11
                     /label=mobA
                     /ApEinfo_fwdcolor=#b872ff
                     /ApEinfo_revcolor=#c01aff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    228..230
                     /label=stop: stop codon (+3 frame)
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(231..233)
                     /label=stop: stop codon (-2 frame)
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    232..234
                     /label=stop: stop codon (+1 frame)
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(235..247)
                     /label=BBa_B0043
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            235..239
                     /gene="Topoisomerase I recognition site"
                     /label=BBa_B0043(1)
                     /ApEinfo_label=BBa_B0043
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            239..239
                     /gene="Generated T overhang"
                     /label=BBa_B0043(2)
                     /ApEinfo_label=BBa_B0043
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            240..242
                     /gene="Arbitrary sequence (could be any bases)"
                     /label=BBa_B0043(3)
                     /ApEinfo_label=BBa_B0043
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            complement(243..247)
                     /gene="Nt.BstNB I recognition site"
                     /label=BBa_B0043(4)
                     /ApEinfo_label=BBa_B0043
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             3052..3480
                     /label=putitative DNA binding protein
                     /ApEinfo_fwdcolor=#005bff
                     /ApEinfo_revcolor=#52dde5
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            248..269
                     /gene="Prefix"
                     /label=BBa_B0043(5)
                     /ApEinfo_label=BBa_B0043
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(248..269)
                     /label=BBa_G00000
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    248..267
                     /label=prefix
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             3772..4200
                     /label=Multispecies [Enterobacterales], Unknown function
                     /ApEinfo_fwdcolor=#005bff
                     /ApEinfo_revcolor=#52dde5
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     3247..3266
                     /label=Muta 6
                     /ApEinfo_fwdcolor=#ff0600
                     /ApEinfo_revcolor=#c80000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     2906..2925
                     /label=Muta 5
                     /ApEinfo_fwdcolor=#ff0600
                     /ApEinfo_revcolor=#c80000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    3625..3626
                     /label=INSERT HERE (Site 2)
                     /ApEinfo_fwdcolor=#30cc00
                     /ApEinfo_revcolor=#00ff28
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2846..2846
                     /label=INSERT HERE (Site 3)
                     /ApEinfo_fwdcolor=#30cc00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6018..6018
                     /label=INSERT HERE (Site 3)(1)
                     /ApEinfo_label=INSERT HERE (Site 3)
                     /ApEinfo_fwdcolor=#30cc00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            5641..5836
                     /locus_tag="ECOLIN_RS25950"
                     /old_locus_tag="ECOLIN_25170"
                     /label=gene(6)
                     /ApEinfo_label=gene
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             5641..5836
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /locus_tag="ECOLIN_RS25950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ECOLIN_25170"
                     /product="hypothetical protein"
                     /protein_id="WP_000166966.1"
                     /transl_table=11
                     /label=mobA(1)
                     /ApEinfo_label=mobA
                     /ApEinfo_fwdcolor=#b872ff
                     /ApEinfo_revcolor=#c01aff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2599..2638
                     /label=UNSX
                     /ApEinfo_fwdcolor=#9882ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2524..2563
                     /label=UNS1
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2572..2577
                     /label=BbsI golden recognition
                     /ApEinfo_fwdcolor=#ff160a
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    2593..2598
                     /label=AvrII
                     /ApEinfo_fwdcolor=#ff1129
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(2446..2486)
                     /label=BBa_B0062
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(2487..2493)
                     /label=BBa_G0005
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 CCCTAAATCT AGAATGCGGT cgagctcgta aacttggtct gacagctcta gctccggcaa
       61 aaaaacgggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa gattacttcg
      121 cgtttgccac ctgacgtcta agaaaaggaa tattcagcaa tttgcccgtg ccgaagaaag
      181 gcccacccgt gaaggtgagc cagtgagttg attgctacgt aattagttag ttagccctta
      241 gtgactcgaa ttcgcggccg cttctagagc taaatacatt caaatatcta tccgctcatg
      301 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagaatat gagtattcaa
      361 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      421 ccagaaacgc tggtgaaagt aaaagatgcc gaagatcagt tgggtgcacg tgtgggttac
      481 atcgaactgg acctcaacag cggtaagatt cttgagagtt ttcgccccga agaacgtttc
      541 ccaatgatga gcacttttaa agttctgctc tgtggcgcgg tattatcccg tattgacgcc
      601 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      661 ccagtcacag aaaagcatct tacggacggc atgacagtac gcgaattatg cagcgctgcc
      721 ataaccatga gtgataacac ggcggccaac ttacttctga caacgatcgg aggaccgaag
      781 gagcttaccg cttttttgca caacatgggt gatcatgtaa ctcgccttga tcgttgggaa
      841 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagctatg
      901 gcaacaacgt tgcgcaaact cttaactggc gaacttctta ctctcgcttc ccggcaacaa
      961 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1021 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtcccg cggtattatt
     1081 gcagccctgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagc
     1141 caggcaacta tggacgaacg taatcgccag atcgctgaga taggtgcctc cctgattaag
     1201 cattggtaat aatactagct ccggcaaaaa aacgggcaag gtgtcaccac cctgcccttt
     1261 ttctttaaaa ccgaaaagat tacttcgcgt ttgccacctg acgtctaaga aaaggaatat
     1321 tcagcaattt gcccgtgccg aagaaaggcc cacccgtgaa ggtgagccag tgagttgatt
     1381 gctacgtaac aatacgcaaa ccgcctctcG TTCCTTATCA TCTGGCGAAT CGGACCCAcA
     1441 AGAGCACTGT TTACAGCTAG CTCAGTCCTA GGTATTATGC TAGCtactag agaaagagga
     1501 gaaatactag atgcgtaaag gcgaagagct gttcactggt gtcgtcccta ttctggtgga
     1561 actggatggt gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc
     1621 aactaatggt aaactgacgc tgaagttcat ctgtactact ggtaaactgc cggtaccttg
     1681 gccgactctg gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt atccggacca
     1741 tatgaagcag catgacttct tcaagtccgc catgccggaa ggctatgtgc aggaacgcac
     1801 gatttccttt aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga
     1861 taccctggta aaccgcattg agctgaaagg cattgacttt aaagaagacg gcaatatcct
     1921 gggccataag ctggaataca attttaacag ccacaatgtt tacatcaccg ccgataaaca
     1981 aaaaaatggc attaaagcga attttaaaat tcgccacaac gtggaggatg gcagcgtgca
     2041 gctggctgat cactaccagc aaaacactcc aatcggtgat ggtcctgttc tgctgccaga
     2101 caatcactat ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga aacgcgatca
     2161 tatggttctg ctggagttcg taaccgcagc gggcatcacg catggtatgg atgaactgta
     2221 caaatgataa cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa
     2281 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     2341 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagta
     2401 gcggccgctg cggcgagcgg tatcagctca ctcaaaggcg gtaatcagat aaaaaaaatc
     2461 cttagctttc gctaaggatg atttctgcta gtaCGTACGG TTTCGAAGCT TGCTCTTCTT
     2521 AAGCATTACT CGCATCCATT CTCAGGCTGT CTCGTCTCGT CTCCGCGCGC GGTCTTCCTG
     2581 GAGATGATCA AACCTAGGCC AGGATACATA GATTACCACA ACTCCGAGCC CTTCCACCCG
     2641 CCGGCGAGAC CTCTAGGCCG CACTCGAGCA CCACCACCAC CACCACTGAG ATCCGGCTGC
     2701 TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA
     2761 ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC
     2821 CGGATAAACC TTGCTTCTTC GCGGTGtttc agtggtgcgt acaattaagg gattatggta
     2881 aatatatgag ctatgcgata actttaactg tgaagcgatg aacccattac aggcaaagcc
     2941 aattactcct gacagtggtt tagccagaag cagggctacc aagaccaatg caataagtaa
     3001 tatatcgttt tgctatcgtg catcgctcgc gctcagttcc attgtgcttt tTTAAGCTGT
     3061 CGTTTTTCTT ACGGTATATA CCGGTTTTTT ATGGCGTGGT TTCTTAACTT GTTCAGCTAC
     3121 TGATGGACCC ATTGTATCTA GGTAGTCAAC TAGCTTTGTT AGATCATAAA TATTGCGACC
     3181 ACCATATCGG CGATCACTCT TCGATGTTGG TTTCTTGTCC AAGAGATTAG CTTTTTCAAG
     3241 ATCATTGATA GCTCTCTGAA CAGTCCGTAC AGAAACCCCC ATACGTATGG CTAGACTTTC
     3301 CATTGACGGA TGCGGCCACT CTTGCAAACT CCACCAGTGA ACGATCAGGT TAAGTAGTGT
     3361 GTTAAAGGCC ACTGAAGTTA GCTTTTTCTC GTTTTGTATA AAAAACAATA CGGTAGGCAC
     3421 TGCTGTCCAG CCAAGAGACA AACCGCCAGC TTTCCATTTA TTCTTAACGG AGTAAGTCAT
     3481 tgattttcct aagccccaaa atatttaaag tatatattat atggtatatt cataTGaata
     3541 gggtgacact ggcgccatta ttgtgcaacc aaaaaagact actctgaaaa cgaggaaaag
     3601 attttttcct gcctgaatta gataCGgagt tagcgatatg aaaaccgaac aacgtcatga
     3661 tcttgttaaa gatattgagg tttttggcgt atccttgtct ctgttgattt ccagagcgaa
     3721 tgagaagtct gttacaatgc catctggtct aagtcgggag cagagaagag cATGGGCAGC
     3781 GGAGCAGGCG CGCAAAATCC ACAATTGAAT ATTGTCTCAT TCTCTGAGAC CTTCAACCTT
     3841 TATTACACAT CCAGATATTC TGCAAAAACA CTCGATAAAA TCGATGATTT CATTGAGCAT
     3901 TTTGAAAAAT ACAATCTCTT TGGCGATCCT TTAAAAGGAT ATCCAGCTTG GACTGGCAAA
     3961 GTATCGCCAT CGTGGAAAGT GCCTGATCAT TACGAAAACA AAGAAGCTAT TGAGAAGTAT
     4021 GCTAGAGCTA ACAAATTATG GCATGCTCAT TTAGGCGATC CGGTTTTTAA AGATACGTTT
     4081 CATGGGAAAT ACAAGGTTTC TGACTGGGTT ATTCATTTCC AGCGGCTGAC ACCGAACCAT
     4141 ATAAAGCTTT TAGAGCTTGG ATACCATGAC CCAATGAAGC TACCTCAAAA CTTTGAATGA
     4201 tcgagcggca ggctaaatga aatcttgaga ttcattcagt ctcgtcgtaa tctcactatt
     4261 gtaaaaacga aaaaaccacc ctggcaggtg gtttttcgaa ggttagttaa tcctggcaga
     4321 ttctctaacc gtggtaacag tcttgtgcga gacatgtcac caaatactgt cctttcagtg
     4381 tagcctcagt taggccgcca cttcaagaac tctcgttaca tctctcgcac atcctgctta
     4441 ccagtggccg ttgccaGTGG CGTTAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG
     4501 TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG
     4561 GAGCGAACGA CCTACACCGA ACCGAGATAC CAACAGCGTG AGCTATGAGA AAGCGCCACG
     4621 CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG
     4681 CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC
     4741 CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA
     4801 AACGCCTCCC GCGCGCCCTT CTTCTGGGTC TTTGTCTTTT GCTCACatgt tctttccggt
     4861 tttatccccc gattctgtgg ataaccgtat taccgccttt gagtgagctg acaccgctcg
     4921 ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaag agcgccttat
     4981 gtgacatttt ctccttacgc tcctctatgc cgttctgcat cctgtccgga tgcgTTATAT
     5041 CCCGGTAAGA TTTTCCGCTT CAAAGCGTGT CTGTATGCTG TTCTGGAGTT CTTCTGCGAG
     5101 TTCGTGCAGT TTCTCACACA TGGCGGCCTG TTCGTCGGCA TTGAGTGCGT CCAGTTTTTC
     5161 GAGCAGCGTC AGGCTCTGAC TTTTTATGAA TCCCGCCATG TTGAGTACGG CTTGCTGCTG
     5221 CTTATTCATc ttttcgtttt ctccgttctg tctgtcatct gcgttgtgtg attatatcgc
     5281 gtaccacttt tcgactgttt tgctgccgct attctgccgc ttggcttttt gacgggcatt
     5341 tctgtcagac aacactgtca ctgccaaaaa actgccgtgc ctttgtcggt aattcgagct
     5401 tgctgacagg acaggatgtg caattgttat accgcgcata catgcacgct attacaattg
     5461 ccctggtcag ggctttgccc cgacacccat gtcagatacg gagccatgtt ttATGACAAA
     5521 ACGAAGTGGA AGTAATACGC GCAGGCGGGC TATCAGTCGC CCTGTTCGTC TGACGGCAGA
     5581 AGAAGACCAG GAAATCAGAA AAAGGGCTGC TGAATGCGGC AAGACCGTTT CCGGTTTTTT
     5641 ACGGGCGGCA GCTCTCGGTA AGAAAGTTAA CTCACTGACT GATGATCGGG TACTGAAAGA
     5701 AGTTATGAGA CTGGGGGCGT TACAGAAAAA ACTCTTTATC GACGGCAAGC GTGTCGGGGA
     5761 CAGGGAGTAT GCGGAGGTGC TGATCGCTAT TACGGAGTAT CACCGTGCCC TGTTATCCAG
     5821 GCTTATGGCA GATTAGcttc ccggagagaa aactgtcgaa aactgacggt atgaacaccg
     5881 taagctccca aagtgatcac cattcgcttt catgcatagc tatgcagcga gctgaaaacg
     5941 atcctgacgc atccttcctg tttttccggg gtaaaacatc tctttttgcg gtgtctgcgt
     6001 cagaatcgcg ttcagcgC    
//
