LOCUS       pMUT2s2AsR_TS          10925 bp ds-DNA     circular     12-DEC-2019
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     gene            complement(2120..2353)
                     /locus_tag="ECOLIN_RS25900"
                     /old_locus_tag="ECOLIN_25125"
                     /label=gene
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    9197..9236
                     /label=UNSX
                     /ApEinfo_fwdcolor=#9882ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(2120..2353)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554708.1"
                     /locus_tag="ECOLIN_RS25900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ECOLIN_25125"
                     /product="hypothetical protein"
                     /protein_id="WP_001554708.1"
                     /transl_table=11
                     /label=hypothetical protein
                     /ApEinfo_fwdcolor=#b872ff
                     /ApEinfo_revcolor=#c01aff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(4053..4077)
                     /label=BBa_I50041
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     RBS             6897..6902
                     /note="ribosome binding site"
                     /label=ribosome binding site
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            complement(10686..10925)
                     /locus_tag="ECOLIN_RS25880"
                     /old_locus_tag="ECOLIN_25105"
                     /label=gene(1)
                     /ApEinfo_label=gene
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            complement(1..408)
                     /locus_tag="ECOLIN_RS25880"
                     /old_locus_tag="ECOLIN_25105"
                     /label=gene(2)
                     /ApEinfo_label=gene
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5422..5441
                     /label=BBa_R0010
                     /ApEinfo_fwdcolor=#c7ff9a
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            1791..2075
                     /locus_tag="ECOLIN_RS25905"
                     /old_locus_tag="ECOLIN_25130"
                     /label=gene(3)
                     /ApEinfo_label=gene
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8440..8440
                     /note="L208Q"
                     /note="TlpA 36 mutation"
                     /label=L208Q
                     /ApEinfo_fwdcolor=#e60c1a
                     /ApEinfo_revcolor=#e60c1a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(10686..10925)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /locus_tag="ECOLIN_RS25880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /old_locus_tag="ECOLIN_25105"
                     /product="hypothetical protein"
                     /protein_id="WP_024199421.1"
                     /transl_table=11
                     /label=hypothetical protein(1)
                     /ApEinfo_label=hypothetical protein
                     /ApEinfo_fwdcolor=#b872ff
                     /ApEinfo_revcolor=#c01aff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(1..408)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS+"
                     /locus_tag="ECOLIN_RS25880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS+."
                     /old_locus_tag="ECOLIN_25105"
                     /product="hypothetical protein"
                     /protein_id="WP_024199421.1"
                     /transl_table=11
                     /label=hypothetical protein(2)
                     /ApEinfo_label=hypothetical protein
                     /ApEinfo_fwdcolor=#b872ff
                     /ApEinfo_revcolor=#c01aff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             1791..2075
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554709.1"
                     /locus_tag="ECOLIN_RS25905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ECOLIN_25130"
                     /product="RelE/StbE family addiction module toxin"
                     /protein_id="WP_001554709.1"
                     /transl_table=11
                     /label=RelE/StbE family addiction module toxin
                     /ApEinfo_fwdcolor=#b872ff
                     /ApEinfo_revcolor=#c01aff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_signal     6816..6887
                     /label=riboJ
                     /ApEinfo_fwdcolor=#cdff0a
                     /ApEinfo_revcolor=#ffcd33
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            6386..6406
                     /gene="Suffix"
                     /label=Suffix
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    9244..9249
                     /label=BsaI Golden recognition
                     /ApEinfo_fwdcolor=#ff1d12
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6680..6719
                     /label=UNS4
                     /ApEinfo_fwdcolor=#ff6155
                     /ApEinfo_revcolor=#ff7b0a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            complement(9541..10683)
                     /locus_tag="ECOLIN_RS25885"
                     /old_locus_tag="ECOLIN_25110"
                     /label=gene(4)
                     /ApEinfo_label=gene
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            complement(6415..6415)
                     /gene="Generated T overhang"
                     /label=BBa_B0044
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    9157..9196
                     /label=UNS5
                     /ApEinfo_fwdcolor=#30cc00
                     /ApEinfo_revcolor=#3cff00
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            1560..1801
                     /locus_tag="ECOLIN_RS25910"
                     /old_locus_tag="ECOLIN_25135"
                     /label=gene(5)
                     /ApEinfo_label=gene
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5442..5481
                     /label=UNS9
                     /ApEinfo_fwdcolor=#c9fff9
                     /ApEinfo_revcolor=#73c4c4
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(9541..10683)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704287.1"
                     /locus_tag="ECOLIN_RS25885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ECOLIN_25110"
                     /product="DUF4868 domain-containing protein"
                     /protein_id="WP_001704287.1"
                     /transl_table=11
                     /label=DUF4868 domain-containing protein
                     /ApEinfo_fwdcolor=#b872ff
                     /ApEinfo_revcolor=#c01aff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     source          8934..9095
                     /organism="synthetic DNA construct"
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /label=source:synthetic DNA construct
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8934..9024
                     /note="MCS-2"
                     /note="multiple cloning site #2"
                     /label=MCS-2
                     /ApEinfo_fwdcolor=#e60c1a
                     /ApEinfo_revcolor=#e60c1a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     stem_loop       6442..6473
                     /label=stem loop
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      9100..9156
                     /label=L3S2P56
                     /ApEinfo_fwdcolor=#cfd5ff
                     /ApEinfo_revcolor=#c8c8c8
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(5497..5516)
                     /label=BBa_J23101
                     /ApEinfo_fwdcolor=#ffb114
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             8952..8996
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic
                     ribonuclease A"
                     /note="S-Tag"
                     /translation="KETAAAKFERQHMDS"
                     /label=affinity and epitope tag derived from pancreatic  
                     /ApEinfo_fwdcolor=#b872ff
                     /ApEinfo_revcolor=#c01aff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(6603..6609)
                     /label=BBa_G0005
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             1560..1801
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554710.1"
                     /locus_tag="ECOLIN_RS25910"
                     /note="Qin prophage; bifunctional antitoxin of the
                     RelE-RelB toxin-antitoxin system/transcriptional
                     repressor; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /old_locus_tag="ECOLIN_25135"
                     /product="type II toxin-antitoxin system antitoxin,
                     RelB/DinJ family"
                     /protein_id="WP_001554710.1"
                     /transl_table=11
                     /label=type II toxin-antitoxin system antitoxin, RelB/Din
                     /ApEinfo_fwdcolor=#005bff
                     /ApEinfo_revcolor=#52dde5
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      9351..9398
                     /note="T7 Terminator"
                     /label=T7 Terminator
                     /ApEinfo_fwdcolor=#3aff2a
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            join(3853..4032,9444..9524)
                     /locus_tag="ECOLIN_RS29930"
                     /label=gene(6)
                     /ApEinfo_label=gene
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      9048..9095
                     /note="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
                     /label=T7 terminator
                     /ApEinfo_fwdcolor=#cfd5ff
                     /ApEinfo_revcolor=#c8c8c8
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            complement(981..1294)
                     /locus_tag="ECOLIN_RS25915"
                     /old_locus_tag="ECOLIN_25140"
                     /label=gene(7)
                     /ApEinfo_label=gene
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(5482..5496)
                     /label=BBa_J23101(1)
                     /ApEinfo_label=BBa_J23101
                     /ApEinfo_fwdcolor=#ffb114
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             join(3853..4032,9444..9524)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360352.1"
                     /locus_tag="ECOLIN_RS29930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_071925566.1"
                     /transl_table=11
                     /label=hypothetical protein(3)
                     /ApEinfo_label=hypothetical protein
                     /ApEinfo_fwdcolor=#b872ff
                     /ApEinfo_revcolor=#c01aff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             6910..6912
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
                     /label=start codon
                     /ApEinfo_fwdcolor=#b872ff
                     /ApEinfo_revcolor=#c01aff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(6386..6406)
                     /label=BBa_G00001
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             6952..7662
                     /codon_start=1
                     /product="green variant of teal fluorescent protein mTFP1
                     (Ai et al., 2008)"
                     /note="mWasabi"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYD
                     G
                     TNTINLEVKEGAPLPFSYDILTTAFSYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMT
                     F
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKETTGWDASTERMYVRDGVL
                     K
                     GDVKMKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYEI
                     A VARNSTDGMDELYKG"
                     /label=green variant of teal fluorescent protein mTFP1  (
                     /ApEinfo_fwdcolor=#b872ff
                     /ApEinfo_revcolor=#c01aff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            6407..6411
                     /gene="Nt.BstNB I recognition site"
                     /label=BBa_G00001(1)
                     /ApEinfo_label=BBa_G00001
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     RBS             7804..7809
                     /note="ribosome binding site"
                     /label=ribosome binding site(1)
                     /ApEinfo_label=ribosome binding site
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        6740..6791
                     /bound_moiety="TlpA"
                     /note="TlpA Promoter"
                     /label=TlpA Promoter E10 variant
                     /ApEinfo_fwdcolor=#ff0600
                     /ApEinfo_revcolor=#e60c1a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(6407..6419)
                     /label=BBa_B0044(1)
                     /ApEinfo_label=BBa_B0044
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    7996..7996
                     /note="P60L"
                     /note="TlpA 36 mutation"
                     /label=P60L
                     /ApEinfo_fwdcolor=#e60c1a
                     /ApEinfo_revcolor=#e60c1a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5525..5536
                     /label=BBa_B0034
                     /ApEinfo_fwdcolor=#c7ff9a
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(981..1294)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674370.1"
                     /locus_tag="ECOLIN_RS25915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ECOLIN_25140"
                     /product="replicase"
                     /protein_id="WP_024199422.1"
                     /transl_table=11
                     /label=replicase
                     /ApEinfo_fwdcolor=#b872ff
                     /ApEinfo_revcolor=#c01aff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            6412..6414
                     /gene="Arbitrary sequence (could be any bases)"
                     /label=BBa_B0044(2)
                     /ApEinfo_label=BBa_B0044
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            complement(3577..3903)
                     /locus_tag="ECOLIN_RS25890"
                     /old_locus_tag="ECOLIN_25115"
                     /label=gene(8)
                     /ApEinfo_label=gene
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5529..5532
                     /label=Conserved: 
                     /ApEinfo_fwdcolor=#c7ff9a
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    5543..6223
                     /label=BBa_E1010
                     /ApEinfo_fwdcolor=#c7ff9a
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            complement(6415..6419)
                     /gene="Topoisomerase I recognition site"
                     /label=BBa_B0044(3)
                     /ApEinfo_label=BBa_B0044
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             5543..6217
                     /gene="mrfp1"
                     /label=BBa_E1010 mRFP1
                     /ApEinfo_fwdcolor=#ff081a
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(4302..5244)
                     /label=BBa_P1002
                     /ApEinfo_fwdcolor=#ff49e9
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(6420..6431)
                     /label=BBa_B0042
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        4332..4337
                     /label=ampR -35
                     /ApEinfo_fwdcolor=#16fff9
                     /ApEinfo_revcolor=#acff1d
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(3577..3903)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001715145.1"
                     /locus_tag="ECOLIN_RS25890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ECOLIN_25115"
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_001554615.1"
                     /transl_table=11
                     /label=plasmid mobilization relaxosome protein MobC
                     /ApEinfo_fwdcolor=#b872ff
                     /ApEinfo_revcolor=#c01aff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6224..6248
                     /label=barcode: 
                     /ApEinfo_fwdcolor=#c7ff9a
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        4358..4363
                     /label=ampR -10
                     /ApEinfo_fwdcolor=#16fff9
                     /ApEinfo_revcolor=#acff1d
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     RBS             4372..4376
                     /label=ampR RBS
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6257..6336
                     /label=BBa_B0010
                     /ApEinfo_fwdcolor=#c7ff9a
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             4381..5244
                     /gene="ampicillin resistance"
                     /label=ampR RBS(1)
                     /ApEinfo_label=ampR RBS
                     /ApEinfo_fwdcolor=#15ff0c
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(5245..5251)
                     /label=BBa_G0004
                     /ApEinfo_fwdcolor=#ff49e9
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(5252..5323)
                     /label=BBa_B0053
                     /ApEinfo_fwdcolor=#ff49e9
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     stem_loop       6268..6311
                     /label=BBa_B0010(1)
                     /ApEinfo_label=BBa_B0010
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     stem_loop       5260..5294
                     /label=BBa_B0053(1)
                     /ApEinfo_label=BBa_B0053
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(5324..5343)
                     /label=BBa_G00100
                     /ApEinfo_fwdcolor=#ff49e9
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     terminator      9295..9413
                     /label=Tphi terminator
                     /ApEinfo_fwdcolor=#cfd5ff
                     /ApEinfo_revcolor=#c8c8c8
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6640..6659
                     /label=UNS1
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            complement(2943..3629)
                     /locus_tag="ECOLIN_RS29935"
                     /pseudo=""
                     /label=gene(9)
                     /ApEinfo_label=gene
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(5344..5420)
                     /label=BBa_B0055
                     /ApEinfo_fwdcolor=#ff49e9
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6660..6679
                     /label=UNS1(1)
                     /ApEinfo_label=UNS1
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(2943..3629)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:PF03432.12"
                     /locus_tag="ECOLIN_RS29935"
                     /note="incomplete; partial in the middle of a contig;
                     missing stop; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /product="relaxase"
                     /pseudo=""
                     /transl_table=11
                     /label=relaxase
                     /ApEinfo_fwdcolor=#b872ff
                     /ApEinfo_revcolor=#c01aff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4033..4052
                     /label=r1
                     /ApEinfo_fwdcolor=#c9fff9
                     /ApEinfo_revcolor=#73c4c4
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6345..6385
                     /label=BBa_B0012
                     /ApEinfo_fwdcolor=#c7ff9a
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            complement(2414..2899)
                     /locus_tag="ECOLIN_RS25895"
                     /old_locus_tag="ECOLIN_25120"
                     /label=gene(10)
                     /ApEinfo_label=gene
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            complement(5371..5409)
                     /gene="identical to bacteriophage T3"
                     /label=BBa_B0055(1)
                     /ApEinfo_label=BBa_B0055
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    9424..9443
                     /label=r2
                     /ApEinfo_fwdcolor=#c9fff9
                     /ApEinfo_revcolor=#73c4c4
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(6432..6500)
                     /label=BBa_B0054
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     stem_loop       complement(5379..5400)
                     /label=stem loop from bacteriophage T3
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     4157..4176
                     /label=VF2
                     /ApEinfo_fwdcolor=#a3a5ff
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     stem_loop       6352..6371
                     /label=T7 TE
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(4078..4084)
                     /label=BBa_G0006
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(4085..4156)
                     /label=BBa_B0053(2)
                     /ApEinfo_label=BBa_B0053
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            6435..6474
                     /gene="identical to E. coli MG1655 between tonB and yciA"
                     /label=BBa_B0054(1)
                     /ApEinfo_label=BBa_B0054
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     stem_loop       4093..4127
                     /label=BBa_B0053(3)
                     /ApEinfo_label=BBa_B0053
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(2414..2899)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:PF04837.10"
                     /locus_tag="ECOLIN_RS25895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="ECOLIN_25120"
                     /product="hypothetical protein"
                     /protein_id="WP_001554707.1"
                     /transl_table=11
                     /label=hypothetical protein(4)
                     /ApEinfo_label=hypothetical protein
                     /ApEinfo_fwdcolor=#b872ff
                     /ApEinfo_revcolor=#c01aff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     polyA_signal    6372..6385
                     /label=T7 TE(1)
                     /ApEinfo_label=T7 TE
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(4177..4254)
                     /label=BBa_B0055(2)
                     /ApEinfo_label=BBa_B0055
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     2230..2249
                     /label=Muta 9
                     /ApEinfo_fwdcolor=#ff0600
                     /ApEinfo_revcolor=#c80000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            complement(4204..4242)
                     /gene="identical to bacteriophage T3"
                     /label=BBa_B0055(3)
                     /ApEinfo_label=BBa_B0055
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    6378..6378
                     /label=stop: 
                     /ApEinfo_fwdcolor=#c7ff9a
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     stem_loop       complement(4212..4233)
                     /label=stem loop from bacteriophage T3(1)
                     /ApEinfo_label=stem loop from bacteriophage T3
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(4255..4266)
                     /label=BBa_B0042(1)
                     /ApEinfo_label=BBa_B0042
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(4255..4257)
                     /label=stop: stop codon (-1 frame)
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4256..4258
                     /label=stop: stop codon (+2 frame)
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(6562..6602)
                     /label=BBa_B0062
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(4259..4261)
                     /label=stop: stop codon (-3 frame)
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4260..4262
                     /label=stop: stop codon (+3 frame)
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(4263..4265)
                     /label=stop: stop codon (-2 frame)
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4264..4266
                     /label=stop: stop codon (+1 frame)
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     2524..2543
                     /label=Muta 10
                     /ApEinfo_fwdcolor=#ff0600
                     /ApEinfo_revcolor=#c80000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(4267..4279)
                     /label=BBa_B0043
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1960..1979
                     /label=Muta 7
                     /ApEinfo_fwdcolor=#ff3900
                     /ApEinfo_revcolor=#e60c1a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            4267..4271
                     /gene="Topoisomerase I recognition site"
                     /label=BBa_B0043(1)
                     /ApEinfo_label=BBa_B0043
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     primer_bind     1551..1570
                     /label=Muta 8
                     /ApEinfo_fwdcolor=#e60c1a
                     /ApEinfo_revcolor=#ff3900
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            4271..4271
                     /gene="Generated T overhang"
                     /label=BBa_B0043(2)
                     /ApEinfo_label=BBa_B0043
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            4272..4274
                     /gene="Arbitrary sequence (could be any bases)"
                     /label=BBa_B0043(3)
                     /ApEinfo_label=BBa_B0043
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            complement(4275..4279)
                     /gene="Nt.BstNB I recognition site"
                     /label=BBa_B0043(4)
                     /ApEinfo_label=BBa_B0043
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             6922..6939
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /note="6xHis"
                     /translation="HHHHHH"
                     /label=6xHis affinity tag
                     /ApEinfo_fwdcolor=#b872ff
                     /ApEinfo_revcolor=#c01aff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc            4280..4301
                     /gene="Prefix"
                     /label=BBa_B0043(5)
                     /ApEinfo_label=BBa_B0043
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     promoter        7719..7796
                     /gene="lacI"
                     /note="lacI promoter"
                     /label=lacI
                     /ApEinfo_fwdcolor=#ff0600
                     /ApEinfo_revcolor=#e60c1a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(4280..4301)
                     /label=BBa_G00000
                     /ApEinfo_fwdcolor=#ff0514
                     /ApEinfo_revcolor=#f3ffae
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             7818..8933
                     /codon_start=1
                     /product="Evolved TlpA Expression starts at ~34C
                     Expression midpoint at ~37C Expression max at ~43C Refer
                     to data from September 30 2015  Mutations from wt:  P60L,
                     D135V, K187R, K202I, L208Q  Formerly known as TlpA Mutant
                     30"
                     /note="mTlpA1"
                     /translation="MRPATYEPEQIIEAGLALQAEGRNITGFALRNQVGGGNPTRLRQ
                     I
                     WDEYQASQSTVVTELVAELPVEVAEEVKAVSAALSERITQLATELNDKAVRAAERRVA
                     E
                     VTRAAGEQTAQAERELADAAQTVDDLEEKLVELQDRYDSLTLALESERSLRQQHDVEM
                     A
                     QLKERLAAAEENTRQREERYQEQRTVLQDALNAEQAQHINTREDQQKRLEQISAEANA
                     R
                     TEELKSERDKVNTLLTRLESQENALASERQQHLATRETLQQRLEQAIADTQARAGEIA
                     L
                     ERDRVSSLTARLESQEKASSEQLVRMGSEIASLTERCTQLENQRDDARLETMGEKETV
                     A ALRGEAEALKRQNQSLMAALSGNKQTGGQNA"
                     /label=Evolved TlpA Expression starts at ~34C Expression 
                     /ApEinfo_fwdcolor=#b872ff
                     /ApEinfo_revcolor=#c01aff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    4280..4299
                     /label=prefix
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8221..8221
                     /note="D135V"
                     /note="TlpA 36 Mutation"
                     /label=D135V
                     /ApEinfo_fwdcolor=#e60c1a
                     /ApEinfo_revcolor=#e60c1a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    1428..1429
                     /label=Insertion site s1
                     /ApEinfo_fwdcolor=#30cc00
                     /ApEinfo_revcolor=#00ff28
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8377..8377
                     /note="K187R"
                     /note="TlpA 36 Mutation"
                     /label=K187R
                     /ApEinfo_fwdcolor=#e60c1a
                     /ApEinfo_revcolor=#e60c1a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     gene            complement(398..980)
                     /locus_tag="ECOLIN_RS25875"
                     /label=gene(11)
                     /ApEinfo_label=gene
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    8422..8422
                     /note="K202I"
                     /note="TlpA 36 Mutation"
                     /label=K202I
                     /ApEinfo_fwdcolor=#e60c1a
                     /ApEinfo_revcolor=#e60c1a
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     CDS             complement(398..980)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342226.1"
                     /locus_tag="ECOLIN_RS25875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_077769348.1"
                     /transl_table=11
                     /label=hypothetical protein(5)
                     /ApEinfo_label=hypothetical protein
                     /ApEinfo_fwdcolor=#b872ff
                     /ApEinfo_revcolor=#c01aff
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    join(4032..4032,9444..9444)
                     /label=Insertion site s2
                     /ApEinfo_fwdcolor=#30cc00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
     misc_feature    complement(10853..10854)
                     /label=Insertion site 3
                     /ApEinfo_fwdcolor=#30cc00
                     /ApEinfo_revcolor=green
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
ORIGIN
        1 aacaaacaaa aaattaacta tatttgatgg tttgcttaaa tcagtaaaga ccaacggcat
       61 tatgtacgtt gataaaaaag aaagatactc accggattct tttttacatg aaacgacctt
      121 taactttctt gacaccgcac cggagtctaa gtttttcaaa acccatcgat accaaatgta
      181 tgtataagaa caagttaaaa tcaaagcccc gcagatcact gacctcaata cagaaaatgt
      241 taatctgcta tttgaatagt cgagtacgca ttgaaatttt ccatccgcgc cagaacacga
      301 agacatggcc ttatctaaaa cagaccatac gttatcaata ccagaaaaat atattgttat
      361 cggtataaat aaaaacaaca ttgataagag atacattcta attttcattt ttgtaaaatt
      421 tcctgtacca cgttgatcta cttattccta aagaaatcca ttctccatct ctaactttcg
      481 gccttccacc accagagctt ttttttccac gttgacgctg aatttcagaa gtatgtgttt
      541 gtttaacata ctcttcaaag ccaagctctg taaggttctt acttgtccac ttagccacac
      601 ttttagcaat tcccatgact tcgttctcgt ctaaaggtgc ggagaactgc aggttgtagg
      661 ctttagcgcg ttcaatgcag gcttgtagcc attggtcata ctgcggccag ccttggcgga
      721 tagcgcggta agcccacttg cgggttttat cgaagagggt gcagttacgg cctaaaccgt
      781 agtccggcag gatttcgcgg tcattggctg cgccaaggtc gaggtaatcg gctaaccagt
      841 caagggtata gagctctggc tgccagacgg tgatctgcca gtgcaggtgg ttcggattct
      901 tgcaaattag ccctgaatac cccgcatctg cgcccaattt tttacgcagc gcattctcga
      961 tggcggcggc gtatttaagg ggggcagctc gaccatccgg cgcggtacgt accgccgtat
     1021 gcaaggcata caacaggtga gcatgtccgt tctcggggtt tttgatggtg agtgtgggcg
     1081 caggtgcccc cagatcggcc caatcaatcg cggctccggc tctgtccacg tcaaagcaaa
     1141 gccagtacat ggcgtgaggc tgattaaact ggatgtattt tgcgaggaga gcacgctctt
     1201 taccggcaat gcgaacacca aactgtaaat catcggagaa gtacggcttg tggggtaacc
     1261 ggtcgttaaa aagcgttaaa gcctgattat ccaaggctcc cagccttatg gcggggctgt
     1321 tgttttgcac gctgcatgtg ctaatatcct ttctaggttt cgacctagcc ctgaatgtca
     1381 tgtccgctcg ccaaagtaga gcatgatttc ggggctttgt tttttctGCc actaagttac
     1441 acctcaacaa cggtttttgt catccccgac aatccgttat tcctgcttgt tctcgcacgg
     1501 ctttacgctc atactacttc ttgtagatac acttgtcact acatcaagag gtgagatgat
     1561 ggccacgatt aatattcgga tcgatgacga gctgaaaagc cgctcttatg ccgcactgga
     1621 aaagctgggc gtaacgccgt ccgaggttct gcgccaaaca ctggaatatg tggcccaaag
     1681 cggacgtttg ccgttccagc aggttttgct gaccgaggat gatgccgatt tgatggctat
     1741 cgttcgggat cgtctggaaa acccacaggc gggcgtaaag gtgtcactgg atgagctata
     1801 accttgaatt tgatccccga gccctgaagg aatggcgcaa gctcggggat gatgtccgtc
     1861 tgcagttcaa gaaaaaactc gagcaggttc tacaacaccc gcggatcgat aaaaatcgcc
     1921 tgcgagagct gcatgactgc tacaaaatca agctccgtgc atccggttat cgcttggtct
     1981 atcaggttcg cgatcaaacc attacggtat tcgtggtggc ggtcggtaag cgcgagcgtt
     2041 ctgccgctta cgatgcggcc gataaacgct tataaactca tgccgtgacc gcgagaatac
     2101 cgctgttcgt gccgcttggc taattgctca agcggcgcac tgttctgttt aagctctcga
     2161 cttgctgcgc caagccggtg acttgcatgc tcaaggcctg acaaaccctg tcgtttttcg
     2221 ccaactcctg cgaggtaacc tcgaacatgc gctgtaagtt ggcgtagctg tcctgccacg
     2281 cttgctgctg ttgttcgtag tgcctctgta agctctctaa tgcgctcaga agctgctgct
     2341 ccatttcggt catgaatctc ttcaccctga tagataaaac cgcccagaat cgattctgtg
     2401 gcgtctgatg aggttatttg gcgctgtact tgatgacctg acgatgttga gcgttcttgt
     2461 actcgtcgat cttcttcgcc ccctgcggaa ggatcaggta atacacgctc ttgttcttgg
     2521 aatcgtgaat tatcgatacg ccggctccgg tctggctctt taagtcctgc aggatctggc
     2581 tctgctcgct gatttcgttc tggcgttcga ccacgatagt cccgagatac caagctactc
     2641 caatcaatat cgcaaacagg atcccactta atgccaggct gtacagccat gtcattccga
     2701 ctaagcggtg tatctgtttt agctggctgt cgttctcttc ttggatagcg gtctgtatgt
     2761 tccctgagct taatttcaag gcctcggtga tacgtttctc gtgcttctcg aatgcgttcg
     2821 cgacgctcgt tgcggtagtc ttggcttgct gcttcgattt gctctcgaac tcccgcgcta
     2881 aatttaaaat ctcgctcata cagcactcct tttaagcgaa tattcgggcc acctgccgga
     2941 tcagcaatac tgatactgga tttggtttcc cgtacgaccg acaatccggc atcggtaagg
     3001 tgggaaatca cccctttacg atccgtaatt tctccctgct caatcaagct gattagccct
     3061 ttggtaatgg cttccgctgc ctgctgtttg ttgcgaggaa ggtcattaga gggggttaat
     3121 gctcggcgat tagcagggtc attcgggtcg cgtaacccaa gccggtcatt ggtgagggtt
     3181 tgccatgcgt taacacgagg ccggtcagcc cgatcaaagt aaggttgtag ccgttttccg
     3241 ctctgcaatt cgatgttcgg gataacaaaa ttcaattcaa gacgcccttt gtcctgatgt
     3301 tgaacccaga ggcaggcata ctggtcttta tctagaccgg tcatcaatgt ctgctcccat
     3361 tcatccatca atcgctgctt ttcgccttcg ggtaaatcac tctcctgaaa agagagcacg
     3421 ccagaggtat aagttcgggc aaattcgcag ccatcaatca gctctttgac gtgctcaggg
     3481 ttaccccgta acaccgtcgc ttgttcgcgc tgacgatcag ggcccagaag gtaatcgaca
     3541 ggaccactcc cgccaccggc accacgacca tgaatcttaa cgatcacgat gttgctccag
     3601 cagttcggca agatgttggt caatgctatt gagcaccgct aacaacgaca cccgttcttg
     3661 cggcggtaag ccatgctgat tcaagtaacg ggctatttga ttgaggttat taccgatccc
     3721 gctgacctga cgtaacaagg tcgggtctac ggtaggtagc ggacgacgcc gagctgtacg
     3781 cgattcgcct aagccaacgg ctcgtaacca ctcggccaaa tgcttacggt cacagcgttc
     3841 aagtagccgc tgatgctccg cttcggtgag tctgattttg atctctttgg tgcgcttttc
     3901 catgagaatc cgctgagaaa gtttcgcacc caaagtgcga attttcgcag tggatgcaag
     3961 gggtttcggg gggcggcgag ccccctgaaa cagtcacaga cggcacctcg aagaggggac
     4021 gctgtgtgta cTCCCTAAAT CTAGAATGCG GTcgagctcg taaacttggt ctgacagctc
     4081 tagctccggc aaaaaaacgg gcaaggtgtc accaccctgc cctttttctt taaaaccgaa
     4141 aagattactt cgcgtttgcc acctgacgtc taagaaaagg aatattcagc aatttgcccg
     4201 tgccgaagaa aggcccaccc gtgaaggtga gccagtgagt tgattgctac gtaattagtt
     4261 agttagccct tagtgactcg aattcgcggc cgcttctaga gctaaataca ttcaaatatc
     4321 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagaat
     4381 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4441 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ccgaagatca gttgggtgca
     4501 cgtgtgggtt acatcgaact ggacctcaac agcggtaaga ttcttgagag ttttcgcccc
     4561 gaagaacgtt tcccaatgat gagcactttt aaagttctgc tctgtggcgc ggtattatcc
     4621 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4681 gttgagtact caccagtcac agaaaagcat cttacggacg gcatgacagt acgcgaatta
     4741 tgcagcgctg ccataaccat gagtgataac acggcggcca acttacttct gacaacgatc
     4801 ggaggaccga aggagcttac cgcttttttg cacaacatgg gtgatcatgt aactcgcctt
     4861 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4921 cctgtagcta tggcaacaac gttgcgcaaa ctcttaactg gcgaacttct tactctcgct
     4981 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5041 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtcc
     5101 cgcggtatta ttgcagccct ggggccagat ggtaagccct cccgtatcgt agttatctac
     5161 acgacgggga gccaggcaac tatggacgaa cgtaatcgcc agatcgctga gataggtgcc
     5221 tccctgatta agcattggta ataatactag ctccggcaaa aaaacgggca aggtgtcacc
     5281 accctgccct ttttctttaa aaccgaaaag attacttcgc gtttgccacc tgacgtctaa
     5341 gaaaaggaat attcagcaat ttgcccgtgc cgaagaaagg cccacccgtg aaggtgagcc
     5401 agtgagttga ttgctacgta acaatacgca aaccgcctct cGTTCCTTAT CATCTGGCGA
     5461 ATCGGACCCA CAAGAGCACT GTTTACAGCT AGCTCAGTCC TAGGTATTAT GCTAGCtact
     5521 agagaaagag gagaaatact agatggcttc ctccgaagac gttatcaaag agttcatgcg
     5581 tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg
     5641 tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc
     5701 gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt
     5761 taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg
     5821 ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg actcctccct
     5881 gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgacgg
     5941 tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga
     6001 agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta
     6061 cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta
     6121 caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca tcgttgaaca
     6181 gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taacgctgat agtgctagtg
     6241 tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     6301 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     6361 tcgggtgggc ctttctgcgt ttatatacta gtagcggccg ctgcaggagt cactaagggt
     6421 tagttagtta gattagcaga aagtcaaaag cctccgaccg gaggcttttg actaaaactt
     6481 cccttggggt tatcattggg gctcactcaa aggcggtaat cagatgcggc gagcggtatc
     6541 agctcactca aaggcggtaa tcagataaaa aaaatcctta gctttcgcta aggatgattt
     6601 ctgctagtac gtacggtttc gaagcttgct cttcttaagc attactcgca tccattctca
     6661 ggctgtctcg tctcgtctcC TGACCTCCTG CCAGCAATAG TAAGACAACA CGCAAAGTCc
     6721 gatctcgatc ccgcgaaatt ttaatttgtt tgttagttag tttatttgtt ggtttgtttg
     6781 tgttataAAA taaGCctcta gaaataattt tgtttagctg tcaccggatg tgctttccgg
     6841 tctgatgagt ccgtgaggac gaaacagcct ctacaaattt tgtttaaaac tttaagaagg
     6901 agatatacca tgggcagcag ccatcaccat catcaccaca gccaggatcc aatggtgagc
     6961 aagggcgagg agaccacaat gggcgtaatc aagcccgaca tgaagatcaa gctgaagatg
     7021 gagggcaacg tgaatggcca cgccttcgtg atcgagggcg agggcgaggg caagccctac
     7081 gacggcacca acaccatcaa cctggaggtg aaggagggag cccccctgcc cttctcctac
     7141 gacattctga ccaccgcgtt cagttacggc aacagggcct tcaccaagta ccccgacgac
     7201 atccccaact acttcaagca gtccttcccc gagggctact cttgggagcg caccatgacc
     7261 ttcgaggaca agggcatcgt gaaggtgaag tccgacatct ccatggagga ggactccttc
     7321 atctacgaga tacacctcaa gggcgagaac ttccccccca acggccccgt gatgcagaag
     7381 gagaccaccg gctgggacgc ctccaccgag aggatgtacg tgcgcgacgg cgtgctgaag
     7441 ggcgacgtca agatgaagct gctgctggag ggcggcggcc accaccgcgt tgacttcaag
     7501 accatctaca gggccaagaa ggcggtgaag ctgcccgact atcactttgt ggaccaccgc
     7561 atcgagatcc tgaaccacga caaggactac aacaaggtga ccgtttacga gatcgccgtg
     7621 gcccgcaact ccaccgacgg catggacgag ctgtacaagg gctgaaagct tgcggccgca
     7681 taatgcttaa gtcgaacaga aagtaatcgt atgagctcga caccatcgaa tggcgcaaaa
     7741 cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtat
     7801 aagaaggaga tatacatatg cgtccggcga catacgaacc agaacagatt attgaagcag
     7861 ggctggccct gcaggctgaa ggacggaata tcaccgggtt cgcactacgt aaccaggtgg
     7921 gtggcggcaa tccgacacgt ctccgccaga tatgggacga ataccaggct tcacagagca
     7981 cggtcgtcac tgaactcgtt gccgagctgc cagtggaagt ggctgaagaa gtgaaggccg
     8041 tctccgccgc gctgtccgaa cgcatcaccc agctggcgac agaactgaat gacaaggcgg
     8101 tccgggctgc agaacgccgg gttgcggaag tcacgcgtgc tgccggtgaa cagaccgcac
     8161 aggcagagcg ggagctggcc gacgccgcgc agacagtcga cgacctggaa gaaaaactgg
     8221 ttgaactgca ggacagatat gacagtttga cgctggcgct ggagtcagaa cgttcactgc
     8281 gtcagcagca tgatgtggag atggcccagc tgaaagagcg tcttgcggcc gctgaagaga
     8341 atacccgtca gcgagaggaa cggtatcagg agcagaggac agtgctgcag gatgcgctta
     8401 atgcggagca ggcacagcac ataaacacgc gggaagacca gcagaaacga ctggagcaaa
     8461 tttctgccga agctaatgcg cgtacagaag aactgaagtc tgaacgcgat aaagtcaata
     8521 ctctccttac ccgccttgaa tcgcaggaaa atgcgctggc ctcagaacgt cagcagcatc
     8581 tggccacccg cgaaacgctg cagcaacgcc tcgagcaggc catcgctgac acgcaggcgc
     8641 gcgccggtga gattgcactt gaacgtgaca gagtcagcag cctcaccgca aggctggaat
     8701 cgcaggaaaa ggcctcctcg gagcaactgg tgcgtatggg cagtgaaata gccagtctga
     8761 cagagcgttg cacacagctg gaaaaccagc gtgatgatgc ccgtctggag acgatggggg
     8821 agaaagaaac ggtcgcggca ctgcgtggtg aggctgaagc cctgaagcgt cagaaccagt
     8881 cactgatggc ggcgctttca ggcaataaac agaccggtgg ccagaatgcg tgaggtaccc
     8941 tcgagtctgg taaagaaacc gctgctgcga aatttgaacg ccagcacatg gactcgtcta
     9001 ctagcgcagc ttaattaacc taggctgctg ccaccgctga gcaataacta gcataacccc
     9061 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaC TCGGTACCAA ATTTTCGAAA
     9121 AAAGACGCTG AAAAGCGTCT TTTTTCGTTT TGGTCCGAGC CAACTCCCTT TACAACCTCA
     9181 CTCAAGTCCG TTAGAGCCAG GATACATAGA TTACCACAAC TCCGAGCCCT TCCACCCGCC
     9241 GGCGAGACCT CTAGGCCGCA CTCGAGCACC ACCACCACCA CCACTGAGAT CCGGCTGCTA
     9301 ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC
     9361 CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG
     9421 GATAAACCTT GCTTCTTCGC GGTGgcttag tacagcatct atcgtacatc gaggtcgcat
     9481 cacgcacaaa caaaaagccc cgcaaaagca gggctgttat ctgatatagg ttgttttgtc
     9541 tcacacggca gcggaagagg aatccgaagt ggtactggta gtagtattgg atgctgctga
     9601 cgatattttc cgctttgacc caaggcttaa ataatcaatg cctgtaatca acgatctcaa
     9661 tacgccttcg gataccatag cgataaacgt atcttgctgg ttatggcttg cgatgcaaat
     9721 cgtagcatca ccttttttat actttaaaac acctgctaaa tatccatttt catctagaac
     9781 actcttaaga tgttcatttg ttattgtttg tagcgtttgc tttgtttcgc ttcgagcata
     9841 cgccttagct agcttccgag aaaaagcatc cgcatcatga ctatctttat ttactcgctc
     9901 aataaatttg cttaagtcaa caaatccctt aaaacgagtg gacatattgt taacaaaatc
     9961 agtggcagca ttttttatcc atgctttata gccaaaaaaa cgctcgaaaa cattttggtc
    10021 gtagataaat accgtatcgc cagcaaaaac aagagatgcc ttaccatcaa tagaaatcat
    10081 atcttgatct actcgagata gttctttttt gctaaaccca taaaaattat ttttctttct
    10141 tgataagttt tgaactggat attgcttctt gtatatggtt atacaattgt cgtcattatt
    10201 cttactcaaa acgaaaatga ttgagtcaac ttttgatatt agatccacac tgtcaaaatc
    10261 aacaattggg atattttcat tgccatcacc accaaaccca gcatcaaaca caatgccagt
    10321 cagaaacttc atttgctcat tatcatactg ctcagcatca tttaaaaatg aaatggtgtt
    10381 ggtgcggtca cttagtgtat taacatctga tactatcaca acccgatttt ctaaatcaga
    10441 tatagttttc tttatcacat tatcaataat ggactgtttt agctcactgt catttttaag
    10501 gatggcaatt ttatagctaa aagagtcctt agcacccgct ttacctttat ttttaaagtt
    10561 aaagtaagtg tgcaatgtaa catcgttaat atcacaatca aaatgcttat acagttctaa
    10621 aagctcttgt gctttttctt cattatgctc caaagcatca agatctaagg catcgtcact
    10681 catcatcatt cctctatgat ttttttcgcg aacgttaaat aatcatcatg atttataact
    10741 ctgataaaat cattttcttt tattaaatct ttagataaaa ctatcaactc accgtcttgc
    10801 gttttttccc ttccattagc taccacactg taagtaatct tataggcaga aaCAttaaat
    10861 aatgacaatg ttgggttgca gtgaattctt tttgttttga tgtgcaaaaa accgacgata
    10921 atcaa     
//
