LOCUS Exported 6200 bp ds-DNA circular SYN 12-DEC-2019 DEFINITION . ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6200) AUTHORS . TITLE Direct Submission JOURNAL Exported Thursday, Dec 12, 2019 from SnapGene 5.0.6 https://www.snapgene.com FEATURES Location/Qualifiers source 1..6200 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 56..222 /label=ITR /note="ITR" /note="color: #ff8000" misc_feature 239..244 /label=BglII /note="BglII" /note="color: #00ff00" misc_feature 245..716 /label=synapsin promoter /note="synapsin promoter" /note="color: #800080" CDS 748..810 /codon_start=1 /label=Ig K-Chain /note="color: #cc99ff" /translation="METDTLLLWVLLLWVPGSTGD" CDS 811..816 /codon_start=1 /label=MCS /note="MCS" /note="color: #800080" /translation="RS" CDS 817..1047 /codon_start=1 /label=X513OpuB 30-106 /note="X513OpuB 30-106" /note="color: #ff9900" /translation="ANDTVVVGSIIFTEGIIVANMVAEMIEAHTDLKVVRKLNLGGVNV NFEAIKRGGANNGIDIYVEYTGHGLVDILGFP" misc_feature 844..846 /label=K39I /note="K39I" /note="color: #ff0000; direction: BOTH" misc_feature 847..849 /label=E40I /note="E40I" /note="color: #ff0000; direction: BOTH" misc_feature 859..861 /label=Q44G /note="Q44G" /note="color: #ff0000; direction: BOTH" misc_feature 943..945 /label=E72V /note="E72V" /note="color: #ff0000; direction: BOTH" misc_feature 1018..1020 /label=T97H /note="T97H" /note="color: #ff0000; direction: BOTH" misc_feature 1042..1047 /label=ME > FP /note="color: #ff0000; direction: BOTH" CDS 1048..1323 /codon_start=1 /label=cpsfGFP 147-238 /note="cpsfGFP 149-238" /note="color: #00ff00" /translation="EPNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDG PVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK" misc_feature 1048..1053 /label=SH > EP /note="color: #ff0000; direction: BOTH" CDS 1324..1341 /codon_start=1 /label=Linker /note="Linker" /note="color: #00ff00" /translation="GGTGGS" CDS 1342..1779 /codon_start=1 /label=cpsfGFP 1-146 /note="cpsfGFP 1-146" /note="color: #00ff00" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFP" misc_feature 1777..1779 /label=N146P /note="color: #ff0000; direction: BOTH" CDS 1780..1785 /codon_start=1 /label=+PP /note="color: #ff0000" /translation="PP" CDS 1786..2379 /codon_start=1 /label=X513OpuB 107-305 /note="X513OpuB 107-305" /note="color: #ff9900" /translation="ATTDPEGAYETVKKEYKRKWNIVWLKPLGFNNTYTLTVKDELAKQ YNLKTFSDLAKISDKLILGATMFFLEGPDGYPGLQKLYNFKFKHTKSMDMGIRYTAIDN NEVQVIDAWATDGLLVSHKLKILEDDKAFFPPYYAAPIIRQDVLDKHPELKDVLNKLAN QISLEEMQKLNYKVDGEGQDPAKVAKEFLKEKGLI" misc_feature 1804..1806 /label=K113G /note="K113G" /note="color: #ff0000; direction: BOTH" misc_feature 1837..1839 /label=D124R /note="D124R" /note="color: #ff0000; direction: BOTH" misc_feature 1894..1896 /label=A143T /note="A143T" /note="color: #ff0000; direction: BOTH" misc_feature 1987..1989 /label=E174F /note="E174F" /note="color: #ff0000; direction: BOTH" misc_feature 1999..2001 /label=K178G /note="K178G" /note="color: #ff0000; direction: BOTH" misc_feature 2029..2031 /label=V188L /note="V188L" /note="color: #ff0000; direction: BOTH" misc_feature 2122..2124 /label=F219W /note="F219W" /note="color: #ff0000; direction: BOTH" misc_feature 2179..2181 /label=H238A /note="H238A" /note="color: #ff0000; direction: BOTH" misc_feature 2284..2286 /label=D273L /note="D273L" /note="color: #ff0000; direction: BOTH" CDS 2380..2391 /codon_start=1 /label=MCS /note="color: #993366" /translation="LQVD" CDS 2392..2424 /codon_start=1 /label=Myc Epitope /note="Myc Epitope" /note="color: #ff8000" /translation="EQKLISEEDLN" CDS 2425..2574 /codon_start=1 /label=PDGFR 513-561 /note="PDGFR 513-561" /note="color: #ff8000" /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ KKPR" misc_feature 2587..3175 /label=WPRE /note="WPRE" /note="color: #ff8000" misc_feature 3209..3440 /label=SV40 /note="SV40" /note="color: #ff8000" misc_feature 3469..3635 /label=ITR /note="ITR" /note="color: #ff8000" ORIGIN 1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgcgc 61 gctcgctcgc tcactgaggc cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc 121 ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat cactaggggt 181 tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg ctctaggaag 241 atctctgcag agggccctgc gtatgagtgc aagtgggttt taggaccagg atgaggcggg 301 gtgggggtgc ctacctgacg accgaccccg acccactgga caagcaccca acccccattc 361 cccaaattgc gcatccccta tcagagaggg ggaggggaaa caggatgcgg cgaggcgcgt 421 gcgcactgcc agcttcagca ccgcggacag tgccttcgcc cccgcctggc ggcgcgcgcc 481 accgccgcct cagcactgaa ggcgcgctga cgtcactcgc cggtcccccg caaactcccc 541 ttcccggcca ccttggtcgc gtccgcgccg ccgccggccc agccggaccg caccacgcga 601 ggcgcgagat aggggggcac gggcgcgacc atctgcgctg cggcgccggc gactcagcgc 661 tgcctcagtc tgcggtgggc agcggaggag tcgtgtcgtg cctgagagcg cagtcgaatt 721 caagctgcta gcaaggatcc cgccaccatg gagacagaca cactcctgct atgggtactg 781 ctgctctggg ttccaggttc cactggtgac agatctgcga acgacaccgt agttgtgggc 841 tcgatcatct ttacagaagg gattatcgtc gcaaacatgg tggcagagat gattgaggcg 901 catacagacc ttaaggtggt tcgcaaactg aaccttggcg gggtcaacgt taactttgaa 961 gccattaaac gcggaggtgc gaataatggt attgacattt acgtggagta cactgggcac 1021 ggtcttgtgg atattctggg gttcccggag ccgaacgtct atatcaccgc cgacaagcag 1081 aagaacggca tcaaggcgaa cttcaagatc cgccacaacg tggaggacgg cagcgtgcag 1141 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac 1201 aaccactacc tgagcaccca gtccgtgctg agcaaagacc ccaacgagaa gcgcgatcac 1261 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac 1321 aagggcggta ccggagggag catgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 1381 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgcg cggcgagggc 1441 gagggcgatg ccaccaacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 1501 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc 1561 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 1621 caggagcgca ccatcagctt caaggacgac ggcacctaca agacccgcgc cgaggtgaag 1681 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 1741 ggcaacatcc tggggcacaa gctggagtac aactttccgc cgcccgcgac tactgatcca 1801 gaaggtgcat acgaaaccgt gaagaaggag tacaaacgta aatggaatat tgtatggctc 1861 aaaccactgg gattcaacaa tacgtatacg cttaccgtta aagacgaact ggcgaaacag 1921 tataacctta aaaccttcag tgacttagcg aaaatctcgg ataagctgat tctgggtgca 1981 acgatgttct ttttagaagg gcccgatggt tacccaggcc tgcaaaaact gtacaatttc 2041 aaattcaagc acaccaaaag catggacatg ggtattcgct ataccgccat tgataataac 2101 gaagttcagg taattgatgc ctgggccact gatggcttgc tggtgagcca caaattaaaa 2161 attctggagg atgataaagc gttcttcccg ccgtattatg ctgcccccat catccgtcag 2221 gatgtcttag ataagcatcc tgaactgaag gacgtgctga acaaactcgc gaatcaaatt 2281 tcactggaag aaatgcagaa actgaattac aaggtggacg gtgagggtca ggacccagcg 2341 aaagtagcta aggagttttt gaaagagaaa ggtttaattc tgcaggtcga cgaacaaaaa 2401 ctcatctcag aagaggatct gaatgctgtg ggccaggaca cgcaggaggt catcgtggtg 2461 ccacactcct tgccctttaa ggtggtggtg atctcagcca tcctggccct ggtggtgctc 2521 accatcatct cccttatcat cctcatcatg ctttggcaga agaagccacg ttagaagctt 2581 atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat 2641 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct 2701 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag 2761 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc 2821 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc 2881 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct 2941 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg 3001 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg 3061 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg 3121 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga 3181 taccgtcgac ctcgacccgg gcggccgctt cgagcagaca tgataagata cattgatgag 3241 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat 3301 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc 3361 attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag caagtaaaac 3421 ctctacaaat gtggtaaaat cgataaggat cttcctagag catggctacg tagataagta 3481 gcatggcggg ttaatcatta actacaagga acccctagtg atggagttgg ccactccctc 3541 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt 3601 tgcccgggcg gcctcagtga gcgagcgagc gcgcagcctt aattaaccta attcactggc 3661 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc 3721 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc 3781 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc 3841 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 3901 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 3961 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 4021 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 4081 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 4141 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 4201 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct 4261 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 4321 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 4381 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 4441 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 4501 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 4561 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 4621 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac 4681 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 4741 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 4801 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 4861 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 4921 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 4981 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 5041 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 5101 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 5161 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 5221 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 5281 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 5341 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 5401 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 5461 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 5521 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 5581 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 5641 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 5701 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 5761 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 5821 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 5881 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 5941 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 6001 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 6061 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 6121 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 6181 gtgagcgagg aagcggaaga //