LOCUS       Exported                6443 bp ds-DNA     circular SYN 12-DEC-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Dec 12, 2019 from SnapGene 5.0.6
            https://www.snapgene.com
COMMENT     LOCUS       Exported                5933 bp ds-DNA     circular SYN 
            28-SEP-2015

            DEFINITION  synthetic circular DNA

            ACCESSION   .

            VERSION     .

            KEYWORDS    pAAV-CAG-CaMPARI-SV40pA-bGHpA

            SOURCE      synthetic DNA construct

              ORGANISM  synthetic DNA construct

            REFERENCE   1  (bases 1 to 5933)

              AUTHORS   .

              TITLE     Direct Submission

              JOURNAL   Exported Sep 28, 2015 from SnapGene 2.8.2

                        http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6443
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffff00"
     enhancer        235..538
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #800080"
     promoter        540..817
                     /label=chicken beta-actin promoter
                     /note="chicken beta-actin promoter"
                     /note="color: #00ff00"
     CDS             1148..1210
                     /codon_start=1
                     /label=Ig K-Chain
                     /note="color: #cc99ff"
                     /translation="METDTLLLWVLLLWVPGSTGD"
     CDS             1211..1216
                     /codon_start=1
                     /label=MCS
                     /note="MCS"
                     /note="color: #800080"
                     /translation="RS"
     CDS             1217..1447
                     /codon_start=1
                     /label=X513OpuB 30-106
                     /note="X513OpuB 30-106"
                     /note="color: #ff9900"
                     /translation="ANDTVVVGSIIFTEGIIVANMVAEMIEAHTDLKVVRKLNLGGVNV
                     NFEAIKRGGANNGIDIYVEYTGHGLVDILGFP"
     misc_feature    1244..1246
                     /label=K39I
                     /note="K39I"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    1247..1249
                     /label=E40I
                     /note="E40I"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    1259..1261
                     /label=Q44G
                     /note="Q44G"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    1343..1345
                     /label=E72V
                     /note="E72V"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    1418..1420
                     /label=T97H
                     /note="T97H"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    1442..1447
                     /label=ME > FP
                     /note="color: #ff0000; direction: BOTH"
     CDS             1448..1723
                     /codon_start=1
                     /label=cpsfGFP 147-238
                     /note="cpsfGFP 149-238"
                     /note="color: #00ff00"
                     /translation="EPNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDG
                     PVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     misc_feature    1448..1453
                     /label=SH > EP
                     /note="color: #ff0000; direction: BOTH"
     CDS             1724..1741
                     /codon_start=1
                     /label=Linker
                     /note="Linker"
                     /note="color: #00ff00"
                     /translation="GGTGGS"
     CDS             1742..2179
                     /codon_start=1
                     /label=cpsfGFP 1-146
                     /note="cpsfGFP 1-146"
                     /note="color: #00ff00"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFP"
     misc_feature    2177..2179
                     /label=N146P
                     /note="color: #ff0000; direction: BOTH"
     CDS             2180..2185
                     /codon_start=1
                     /label=+PP
                     /note="color: #ff0000"
                     /translation="PP"
     CDS             2186..2779
                     /codon_start=1
                     /label=X513OpuB 107-305
                     /note="X513OpuB 107-305"
                     /note="color: #ff9900"
                     /translation="ATTDPEGAYETVKKEYKRKWNIVWLKPLGFNNTYTLTVKDELAKQ
                     YNLKTFSDLAKISDKLILGATMFFLEGPDGYPGLQKLYNFKFKHTKSMDMGIRYTAIDN
                     NEVQVIDAWATDGLLVSHKLKILEDDKAFFPPYYAAPIIRQDVLDKHPELKDVLNKLAN
                     QISLEEMQKLNYKVDGEGQDPAKVAKEFLKEKGLI"
     misc_feature    2204..2206
                     /label=K113G
                     /note="K113G"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2237..2239
                     /label=D124R
                     /note="D124R"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2294..2296
                     /label=A143T
                     /note="A143T"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2387..2389
                     /label=E174F
                     /note="E174F"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2399..2401
                     /label=K178G
                     /note="K178G"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2429..2431
                     /label=V188L
                     /note="V188L"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2522..2524
                     /label=F219W
                     /note="F219W"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2579..2581
                     /label=H238A
                     /note="H238A"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2684..2686
                     /label=D273L
                     /note="D273L"
                     /note="color: #ff0000; direction: BOTH"
     CDS             2780..2791
                     /codon_start=1
                     /label=MCS
                     /note="color: #993366"
                     /translation="LQVD"
     CDS             2792..2824
                     /codon_start=1
                     /label=Myc Epitope
                     /note="Myc Epitope"
                     /note="color: #ff8000"
                     /translation="EQKLISEEDLN"
     CDS             2825..2974
                     /codon_start=1
                     /label=PDGFR 513-561
                     /note="PDGFR 513-561"
                     /note="color: #ff8000"
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     polyA_signal    2976..3110
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #8000ff"
     polyA_signal    3202..3409
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #8000ff"
     repeat_region   3439..3579
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffff00"
     rep_origin      3675..4130
                     /direction=RIGHT
                     /label=F1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #c0c0c0"
     promoter        4462..4566
                     /label=bla
                     /note="AmpR promoter"
                     /note="color: #8080ff"
     CDS             4567..5427
                     /codon_start=1
                     /label=bla
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #0000ff"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5598..6186
                     /direction=RIGHT
                     /label=pUC Ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #c0c0c0"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtagatc tgaattcggt accctagtta
      181 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      241 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      301 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      361 ggactattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      421 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      481 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      541 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat
      601 tttgtattta tttatttttt aattattttg tgcagcgatg ggggcggggg gggggggggg
      661 gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc
      721 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg
      781 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcga cgctgccttc
      841 gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
      901 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
      961 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagc
     1021 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt
     1081 gctggttatt gtgctgtctc atcattttgg caaagaattc ctcgaagatc cgaaggggtt
     1141 caagcttatg gagacagaca cactcctgct atgggtactg ctgctctggg ttccaggttc
     1201 cactggtgac agatctgcga acgacaccgt agttgtgggc tcgatcatct ttacagaagg
     1261 gattatcgtc gcaaacatgg tggcagagat gattgaggcg catacagacc ttaaggtggt
     1321 tcgcaaactg aaccttggcg gggtcaacgt taactttgaa gccattaaac gcggaggtgc
     1381 gaataatggt attgacattt acgtggagta cactgggcac ggtcttgtgg atattctggg
     1441 gttcccggag ccgaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggcgaa
     1501 cttcaagatc cgccacaacg tggaggacgg cagcgtgcag ctcgccgacc actaccagca
     1561 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     1621 gtccgtgctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     1681 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggcggta ccggagggag
     1741 catgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1801 cgacgtaaac ggccacaagt tcagcgtgcg cggcgagggc gagggcgatg ccaccaacgg
     1861 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1921 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     1981 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcagctt
     2041 caaggacgac ggcacctaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     2101 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     2161 gctggagtac aactttccgc cgcccgcgac tactgatcca gaaggtgcat acgaaaccgt
     2221 gaagaaggag tacaaacgta aatggaatat tgtatggctc aaaccactgg gattcaacaa
     2281 tacgtatacg cttaccgtta aagacgaact ggcgaaacag tataacctta aaaccttcag
     2341 tgacttagcg aaaatctcgg ataagctgat tctgggtgca acgatgttct ttttagaagg
     2401 gcccgatggt tacccaggcc tgcaaaaact gtacaatttc aaattcaagc acaccaaaag
     2461 catggacatg ggtattcgct ataccgccat tgataataac gaagttcagg taattgatgc
     2521 ctgggccact gatggcttgc tggtgagcca caaattaaaa attctggagg atgataaagc
     2581 gttcttcccg ccgtattatg ctgcccccat catccgtcag gatgtcttag ataagcatcc
     2641 tgaactgaag gacgtgctga acaaactcgc gaatcaaatt tcactggaag aaatgcagaa
     2701 actgaattac aaggtggacg gtgagggtca ggacccagcg aaagtagcta aggagttttt
     2761 gaaagagaaa ggtttaattc tgcaggtcga cgaacaaaaa ctcatctcag aagaggatct
     2821 gaatgctgtg ggccaggaca cgcaggaggt catcgtggtg ccacactcct tgccctttaa
     2881 ggtggtggtg atctcagcca tcctggccct ggtggtgctc accatcatct cccttatcat
     2941 cctcatcatg ctttggcaga agaagccacg ttagggatcc agacatgata agatacattg
     3001 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     3061 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     3121 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt tagtcgacta
     3181 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     3241 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     3301 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     3361 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggag agatctcacg
     3421 tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg
     3481 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
     3541 cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg ctgcagccag
     3601 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gtagcctgaa
     3661 tggcgaatgg cgcgacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     3721 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     3781 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     3841 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     3901 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     3961 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     4021 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4081 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt cctgatgcgg
     4141 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
     4201 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     4261 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     4321 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     4381 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     4441 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4501 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4561 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4621 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4681 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4741 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     4801 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     4861 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4921 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     4981 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5041 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5101 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5161 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5221 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5281 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5341 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5401 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5461 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5521 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5581 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5641 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     5701 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     5761 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5821 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5881 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5941 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
     6001 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6061 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6121 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6181 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6241 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6301 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6361 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     6421 agctgggctg caggctcaca tgt
//
