LOCUS       Exported                6885 bp ds-DNA     circular SYN 12-DEC-2019
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Dec 12, 2019 from SnapGene 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6885
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(57..223)
                     /label=ITR
                     /note="ITR"
                     /note="color: #ff6666; direction: LEFT"
     misc_feature    complement(251..483)
                     /label=SV40
                     /note="SV40"
                     /note="color: #cccccc; direction: LEFT"
     misc_feature    complement(511..1099)
                     /label=WPRE
                     /note="WPRE"
                     /note="color: #50ff50; direction: LEFT"
     misc_feature    678..698
                     /label=wpre pcr rev
                     /note="wpre pcr rev"
                     /note="color: #00e600; direction: RIGHT"
     misc_feature    complement(1133..1166)
                     /label=loxP
                     /note="loxP"
                     /note="color: #ff0000; direction: LEFT"
     CDS             1299..1361
                     /codon_start=1
                     /label=Ig K-Chain
                     /note="color: #cc99ff"
                     /translation="METDTLLLWVLLLWVPGSTGD"
     CDS             1362..1367
                     /codon_start=1
                     /label=MCS
                     /note="MCS"
                     /note="color: #800080"
                     /translation="RS"
     CDS             1368..1598
                     /codon_start=1
                     /label=X513OpuB 30-106
                     /note="X513OpuB 30-106"
                     /note="color: #ff9900"
                     /translation="ANDTVVVGSIIFTEGIIVANMVAEMIEAHTDLKVVRKLNLGGVNV
                     NFEAIKRGGANNGIDIYVEYTGHGLVDILGFP"
     misc_feature    1395..1397
                     /label=K39I
                     /note="K39I"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    1398..1400
                     /label=E40I
                     /note="E40I"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    1410..1412
                     /label=Q44G
                     /note="Q44G"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    1494..1496
                     /label=E72V
                     /note="E72V"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    1569..1571
                     /label=T97H
                     /note="T97H"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    1593..1598
                     /label=ME > FP
                     /note="color: #ff0000; direction: BOTH"
     CDS             1599..1874
                     /codon_start=1
                     /label=cpSFVenus 147-238
                     /note="cpGFP 147-238"
                     /note="color: #ffff00"
                     /translation="EPNVYITADKQKNGIKANFKIRHNVEDGSVQLADHYQQNTPIGDG
                     PVLLPDNHYLSYQSVLSKDPNEKRDHMVLLEFVTAAGITLGMNELYK"
     misc_feature    1599..1604
                     /label=SH>EP
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    1617..1619
                     /label=M153T (sfGFP)
                     /note="M153T (GFPuv > sfGFP)"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    1632..1634
                     /label=R158K
                     /note="R158K (some sensors it is R, others it is K, 
                     seemingly meaningless)"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    1647..1649
                     /label=V163A (sfGFP)
                     /note="V163A (sfGFP)"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    1671..1673
                     /label=I171V (sfGFP)
                     /note="I171V (sfGFP)"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    1683..1685
                     /label=G175S
                     /note="G175S (175S in sfGFP, 175G in Venus. Keeping 175S.) 
                     "
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    1698..1700
                     /label=Y180D - spontaneous. Surface mutation
                     /note="Y180D - spontaneous. Surface mutation, not expected 
                     to affect Fluorescence."
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    1767..1769
                     /label=T203Y (Major color changing in Venus)
                     /note="T203Y (Major color changing in Venus)"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    1776..1778
                     /label=A206V (sfGFP)
                     /note="A206V (sfGFP)"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    1782..1784
                     /label=Silent codon change
                     /note="Silent codon change"
                     /note="color: #ff0000; direction: BOTH"
     CDS             1875..1892
                     /codon_start=1
                     /label=Linker
                     /note="Linker"
                     /note="color: #ffff00"
                     /translation="GGTGGS"
     CDS             1893..2330
                     /codon_start=1
                     /label=cpSFVenus 1-146
                     /note="cpGFP 1-146"
                     /note="color: #ffff00"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFP"
     misc_feature    1980..1982
                     /label=S30R (sfGFP)
                     /note="S30R (sfGFP)"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2007..2009
                     /label=Y39N (sfGFP)
                     /note="Y39N (sfGFP)"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2028..2030
                     /label=F46L (Venus fast maturation)
                     /note="F46L (Venus fast maturation)"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2085..2087
                     /label=T65G (Venus chromophore)
                     /note="T65G (Venus chromophore)"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2094..2096
                     /label=V68L in Venus. (Keeping V from sfGFP)
                     /note="V68L in Venus. Keeping V from sfGFP)"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2106..2108
                     /label=S72A (Venus)
                     /note="S72A (Venus)"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2169..2171
                     /label=I93V (sfGFP)
                     /note="I93V (sfGFP)"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2187..2189
                     /label=F99S (sfGFP)
                     /note="F99S (sfGFP)"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2205..2207
                     /label=N105T (sfGFP)
                     /note="N105T (sfGFP)"
                     /note="color: #ff0000; direction: BOTH"
     CDS             2331..2336
                     /codon_start=1
                     /label=+PP
                     /note="color: #ff0000"
                     /translation="PP"
     CDS             2337..2930
                     /codon_start=1
                     /label=X513OpuB 107-305
                     /note="X513OpuB 107-305"
                     /note="color: #ff9900"
                     /translation="ATTDPEGAYETVKKEYKRKWNIVWLKPLGFNNTATLTVKDELAKQ
                     YNLKTFSDLAKISDKLILGATMFFLEGPDGYPGLQKLYNFKFKHTKSMDMGIRYTAIDN
                     NEVQVIDAWATDGLLVSHKLKILEDDKAFFPPYYAAPIIRQDVLDKHPELKDVLNKLAN
                     QISLEEMQKLNYKVDGEGQDPAKVAKEFLKEKGLI"
     misc_feature    2355..2357
                     /label=K113G
                     /note="K113G"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2388..2390
                     /label=D124R
                     /note="D124R"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2436..2438
                     /label=Y140A (NULL)
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2445..2447
                     /label=A143T
                     /note="A143T"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2538..2540
                     /label=E174F
                     /note="E174F"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2550..2552
                     /label=K178G
                     /note="K178G"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2580..2582
                     /label=V188L
                     /note="V188L"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2673..2675
                     /label=F219W
                     /note="F219W"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2730..2732
                     /label=H238A
                     /note="H238A"
                     /note="color: #ff0000; direction: BOTH"
     misc_feature    2835..2837
                     /label=D273L
                     /note="D273L"
                     /note="color: #ff0000; direction: BOTH"
     CDS             2931..2942
                     /codon_start=1
                     /label=MCS
                     /note="color: #993366"
                     /translation="LQVD"
     CDS             2943..2975
                     /codon_start=1
                     /label=Myc Epitope
                     /note="Myc Epitope"
                     /note="color: #ff8000"
                     /translation="EQKLISEEDLN"
     CDS             2976..3125
                     /codon_start=1
                     /label=PDGFR 513-561
                     /note="PDGFR 513-561"
                     /note="color: #ff8000"
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     misc_feature    complement(3153..3156)
                     /label=AAV21_Syn_FLEX_WPRE-MCS-iGluSnFr
                     /note="color: #ff0000; direction: LEFT"
     misc_feature    complement(3153..3156)
                     /label=MCS-iGluSnFr reverse&#x1E;&#x1F;
                     /note="color: #ff6666; direction: LEFT"
     misc_feature    complement(3158..3191)
                     /label=loxP
                     /note="loxP"
                     /note="color: #ff8000; direction: LEFT"
     misc_feature    3254..3287
                     /label=loxP2272
                     /note="loxP2272"
                     /note="color: #ff0000; direction: RIGHT"
     promoter        complement(3298..4233)
                     /label=CBA promoter
                     /note="CBA promoter"
                     /note="color: #50ff50; direction: LEFT"
     misc_feature    complement(3407..3427)
                     /label=CAG pcr for
                     /note="CAG pcr for"
                     /note="color: #ff8000; direction: LEFT"
     misc_feature    complement(4260..4427)
                     /label=ITR
                     /note="ITR"
                     /note="color: #00ffff; direction: LEFT"
     primer_bind     complement(4482..4501)
                     /label=Untitled
                     /note="color: #008000; direction: LEFT"
     misc_feature    5085..5942
                     /label=Amp Resistance
                     /note="Amp Resistance"
                     /note="color: #ff8000"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgcg
       61 cgctcgctcg ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg
      121 cccggcctca gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg
      181 ttccttgtag ttaatgatta acccgccatg ctacttatct acgtagccat gctctaggaa
      241 gatccttatc gattttacca catttgtaga ggttttactt gctttaaaaa acctcccaca
      301 tctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
      361 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
      421 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgctc
      481 gaagcggccg cccgggtcga cggtatcgat gcggggaggc ggcccaaagg gagatccgac
      541 tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg
      601 aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa
      661 tccaggtggc aacacaggcg agcagccaag gaaaggacga tgatttcccc gacaacacca
      721 cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga
      781 tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg
      841 tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac
      901 gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg
      961 aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt
     1021 atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt
     1081 tgtaatccag aggttgatta tcgataagct aattcgaacc tcttcgaggg acataacttc
     1141 gtataatgta tgctatacga agttatgggt cgatggtgag atctggacta gagggtcgat
     1201 ggtgatgctt ggatcaaaat aggaagaatc ccaatgataa cttcgtataa agtatcctat
     1261 acgaagttat ggatccgaat tcactagtct cgagcaaaat ggagacagac acactcctgc
     1321 tatgggtact gctgctctgg gttccaggtt ccactggtga cagatctgcg aacgacaccg
     1381 tagttgtggg ctcgatcatc tttacagaag ggattatcgt cgcaaacatg gtggcagaga
     1441 tgattgaggc gcatacagac cttaaggtgg ttcgcaaact gaaccttggc ggggtcaacg
     1501 ttaactttga agccattaaa cgcggaggtg cgaataatgg tattgacatt tacgtggagt
     1561 acactgggca cggtcttgtg gatattctgg ggttcccgga gccgaacgtc tatatcaccg
     1621 ccgacaagca gaagaacggc atcaaggcga acttcaagat ccgccacaac gtggaggacg
     1681 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     1741 tgctgcccga caaccactac ctgagctatc agtccgtgct gagcaaagac cccaacgaga
     1801 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatga
     1861 acgagctgta caagggcggt accggaggga gcatgagcaa gggcgaggag ctgttcaccg
     1921 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgc
     1981 gcggcgaggg cgagggcgat gccaccaacg gcaagctgac cctgaagctc ctgtgcacca
     2041 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcgtgcagt
     2101 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     2161 aaggctacgt ccaggagcgc accatcagct tcaaggacga cggcacctac aagacccgcg
     2221 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     2281 tcaaggagga cggcaacatc ctggggcaca agctggagta caactttccg ccgcccgcga
     2341 ctactgatcc agaaggtgca tacgaaaccg tgaagaagga gtacaaacgt aaatggaata
     2401 ttgtatggct caaaccactg ggattcaaca atacggcgac gcttaccgtt aaagacgaac
     2461 tggcgaaaca gtataacctt aaaaccttca gtgacttagc gaaaatctcg gataagctga
     2521 ttctgggtgc aacgatgttc tttttagaag ggcccgatgg ttacccaggc ctgcaaaaac
     2581 tgtacaattt caaattcaag cacaccaaaa gcatggacat gggtattcgc tataccgcca
     2641 ttgataataa cgaagttcag gtaattgatg cctgggccac tgatggcttg ctggtgagcc
     2701 acaaattaaa aattctggag gatgataaag cgttcttccc gccgtattat gctgccccca
     2761 tcatccgtca ggatgtctta gataagcatc ctgaactgaa ggacgtgctg aacaaactcg
     2821 cgaatcaaat ttcactggaa gaaatgcaga aactgaatta caaggtggac ggtgagggtc
     2881 aggacccagc gaaagtagct aaggagtttt tgaaagagaa aggtttaatt ctgcaggtcg
     2941 acgaacaaaa actcatctca gaagaggatc tgaatgctgt gggccaggac acgcaggagg
     3001 tcatcgtggt gccacactcc ttgcccttta aggtggtggt gatctcagcc atcctggccc
     3061 tggtggtgct caccatcatc tcccttatca tcctcatcat gctttggcag aagaagccac
     3121 gttaggatat caccggtggc gcgccaagct tgctagtata acttcgtata gcatacatta
     3181 tacgaagtta tgggtcgatg gtgatgcttg gcaattcggg tcgatggtga agcattggtc
     3241 ttcctatttt gatataactt cgtataggat actttatacg aagttatggg gatgatccaa
     3301 ttctttgcca aaatgatgag acagcacaat aaccagcacg ttgcccagga gctgtaggaa
     3361 aaagaagaag gcatgaacat ggttagcaga ggctctagag ccgccggtca cacgccagaa
     3421 gccgaacccc gccctgcccc gtcccccccg aaggcagccg tccccccgcg gacagccccg
     3481 agccgctccc cccgcacaaa gggccctccc ggagcccctc aaggctttca cgcagccaca
     3541 gaaaagaaac aagccgtcat taaaccaagc gctaattaca gcccggagga gaagggccgt
     3601 cccgcccgct cacctgtggg agtaacgcgg tcagtcagag ccggggcggg cggcgcgagg
     3661 cggcggcgga gcggggcacg gggcgaaggc agcgtcgcag cgactcccgc ccgccgcgcg
     3721 cttcgctttt tatagggccg ccgccgccgc cgcctcgcca taaaaggaaa ctttcggagc
     3781 gcgccgctct gattggctgc cgccgcacct ctccgcctcg ccccgccccg cccctcgccc
     3841 cgccccgccc cgcctggcgc gcgccccccc cccccccccg cccccatcgc tgcacaaaat
     3901 aattaaaaaa taaataaata caaaattggg ggtggggagg ggggggagat ggggagagtg
     3961 aagcagaacg tggggctcac ctcgaccatg gtaatagcga tgactaatac gtagatgtac
     4021 tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta
     4081 ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag
     4141 tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt
     4201 actatgggaa catacgtcat tattgacgtc aatggtacga tcttcctaga gcatggctac
     4261 gtagataagt agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg
     4321 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     4381 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg gcgtaatagc
     4441 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     4501 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     4561 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     4621 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4681 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4741 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4801 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4861 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4921 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     4981 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5041 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5101 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5161 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5221 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5281 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5341 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5401 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5461 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5521 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5581 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     5641 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     5701 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     5761 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     5821 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     5881 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     5941 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6001 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6061 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6121 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6181 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6241 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     6301 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6361 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6421 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6481 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6541 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6601 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6661 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6721 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6781 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     6841 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaag
//
