LOCUS       pFd029.R2               3203 bp ds-DNA     circular     24-JUL-2019
DEFINITION  .
KEYWORDS    "Pcpcg2" "ccaR" "marker:Chloramphenicol" "origin:ColE1" "sfGFP"    
FEATURES             Location/Qualifiers
     Promoter region 1201..1219
                     /label="O2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Terminator      2466..2570
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     RBS             1805..1838
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#7bcdc8
                     /ApEinfo_fwdcolor=#7bcdc8
     Promoter region 1230..1230
                     /label="+1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1770..1775
                     /label="-35"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        1176..1249
                     /label="PLtetO-1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     rep_origin      complement(join(2703..3203,1..182))
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     CDS             complement(309..968)
                     /label="CmR"
                     /ApEinfo_revcolor=#fff79a
                     /ApEinfo_fwdcolor=#fff79a
     misc_feature    1596..1724
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     misc_feature    1793..1798
                     /label="-10"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     Promoter        1770..1804
                     /label="J23106"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     Promoter region 1218..1223
                     /label="-10"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             1839..2465
                     /label="tetR"
                     /ApEinfo_revcolor=#8493ca
                     /ApEinfo_fwdcolor=#8493ca
     misc_feature    1805..1805
                     /label="+1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1572..1575
                     /label="GG overlap"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1275..1571
                     /label="pmN1Fd1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     terminator      187..292
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    1261..1264
                     /label="GG overlap"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1250..1274
                     /label="sRBS (7952.44 AU)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     Promoter region 1176..1194
                     /label="O2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Promoter region 1195..1200
                     /label="-35"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
       61 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
      121 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT
      181 GACTAGTGCT TGGATTCTCA CCAATAAAAA ACGCCCGGCG GCAACCGAGC GTTCTGAACA
      241 AATCCAGATG GAGTTCTGAG GTCATTACTG GATCTATCAA CAGGAGTCCA AGCGAGCTCG
      301 ATATCAAATT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT
      361 CTGCCGACAT GGAAGCCATC ACAGACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC
      421 ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC
      481 ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC TGAGACGAAA
      541 AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA
      601 TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT
      661 GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC
      721 ACCAGCTCAC CGTCTTTCAT TGCCATACGA AATTCCGGAT GAGCATTCAT CAGGCGGGCA
      781 AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT CTTTAAAAAG
      841 GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA CTGAAATGCC
      901 TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC AGTGATTTTT
      961 TTCTCCATTT TAGCTTCCTT AGCTCCTGAA AATCTCGATA ACTCAAAAAA TACGCCCGGT
     1021 AGTGATCTTA TTTCATTATG GTGAAAGTTG GAACCTCTTA CGTGCCGATC AACGTCTCAT
     1081 TTTCGCCAGA TATCGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA
     1141 GGCGTATCAC GAGGCCCTTT CGTCTTCACC TCGAGTCCCT ATCAGTGATA GAGATTGACA
     1201 TCCCTATCAG TGATAGAGAT ACTGAGCACA TCAGCAGGAC GCACTGACCG ATTACCGGCA
     1261 ACAGGAGACA CAAAATGACC TCCTTCAAAA TCACCTTCAT CAACGAATTG AGCGGTCTGA
     1321 AAGAAATTAT TGATATCCCT GATGATAAAT ACATTCTGGA CGCCGCATGC GAACAAAACA
     1381 TCGAACTGCC TGCATCCTGC CGGGAAGGCA CTTGCTCGAC CTGCGTGGCA AAGTTGGAAA
     1441 AAGGGAACGT AGACCAACAG GACCAGAGCT TCTTGGATGA CGACCAGATT GAAAAGGGTT
     1501 ACATTCTGAT CTGTAAAGCG TACCCACTGT CAGATTGTAT TATTCGGACT CATGCGGAAG
     1561 ACCAGTTATA TTAAgaattc TGATAATAAT CTAGACCAGG CATCAAATAA AACGAAAGGC
     1621 TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCTACTA
     1681 GAGTCACACT GGCTCACCTT CGGGTGGGCC TTTCTGCGTT TATAGGTACC CGTGGATCCT
     1741 CTAGAGTCGA CCTGCAGGCA TGCAAGCTTT TTACGGCTAG CTCAGTCCTA GGTATAGTGC
     1801 TAGCCCAGCC AGAGAAACAC TCTTTAACAG GGGGTAGTAT GATGTCTCGT TTAGATAAAA
     1861 GTAAAGTGAT TAACAGCGCA TTAGAGCTGC TTAATGAGGT CGGAATCGAA GGTTTAACAA
     1921 CCCGTAAACT CGCCCAGAAG CTAGGTGTAG AGCAGCCTAC ATTGTATTGG CATGTAAAAA
     1981 ATAAGCGGGC TTTGCTCGAC GCCTTAGCCA TTGAGATGTT AGATAGGCAC CATACTCACT
     2041 TTTGCCCTTT AGAAGGGGAA AGCTGGCAAG ATTTTTTACG TAATAACGCT AAAAGTTTTA
     2101 GATGTGCTTT ACTAAGTCAT CGCGATGGAG CAAAAGTACA TTTAGGTACA CGGCCTACAG
     2161 AAAAACAGTA TGAAACTCTC GAAAATCAAT TAGCCTTTTT ATGCCAACAA GGTTTTTCAC
     2221 TAGAGAATGC ATTATATGCA CTCAGCGCAG TGGGGCATTT TACTTTAGGT TGCGTATTGG
     2281 AAGATCAAGA GCATCAAGTC GCTAAAGAAG AAAGGGAAAC ACCTACTACT GATAGTATGC
     2341 CGCCATTATT ACGACAAGCT ATCGAATTAT TTGATCACCA AGGTGCAGAG CCAGCCTTCT
     2401 TATTCGGCCT TGAATTGATC ATATGCGGAT TAGAAAAACA ACTTAAATGT GAAAGTGGGT
     2461 CTTAAGGCAT CAAATAAAAC GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC GTTTTATCTG
     2521 TTGTTTGTCG GTGAACGCTC TCCTGAGTAG GACAAATCCG CCGCCCTAGA CCTAGGCGTT
     2581 CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA
     2641 GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA
     2701 AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT
     2761 CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC
     2821 CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC
     2881 GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCAATGCT CACGCTGTAG GTATCTCAGT
     2941 TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC
     3001 CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG
     3061 CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA
     3121 GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT TGGTATCTGC
     3181 GCTCTGCTGA AGCCAGTTAC CTT
//