LOCUS       pFd031                  3203 bp ds-DNA     circular     31-MAY-2019
DEFINITION  .
KEYWORDS    "Pcpcg2" "ccaR" "marker:Chloramphenicol" "origin:ColE1" "sfGFP"    
FEATURES             Location/Qualifiers
     promoter        109..182
                     /label="PLtetO-1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     terminator      2323..2428
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    738..738
                     /label="+1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    204..207
                     /label="GG overlap"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    726..731
                     /label="-10"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     rep_origin      complement(1636..2318)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     Promoter region 109..127
                     /label="O2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             772..1398
                     /label="tetR"
                     /ApEinfo_revcolor=#8493ca
                     /ApEinfo_fwdcolor=#8493ca
     CDS             complement(2445..3104)
                     /label="CmR"
                     /ApEinfo_revcolor=#fff79a
                     /ApEinfo_fwdcolor=#fff79a
     Promoter region 128..133
                     /label="-35"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    183..207
                     /label="sRBS (2707.62)"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Promoter region 134..152
                     /label="O2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    703..708
                     /label="-35"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    208..504
                     /label="pm9211Fd1"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    505..508
                     /label="GG overlap"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     RBS             738..771
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#7bcdc8
                     /ApEinfo_fwdcolor=#7bcdc8
     misc_feature    529..657
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     Promoter region 163..163
                     /label="+1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     Terminator      1399..1503
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     Promoter region 151..156
                     /label="-10"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     Promoter        703..737
                     /label="J23106"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
ORIGIN
        1 ATCAACGTCT CATTTTCGCC AGATATCGAC GTCTAAGAAA CCATTATTAT CATGACATTA
       61 ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTTC ACCTCGAGTC CCTATCAGTG
      121 ATAGAGATTG ACATCCCTAT CAGTGATAGA GATACTGAGC ACATCAGCAG GACGCACTGA
      181 CCTGCGTCTG TTACGGGAAA TTTAAGAATG GCTTCGTACA AGGTAACTTT GGTGAACGCG
      241 AACGAGGGCT TAAACTCTAC TATTGAAGTG CCTGATGATC AATATATCCT CGACGCCGCT
      301 GAGGAACAAG GCATTGACCT TCCTTACTCT TGCCGCGCAG GCGCATGCTC TACTTGCGCC
      361 GGGAAGATCA CGGCGGGTTC AGTCGACCAG TCAGATCAAA GCTTTCTCGA CGACGATCAG
      421 CTCGAGGCGG GGTTCGTCCT TACCTGCGTC GCGTATCCTA CAAGCGATGT CACGATTACT
      481 ACTCATGCGG AGGAGGAGTT ATACTAAgaa ttcTGATAAT AATCTAGACC AGGCATCAAA
      541 TAAAACGAAA GGCTCAGTCG AAAGACTGGG CCTTTCGTTT TATCTGTTGT TTGTCGGTGA
      601 ACGCTCTCTA CTAGAGTCAC ACTGGCTCAC CTTCGGGTGG GCCTTTCTGC GTTTATAGGT
      661 ACCCGTGGAT CCTCTAGAGT CGACCTGCAG GCATGCAAGC TTTTTACGGC TAGCTCAGTC
      721 CTAGGTATAG TGCTAGCCCA GCCAGAGAAA CACTCTTTAA CAGGGGGTAG TATGATGTCT
      781 CGTTTAGATA AAAGTAAAGT GATTAACAGC GCATTAGAGC TGCTTAATGA GGTCGGAATC
      841 GAAGGTTTAA CAACCCGTAA ACTCGCCCAG AAGCTAGGTG TAGAGCAGCC TACATTGTAT
      901 TGGCATGTAA AAAATAAGCG GGCTTTGCTC GACGCCTTAG CCATTGAGAT GTTAGATAGG
      961 CACCATACTC ACTTTTGCCC TTTAGAAGGG GAAAGCTGGC AAGATTTTTT ACGTAATAAC
     1021 GCTAAAAGTT TTAGATGTGC TTTACTAAGT CATCGCGATG GAGCAAAAGT ACATTTAGGT
     1081 ACACGGCCTA CAGAAAAACA GTATGAAACT CTCGAAAATC AATTAGCCTT TTTATGCCAA
     1141 CAAGGTTTTT CACTAGAGAA TGCATTATAT GCACTCAGCG CAGTGGGGCA TTTTACTTTA
     1201 GGTTGCGTAT TGGAAGATCA AGAGCATCAA GTCGCTAAAG AAGAAAGGGA AACACCTACT
     1261 ACTGATAGTA TGCCGCCATT ATTACGACAA GCTATCGAAT TATTTGATCA CCAAGGTGCA
     1321 GAGCCAGCCT TCTTATTCGG CCTTGAATTG ATCATATGCG GATTAGAAAA ACAACTTAAA
     1381 TGTGAAAGTG GGTCTTAAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT
     1441 TTCGTTTTAT CTGTTGTTTG TCGGTGAACG CTCTCCTGAG TAGGACAAAT CCGCCGCCCT
     1501 AGACCTAGGC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT
     1561 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC
     1621 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA
     1681 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA
     1741 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC
     1801 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT GCTCACGCTG
     1861 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC
     1921 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG
     1981 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT
     2041 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT
     2101 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG
     2161 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC
     2221 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA
     2281 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGACT AGTGCTTGGA TTCTCACCAA
     2341 TAAAAAACGC CCGGCGGCAA CCGAGCGTTC TGAACAAATC CAGATGGAGT TCTGAGGTCA
     2401 TTACTGGATC TATCAACAGG AGTCCAAGCG AGCTCGATAT CAAATTACGC CCCGCCCTGC
     2461 CACTCATCGC AGTACTGTTG TAATTCATTA AGCATTCTGC CGACATGGAA GCCATCACAG
     2521 ACGGCATGAT GAACCTGAAT CGCCAGCGGC ATCAGCACCT TGTCGCCTTG CGTATAATAT
     2581 TTGCCCATGG TGAAAACGGG GGCGAAGAAG TTGTCCATAT TGGCCACGTT TAAATCAAAA
     2641 CTGGTGAAAC TCACCCAGGG ATTGGCTGAG ACGAAAAACA TATTCTCAAT AAACCCTTTA
     2701 GGGAAATAGG CCAGGTTTTC ACCGTAACAC GCCACATCTT GCGAATATAT GTGTAGAAAC
     2761 TGCCGGAAAT CGTCGTGGTA TTCACTCCAG AGCGATGAAA ACGTTTCAGT TTGCTCATGG
     2821 AAAACGGTGT AACAAGGGTG AACACTATCC CATATCACCA GCTCACCGTC TTTCATTGCC
     2881 ATACGAAATT CCGGATGAGC ATTCATCAGG CGGGCAAGAA TGTGAATAAA GGCCGGATAA
     2941 AACTTGTGCT TATTTTTCTT TACGGTCTTT AAAAAGGCCG TAATATCCAG CTGAACGGTC
     3001 TGGTTATAGG TACATTGAGC AACTGACTGA AATGCCTCAA AATGTTCTTT ACGATGCCAT
     3061 TGGGATATAT CAACGGTGGT ATATCCAGTG ATTTTTTTCT CCATTTTAGC TTCCTTAGCT
     3121 CCTGAAAATC TCGATAACTC AAAAAATACG CCCGGTAGTG ATCTTATTTC ATTATGGTGA
     3181 AAGTTGGAAC CTCTTACGTG CCG
//