LOCUS       pFd029.R2.RFP           3911 bp ds-DNA     circular     12-DEC-2019
DEFINITION  .
KEYWORDS    "Pcpcg2" "ccaR" "marker:Chloramphenicol" "origin:ColE1" "sfGFP"    
FEATURES             Location/Qualifiers
     RBS             2513..2546
                     /label="sRBS (50,000)"
                     /ApEinfo_revcolor=#7bcdc8
                     /ApEinfo_fwdcolor=#7bcdc8
     misc_feature    2280..2283
                     /label="GG overlap"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     Promoter region 1218..1223
                     /label="-10"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1250..1274
                     /label="sRBS (7952.44 AU)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             2547..3173
                     /label="tetR"
                     /ApEinfo_revcolor=#8493ca
                     /ApEinfo_fwdcolor=#8493ca
     misc_feature    1572..1607
                     /label="LINKER-12"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     Promoter region 1195..1200
                     /label="-35"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     Promoter region 1230..1230
                     /label="+1"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     Promoter region 1176..1194
                     /label="O2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1572..1575
                     /label="GG cut site"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     terminator      187..292
                     /label="T0"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     misc_feature    1261..1264
                     /label="GG overlap"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2501..2506
                     /label="-10"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        1176..1249
                     /label="PLtetO-1"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1275..1571
                     /label="pmN1Fd1"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     Promoter        2478..2512
                     /label="J23106"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2513..2513
                     /label="+1"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2304..2432
                     /label="Terminator B0015"
                     /ApEinfo_revcolor=#ff0000
                     /ApEinfo_fwdcolor=#ff0000
     CDS             complement(309..968)
                     /label="CmR"
                     /ApEinfo_revcolor=#fff79a
                     /ApEinfo_fwdcolor=#fff79a
     rep_origin      complement(join(3411..3911,1..182))
                     /label="ColE1 origin"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     misc_feature    2478..2483
                     /label="-35"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             1608..2282
                     /label="RFP"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     Terminator      3174..3278
                     /label="T1"
                     /ApEinfo_revcolor=#ff0517
                     /ApEinfo_fwdcolor=#ff0517
     Promoter region 1201..1219
                     /label="O2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT
       61 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT
      121 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT
      181 GACTAGTGCT TGGATTCTCA CCAATAAAAA ACGCCCGGCG GCAACCGAGC GTTCTGAACA
      241 AATCCAGATG GAGTTCTGAG GTCATTACTG GATCTATCAA CAGGAGTCCA AGCGAGCTCG
      301 ATATCAAATT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT
      361 CTGCCGACAT GGAAGCCATC ACAGACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC
      421 ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC
      481 ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC TGAGACGAAA
      541 AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA
      601 TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT
      661 GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC
      721 ACCAGCTCAC CGTCTTTCAT TGCCATACGA AATTCCGGAT GAGCATTCAT CAGGCGGGCA
      781 AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT CTTTAAAAAG
      841 GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA CTGAAATGCC
      901 TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC AGTGATTTTT
      961 TTCTCCATTT TAGCTTCCTT AGCTCCTGAA AATCTCGATA ACTCAAAAAA TACGCCCGGT
     1021 AGTGATCTTA TTTCATTATG GTGAAAGTTG GAACCTCTTA CGTGCCGATC AACGTCTCAT
     1081 TTTCGCCAGA TATCGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA
     1141 GGCGTATCAC GAGGCCCTTT CGTCTTCACC TCGAGTCCCT ATCAGTGATA GAGATTGACA
     1201 TCCCTATCAG TGATAGAGAT ACTGAGCACA TCAGCAGGAC GCACTGACCG ATTACCGGCA
     1261 ACAGGAGACA CAAAATGACC TCCTTCAAAA TCACCTTCAT CAACGAATTG AGCGGTCTGA
     1321 AAGAAATTAT TGATATCCCT GATGATAAAT ACATTCTGGA CGCCGCATGC GAACAAAACA
     1381 TCGAACTGCC TGCATCCTGC CGGGAAGGCA CTTGCTCGAC CTGCGTGGCA AAGTTGGAAA
     1441 AAGGGAACGT AGACCAACAG GACCAGAGCT TCTTGGATGA CGACCAGATT GAAAAGGGTT
     1501 ACATTCTGAT CTGTAAAGCG TACCCACTGT CAGATTGTAT TATTCGGACT CATGCGGAAG
     1561 ACCAGTTATA TGGGGGCTCT GGAGGTTCTG GAGGTTCTGG TGGGTCCGCG AGTAGCGAAG
     1621 ACGTTATCAA AGAGTTCATG CGTTTCAAAG TTCGTATGGA AGGTTCCGTT AACGGTCACG
     1681 AGTTCGAAAT CGAAGGTGAA GGTGAAGGTC GTCCGTACGA AGGTACCCAG ACCGCTAAAC
     1741 TGAAAGTTAC CAAAGGTGGT CCGCTGCCGT TCGCTTGGGA CATCCTGTCC CCGCAGTTCC
     1801 AGTACGGTTC CAAAGCTTAC GTTAAACACC CGGCTGACAT CCCGGACTAC CTGAAACTGT
     1861 CCTTCCCGGA AGGTTTCAAA TGGGAACGTG TTATGAACTT CGAAGACGGT GGTGTTGTTA
     1921 CCGTTACCCA GGACTCCTCC CTGCAAGACG GTGAGTTCAT CTACAAAGTT AAACTGCGTG
     1981 GTACCAACTT CCCGTCCGAC GGTCCGGTTA TGCAGAAAAA AACCATGGGT TGGGAAGCTT
     2041 CCACCGAACG TATGTACCCG GAAGACGGTG CTCTGAAAGG TGAAATCAAA ATGCGTCTGA
     2101 AACTGAAAGA CGGTGGTCAC TACGACGCTG AAGTTAAAAC CACCTACATG GCTAAAAAAC
     2161 CGGTTCAGCT GCCGGGTGCT TACAAAACCG ACATCAAACT GGACATCACC TCCCACAACG
     2221 AAGACTACAC CATCGTTGAA CAGTACGAAC GTGCTGAAGG TCGTCACTCC ACCGGTGCTT
     2281 AAgaattcTG ATAATAATCT AGACCAGGCA TCAAATAAAA CGAAAGGCTC AGTCGAAAGA
     2341 CTGGGCCTTT CGTTTTATCT GTTGTTTGTC GGTGAACGCT CTCTACTAGA GTCACACTGG
     2401 CTCACCTTCG GGTGGGCCTT TCTGCGTTTA TAGGTACCCG TGGATCCTCT AGAGTCGACC
     2461 TGCAGGCATG CAAGCTTTTT ACGGCTAGCT CAGTCCTAGG TATAGTGCTA GCCCAGCCAG
     2521 AGAAACACTC TTTAACAGGG GGTAGTATGA TGTCTCGTTT AGATAAAAGT AAAGTGATTA
     2581 ACAGCGCATT AGAGCTGCTT AATGAGGTCG GAATCGAAGG TTTAACAACC CGTAAACTCG
     2641 CCCAGAAGCT AGGTGTAGAG CAGCCTACAT TGTATTGGCA TGTAAAAAAT AAGCGGGCTT
     2701 TGCTCGACGC CTTAGCCATT GAGATGTTAG ATAGGCACCA TACTCACTTT TGCCCTTTAG
     2761 AAGGGGAAAG CTGGCAAGAT TTTTTACGTA ATAACGCTAA AAGTTTTAGA TGTGCTTTAC
     2821 TAAGTCATCG CGATGGAGCA AAAGTACATT TAGGTACACG GCCTACAGAA AAACAGTATG
     2881 AAACTCTCGA AAATCAATTA GCCTTTTTAT GCCAACAAGG TTTTTCACTA GAGAATGCAT
     2941 TATATGCACT CAGCGCAGTG GGGCATTTTA CTTTAGGTTG CGTATTGGAA GATCAAGAGC
     3001 ATCAAGTCGC TAAAGAAGAA AGGGAAACAC CTACTACTGA TAGTATGCCG CCATTATTAC
     3061 GACAAGCTAT CGAATTATTT GATCACCAAG GTGCAGAGCC AGCCTTCTTA TTCGGCCTTG
     3121 AATTGATCAT ATGCGGATTA GAAAAACAAC TTAAATGTGA AAGTGGGTCT TAAGGCATCA
     3181 AATAAAACGA AAGGCTCAGT CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT
     3241 GAACGCTCTC CTGAGTAGGA CAAATCCGCC GCCCTAGACC TAGGCGTTCG GCTGCGGCGA
     3301 GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA
     3361 GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG
     3421 CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT
     3481 CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC
     3541 CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT
     3601 TCGGGAAGCG TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC
     3661 GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA
     3721 TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA
     3781 GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG
     3841 TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG
     3901 CCAGTTACCT T
//