LOCUS       Recall-miniCMV-sfGFP        3453 bp    DNA     circular UNA 13-DEC-2019
DEFINITION  GoldenGate ligation of miniCMV-sfGFP into Recall 4.0.
ACCESSION   AA158
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     ligation        1..4
                     /label="Ligation"
     terminator      10..534
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="rb_glob_PA term"
                     /Transferred_Similarity="99.05%"
                     /label="rb_glob_PA term"
     misc_feature    complement(555..561)
                     /created_by="User"
                     /label="SapI"
     misc_feature    567..573
                     /created_by="User"
                     /label="SapI"
     misc_feature    complement(601..1220)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="pBR322 origin"
                     /Transferred_Similarity="100.00%"
                     /label="pBR322 origin"
                     /note="Geneious type: origin of replication"
     marker          complement(1375..2235)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp marker"
                     /Transferred_Similarity="99.88%"
                     /label="amp marker"
     promoter        complement(2277..2305)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="amp prom"
                     /Transferred_Similarity="100.00%"
                     /label="amp prom"
     terminator      complement(2318..2545)
                     /Imported_from="<a
                     href=""http://wishart.biology.ualberta.ca/PlasMapper"">Pla
                     sMapper</a>"
                     /Transferred_From="bGH_PA term"
                     /Transferred_Similarity="99.56%"
                     /label="bGH_PA term"
     motif           complement(2572..2577)
                     /created_by="User"
                     /label="BsmBI"
     motif           2603..2608
                     /created_by="User"
                     /label="BsmBI"
     misc_feature    complement(2616..2621)
                     /created_by="User"
                     /label="BbsI"
     misc_feature    2647..2652
                     /created_by="User"
                     /label="BbsI"
     promoter        2659..2719
                     /created_by="User"
                     /label="miniCMV"
     gene            2737..3453
                     /created_by="User"
                     /label="sfGFP"
ORIGIN      
        1 aggtccggcc gcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc
       61 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac
      121 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
      181 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
      241 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccat atgctggctg
      301 ccatgaacaa aggtggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
      361 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
      421 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
      481 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgaa gatccctcga
      541 cctgcaggtc accagaagag ctaacggctc ttccaagcgg gccaggaacc gtaaaaaggc
      601 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      661 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      721 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      781 tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt
      841 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      901 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      961 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1021 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct
     1081 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     1141 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     1201 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     1261 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     1321 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     1381 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     1441 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     1501 tgcaatgata ccgcgtgacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     1561 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     1621 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     1681 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     1741 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     1801 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     1861 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     1921 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     1981 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     2041 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     2101 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     2161 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     2221 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     2281 tctcatgagc ggatacatat ttgaatgtat ttagtggcca tagagcccac cgcatcccca
     2341 gcatgcctgc tattgacttc ccaatcctcc cccttgctgt cctgccccac cccacccccc
     2401 agaatagaat gacacctact cagacaatgc gatgcaattt cctcatttta ttaggaaagg
     2461 acagtgggag tggcaccttc cagggtcaag gaaggcacgg gggaggggca aacaacagat
     2521 ggctggcaac tagaaggcac agtcgaggct gatcagcggg tttaaaggag cgagacgaag
     2581 gctcactgtg cagactggag cgcgtctcct actatgtctt cttactgagg atattgcctg
     2641 aagctggaag acgtaatggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt
     2701 tagtgaaccg tcagatcgcc tggagaattc gccaccatga gcaaaggaga agaacttttc
     2761 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
     2821 gtcagaggag agggtgaagg tgatgctaca aacggaaaac tcacccttaa atttatttgc
     2881 actactggaa aactacctgt tccatggcca acacttgtca ctactctgac ctatggtgtt
     2941 caatgctttt cccgttatcc ggatcacatg aaacggcatg actttttcaa gagtgccatg
     3001 cccgaaggtt atgtacagga acgcactata tctttcaaag atgacgggac ctacaagacg
     3061 cgtgctgaag tcaagtttga aggtgatacc cttgttaatc gtatcgagtt aaaaggtatt
     3121 gattttaaag aagatggaaa cattctcgga cacaaactcg agtacaactt taactcacac
     3181 aatgtataca tcacggcaga caaacaaaag aatggaatca aagctaactt caaaattcgc
     3241 cacaacgttg aagatggttc cgttcaacta gcagaccatt atcaacaaaa tactccaatt
     3301 ggcgatggcc ctgtcctttt accagacaac cattacctgt cgacacaatc tgtcctttcg
     3361 aaagatccca acgaaaagcg tgaccacatg gtccttcttg agtttgtaac tgctgctggg
     3421 attacacatg gcatggatga gctctacaaa taa
//
