LOCUS       Exported                5311 bp ds-DNA     circular SYN 04-SEP-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Yiping Qi Lab
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, Dec 14, 2019 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     misc_feature    2..29
                     /label=FUS_A_left
                     /note="FUS_A_left"
     misc_feature    2..15
                     /label=FUS_A_left
                     /note="FUS_A_left"
     misc_feature    17
                     /label=CUT 1
                     /note="CUT 1"
     misc_feature    complement(45..320)
                     /label=lacZ'
                     /note="lacZ'"
     promoter        complement(321..442)
                     /label=lacZ promoter
                     /note="lacZ promoter"
     misc_feature    459..471
                     /label=FUS_A_right
                     /note="FUS_A_right"
     misc_feature    495..594
                     /label=attL2
     primer_bind     complement(611..632)
                     /label=T7
     misc_feature    complement(611..630)
                     /label=T7
                     /note="T7"
     primer_bind     complement(631..651)
                     /label=M13-rev
     misc_feature    complement(635..651)
                     /label=M13-R
     misc_feature    729..862
                     /label=Spn promoter
     misc_feature    863..1873
                     /label=SpnR
     misc_feature    1940..2613
                     /label=pUC origin
     primer_bind     3152..3169
                     /label=M13-fwd
     misc_feature    3153..3168
                     /label=M13-F
     misc_feature    3171..3283
                     /label=attL5
     misc_feature    3222..3246
                     /label=GW1
                     /note="GW1"
     primer_bind     3289..3326
                     /label=Primer 1
     promoter        3309..5306
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     primer_bind     complement(join(5284..5311,1..15))
                     /label=Primer 2
ORIGIN
        1 cccaagtggt ggctatcgag accggcgccg ctacagggcg cgtcccattc gccattcagg
       61 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
      121 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
      181 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt
      241 accgggcccc ccctcgaggt cctccagctt ttgttccctt tagtgagggt taattgcgcg
      301 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
      361 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
      421 actcacatta attgcgttgc gctcactgcc cgctttccac cggtggtctc tggacactag
      481 taagggcgaa ttcgacccag ctttcttgta caaagttggc attataaaaa ataattgctc
      541 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
      601 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
      661 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgcct
      721 cctctggacc agccaggaca gaaatgcctc gacttcgctg ctgcccaagg ttgccgggtg
      781 acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct gttcggttcg
      841 taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct tgaccgaacg
      901 cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt tttttggggt
      961 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat
     1021 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat
     1081 catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     1141 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     1201 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     1261 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     1321 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     1381 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     1441 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     1501 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     1561 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     1621 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     1681 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     1741 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     1801 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt
     1861 agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga gttacgcgtc
     1921 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1981 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2041 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2101 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     2161 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     2221 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     2281 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     2341 atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     2401 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     2461 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     2521 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     2581 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     2641 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2701 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     2761 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     2821 gggcagtgag cgcaacgcaa ttaatacgcg taccgctcgc caggaagagt ttgtagaaac
     2881 gcaaaaaggc catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc
     2941 gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc
     3001 ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
     3061 tcttccgact gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac
     3121 gcttgcatgg atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg
     3181 gcccaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca aattgatgag
     3241 caatgctttt ttataatgcc aactttgtat acaaaagttg ccccatggcg ttccctctag
     3301 ataacgcact gcagtgcagc gtgacccggt cgtgcccctc tctagagata atgagcattg
     3361 catgtctaag ttataaaaaa ttaccacata ttttttttgt cacacttgtt tgaagtgcag
     3421 tttatctatc tttatacata tatttaaact ttactctacg aataatataa tctatagtac
     3481 tacaataata tcagtgtttt agagaatcat ataaatgaac agttagacat ggtctaaagg
     3541 acaattgagt attttgacaa caggactcta cagttttatc tttttagtgt gcatgtgttc
     3601 tccttttttt ttgcaaatag cttcacctat ataatacttc atccatttta ttagtacatc
     3661 catttagggt ttagggttaa tggtttttat agactaattt ttttagtaca tctattttat
     3721 tctattttag cctctaaatt aagaaaacta aaactctatt ttagtttttt tatttaataa
     3781 tttagatata aaatagaata aaataaagtg actaaaaatt aaacaaatac cctttaagaa
     3841 attaaaaaaa ctaaggaaac atttttcttg tttcgagtag ataatgccag cctgttaaac
     3901 gccgtcgacg agtctaacgg acaccaacca gcgaaccagc agcgtcgcgt cgggccaagc
     3961 gaagcagacg gcacggcatc tctgtcgctg cctctggacc cctctcgaga gttccgctcc
     4021 accgttggac ttgctccgct gtcggcatcc agaaattgcg tggcggagcg gcagacgtga
     4081 gccggcacgg caggcggcct cctcctcctc tcacggcacc ggcagctacg ggggattcct
     4141 ttcccaccgc tccttcgctt tcccttcctc gcccgccgta ataaatagac accccctcca
     4201 caccctcttt ccccaacctc gtgttgttcg gagcgcacac acacacaacc agatctcccc
     4261 caaatccacc cgtcggcacc tccgcttcaa ggtacgccgc tcgtcctccc cccccccccc
     4321 ctctctacct tctctagatc ggcgttccgg tccatggtta gggcccggta gttctacttc
     4381 tgttcatgtt tgtgttagat ccgtgtttgt gttagatccg tgctgctagc gttcgtacac
     4441 ggatgcgacc tgtacgtcag acacgttctg attgctaact tgccagtgtt tctctttggg
     4501 gaatcctggg atggctctag ccgttccgca gacgggatcg atttcatgat tttttttgtt
     4561 tcgttgcata gggtttggtt tgcccttttc ctttatttca atatatgccg tgcacttgtt
     4621 tgtcgggtca tcttttcatg cttttttttg tcttggttgt gatgatgtgg tctggttggg
     4681 cggtcgttct agatcggagt agaattaatt ctgtttcaaa ctacctggtg gatttattaa
     4741 ttttggatct gtatgtgtgt gccatacata ttcatagtta cgaattgaag atgatggatg
     4801 gaaatatcga tctaggatag gtatacatgt tgatgcgggt tttactgatg catatacaga
     4861 gatgcttttt gttcgcttgg ttgtgatgat gtggtgtggt tgggcggtcg ttcattcgtt
     4921 ctagatcgga gtagaatact gtttcaaact acctggtgta tttattaatt ttggaactgt
     4981 atgtgtgtgt catacatctt catagttacg agtttaagat ggatggaaat atcgatctag
     5041 gataggtata catgttgatg tgggttttac tgatgcatat acatgatggc atatgcagca
     5101 tctattcata tgctctaacc ttgagtacct atctattata ataaacaagt atgttttata
     5161 attattttga tcttgatata cttggatgat ggcatatgca gcagctatat gtggattttt
     5221 ttagccctgc cttcatacgc tatttatttg cttggtactg tttcttttgt cgatgctcac
     5281 cctgttgttt ggtgttactt ctgcagggat c
//
