LOCUS       Exported               18516 bp ds-DNA     circular SYN 07-NOV-2018
DEFINITION  AssembledSeq.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 16, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..18516
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          5338..10225
                     /label=V
     source          5338..7266
                     /label=V
     CDS             complement(578..1207)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    2507..2531
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     2734..2750
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2817..3196
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     misc_feature    3218..3303
                     /label=esgRNA
     terminator      3304..3323
                     /label=SUP4 terminator
                     /note="transcription terminator for the S. cerevisiae SUP4 
                     tRNA gene"
     promoter        3336..5327
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             5338..5934
                     /codon_start=1
                     /label=A3A
                     /translation="MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTS
                     VKMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSW
                     GCAGEVRAFLQENTHVRLRIFAARIYDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWD
                     TFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN"
     CDS             5935..5982
                     /codon_start=1
                     /label=XTEN linker
                     /translation="SGSETPGTSESATPES"
     CDS             5983..6078
                     /codon_start=1
                     /label=32aa linker
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             6079..6576
                     /codon_start=1
                     /label=ecTadA(wt)
                     /translation="SEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEGWN
                     RPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRVVFG
                     ARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQKKAQS
                     STD"
     CDS             6577..6672
                     /codon_start=1
                     /label=32aa linker
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             6673..7170
                     /codon_start=1
                     /label=ecTadA*(7.10)
                     /translation="SEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWN
                     RAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFG
                     VRNAKTGAAGSLMDVLHYPGMNHRVEITEGILADECAALLCYFFRMPRQVFNAQKKAQS
                     STD"
     CDS             7171..7266
                     /codon_start=1
                     /label=32aa linker
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             7267..11367
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)-NG
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    10594..10596
                     /label=L1111R
     misc_feature    10666..10668
                     /label=D1135V
     misc_feature    10915..10917
                     /label=G1218R
     misc_feature    10918..10920
                     /label=E1219F
     misc_feature    11227..11229
                     /label=A1322R
     misc_feature    11266..11267
                     /label=R1335V
     misc_feature    11272..11274
                     /label=T1337R
     CDS             11368..11415
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     misc_feature    11416..11697
                     /label=UGI
     CDS             11425..11436
                     /codon_start=1
                     /label=SGGS
                     /translation="SGGS"
     CDS             11686..11697
                     /codon_start=1
                     /label=SGGS
                     /translation="SGGS"
     misc_feature    11698..11718
                     /label=sv40 NLS
     terminator      11728..12362
                     /label=E9ter
     primer_bind     complement(12377..12393)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12401..12417
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12425..12455)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12470..12491
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12682..13359
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             13427..14452
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    14492..14666
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    14744..14768
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             15193..15987
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      16074..16662
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    16848..16988
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      17332..17526
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(join(17592..18516,1..149))
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
ORIGIN
        1 tcagcggccg cttgccacga ttcaccgggg cgtgcttctg cctcgatgcg ttgccgctgg
       61 gcggcctgcg cggccttcaa cttctccacc aggtcatcac ccagcgccgc gccgatttgt
      121 accgggccgg atggtttgcg accgctcacg ccgattcctc gggcttgggg gttccagtgc
      181 cattgcaggg ccggcagaca acccagccgc ttacgcctgg ccaaccgccc gttcctccac
      241 acatggggca ttccacggcg tcggtgcctg gttgttcttg attttccatg ccgcctcctt
      301 tagccgctaa aattcatcta ctcatttatt catttgctca tttactctgg tagctgcgcg
      361 atgtattcag atagcagctc ggtaatggtc ttgccttggc gtaccgcgta catcttcagc
      421 ttggtgtgat cctccgccgg caactgaaag ttgacccgct tcatggctgg cgtgtctgcc
      481 aggctggcca acgttgcagc cttgctgctg cgtgcgctcg gacggccggc acttagcgtg
      541 tttgtgcttt tgctcatttt ctctttacct cattaactca aatgagtttt gatttaattt
      601 cagcggccag cgcctggacc tcgcgggcag cgtcgccctc gggttctgat tcaagaacgg
      661 ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg cgtgatacgg gactcaagaa
      721 tgggcagctc gtacccggcc agcgcctcgg caacctcacc gccgatgcgc gtgcctttga
      781 tcgcccgcga cacgacaaag gccgcttgta gccttccatc cgtgacctca atgcgctgct
      841 taaccagctc caccaggtcg gcggtggccc atatgtcgta agggcttggc tgcaccggaa
      901 tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa gtccgccgcc tggggcgctc
      961 cgtcgatcac tacgaagtcg cgccggccga tggccttcac gtcgcggtca atcgtcgggc
     1021 ggtcgatgcc gacaacggtt agcggttgat cttcccgcac ggccgcccaa tcgcgggcac
     1081 tgccctgggg atcggaatcg actaacagaa catcggcccc ggcgagttgc agggcgcggg
     1141 ctagatgggt tgcgatggtc gtcttgcctg acccgccttt ctggttaagt acagcgataa
     1201 ccttcatgcg ttccccttgc gtatttgttt atttactcat cgcatcatat acgcagcgac
     1261 cgcatgacgc aagctgtttt actcaaatac acatcacctt tttagacggc ggcgctcggt
     1321 ttcttcagcg gccaagctgg ccggccaggc cgccagcttg gcatcagaca aaccggccag
     1381 gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc tcgaacacgt acccggccgc
     1441 gatcatctcc gcctcgatct cttcggtaat gaaaaacggt tcgtcctggc cgtcctggtg
     1501 cggtttcatg cttgttcctc ttggcgttca ttctcggcgg ccgccagggc gtcggcctcg
     1561 gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct cggtgggcgt cacttcctcg
     1621 ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc caagcagtgc agccgcctct
     1681 ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt gcgcgatctg tgccggggtg
     1741 agggtagggc gggggccaaa cttcacgcct cgggccttgg cggcctcgcg cccgctccgg
     1801 gtgcggtcga tgattaggga acgctcgaac tcggcaatgc cggcgaacac ggtcaacacc
     1861 atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg ccaggctacg caggcccgcg
     1921 ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc gggtgctgcg ggccaggcgg
     1981 tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt ggtcaagcat cctggccagc
     2041 tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa acagcttggt gcagccggcc
     2101 gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt cggtgctgac gcgggcatag
     2161 cccagcaggc cagcggcggc gctcttgttc atggcgtaat gtctccggtt ctagtcgcaa
     2221 gtattctact ttatgcgact aaaacacgcg acaagaaaac gccaggaaaa gggcagggcg
     2281 gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt tttcagaaga cggctgcact
     2341 gaacgtcaga agccgactgc actatagcag cggaggggtt ggatcaaagt actttgatcc
     2401 cgaggggaac cctgtggttg gcatgcacat acaaatggac gaacggataa accttttcac
     2461 gcccttttaa atatccgtta ttctaataaa cgctcttttc tcttaggttt acccgccaat
     2521 atatcctgtc aaacactgat agtttaaact gaaggcggga aacgacaatc tgatccaagc
     2581 tcaagctgct ctagcattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     2641 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     2701 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctta
     2761 gtaattcatc caggtcacca agttctagga ttttcagaac tgcaacttat tttatcaagg
     2821 aatctttaaa catacgaaca gatcacttaa agttcttctg aagcaactta aagttatcag
     2881 gcatgcatgg atcttggagg aatcagatgt gcagtcaggg accatagcac aagacaggcg
     2941 tcttctactg gtgctaccag caaatgctgg aagccgggaa cactgggtac gttggaaacc
     3001 acgtgatgtg aagaagtaag ataaactgta ggagaaaagc atttcgtagt gggccatgaa
     3061 gcctttcagg acatgtattg cagtatgggc cggcccatta cgcaattgga cgacaacaaa
     3121 gactagtatt agtaccacct cggctatcca catagatcaa agctgattta aaagagttgt
     3181 gcagatgatc cgtggcgtga gaccctgcag ggtctctgtt taagagctat gctggaaaca
     3241 gcatagcaag tttaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg
     3301 tgcttttttt gttttttatg tctaagcttg catgcctgca gtgcagcgtg acccggtcgt
     3361 gcccctctct agagataatg agcattgcat gtctaagtta taaaaaatta ccacatattt
     3421 tttttgtcac acttgtttga agtgcagttt atctatcttt atacatatat ttaaacttta
     3481 ctctacgaat aatataatct atagtactac aataatatca gtgttttaga gaatcatata
     3541 aatgaacagt tagacatggt ctaaaggaca attgagtatt ttgacaacag gactctacag
     3601 ttttatcttt ttagtgtgca tgtgttctcc tttttttttg caaatagctt cacctatata
     3661 atacttcatc cattttatta gtacatccat ttagggttta gggttaatgg tttttataga
     3721 ctaatttttt tagtacatct attttattct attttagcct ctaaattaag aaaactaaaa
     3781 ctctatttta gtttttttat ttaataattt agatataaaa tagaataaaa taaagtgact
     3841 aaaaattaaa caaataccct ttaagaaatt aaaaaaacta aggaaacatt tttcttgttt
     3901 cgagtagata atgccagcct gttaaacgcc gtcgacgagt ctaacggaca ccaaccagcg
     3961 aaccagcagc gtcgcgtcgg gccaagcgaa gcagacggca cggcatctct gtcgctgcct
     4021 ctggacccct ctcgagagtt ccgctccacc gttggacttg ctccgctgtc ggcatccaga
     4081 aattgcgtgg cggagcggca gacgtgagcc ggcacggcag gcggcctcct cctcctctca
     4141 cggcacggca gctacggggg attcctttcc caccgctcct tcgctttccc ttcctcgccc
     4201 gccgtaataa atagacaccc cctccacacc ctctttcccc aacctcgtgt tgttcggagc
     4261 gcacacacac acaaccagat ctcccccaaa tccacccgtc ggcacctccg cttcaaggta
     4321 cgccgctcgt cctccccccc cccccctctc taccttctct agatcggcgt tccggtccat
     4381 ggttagggcc cggtagttct acttctgttc atgtttgtgt tagatccgtg tttgtgttag
     4441 atccgtgctg ctagcgttcg tacacggatg cgacctgtac gtcagacacg ttctgattgc
     4501 taacttgcca gtgtttctct ttggggaatc ctgggatggc tctagccgtt ccgcagacgg
     4561 gatcgatttc atgatttttt ttgtttcgtt gcatagggtt tggtttgccc ttttccttta
     4621 tttcaatata tgccgtgcac ttgtttgtcg ggtcatcttt tcatgctttt ttttgtcttg
     4681 gttgtgatga tgtggtctgg ttgggcggtc gttctagatc ggagtagaat tctgtttcaa
     4741 actacctggt ggatttatta attttggatc tgtatgtgtg tgccatacat attcatagtt
     4801 acgaattgaa gatgatggat ggaaatatcg atctaggata ggtatacatg ttgatgcggg
     4861 ttttactgat gcatatacag agatgctttt tgttcgcttg gttgtgatga tgtggtgtgg
     4921 ttgggcggtc gttcattcgt tctagatcgg agtagaatac tgtttcaaac tacctggtgt
     4981 atttattaat tttggaactg tatgtgtgtg tcatacatct tcatagttac gagtttaaga
     5041 tggatggaaa tatcgatcta ggataggtat acatgttgat gtgggtttta ctgatgcata
     5101 tacatgatgg catatgcagc atctattcat atgctctaac cttgagtacc tatctattat
     5161 aataaacaag tatgttttat aattattttg atcttgatat acttggatga tggcatatgc
     5221 agcagctata tgtggatttt tttagccctg ccttcatacg ctatttattt gcttggtact
     5281 gtttcttttg tcgatgctca ccctgttgtt tggtgttact tctgcagccc taggcctatg
     5341 gaggccagcc cggctagcgg cccaaggcat ctcatggacc cgcacatctt caccagcaac
     5401 ttcaacaacg gcatcggcag gcacaagacc tacttgtgct acgaggtgga gaggctcgac
     5461 aacggaacct ccgtgaagat ggaccaacac agggggttcc tccacaacca agccaagaac
     5521 ctcctctgcg gcttctacgg caggcacgcc gagttgaggt tcctcgactt ggtgccatcc
     5581 ctccaactcg atccagccca aatctaccgc gtgacctggt tcatctcctg gtccccatgc
     5641 ttctcctggg gttgcgccgg cgaggttcgg gctttcctcc aagaaaacac ccacgtccgc
     5701 ctccgcattt tcgccgccag gatctatgat tacgaccctc tctacaagga ggccctccag
     5761 atgctgcggg acgccggtgc tcaggtgagt atcatgacct acgacgagtt caagcactgc
     5821 tgggacacct tcgttgacca ccagggctgc ccattccaac catgggacgg tctggatgaa
     5881 cacagccaag ccttgtccgg caggctccgg gccatcctcc aaaaccaggg gaactccggg
     5941 agcgagacgc caggcacctc cgagtcggcc accccagaat cttccggcgg atcttctgga
     6001 ggatctagcg gctccgagac accaggaaca tccgaatccg ctacaccaga gtcttctgga
     6061 ggatctagcg gaggatcttc cgaggtggag ttctctcacg agtattggat gaggcacgct
     6121 cttacacttg ctaagagagc ttgggacgaa agagaagtgc cagttggcgc cgttcttgtg
     6181 cataataata gggtgatcgg cgagggttgg aatagaccaa ttggaaggca tgatccaaca
     6241 gctcacgcag agattatggc tctcagacaa ggcggcctcg ttatgcagaa ctacaggctc
     6301 attgacgcta cactctacgt gacactcgaa ccttgcgtta tgtgcgccgg agctatgatt
     6361 cattctagga ttggcagggt cgtgtttgga gctagggacg ctaaaacagg agccgccgga
     6421 tctcttatgg acgtgttgca tcatccaggc atgaaccata gggtggagat tacagagggc
     6481 attcttgcag acgagtgcgc tgctcttctt tccgatttct tcaggatgag aaggcaggag
     6541 attaaggccc agaagaaggc tcagtcttct acagatagcg gaggatcttc cggaggatct
     6601 agcggctccg agacaccagg aacatccgaa agcgctacac cagaatctag cggaggctct
     6661 tccggaggat cttctgaagt ggagttctcc cacgagtatt ggatgaggca cgctcttaca
     6721 cttgctaaaa gggctaggga cgaaagggaa gttccagttg gagctgttct cgtgctcaat
     6781 aacagggtga ttggcgaggg ttggaataga gccattggac tccatgatcc aacagctcac
     6841 gcagagatta tggctcttag acaaggcggc ctcgttatgc agaattacag actcatcgac
     6901 gccacactct acgttacctt cgaaccttgc gttatgtgcg ccggagctat gatccattct
     6961 aggattggca gggtcgtgtt cggcgttaga aacgctaaga caggagctgc aggctctctt
     7021 atggacgttc ttcattaccc aggcatgaat catagagtgg agatcacaga aggcattctt
     7081 gcagacgagt gcgcagctct cctttgctat ttcttcagga tgccgaggca agttttcaac
     7141 gctcagaaga aggcccagtc ttctacagat tccggcggat cttctggagg atctagcggc
     7201 tccgagacac caggaacatc cgaatccgct acaccagagt cttctggagg atctagcgga
     7261 ggatctgaca agaagtactc gatcggcctc gccattggga ctaactctgt tggctgggcc
     7321 gtgatcaccg acgagtacaa ggtgccctca aagaagttca aggtcctggg caacaccgat
     7381 cggcattcca tcaagaagaa tctcattggc gctctcctgt tcgacagcgg cgagacggct
     7441 gaggctacgc ggctcaagcg caccgcccgc aggcggtaca cgcgcaggaa gaatcgcatc
     7501 tgctacctgc aggagatttt ctccaacgag atggcgaagg ttgacgattc tttcttccac
     7561 aggctggagg agtcattcct cgtggaggag gataagaagc acgagcggca tccaatcttc
     7621 ggcaacattg tcgacgaggt tgcctaccac gagaagtacc ctacgatcta ccatctgcgg
     7681 aagaagctcg tggactccac agataaggcg gacctccgcc tgatctacct cgctctggcc
     7741 cacatgatta agttcagggg ccatttcctg atcgaggggg atctcaaccc ggacaatagc
     7801 gatgttgaca agctgttcat ccagctcgtg cagacgtaca accagctctt cgaggagaac
     7861 cccattaatg cgtcaggcgt cgacgcgaag gctatcctgt ccgctaggct ctcgaagtct
     7921 cggcgcctcg agaacctgat cgcccagctg ccgggcgaga agaagaacgg cctgttcggg
     7981 aatctcattg cgctcagcct ggggctcacg cccaacttca agtcgaattt cgatctcgct
     8041 gaggacgcca agctgcagct ctccaaggac acatacgacg atgacctgga taacctcctg
     8101 gcccagatcg gcgatcagta cgcggacctg ttcctcgctg ccaagaatct gtcggacgcc
     8161 atcctcctgt ctgatattct cagggtgaac accgagatta cgaaggctcc gctctcagcc
     8221 tccatgatca agcgctacga cgagcaccat caggatctga ccctcctgaa ggcgctggtc
     8281 aggcagcagc tccccgagaa gtacaaggag atcttcttcg atcagtcgaa gaacggctac
     8341 gctgggtaca ttgacggcgg ggcctctcag gaggagttct acaagttcat caagccgatt
     8401 ctggagaaga tggacggcac ggaggagctg ctggtgaagc tcaatcgcga ggacctcctg
     8461 aggaagcagc ggacattcga taacggcagc atcccacacc agattcatct cggggagctg
     8521 cacgctatcc tgaggaggca ggaggacttc taccctttcc tcaaggataa ccgcgagaag
     8581 atcgagaaga ttctgacttt caggatcccg tactacgtcg gcccactcgc taggggcaac
     8641 tcccgcttcg cttggatgac ccgcaagtca gaggagacga tcacgccgtg gaacttcgag
     8701 gaggtggtcg acaagggcgc tagcgctcag tcgttcatcg agaggatgac gaatttcgac
     8761 aagaacctgc caaatgagaa ggtgctccct aagcactcgc tcctgtacga gtacttcaca
     8821 gtctacaacg agctgactaa ggtgaagtat gtgaccgagg gcatgaggaa gccggctttc
     8881 ctgtctgggg agcagaagaa ggccatcgtg gacctcctgt tcaagaccaa ccggaaggtc
     8941 acggttaagc agctcaagga ggactacttc aagaagattg agtgcttcga ttcggtcgag
     9001 atctctggcg ttgaggaccg cttcaacgcc tccctgggga cctaccacga tctcctgaag
     9061 atcattaagg ataaggactt cctggacaac gaggagaatg aggatatcct cgaggacatt
     9121 gtgctgacac tcactctgtt cgaggaccgg gagatgatcg aggagcgcct gaagacttac
     9181 gcccatctct tcgatgacaa ggtcatgaag cagctcaaga ggaggaggta caccggctgg
     9241 gggaggctga gcaggaagct catcaacggc attcgggaca agcagtccgg gaagacgatc
     9301 ctcgacttcc tgaagagcga tggcttcgcg aaccgcaatt tcatgcagct gattcacgat
     9361 gacagcctca cattcaagga ggatatccag aaggctcagg tgagcggcca gggggactcg
     9421 ctgcacgagc atatcgcgaa cctcgctggc tcgccagcta tcaagaaggg gattctgcag
     9481 accgtgaagg ttgtggacga gctggtgaag gtcatgggca ggcacaagcc tgagaacatc
     9541 gtcattgaga tggcccggga gaatcagacc acgcagaagg gccagaagaa ctcacgcgag
     9601 aggatgaaga ggatcgagga gggcattaag gagctggggt cccagatcct caaggagcac
     9661 ccggtggaga acacgcagct gcagaatgag aagctctacc tgtactacct ccagaatggc
     9721 cgcgatatgt atgtggacca ggagctggat attaacaggc tcagcgatta cgacgtcgat
     9781 catatcgttc cacagtcatt cctgaaggat gactccattg acaacaaggt cctcaccagg
     9841 tcggacaaga accggggcaa gtctgataat gttccttcag aggaggtcgt taagaagatg
     9901 aagaactact ggcgccagct cctgaatgcc aagctgatca cgcagcggaa gttcgataac
     9961 ctcacaaagg ctgagagggg cgggctctct gagctggaca aggcgggctt catcaagagg
    10021 cagctggtcg agacacggca gatcactaag cacgttgcgc agattctcga ctcacggatg
    10081 aacactaagt acgatgagaa tgacaagctg atccgcgagg tgaaggtcat caccctgaag
    10141 tcaaagctcg tctccgactt caggaaggat ttccagttct acaaggttcg ggagatcaac
    10201 aattaccacc atgcccatga cgcgtacctg aacgcggtgg tcggcacagc tctgatcaag
    10261 aagtacccaa agctcgagag cgagttcgtg tacggggact acaaggttta cgatgtgagg
    10321 aagatgatcg ccaagtcgga gcaggagatt ggcaaggcta ccgccaagta cttcttctac
    10381 tctaacatta tgaatttctt caagacagag atcactctgg ccaatggcga gatccggaag
    10441 cgccccctca tcgagacgaa cggcgagacg ggggagatcg tgtgggacaa gggcagggat
    10501 ttcgcgaccg tcaggaaggt tctctccatg ccacaagtga atatcgtcaa gaagacagag
    10561 gtccagactg gcgggttctc taaggagtca attcggccta agcggaacag cgacaagctc
    10621 atcgcccgca agaaggactg ggatccgaag aagtacggcg ggttcgtcag ccccactgtg
    10681 gcctactcgg tcctggttgt ggcgaaggtt gagaagggca agtccaagaa gctcaagagc
    10741 gtgaaggagc tgctggggat cacgattatg gagcgctcca gcttcgagaa gaacccgatc
    10801 gatttcctgg aggcgaaggg ctacaaggag gtgaagaagg acctgatcat taagctcccc
    10861 aagtactcac tcttcgagct ggagaacggc aggaagcgga tgctggcttc cgctcgcttc
    10921 ctgcagaagg ggaacgagct ggctctgccg tccaagtatg tgaacttcct ctacctggcc
    10981 tcccactacg agaagctcaa gggcagcccc gaggacaacg agcagaagca gctgttcgtc
    11041 gagcagcaca agcattacct cgacgagatc attgagcaga tttccgagtt ctccaagcgc
    11101 gtgatcctgg ccgacgcgaa tctggataag gtcctctccg cgtacaacaa gcaccgcgac
    11161 aagccaatca gggagcaggc tgagaatatc attcatctct tcaccctgac gaacctcggc
    11221 gcccctcgtg ctttcaagta cttcgacaca actatcgatc gcaaggtata cagaagcact
    11281 aaggaggtcc tggacgcgac cctcatccac cagtcgatta ccggcctcta cgagacgcgc
    11341 atcgacctgt ctcagctcgg gggcgacaag cggccagcgg cgacgaagaa ggcggggcag
    11401 gcgaagaaga agaagacccg cgactccggc ggcagcacga acctctccga catcatcgag
    11461 aaggagacgg gcaagcagct cgtgatccag gagagcatcc tcatgctgcc ggaggaggtg
    11521 gaggaggtca tcggcaacaa gcccgagtcc gacatcctcg tgcacaccgc ctacgacgag
    11581 tccacggacg agaacgtcat gctcctgacg agcgacgctc cagagtacaa gccatgggct
    11641 ctcgtgatcc aggacagcaa cggcgagaac aagatcaaga tgctgtccgg cggctcccca
    11701 aagaagaagc ggaaggtgta ggagctcaga gctttcgttc gtatcatcgg tttcgacaac
    11761 gttcgtcaag ttcaatgcat cagtttcatt gcgcacacac cagaatccta ctgagtttga
    11821 gtattatggc attgggaaaa ctgtttttct tgtaccattt gttgtgcttg taatttactg
    11881 tgttttttat tcggttttcg ctatcgaact gtgaaatgga aatggatgga gaagagttaa
    11941 tgaatgatat ggtccttttg ttcattctca aattaatatt atttgttttt tctcttattt
    12001 gttgtgtgtt gaatttgaaa ttataagaga tatgcaaaca ttttgttttg agtaaaaatg
    12061 tgtcaaatcg tggcctctaa tgaccgaagt taatatgagg agtaaaacac ttgtagttgt
    12121 accattatgc ttattcacta ggcaacaaat atattttcag acctagaaaa gctgcaaatg
    12181 ttactgaata caagtatgtc ctcttgtgtt ttagacattt atgaactttc ctttatgtaa
    12241 ttttccagaa tccttgtcag attctaatca ttgctttata attatagtta tactcatgga
    12301 tttgtagttg agtatgaaaa tattttttaa tgcattttat gacttgccaa ttgattgaca
    12361 acgaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
    12421 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
    12481 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
    12541 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt ggctagagca
    12601 gcttgccaac atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt
    12661 ctcagaagac caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct
    12721 cggattccat tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg
    12781 cacctacaaa tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga
    12841 cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc
    12901 aaccacgtct tcaaagcaag tggattgatg tgataacatg gtggagcacg acactctcgt
    12961 ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca
    13021 acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
    13081 caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa
    13141 ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
    13201 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
    13261 tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag accttcctct
    13321 atataaggaa gttcatttca tttggagagg acacgctgaa atcaccagtc tctctctaca
    13381 aatctatctc tctcgagctt tcgcagatcc cggggggcaa tgagatatga aaaagcctga
    13441 actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct
    13501 gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg
    13561 atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg
    13621 gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg agtttagcga
    13681 gagcctgacc tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga
    13741 aaccgaactg cccgctgttc tacaaccggt cgcggaggct atggatgcga tcgctgcggc
    13801 cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac
    13861 tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt
    13921 gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc
    13981 cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct
    14041 gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc
    14101 ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca
    14161 gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccacgac tccgggcgta
    14221 tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga
    14281 tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg
    14341 gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact
    14401 cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaagaaat agagtagatg
    14461 ccgaccggat ctgtcgatcg acaagctcga gtttctccat aataatgtgt gagtagttcc
    14521 cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat ataagaaacc
    14581 cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac
    14641 caaaatccag tactaaaatc cagatccccc gaattaattc ggcgttaatt cagtacatta
    14701 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata
    14761 tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg
    14821 atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga
    14881 ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac
    14941 acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg
    15001 tgatcaccgc ggtttcaaaa tcggctccgt cgatactatg ttatacgcca actttgaaaa
    15061 caactttgaa aaagctgttt tctggtattt aaggttttag aatgcaagga acagtgaatt
    15121 ggagttcgtc ttgttataat tagcttcttg gggtatcttt aaatactgta gaaaagagga
    15181 aggaaataat aaatggctaa aatgagaata tcaccggaat tgaaaaaact gatcgaaaaa
    15241 taccgctgcg taaaagatac ggaaggaatg tctcctgcta aggtatataa gctggtggga
    15301 gaaaatgaaa acctatattt aaaaatgacg gacagccggt ataaagggac cacctatgat
    15361 gtggaacggg aaaaggacat gatgctatgg ctggaaggaa agctgcctgt tccaaaggtc
    15421 ctgcactttg aacggcatga tggctggagc aatctgctca tgagtgaggc cgatggcgtc
    15481 ctttgctcgg aagagtatga agatgaacaa agccctgaaa agattatcga gctgtatgcg
    15541 gagtgcatca ggctctttca ctccatcgac atatcggatt gtccctatac gaatagctta
    15601 gacagccgct tagccgaatt ggattactta ctgaataacg atctggccga tgtggattgc
    15661 gaaaactggg aagaagacac tccatttaaa gatccgcgcg agctgtatga ttttttaaag
    15721 acggaaaagc ccgaagagga acttgtcttt tcccacggcg acctgggaga cagcaacatc
    15781 tttgtgaaag atggcaaagt aagtggcttt attgatcttg ggagaagcgg cagggcggac
    15841 aagtggtatg acattgcctt ctgcgtccgg tcgatcaggg aggatatcgg ggaagaacag
    15901 tatgtcgagc tattttttga cttactgggg atcaagcctg attgggagaa aataaaatat
    15961 tatattttac tggatgaatt gttttagtac ctagaatgca tgaccaaaat cccttaacgt
    16021 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
    16081 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
    16141 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
    16201 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
    16261 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
    16321 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
    16381 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
    16441 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
    16501 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
    16561 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
    16621 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
    16681 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
    16741 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
    16801 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
    16861 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
    16921 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
    16981 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
    17041 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
    17101 caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt
    17161 ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc agccattttt
    17221 gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac
    17281 cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag ttatgcacag
    17341 gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga aaaatcgcct
    17401 tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta cggctttggg
    17461 ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat gtacgtgcta
    17521 tccacaggaa acagaccttt tcgacctttt tcccctgcta gggcaatttg ccctagcatc
    17581 tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg
    17641 actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg caggtcattt
    17701 gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc gctgccactg
    17761 cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc
    17821 aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc
    17881 agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc
    17941 tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct aatcaaggct
    18001 tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg ctgcatggca
    18061 acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg ctttccgcca
    18121 tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc
    18181 ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga aaccgccatc
    18241 agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga aacctctacg
    18301 tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg cttcgacaga
    18361 cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc atagagcatc
    18421 ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg
    18481 gctgccggcg gttgccggga ttctttgcgg attcga
//
