LOCUS       Exported               11330 bp ds-DNA     circular SYN 30-JUL-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 16, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
COMMENT     NEBuilder_Assembly
FEATURES             Location/Qualifiers
     source          1..11330
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..243
                     /label=V
     source          244..1946
                     /label=V
     source          1947..8094
                     /label=V
     source          8101..8397
                     /label=fragment
     source          8404..11330
                     /label=V
     promoter        24..2010
                     /label=Ubi promoter
     CDS             2017..2613
                     /codon_start=1
                     /label=A3A
                     /translation="MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTS
                     VKMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSW
                     GCAGEVRAFLQENTHVRLRIFAARIYDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWD
                     TFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN"
     CDS             2614..2661
                     /codon_start=1
                     /label=XTEN linker
                     /translation="SGSETPGTSESATPES"
     CDS             2662..2757
                     /codon_start=1
                     /label=32aa linker
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             2758..3255
                     /codon_start=1
                     /label=ecTadA(wt)
                     /translation="SEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEGWN
                     RPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRVVFG
                     ARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQKKAQS
                     STD"
     CDS             3256..3351
                     /codon_start=1
                     /label=32aa linker
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             3352..3849
                     /codon_start=1
                     /label=ecTadA*(7.10)
                     /translation="SEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWN
                     RAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFG
                     VRNAKTGAAGSLMDVLHYPGMNHRVEITEGILADECAALLCYFFRMPRQVFNAQKKAQS
                     STD"
     CDS             3850..3945
                     /codon_start=1
                     /label=32aa linker
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             3946..8046
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8047..8094
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8113..8361
                     /codon_start=1
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8374..8394
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8452..8628
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(9171..9187)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9195..9211
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9219..9249)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9264..9285
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9573..10161)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10332..11192)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11193..11297)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 caagctaatt cgagctcggt acctgacccg gtcgtgcccc tctctagaga taatgagcat
       61 tgcatgtcta agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc
      121 agtttatcta tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt
      181 actacaataa tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa
      241 ggacaattga gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt
      301 tctccttttt ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca
      361 tccatttagg gtttagggtt aatggttttt atagactaat ttttttagta catctatttt
      421 attctatttt agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat
      481 aatttagata taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag
      541 aaattaaaaa aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa
      601 acgccgtcga cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa
      661 gcgaagcaga cggcacggca tctctgtcgc tgcctctgga cccctctcga tcgagagttc
      721 cgctccaccg ttggacttgc tccgctgtcg gcatccagaa attgcgtggc ggagcggcag
      781 acgtgagccg gcacggcagg cggcctcctc ctcctctcac ggcaccggca gctacggggg
      841 attcctttcc caccgctcct tcgctttccc ttcctcgccc gccgtaataa atagacaccc
      901 cctccacacc ctctttcccc aacctcgtgt tgttcggagc gcacacacac acaaccagat
      961 ctcccccaaa tccacccgtc ggcacctccg cttcaaggta cgccgctcgt cctccccccc
     1021 cccccctctc taccttctct agatcggcgt tccggtccat ggttagggcc cggtagttct
     1081 acttctgttc atgtttgtgt tagatccgtg tttgtgttag atccgtgctg ctagcgttcg
     1141 tacacggatg cgacctgtac gtcagacacg ttctgattgc taacttgcca gtgtttctct
     1201 ttggggaatc ctgggatggc tctagccgtt ccgcagacgg gatcgatttc atgatttttt
     1261 ttgtttcgtt gcatagggtt tggtttgccc ttttccttta tttcaatata tgccgtgcac
     1321 ttgtttgtcg ggtcatcttt tcatgctttt ttttgtcttg gttgtgatga tgtggtctgg
     1381 ttgggcggtc gttctagatc ggagtagaat taattctgtt tcaaactacc tggtggattt
     1441 attaattttg gatctgtatg tgtgtgccat acatattcat agttacgaat tgaagatgat
     1501 ggatggaaat atcgatctag gataggtata catgttgatg cgggttttac tgatgcatat
     1561 acagagatgc tttttgttcg cttggttgtg atgatgtggt gtggttgggc ggtcgttcat
     1621 tcgttctaga tcggagtaga atactgtttc aaactacctg gtgtatttat taattttgga
     1681 actgtatgtg tgtgtcatac atcttcatag ttacgagttt aagatggatg gaaatatcga
     1741 tctaggatag gtatacatgt tgatgtgggt tttactgatg catatacatg atggcatatg
     1801 cagcatctat tcatatgctc taaccttgag tacctatcta ttataataaa caagtatgtt
     1861 ttataattat tttgatcttg atatacttgg atgatggcat atgcagcagc tatatgtgga
     1921 tttttttagc cctgccttca tacgctattt atttgcttgg tactgtttct tttgtcgatg
     1981 ctcaccctgt tgtttggtgt tacttctgca aagcttatgg aggccagccc ggctagcggc
     2041 ccaaggcatc tcatggaccc gcacatcttc accagcaact tcaacaacgg catcggcagg
     2101 cacaagacct acttgtgcta cgaggtggag aggctcgaca acggaacctc cgtgaagatg
     2161 gaccaacaca gggggttcct ccacaaccaa gccaagaacc tcctctgcgg cttctacggc
     2221 aggcacgccg agttgaggtt cctcgacttg gtgccatccc tccaactcga tccagcccaa
     2281 atctaccgcg tgacctggtt catctcctgg tccccatgct tctcctgggg ttgcgccggc
     2341 gaggttcggg ctttcctcca agaaaacacc cacgtccgcc tccgcatttt cgccgccagg
     2401 atctatgatt acgaccctct ctacaaggag gccctccaga tgctgcggga cgccggtgct
     2461 caggtgagta tcatgaccta cgacgagttc aagcactgct gggacacctt cgttgaccac
     2521 cagggctgcc cattccaacc atgggacggt ctggatgaac acagccaagc cttgtccggc
     2581 aggctccggg ccatcctcca aaaccagggg aactccggga gcgagacgcc aggcacctcc
     2641 gagtcggcca ccccagaatc ttccggcgga tcttctggag gatctagcgg ctccgagaca
     2701 ccaggaacat ccgaatccgc tacaccagag tcttctggag gatctagcgg aggatcttcc
     2761 gaggtggagt tctctcacga gtattggatg aggcacgctc ttacacttgc taagagagct
     2821 tgggacgaaa gagaagtgcc agttggcgcc gttcttgtgc ataataatag ggtgatcggc
     2881 gagggttgga atagaccaat tggaaggcat gatccaacag ctcacgcaga gattatggct
     2941 ctcagacaag gcggcctcgt tatgcagaac tacaggctca ttgacgctac actctacgtg
     3001 acactcgaac cttgcgttat gtgcgccgga gctatgattc attctaggat tggcagggtc
     3061 gtgtttggag ctagggacgc taaaacagga gccgccggat ctcttatgga cgtgttgcat
     3121 catccaggca tgaaccatag ggtggagatt acagagggca ttcttgcaga cgagtgcgct
     3181 gctcttcttt ccgatttctt caggatgaga aggcaggaga ttaaggccca gaagaaggct
     3241 cagtcttcta cagatagcgg aggatcttcc ggaggatcta gcggctccga gacaccagga
     3301 acatccgaaa gcgctacacc agaatctagc ggaggctctt ccggaggatc ttctgaagtg
     3361 gagttctccc acgagtattg gatgaggcac gctcttacac ttgctaaaag ggctagggac
     3421 gaaagggaag ttccagttgg agctgttctc gtgctcaata acagggtgat tggcgagggt
     3481 tggaatagag ccattggact ccatgatcca acagctcacg cagagattat ggctcttaga
     3541 caaggcggcc tcgttatgca gaattacaga ctcatcgacg ccacactcta cgttaccttc
     3601 gaaccttgcg ttatgtgcgc cggagctatg atccattcta ggattggcag ggtcgtgttc
     3661 ggcgttagaa acgctaagac aggagctgca ggctctctta tggacgttct tcattaccca
     3721 ggcatgaatc atagagtgga gatcacagaa ggcattcttg cagacgagtg cgcagctctc
     3781 ctttgctatt tcttcaggat gccgaggcaa gttttcaacg ctcagaagaa ggcccagtct
     3841 tctacagatt ccggcggatc ttctggagga tctagcggct ccgagacacc aggaacatcc
     3901 gaatccgcta caccagagtc ttctggagga tctagcggag gatctgacaa gaagtactcg
     3961 atcggcctcg ccattgggac taactctgtt ggctgggccg tgatcaccga cgagtacaag
     4021 gtgccctcaa agaagttcaa ggtcctgggc aacaccgatc ggcattccat caagaagaat
     4081 ctcattggcg ctctcctgtt cgacagcggc gagacggctg aggctacgcg gctcaagcgc
     4141 accgcccgca ggcggtacac gcgcaggaag aatcgcatct gctacctgca ggagattttc
     4201 tccaacgaga tggcgaaggt tgacgattct ttcttccaca ggctggagga gtcattcctc
     4261 gtggaggagg ataagaagca cgagcggcat ccaatcttcg gcaacattgt cgacgaggtt
     4321 gcctaccacg agaagtaccc tacgatctac catctgcgga agaagctcgt ggactccaca
     4381 gataaggcgg acctccgcct gatctacctc gctctggccc acatgattaa gttcaggggc
     4441 catttcctga tcgaggggga tctcaacccg gacaatagcg atgttgacaa gctgttcatc
     4501 cagctcgtgc agacgtacaa ccagctcttc gaggagaacc ccattaatgc gtcaggcgtc
     4561 gacgcgaagg ctatcctgtc cgctaggctc tcgaagtctc ggcgcctcga gaacctgatc
     4621 gcccagctgc cgggcgagaa gaagaacggc ctgttcggga atctcattgc gctcagcctg
     4681 gggctcacgc ccaacttcaa gtcgaatttc gatctcgctg aggacgccaa gctgcagctc
     4741 tccaaggaca catacgacga tgacctggat aacctcctgg cccagatcgg cgatcagtac
     4801 gcggacctgt tcctcgctgc caagaatctg tcggacgcca tcctcctgtc tgatattctc
     4861 agggtgaaca ccgagattac gaaggctccg ctctcagcct ccatgatcaa gcgctacgac
     4921 gagcaccatc aggatctgac cctcctgaag gcgctggtca ggcagcagct ccccgagaag
     4981 tacaaggaga tcttcttcga tcagtcgaag aacggctacg ctgggtacat tgacggcggg
     5041 gcctctcagg aggagttcta caagttcatc aagccgattc tggagaagat ggacggcacg
     5101 gaggagctgc tggtgaagct caatcgcgag gacctcctga ggaagcagcg gacattcgat
     5161 aacggcagca tcccacacca gattcatctc ggggagctgc acgctatcct gaggaggcag
     5221 gaggacttct accctttcct caaggataac cgcgagaaga tcgagaagat tctgactttc
     5281 aggatcccgt actacgtcgg cccactcgct aggggcaact cccgcttcgc ttggatgacc
     5341 cgcaagtcag aggagacgat cacgccgtgg aacttcgagg aggtggtcga caagggcgct
     5401 agcgctcagt cgttcatcga gaggatgacg aatttcgaca agaacctgcc aaatgagaag
     5461 gtgctcccta agcactcgct cctgtacgag tacttcacag tctacaacga gctgactaag
     5521 gtgaagtatg tgaccgaggg catgaggaag ccggctttcc tgtctgggga gcagaagaag
     5581 gccatcgtgg acctcctgtt caagaccaac cggaaggtca cggttaagca gctcaaggag
     5641 gactacttca agaagattga gtgcttcgat tcggtcgaga tctctggcgt tgaggaccgc
     5701 ttcaacgcct ccctggggac ctaccacgat ctcctgaaga tcattaagga taaggacttc
     5761 ctggacaacg aggagaatga ggatatcctc gaggacattg tgctgacact cactctgttc
     5821 gaggaccggg agatgatcga ggagcgcctg aagacttacg cccatctctt cgatgacaag
     5881 gtcatgaagc agctcaagag gaggaggtac accggctggg ggaggctgag caggaagctc
     5941 atcaacggca ttcgggacaa gcagtccggg aagacgatcc tcgacttcct gaagagcgat
     6001 ggcttcgcga accgcaattt catgcagctg attcacgatg acagcctcac attcaaggag
     6061 gatatccaga aggctcaggt gagcggccag ggggactcgc tgcacgagca tatcgcgaac
     6121 ctcgctggct cgccagctat caagaagggg attctgcaga ccgtgaaggt tgtggacgag
     6181 ctggtgaagg tcatgggcag gcacaagcct gagaacatcg tcattgagat ggcccgggag
     6241 aatcagacca cgcagaaggg ccagaagaac tcacgcgaga ggatgaagag gatcgaggag
     6301 ggcattaagg agctggggtc ccagatcctc aaggagcacc cggtggagaa cacgcagctg
     6361 cagaatgaga agctctacct gtactacctc cagaatggcc gcgatatgta tgtggaccag
     6421 gagctggata ttaacaggct cagcgattac gacgtcgatc atatcgttcc acagtcattc
     6481 ctgaaggatg actccattga caacaaggtc ctcaccaggt cggacaagaa ccggggcaag
     6541 tctgataatg ttccttcaga ggaggtcgtt aagaagatga agaactactg gcgccagctc
     6601 ctgaatgcca agctgatcac gcagcggaag ttcgataacc tcacaaaggc tgagaggggc
     6661 gggctctctg agctggacaa ggcgggcttc atcaagaggc agctggtcga gacacggcag
     6721 atcactaagc acgttgcgca gattctcgac tcacggatga acactaagta cgatgagaat
     6781 gacaagctga tccgcgaggt gaaggtcatc accctgaagt caaagctcgt ctccgacttc
     6841 aggaaggatt tccagttcta caaggttcgg gagatcaaca attaccacca tgcccatgac
     6901 gcgtacctga acgcggtggt cggcacagct ctgatcaaga agtacccaaa gctcgagagc
     6961 gagttcgtgt acggggacta caaggtttac gatgtgagga agatgatcgc caagtcggag
     7021 caggagattg gcaaggctac cgccaagtac ttcttctact ctaacattat gaatttcttc
     7081 aagacagaga tcactctggc caatggcgag atccggaagc gccccctcat cgagacgaac
     7141 ggcgagacgg gggagatcgt gtgggacaag ggcagggatt tcgcgaccgt caggaaggtt
     7201 ctctccatgc cacaagtgaa tatcgtcaag aagacagagg tccagactgg cgggttctct
     7261 aaggagtcaa ttctgcctaa gcggaacagc gacaagctca tcgcccgcaa gaaggactgg
     7321 gatccgaaga agtacggcgg gttcgacagc cccactgtgg cctactcggt cctggttgtg
     7381 gcgaaggttg agaagggcaa gtccaagaag ctcaagagcg tgaaggagct gctggggatc
     7441 acgattatgg agcgctccag cttcgagaag aacccgatcg atttcctgga ggcgaagggc
     7501 tacaaggagg tgaagaagga cctgatcatt aagctcccca agtactcact cttcgagctg
     7561 gagaacggca ggaagcggat gctggcttcc gctggcgagc tgcagaaggg gaacgagctg
     7621 gctctgccgt ccaagtatgt gaacttcctc tacctggcct cccactacga gaagctcaag
     7681 ggcagccccg aggacaacga gcagaagcag ctgttcgtcg agcagcacaa gcattacctc
     7741 gacgagatca ttgagcagat ttccgagttc tccaagcgcg tgatcctggc cgacgcgaat
     7801 ctggataagg tcctctccgc gtacaacaag caccgcgaca agccaatcag ggagcaggct
     7861 gagaatatca ttcatctctt caccctgacg aacctcggcg cccctgctgc tttcaagtac
     7921 ttcgacacaa ctatcgatcg caagaggtac acaagcacta aggaggtcct ggacgcgacc
     7981 ctcatccacc agtcgattac cggcctctac gagacgcgca tcgacctgtc tcagctcggg
     8041 ggcgacaagc ggccagcggc gacgaagaag gcggggcagg cgaagaagaa gaaggaattc
     8101 tccggcggca gcaccaacct gtccgacatc atcgagaagg agacgggcaa gcaactcgtg
     8161 atccaggaga gcatcctcat gctgccagag gaggtggagg aggtcatcgg caacaagcca
     8221 gagtccgaca tcctggtgca caccgcctac gacgagtcca ccgacgagaa cgtcatgctc
     8281 ctgaccagcg acgccccaga gtacaagcca tgggccctcg tcatccagga cagcaacggg
     8341 gagaacaaga tcaagatgct gtcggggggg agcccaaaga agaagcggaa ggtgtaggaa
     8401 ttcggtacgc tgaaatcacc agtctctctc tacaaatcta tctctctcta ttttctccat
     8461 aaataatgtg tgagtagttt cccgataagg gaaattaggg ttcttatagg gtttcgctca
     8521 tgtgttgagc atataagaaa cccttagtat gtatttgtat ttgtaaaata cttctatcaa
     8581 taaaatttct aattcctaaa accaaaatcc agtactaaaa tccagatctc ctaaagtccc
     8641 tatagatctt tgtcgtgaat ataaaccaga cacgagacga ctaaacctgg agcccagacg
     8701 ccgttcgaag ctagaagtac cgcttaggca ggaggccgtt agggaaaaga tgctaaggca
     8761 gggttggtta cgttgactcc cccgtaggtt tggtttaaat atgatgaagt ggacggaagg
     8821 aaggaggaag acaaggaagg ataaggttgc aggccctgtg caaggtaaga agatggaaat
     8881 ttgatagagg tacgctacta tacttatact atacgctaag ggaatgcttg tatttatacc
     8941 ctataccccc taataacccc ttatcaattt aagaaataat ccgcataagc ccccgcttaa
     9001 aaattggtat cagagccatg aataggtcta tgaccaaaac tcaagaggat aaaacctcac
     9061 caaaatacga aagagttctt aactctaaag ataaaagatc tttcaagatc aaaactagtt
     9121 ccctcacacc ggagcatgcg atatcctcga gagatctagg cgtaatcatg gtcatagctg
     9181 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     9241 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     9301 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     9361 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     9421 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     9481 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     9541 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     9601 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     9661 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     9721 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt
     9781 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     9841 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     9901 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     9961 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
    10021 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    10081 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    10141 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    10201 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    10261 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    10321 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    10381 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    10441 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    10501 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    10561 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    10621 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    10681 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    10741 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    10801 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    10861 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    10921 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    10981 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    11041 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    11101 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    11161 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    11221 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    11281 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgccagtgc
//
