LOCUS       Exported               11708 bp ds-DNA     circular SYN 27-JUL-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 16, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
COMMENT     NEBuilder_Assembly
FEATURES             Location/Qualifiers
     source          1..11708
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1..243
                     /label=V
     source          244..1946
                     /label=V
     source          1947..8094
                     /label=V
     source          8782..11708
                     /label=V
     promoter        24..2010
                     /label=Ubi promoter
     CDS             2017..2613
                     /codon_start=1
                     /label=A3A
                     /translation="MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTS
                     VKMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSW
                     GCAGEVRAFLQENTHVRLRIFAARIYDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWD
                     TFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN"
     CDS             2614..2661
                     /codon_start=1
                     /label=XTEN linker
                     /translation="SGSETPGTSESATPES"
     CDS             2662..2757
                     /codon_start=1
                     /label=32aa linker
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             2758..3255
                     /codon_start=1
                     /label=ecTadA(wt)
                     /translation="SEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEGWN
                     RPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRVVFG
                     ARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQKKAQS
                     STD"
     CDS             3256..3351
                     /codon_start=1
                     /label=32aa linker
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             3352..3849
                     /codon_start=1
                     /label=ecTadA*(7.10)
                     /translation="SEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWN
                     RAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFG
                     VRNAKTGAAGSLMDVLHYPGMNHRVEITEGILADECAALLCYFFRMPRQVFNAQKKAQS
                     STD"
     CDS             3850..3945
                     /codon_start=1
                     /label=32aa linker
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             3946..8046
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             8047..8094
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8101..8154
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8155..8403
                     /codon_start=1
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8416..8436
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8437..8490
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8491..8739
                     /codon_start=1
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8752..8772
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8830..9006
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(9549..9565)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    9573..9589
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9597..9627)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9642..9663
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9951..10539)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(10710..11570)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11571..11675)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 caagctaatt cgagctcggt acctgacccg gtcgtgcccc tctctagaga taatgagcat
       61 tgcatgtcta agttataaaa aattaccaca tatttttttt gtcacacttg tttgaagtgc
      121 agtttatcta tctttataca tatatttaaa ctttactcta cgaataatat aatctatagt
      181 actacaataa tatcagtgtt ttagagaatc atataaatga acagttagac atggtctaaa
      241 ggacaattga gtattttgac aacaggactc tacagtttta tctttttagt gtgcatgtgt
      301 tctccttttt ttttgcaaat agcttcacct atataatact tcatccattt tattagtaca
      361 tccatttagg gtttagggtt aatggttttt atagactaat ttttttagta catctatttt
      421 attctatttt agcctctaaa ttaagaaaac taaaactcta ttttagtttt tttatttaat
      481 aatttagata taaaatagaa taaaataaag tgactaaaaa ttaaacaaat accctttaag
      541 aaattaaaaa aactaaggaa acatttttct tgtttcgagt agataatgcc agcctgttaa
      601 acgccgtcga cgagtctaac ggacaccaac cagcgaacca gcagcgtcgc gtcgggccaa
      661 gcgaagcaga cggcacggca tctctgtcgc tgcctctgga cccctctcga tcgagagttc
      721 cgctccaccg ttggacttgc tccgctgtcg gcatccagaa attgcgtggc ggagcggcag
      781 acgtgagccg gcacggcagg cggcctcctc ctcctctcac ggcaccggca gctacggggg
      841 attcctttcc caccgctcct tcgctttccc ttcctcgccc gccgtaataa atagacaccc
      901 cctccacacc ctctttcccc aacctcgtgt tgttcggagc gcacacacac acaaccagat
      961 ctcccccaaa tccacccgtc ggcacctccg cttcaaggta cgccgctcgt cctccccccc
     1021 cccccctctc taccttctct agatcggcgt tccggtccat ggttagggcc cggtagttct
     1081 acttctgttc atgtttgtgt tagatccgtg tttgtgttag atccgtgctg ctagcgttcg
     1141 tacacggatg cgacctgtac gtcagacacg ttctgattgc taacttgcca gtgtttctct
     1201 ttggggaatc ctgggatggc tctagccgtt ccgcagacgg gatcgatttc atgatttttt
     1261 ttgtttcgtt gcatagggtt tggtttgccc ttttccttta tttcaatata tgccgtgcac
     1321 ttgtttgtcg ggtcatcttt tcatgctttt ttttgtcttg gttgtgatga tgtggtctgg
     1381 ttgggcggtc gttctagatc ggagtagaat taattctgtt tcaaactacc tggtggattt
     1441 attaattttg gatctgtatg tgtgtgccat acatattcat agttacgaat tgaagatgat
     1501 ggatggaaat atcgatctag gataggtata catgttgatg cgggttttac tgatgcatat
     1561 acagagatgc tttttgttcg cttggttgtg atgatgtggt gtggttgggc ggtcgttcat
     1621 tcgttctaga tcggagtaga atactgtttc aaactacctg gtgtatttat taattttgga
     1681 actgtatgtg tgtgtcatac atcttcatag ttacgagttt aagatggatg gaaatatcga
     1741 tctaggatag gtatacatgt tgatgtgggt tttactgatg catatacatg atggcatatg
     1801 cagcatctat tcatatgctc taaccttgag tacctatcta ttataataaa caagtatgtt
     1861 ttataattat tttgatcttg atatacttgg atgatggcat atgcagcagc tatatgtgga
     1921 tttttttagc cctgccttca tacgctattt atttgcttgg tactgtttct tttgtcgatg
     1981 ctcaccctgt tgtttggtgt tacttctgca aagcttatgg aggccagccc ggctagcggc
     2041 ccaaggcatc tcatggaccc gcacatcttc accagcaact tcaacaacgg catcggcagg
     2101 cacaagacct acttgtgcta cgaggtggag aggctcgaca acggaacctc cgtgaagatg
     2161 gaccaacaca gggggttcct ccacaaccaa gccaagaacc tcctctgcgg cttctacggc
     2221 aggcacgccg agttgaggtt cctcgacttg gtgccatccc tccaactcga tccagcccaa
     2281 atctaccgcg tgacctggtt catctcctgg tccccatgct tctcctgggg ttgcgccggc
     2341 gaggttcggg ctttcctcca agaaaacacc cacgtccgcc tccgcatttt cgccgccagg
     2401 atctatgatt acgaccctct ctacaaggag gccctccaga tgctgcggga cgccggtgct
     2461 caggtgagta tcatgaccta cgacgagttc aagcactgct gggacacctt cgttgaccac
     2521 cagggctgcc cattccaacc atgggacggt ctggatgaac acagccaagc cttgtccggc
     2581 aggctccggg ccatcctcca aaaccagggg aactccggga gcgagacgcc aggcacctcc
     2641 gagtcggcca ccccagaatc ttccggcgga tcttctggag gatctagcgg ctccgagaca
     2701 ccaggaacat ccgaatccgc tacaccagag tcttctggag gatctagcgg aggatcttcc
     2761 gaggtggagt tctctcacga gtattggatg aggcacgctc ttacacttgc taagagagct
     2821 tgggacgaaa gagaagtgcc agttggcgcc gttcttgtgc ataataatag ggtgatcggc
     2881 gagggttgga atagaccaat tggaaggcat gatccaacag ctcacgcaga gattatggct
     2941 ctcagacaag gcggcctcgt tatgcagaac tacaggctca ttgacgctac actctacgtg
     3001 acactcgaac cttgcgttat gtgcgccgga gctatgattc attctaggat tggcagggtc
     3061 gtgtttggag ctagggacgc taaaacagga gccgccggat ctcttatgga cgtgttgcat
     3121 catccaggca tgaaccatag ggtggagatt acagagggca ttcttgcaga cgagtgcgct
     3181 gctcttcttt ccgatttctt caggatgaga aggcaggaga ttaaggccca gaagaaggct
     3241 cagtcttcta cagatagcgg aggatcttcc ggaggatcta gcggctccga gacaccagga
     3301 acatccgaaa gcgctacacc agaatctagc ggaggctctt ccggaggatc ttctgaagtg
     3361 gagttctccc acgagtattg gatgaggcac gctcttacac ttgctaaaag ggctagggac
     3421 gaaagggaag ttccagttgg agctgttctc gtgctcaata acagggtgat tggcgagggt
     3481 tggaatagag ccattggact ccatgatcca acagctcacg cagagattat ggctcttaga
     3541 caaggcggcc tcgttatgca gaattacaga ctcatcgacg ccacactcta cgttaccttc
     3601 gaaccttgcg ttatgtgcgc cggagctatg atccattcta ggattggcag ggtcgtgttc
     3661 ggcgttagaa acgctaagac aggagctgca ggctctctta tggacgttct tcattaccca
     3721 ggcatgaatc atagagtgga gatcacagaa ggcattcttg cagacgagtg cgcagctctc
     3781 ctttgctatt tcttcaggat gccgaggcaa gttttcaacg ctcagaagaa ggcccagtct
     3841 tctacagatt ccggcggatc ttctggagga tctagcggct ccgagacacc aggaacatcc
     3901 gaatccgcta caccagagtc ttctggagga tctagcggag gatctgacaa gaagtactcg
     3961 atcggcctcg ccattgggac taactctgtt ggctgggccg tgatcaccga cgagtacaag
     4021 gtgccctcaa agaagttcaa ggtcctgggc aacaccgatc ggcattccat caagaagaat
     4081 ctcattggcg ctctcctgtt cgacagcggc gagacggctg aggctacgcg gctcaagcgc
     4141 accgcccgca ggcggtacac gcgcaggaag aatcgcatct gctacctgca ggagattttc
     4201 tccaacgaga tggcgaaggt tgacgattct ttcttccaca ggctggagga gtcattcctc
     4261 gtggaggagg ataagaagca cgagcggcat ccaatcttcg gcaacattgt cgacgaggtt
     4321 gcctaccacg agaagtaccc tacgatctac catctgcgga agaagctcgt ggactccaca
     4381 gataaggcgg acctccgcct gatctacctc gctctggccc acatgattaa gttcaggggc
     4441 catttcctga tcgaggggga tctcaacccg gacaatagcg atgttgacaa gctgttcatc
     4501 cagctcgtgc agacgtacaa ccagctcttc gaggagaacc ccattaatgc gtcaggcgtc
     4561 gacgcgaagg ctatcctgtc cgctaggctc tcgaagtctc ggcgcctcga gaacctgatc
     4621 gcccagctgc cgggcgagaa gaagaacggc ctgttcggga atctcattgc gctcagcctg
     4681 gggctcacgc ccaacttcaa gtcgaatttc gatctcgctg aggacgccaa gctgcagctc
     4741 tccaaggaca catacgacga tgacctggat aacctcctgg cccagatcgg cgatcagtac
     4801 gcggacctgt tcctcgctgc caagaatctg tcggacgcca tcctcctgtc tgatattctc
     4861 agggtgaaca ccgagattac gaaggctccg ctctcagcct ccatgatcaa gcgctacgac
     4921 gagcaccatc aggatctgac cctcctgaag gcgctggtca ggcagcagct ccccgagaag
     4981 tacaaggaga tcttcttcga tcagtcgaag aacggctacg ctgggtacat tgacggcggg
     5041 gcctctcagg aggagttcta caagttcatc aagccgattc tggagaagat ggacggcacg
     5101 gaggagctgc tggtgaagct caatcgcgag gacctcctga ggaagcagcg gacattcgat
     5161 aacggcagca tcccacacca gattcatctc ggggagctgc acgctatcct gaggaggcag
     5221 gaggacttct accctttcct caaggataac cgcgagaaga tcgagaagat tctgactttc
     5281 aggatcccgt actacgtcgg cccactcgct aggggcaact cccgcttcgc ttggatgacc
     5341 cgcaagtcag aggagacgat cacgccgtgg aacttcgagg aggtggtcga caagggcgct
     5401 agcgctcagt cgttcatcga gaggatgacg aatttcgaca agaacctgcc aaatgagaag
     5461 gtgctcccta agcactcgct cctgtacgag tacttcacag tctacaacga gctgactaag
     5521 gtgaagtatg tgaccgaggg catgaggaag ccggctttcc tgtctgggga gcagaagaag
     5581 gccatcgtgg acctcctgtt caagaccaac cggaaggtca cggttaagca gctcaaggag
     5641 gactacttca agaagattga gtgcttcgat tcggtcgaga tctctggcgt tgaggaccgc
     5701 ttcaacgcct ccctggggac ctaccacgat ctcctgaaga tcattaagga taaggacttc
     5761 ctggacaacg aggagaatga ggatatcctc gaggacattg tgctgacact cactctgttc
     5821 gaggaccggg agatgatcga ggagcgcctg aagacttacg cccatctctt cgatgacaag
     5881 gtcatgaagc agctcaagag gaggaggtac accggctggg ggaggctgag caggaagctc
     5941 atcaacggca ttcgggacaa gcagtccggg aagacgatcc tcgacttcct gaagagcgat
     6001 ggcttcgcga accgcaattt catgcagctg attcacgatg acagcctcac attcaaggag
     6061 gatatccaga aggctcaggt gagcggccag ggggactcgc tgcacgagca tatcgcgaac
     6121 ctcgctggct cgccagctat caagaagggg attctgcaga ccgtgaaggt tgtggacgag
     6181 ctggtgaagg tcatgggcag gcacaagcct gagaacatcg tcattgagat ggcccgggag
     6241 aatcagacca cgcagaaggg ccagaagaac tcacgcgaga ggatgaagag gatcgaggag
     6301 ggcattaagg agctggggtc ccagatcctc aaggagcacc cggtggagaa cacgcagctg
     6361 cagaatgaga agctctacct gtactacctc cagaatggcc gcgatatgta tgtggaccag
     6421 gagctggata ttaacaggct cagcgattac gacgtcgatc atatcgttcc acagtcattc
     6481 ctgaaggatg actccattga caacaaggtc ctcaccaggt cggacaagaa ccggggcaag
     6541 tctgataatg ttccttcaga ggaggtcgtt aagaagatga agaactactg gcgccagctc
     6601 ctgaatgcca agctgatcac gcagcggaag ttcgataacc tcacaaaggc tgagaggggc
     6661 gggctctctg agctggacaa ggcgggcttc atcaagaggc agctggtcga gacacggcag
     6721 atcactaagc acgttgcgca gattctcgac tcacggatga acactaagta cgatgagaat
     6781 gacaagctga tccgcgaggt gaaggtcatc accctgaagt caaagctcgt ctccgacttc
     6841 aggaaggatt tccagttcta caaggttcgg gagatcaaca attaccacca tgcccatgac
     6901 gcgtacctga acgcggtggt cggcacagct ctgatcaaga agtacccaaa gctcgagagc
     6961 gagttcgtgt acggggacta caaggtttac gatgtgagga agatgatcgc caagtcggag
     7021 caggagattg gcaaggctac cgccaagtac ttcttctact ctaacattat gaatttcttc
     7081 aagacagaga tcactctggc caatggcgag atccggaagc gccccctcat cgagacgaac
     7141 ggcgagacgg gggagatcgt gtgggacaag ggcagggatt tcgcgaccgt caggaaggtt
     7201 ctctccatgc cacaagtgaa tatcgtcaag aagacagagg tccagactgg cgggttctct
     7261 aaggagtcaa ttctgcctaa gcggaacagc gacaagctca tcgcccgcaa gaaggactgg
     7321 gatccgaaga agtacggcgg gttcgacagc cccactgtgg cctactcggt cctggttgtg
     7381 gcgaaggttg agaagggcaa gtccaagaag ctcaagagcg tgaaggagct gctggggatc
     7441 acgattatgg agcgctccag cttcgagaag aacccgatcg atttcctgga ggcgaagggc
     7501 tacaaggagg tgaagaagga cctgatcatt aagctcccca agtactcact cttcgagctg
     7561 gagaacggca ggaagcggat gctggcttcc gctggcgagc tgcagaaggg gaacgagctg
     7621 gctctgccgt ccaagtatgt gaacttcctc tacctggcct cccactacga gaagctcaag
     7681 ggcagccccg aggacaacga gcagaagcag ctgttcgtcg agcagcacaa gcattacctc
     7741 gacgagatca ttgagcagat ttccgagttc tccaagcgcg tgatcctggc cgacgcgaat
     7801 ctggataagg tcctctccgc gtacaacaag caccgcgaca agccaatcag ggagcaggct
     7861 gagaatatca ttcatctctt caccctgacg aacctcggcg cccctgctgc tttcaagtac
     7921 ttcgacacaa ctatcgatcg caagaggtac acaagcacta aggaggtcct ggacgcgacc
     7981 ctcatccacc agtcgattac cggcctctac gagacgcgca tcgacctgtc tcagctcggg
     8041 ggcgacaagc ggccagcggc gacgaagaag gcggggcagg cgaagaagaa gaaggaattc
     8101 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctaccaac
     8161 ctgtccgaca tcatcgagaa ggagacgggc aagcaactcg tgatccagga gagcatcctc
     8221 atgctgccag aggaggtgga ggaggtcatc ggcaacaagc cagagtccga catcctggtg
     8281 cacaccgcct acgacgagtc caccgacgag aacgtcatgc tcctgaccag cgacgcccca
     8341 gagtacaagc catgggccct cgtcatccag gacagcaacg gggagaacaa gatcaagatg
     8401 ctgtcggggg ggagcccaaa gaagaagcgg aaggtggagg gcagaggaag tcttctaaca
     8461 tgcggtgacg tggaggagaa tcccggccct accaacctgt ccgacatcat cgagaaggag
     8521 acgggcaagc aactcgtgat ccaggagagc atcctcatgc tgccagagga ggtggaggag
     8581 gtcatcggca acaagccaga gtccgacatc ctggtgcaca ccgcctacga cgagtccacc
     8641 gacgagaacg tcatgctcct gaccagcgac gccccagagt acaagccatg ggccctcgtc
     8701 atccaggaca gcaacgggga gaacaagatc aagatgctgt cgggggggag cccaaagaag
     8761 aagcggaagg tgtagaccgg tggtacgctg aaatcaccag tctctctcta caaatctatc
     8821 tctctctatt ttctccataa ataatgtgtg agtagtttcc cgataaggga aattagggtt
     8881 cttatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt atttgtattt
     8941 gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag tactaaaatc
     9001 cagatctcct aaagtcccta tagatctttg tcgtgaatat aaaccagaca cgagacgact
     9061 aaacctggag cccagacgcc gttcgaagct agaagtaccg cttaggcagg aggccgttag
     9121 ggaaaagatg ctaaggcagg gttggttacg ttgactcccc cgtaggtttg gtttaaatat
     9181 gatgaagtgg acggaaggaa ggaggaagac aaggaaggat aaggttgcag gccctgtgca
     9241 aggtaagaag atggaaattt gatagaggta cgctactata cttatactat acgctaaggg
     9301 aatgcttgta tttataccct atacccccta ataacccctt atcaatttaa gaaataatcc
     9361 gcataagccc ccgcttaaaa attggtatca gagccatgaa taggtctatg accaaaactc
     9421 aagaggataa aacctcacca aaatacgaaa gagttcttaa ctctaaagat aaaagatctt
     9481 tcaagatcaa aactagttcc ctcacaccgg agcatgcgat atcctcgaga gatctaggcg
     9541 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     9601 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     9661 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     9721 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     9781 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     9841 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     9901 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     9961 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    10021 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    10081 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    10141 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    10201 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    10261 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    10321 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    10381 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    10441 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    10501 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    10561 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    10621 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    10681 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    10741 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    10801 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    10861 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    10921 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    10981 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
    11041 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    11101 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    11161 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    11221 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    11281 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
    11341 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    11401 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    11461 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    11521 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    11581 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    11641 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    11701 gccagtgc
//
