LOCUS       Exported               10349 bp ds-DNA     circular SYN 26-SEP-2018
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 16, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10349
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        13..1999
                     /label=UBI-pro
     CDS             2006..2506
                     /codon_start=1
                     /label=cTadA(wt)
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEGW
                     NRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRVVF
                     GARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQKKAQ
                     SSTD"
     CDS             2507..2602
                     /codon_start=1
                     /label=32aa linker
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             2603..3100
                     /codon_start=1
                     /label=ecTadA*(7.10)
                     /translation="SEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWN
                     RAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFG
                     VRNAKTGAAGSLMDVLHYPGMNHRVEITEGILADECAALLCYFFRMPRQVFNAQKKAQS
                     STD"
     CDS             3101..3196
                     /codon_start=1
                     /label=32aa linker
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             3203..7303
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)-NG
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     misc_feature    6530..6532
                     /label=L1111R
     misc_feature    6602..6604
                     /label=D1135V
     misc_feature    6851..6853
                     /label=G1218R
     misc_feature    6854..6856
                     /label=E1219F
     misc_feature    7163..7165
                     /label=A1322R
     misc_feature    7202..7203
                     /label=R1335V
     misc_feature    7208..7210
                     /label=T1337R
     CDS             7316..7336
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7349..7369
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7382..7402
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7460..7636
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(8179..8195)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8203..8219
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8227..8257)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8272..8293
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8581..9169)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9340..10200)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10201..10305)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagctcggta cctgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
       61 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
      121 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
      181 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
      241 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
      301 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
      361 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
      421 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
      481 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
      541 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
      601 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
      661 ggcacggcat ctctgtcgct gcctctggac ccctctcgat cgagagttcc gctccaccgt
      721 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
      781 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
      841 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
      901 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
      961 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc ccccctctct
     1021 accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta cttctgttca
     1081 tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc
     1141 gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc
     1201 tgggatggct ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg
     1261 catagggttt ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg
     1321 gtcatctttt catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg
     1381 ttctagatcg gagtagaatt aattctgttt caaactacct ggtggattta ttaattttgg
     1441 atctgtatgt gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata
     1501 tcgatctagg ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct
     1561 ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat
     1621 cggagtagaa tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt
     1681 gtgtcataca tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg
     1741 tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt
     1801 catatgctct aaccttgagt acctatctat tataataaac aagtatgttt tataattatt
     1861 ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat ttttttagcc
     1921 ctgccttcat acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt
     1981 gtttggtgtt acttctgcaa agcttatgtc cgaggtggag ttctctcacg agtattggat
     2041 gaggcacgct cttacacttg ctaagagagc ttgggacgaa agagaagtgc cagttggcgc
     2101 cgttcttgtg cataataata gggtgatcgg cgagggttgg aatagaccaa ttggaaggca
     2161 tgatccaaca gctcacgcag agattatggc tctcagacaa ggcggcctcg ttatgcagaa
     2221 ctacaggctc attgacgcta cactctacgt gacactcgaa ccttgcgtta tgtgcgccgg
     2281 agctatgatt cattctagga ttggcagggt cgtgtttgga gctagggacg ctaaaacagg
     2341 agccgccgga tctcttatgg acgtgttgca tcatccaggc atgaaccata gggtggagat
     2401 tacagagggc attcttgcag acgagtgcgc tgctcttctt tccgatttct tcaggatgag
     2461 aaggcaggag attaaggccc agaagaaggc tcagtcttct acagatagcg gaggatcttc
     2521 cggaggatct agcggctccg agacaccagg aacatccgaa agcgctacac cagaatctag
     2581 cggaggctct tccggaggat cttctgaagt ggagttctcc cacgagtatt ggatgaggca
     2641 cgctcttaca cttgctaaaa gggctaggga cgaaagggaa gttccagttg gagctgttct
     2701 cgtgctcaat aacagggtga ttggcgaggg ttggaataga gccattggac tccatgatcc
     2761 aacagctcac gcagagatta tggctcttag acaaggcggc ctcgttatgc agaattacag
     2821 actcatcgac gccacactct acgttacctt cgaaccttgc gttatgtgcg ccggagctat
     2881 gatccattct aggattggca gggtcgtgtt cggcgttaga aacgctaaga caggagctgc
     2941 aggctctctt atggacgttc ttcattaccc aggcatgaat catagagtgg agatcacaga
     3001 aggcattctt gcagacgagt gcgcagctct cctttgctat ttcttcagga tgccgaggca
     3061 agttttcaac gctcagaaga aggcccagtc ttctacagat tccggcggat cttctggagg
     3121 atctagcggc tccgagacac caggaacatc cgaatccgct acaccagagt cttctggagg
     3181 atctagcgga ggatctctta aggacaagaa gtactcgatc ggcctcgcca ttgggactaa
     3241 ctctgttggc tgggccgtga tcaccgacga gtacaaggtg ccctcaaaga agttcaaggt
     3301 cctgggcaac accgatcggc attccatcaa gaagaatctc attggcgctc tcctgttcga
     3361 cagcggcgag acggctgagg ctacgcggct caagcgcacc gcccgcaggc ggtacacgcg
     3421 caggaagaat cgcatctgct acctgcagga gattttctcc aacgagatgg cgaaggttga
     3481 cgattctttc ttccacaggc tggaggagtc attcctcgtg gaggaggata agaagcacga
     3541 gcggcatcca atcttcggca acattgtcga cgaggttgcc taccacgaga agtaccctac
     3601 gatctaccat ctgcggaaga agctcgtgga ctccacagat aaggcggacc tccgcctgat
     3661 ctacctcgct ctggcccaca tgattaagtt caggggccat ttcctgatcg agggggatct
     3721 caacccggac aatagcgatg ttgacaagct gttcatccag ctcgtgcaga cgtacaacca
     3781 gctcttcgag gagaacccca ttaatgcgtc aggcgtcgac gcgaaggcta tcctgtccgc
     3841 taggctctcg aagtctcggc gcctcgagaa cctgatcgcc cagctgccgg gcgagaagaa
     3901 gaacggcctg ttcgggaatc tcattgcgct cagcctgggg ctcacgccca acttcaagtc
     3961 gaatttcgat ctcgctgagg acgccaagct gcagctctcc aaggacacat acgacgatga
     4021 cctggataac ctcctggccc agatcggcga tcagtacgcg gacctgttcc tcgctgccaa
     4081 gaatctgtcg gacgccatcc tcctgtctga tattctcagg gtgaacaccg agattacgaa
     4141 ggctccgctc tcagcctcca tgatcaagcg ctacgacgag caccatcagg atctgaccct
     4201 cctgaaggcg ctggtcaggc agcagctccc cgagaagtac aaggagatct tcttcgatca
     4261 gtcgaagaac ggctacgctg ggtacattga cggcggggcc tctcaggagg agttctacaa
     4321 gttcatcaag ccgattctgg agaagatgga cggcacggag gagctgctgg tgaagctcaa
     4381 tcgcgaggac ctcctgagga agcagcggac attcgataac ggcagcatcc cacaccagat
     4441 tcatctcggg gagctgcacg ctatcctgag gaggcaggag gacttctacc ctttcctcaa
     4501 ggataaccgc gagaagatcg agaagattct gactttcagg atcccgtact acgtcggccc
     4561 actcgctagg ggcaactccc gcttcgcttg gatgacccgc aagtcagagg agacgatcac
     4621 gccgtggaac ttcgaggagg tggtcgacaa gggcgctagc gctcagtcgt tcatcgagag
     4681 gatgacgaat ttcgacaaga acctgccaaa tgagaaggtg ctccctaagc actcgctcct
     4741 gtacgagtac ttcacagtct acaacgagct gactaaggtg aagtatgtga ccgagggcat
     4801 gaggaagccg gctttcctgt ctggggagca gaagaaggcc atcgtggacc tcctgttcaa
     4861 gaccaaccgg aaggtcacgg ttaagcagct caaggaggac tacttcaaga agattgagtg
     4921 cttcgattcg gtcgagatct ctggcgttga ggaccgcttc aacgcctccc tggggaccta
     4981 ccacgatctc ctgaagatca ttaaggataa ggacttcctg gacaacgagg agaatgagga
     5041 tatcctcgag gacattgtgc tgacactcac tctgttcgag gaccgggaga tgatcgagga
     5101 gcgcctgaag acttacgccc atctcttcga tgacaaggtc atgaagcagc tcaagaggag
     5161 gaggtacacc ggctggggga ggctgagcag gaagctcatc aacggcattc gggacaagca
     5221 gtccgggaag acgatcctcg acttcctgaa gagcgatggc ttcgcgaacc gcaatttcat
     5281 gcagctgatt cacgatgaca gcctcacatt caaggaggat atccagaagg ctcaggtgag
     5341 cggccagggg gactcgctgc acgagcatat cgcgaacctc gctggctcgc cagctatcaa
     5401 gaaggggatt ctgcagaccg tgaaggttgt ggacgagctg gtgaaggtca tgggcaggca
     5461 caagcctgag aacatcgtca ttgagatggc ccgggagaat cagaccacgc agaagggcca
     5521 gaagaactca cgcgagagga tgaagaggat cgaggagggc attaaggagc tggggtccca
     5581 gatcctcaag gagcacccgg tggagaacac gcagctgcag aatgagaagc tctacctgta
     5641 ctacctccag aatggccgcg atatgtatgt ggaccaggag ctggatatta acaggctcag
     5701 cgattacgac gtcgatcata tcgttccaca gtcattcctg aaggatgact ccattgacaa
     5761 caaggtcctc accaggtcgg acaagaaccg gggcaagtct gataatgttc cttcagagga
     5821 ggtcgttaag aagatgaaga actactggcg ccagctcctg aatgccaagc tgatcacgca
     5881 gcggaagttc gataacctca caaaggctga gaggggcggg ctctctgagc tggacaaggc
     5941 gggcttcatc aagaggcagc tggtcgagac acggcagatc actaagcacg ttgcgcagat
     6001 tctcgactca cggatgaaca ctaagtacga tgagaatgac aagctgatcc gcgaggtgaa
     6061 ggtcatcacc ctgaagtcaa agctcgtctc cgacttcagg aaggatttcc agttctacaa
     6121 ggttcgggag atcaacaatt accaccatgc ccatgacgcg tacctgaacg cggtggtcgg
     6181 cacagctctg atcaagaagt acccaaagct cgagagcgag ttcgtgtacg gggactacaa
     6241 ggtttacgat gtgaggaaga tgatcgccaa gtcggagcag gagattggca aggctaccgc
     6301 caagtacttc ttctactcta acattatgaa tttcttcaag acagagatca ctctggccaa
     6361 tggcgagatc cggaagcgcc ccctcatcga gacgaacggc gagacggggg agatcgtgtg
     6421 ggacaagggc agggatttcg cgaccgtcag gaaggttctc tccatgccac aagtgaatat
     6481 cgtcaagaag acagaggtcc agactggcgg gttctctaag gagtcaattc ggcctaagcg
     6541 gaacagcgac aagctcatcg cccgcaagaa ggactgggat ccgaagaagt acggcgggtt
     6601 cgtcagcccc actgtggcct actcggtcct ggttgtggcg aaggttgaga agggcaagtc
     6661 caagaagctc aagagcgtga aggagctgct ggggatcacg attatggagc gctccagctt
     6721 cgagaagaac ccgatcgatt tcctggaggc gaagggctac aaggaggtga agaaggacct
     6781 gatcattaag ctccccaagt actcactctt cgagctggag aacggcagga agcggatgct
     6841 ggcttccgct cgcttcctgc agaaggggaa cgagctggct ctgccgtcca agtatgtgaa
     6901 cttcctctac ctggcctccc actacgagaa gctcaagggc agccccgagg acaacgagca
     6961 gaagcagctg ttcgtcgagc agcacaagca ttacctcgac gagatcattg agcagatttc
     7021 cgagttctcc aagcgcgtga tcctggccga cgcgaatctg gataaggtcc tctccgcgta
     7081 caacaagcac cgcgacaagc caatcaggga gcaggctgag aatatcattc atctcttcac
     7141 cctgacgaac ctcggcgccc ctcgtgcttt caagtacttc gacacaacta tcgatcgcaa
     7201 ggtatacaga agcactaagg aggtcctgga cgcgaccctc atccaccagt cgattaccgg
     7261 cctctacgag acgcgcatcg acctgtctca gctcgggggc gactccggcg gcagcccaaa
     7321 gaagaagcgg aaggtgtctg gaggttctcc taagaaaaag agaaaagtgt ccggcggctc
     7381 cccgaagaag aagcgcaagg tgtaggaatt cggtacgctg aaatcaccag tctctctcta
     7441 caaatctatc tctctctatt ttctccataa ataatgtgtg agtagtttcc cgataaggga
     7501 aattagggtt cttatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt
     7561 atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag
     7621 tactaaaatc cagatctcct aaagtcccta tagatctttg tcgtgaatat aaaccagaca
     7681 cgagacgact aaacctggag cccagacgcc gttcgaagct agaagtaccg cttaggcagg
     7741 aggccgttag ggaaaagatg ctaaggcagg gttggttacg ttgactcccc cgtaggtttg
     7801 gtttaaatat gatgaagtgg acggaaggaa ggaggaagac aaggaaggat aaggttgcag
     7861 gccctgtgca aggtaagaag atggaaattt gatagaggta cgctactata cttatactat
     7921 acgctaaggg aatgcttgta tttataccct atacccccta ataacccctt atcaatttaa
     7981 gaaataatcc gcataagccc ccgcttaaaa attggtatca gagccatgaa taggtctatg
     8041 accaaaactc aagaggataa aacctcacca aaatacgaaa gagttcttaa ctctaaagat
     8101 aaaagatctt tcaagatcaa aactagttcc ctcacaccgg agcatgcgat atcctcgaga
     8161 gatctaggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     8221 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     8281 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     8341 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     8401 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     8461 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     8521 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     8581 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     8641 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     8701 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     8761 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     8821 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     8881 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     8941 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     9001 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     9061 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     9121 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     9181 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     9241 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     9301 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     9361 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     9421 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     9481 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     9541 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     9601 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     9661 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     9721 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     9781 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     9841 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     9901 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     9961 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    10021 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    10081 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    10141 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    10201 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    10261 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    10321 agtgccacct gccagtgcca agctaattc
//
