LOCUS pLCR_34_screen_DR1_-_pF 8714 bp ds-DNA circular 06-DEC-2019 DEFINITION . FEATURES Location/Qualifiers misc_binding 3806..4394 /label="WPRE" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 misc_feature complement(2897..2902) /label="BsmBI" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 1681..1922 /label="RRE" /ApEinfo_revcolor=#d6b295 /ApEinfo_fwdcolor=#d6b295 misc_feature 1067..1204 /label="Psi" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd rep_origin 7149..7374 /label="ColE1" /ApEinfo_revcolor=#9eafd2 /ApEinfo_fwdcolor=#9eafd2 CDS 3191..3790 /label="Puro" /ApEinfo_revcolor=#b7e6d7 /ApEinfo_fwdcolor=#b7e6d7 misc_feature 1171..1535 /label="gag" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd LTR 828..1016 /label="5' LTR" /ApEinfo_revcolor=#f8d3a9 /ApEinfo_fwdcolor=#f8d3a9 misc_feature 2904..2908 /label="U6 terminator" /ApEinfo_revcolor=#b1ff67 /ApEinfo_fwdcolor=#b1ff67 promoter 2921..3176 /label="EFS-NS" /ApEinfo_revcolor=#c6c9d1 /ApEinfo_fwdcolor=#c6c9d1 misc_feature 2859..2881 /label="RfxCas13d-DR" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 misc_feature 2889..2894 /label="BsmBI" /ApEinfo_revcolor=#c7b0e3 /ApEinfo_fwdcolor=#c7b0e3 LTR 4465..4700 /label="3' LTR SIN" /ApEinfo_revcolor=#75c6a9 /ApEinfo_fwdcolor=#75c6a9 promoter 2608..2856 /label="U6 promoter" /ApEinfo_revcolor=#f58a5e /ApEinfo_fwdcolor=#f58a5e misc_feature 2440..2557 /label="CPPT; central polypurine tract" /ApEinfo_revcolor=#ff9ccd /ApEinfo_fwdcolor=#ff9ccd ORIGIN 1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg 2641 catatacgat acaaggctgt tagagagata attggaatta atttgactgt aaacacaaag 2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta 2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc 2821 ttggctttat atatcttgtg gaaaggacga aacaccgtac ccctaccaac tggtcggggt 2881 ttgaaacgGA GACGgaCGTC TCttttttGA ATTCGCTAGC TAGGTCTTGA AAGGAGTGgg 2941 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt 3001 ggggggaggg gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga 3061 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag 3121 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggaccg 3181 gttctagagc ATGACCGAGT ACAAGCCCAC GGTGCGCCTC GCCACCCGCG ACGACGTCCC 3241 CAGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT 3301 CGATCCGGAC CGCCACATCG AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT 3361 CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC GGACGACGGC GCCGCGGTGG CGGTCTGGAC 3421 CACGCCGGAG AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC GCATGGCCGA 3481 GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG 3541 GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC CGTCGGAGTC TCGCCCGACC ACCAGGGCAA 3601 GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC 3661 CGCCTTCCTG GAGACCTCCG CGCCCCGCAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC 3721 CGTCACCGCC GACGTCGAGG TGCCCGAAGG ACCGCGCACC TGGTGCATGA CCCGCAAGCC 3781 CGGTGCCTGA ACGCGTTAAG TCGACAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT 3841 GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC 3901 TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG 3961 GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC 4021 TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC 4081 CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC 4141 CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA 4201 ATCATCGTCC TTTCCTTGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC 4261 CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC 4321 GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG 4381 GGCCGCCTCC CCGCGTCGAC TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC 4441 CACTTTTTAA AAGAAAAGGG GGGACTGGAA GGGCTAATTC ACTCCCAACG AAGACAAGAT 4501 CTGCTTTTTG CTTGTACTGG GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT 4561 GGCTAACTAG GGAACCCACT GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA 4621 GTGTGTGCCC GTCTGTTGTG TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA 4681 GTGTGGAAAA TCTCTAGCAG ggcccgttta aacccgctga tcagcctcga ctgtgccttc 4741 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc 4801 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg 4861 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa 4921 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg 4981 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 5041 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 5101 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 5161 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 5221 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 5281 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 5341 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 5401 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt 5461 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 5521 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat 5581 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 5641 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc 5701 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 5761 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg 5821 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact 5881 aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag 5941 cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg 6001 ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc 6061 cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt 6121 cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg 6181 agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg 6241 tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg 6301 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg 6361 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca 6421 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 6481 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct 6541 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 6601 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 6661 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 6721 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 6781 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 6841 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 6901 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 6961 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 7021 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 7081 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 7141 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 7201 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 7261 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 7321 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 7381 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 7441 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 7501 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 7561 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 7621 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 7681 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 7741 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 7801 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 7861 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 7921 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 7981 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 8041 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 8101 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 8161 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 8221 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 8281 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 8341 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 8401 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 8461 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 8521 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 8581 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 8641 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 8701 aagtgccacc tgac //