LOCUS       Exported                8961 bp ds-DNA     circular UNA 13-DEC-2019
DEFINITION  pCMV_GSBPNLS_GSAPOBEC_GSCas9n_2xGSUGI_GSBPNLS.
ACCESSION   urn.local...2o-bba1xyq
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 16, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8961
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     promoter        275..356
                     /label=CMV prom
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=CMV prom"
                     /note="/Transferred_Similarity=97.56%"
     promoter        377..395
                     /label=T7 prom
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=T7 prom"
                     /note="/Transferred_Similarity=100.00%"
     CDS             409..465
                     /label=BP NLS
                     /note="/created_by=jdoman"
     CDS             466..1149
                     /label=rAPOBEC1
                     /note="/created_by=jonlevy"
                     /note="/modified_by=jdoman"
     modified_base   838..840
                     /label=R126E
                     /note="/created_by=jdoman"
     misc_feature    838..839
                     /label=AG
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=AG"
     modified_base   856..858
                     /label=R132E
                     /note="/created_by=jdoman"
     misc_feature    856..858
                     /label=CGC
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=CGC"
     CDS             1150..1245
                     /label=BE4 linker
                     /note="/Transferred_From=JL545-pCMV_BPNLS_rAPOBEC1(genscrip
                     t)_dl
                     BE4_NpuN1"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=jonlevy"
                     /note="/modified_by=jdoman"
     CDS             1246..5346
                     /label=SpCas9
                     /note="hSpCas9"
                     /note="/modified_by=jdoman"
     modified_base   1270..1272
                     /label=D10A
                     /note="/created_by=jdoman"
     misc_feature    1536..1546
                     /label=PflMI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CCANNNN^NTGG"
                     /note="/annotation_group=CCANNNNNTGG"
     misc_feature    3717..3727
                     /label=PflMI
                     /note="Geneious type: restriction site"
                     /note="/Recognition_pattern=CCANNNN^NTGG"
                     /note="/annotation_group=CCANNNNNTGG"
     CDS             5347..5376
                     /label=Linker
                     /note="/created_by=jdoman"
                     /note="/modified_by=jdoman"
     CDS             5377..5916
                     /label=2xUGI
                     /note="/created_by=jonlevy"
                     /note="/modified_by=jdoman"
     CDS             5917..5967
                     /label=BP NLS
                     /note="/created_by=jdoman"
                     /note="/modified_by=jdoman"
     terminator      6019..6246
                     /label=bGH_PA term
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=bGH_PA term"
                     /note="/Transferred_Similarity=100.00%"
     promoter        complement(6366..6395)
                     /label=lac prom
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=lac prom"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     complement(6410..6434)
                     /label=ACK32
     misc_feature    complement(6704..7323)
                     /label=pBR322 origin
                     /note="Geneious type: Origin of Replication"
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=pBR322 origin"
                     /note="/Transferred_Similarity=99.52%"
     primer_bind     6719..6743
                     /label=ACK10
     misc_feature    6913^6914
                     /label=A
                     /note="Geneious type: Editing History Deletion"
                     /note="/Original_Bases=A"
     misc_feature    6915
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     primer_bind     6942..6960
                     /label=QB0766 RepO-F
     primer_bind     7039..7058
                     /label=833_BR322-rev1
     primer_bind     complement(7067..7086)
                     /label=QB0767 RepO-R
     misc_feature    7160
                     /label=G
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=G"
     primer_bind     7220..7244
                     /label=ACK11
     primer_bind     complement(7220..7239)
                     /label=832_BR322-fwd1
     misc_feature    complement(7478..8338)
                     /label=amp marker
                     /label=nonstandard type:  Marker
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=amp marker"
                     /note="/Transferred_Similarity=99.88%"
     primer_bind     7719..7743
                     /label=ACK12
     primer_bind     7884..7903
                     /label=ACK563
     primer_bind     8219..8243
                     /label=ACK13
     promoter        complement(8380..8408)
                     /label=amp prom
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=amp prom"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     8452..8469
                     /label=QB0090 pQE promoter
     primer_bind     complement(8522..8541)
                     /label=QB0092 pRS-marker
     primer_bind     8722..8746
                     /label=ACK14
     promoter        join(8729..8961,1..356)
                     /label=CMV_Full
                     /note="/Transferred_From=CMV_Full"
                     /note="/Transferred_Similarity=99.83%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     primer_bind     complement(8779..8798)
                     /label=QB1110 pSicoR
     misc_feature    8918
                     /label=C
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=C"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctcctc agagactggg
      481 cctgtcgccg tcgatccaac cctgcgccgc cggattgaac ctcacgagtt tgaagtgttc
      541 tttgaccccc gggagctgag aaaggagaca tgcctgctgt acgagatcaa ctggggaggc
      601 aggcactcca tctggaggca cacctctcag aacacaaata agcacgtgga ggtgaacttc
      661 atcgagaagt ttaccacaga gcggtacttc tgccccaata ccagatgtag catcacatgg
      721 tttctgagct ggtccccttg cggagagtgt agcagggcca tcaccgagtt cctgtccaga
      781 tatccacacg tgacactgtt tatctacatc gccaggctgt atcaccacgc agacccagag
      841 aataggcagg gcctggagga tctgatcagc tccggcgtga ccatccagat catgacagag
      901 caggagtccg gctactgctg gcggaacttc gtgaattatt ctcctagcaa cgaggcccac
      961 tggcctaggt acccacacct gtgggtgcgc ctgtacgtgc tggagctgta ttgcatcatc
     1021 ctgggcctgc ccccttgtct gaatatcctg cggagaaagc agccccagct gaccttcttt
     1081 acaatcgccc tgcagtcttg tcactatcag aggctgccac cccacatcct gtgggccaca
     1141 ggcctgaagt ctggaggatc tagcggagga tcctctggca gcgagacacc aggaacaagc
     1201 gagtcagcaa caccagagag cagtggcggc agcagcggcg gcagcgacaa gaagtacagc
     1261 atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
     1321 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
     1381 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg gctgaagaga
     1441 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
     1501 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
     1561 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
     1621 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
     1681 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
     1741 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
     1801 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
     1861 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
     1921 gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg
     1981 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
     2041 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac
     2101 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg
     2161 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac
     2221 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag
     2281 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga
     2341 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc
     2401 gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac
     2461 aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag
     2521 gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc
     2581 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc
     2641 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct
     2701 tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag
     2761 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa
     2821 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag
     2881 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag
     2941 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg
     3001 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc
     3061 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt
     3121 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa
     3181 gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg
     3241 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac
     3301 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag
     3361 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat
     3421 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag
     3481 ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag
     3541 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag
     3601 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg
     3661 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag
     3721 gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt
     3781 ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag
     3841 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg
     3901 ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc
     3961 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag
     4021 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat
     4081 gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
     4141 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
     4201 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc
     4261 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
     4321 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
     4381 aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac
     4441 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg
     4501 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
     4561 aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg
     4621 gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt gctggtggtg
     4681 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
     4741 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
     4801 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
     4861 gaaaacggcc ggaagagaat gctggcctct gccggcgaac tgcagaaggg aaacgaactg
     4921 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
     4981 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg
     5041 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat
     5101 ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc
     5161 gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc cttcaagtac
     5221 tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct ggacgccacc
     5281 ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga
     5341 ggtgacagcg gcgggagcgg cgggagcggg gggagcacta atctgagcga catcattgag
     5401 aaggagactg ggaaacagct ggtcattcag gagtccatcc tgatgctgcc tgaggaggtg
     5461 gaggaagtga tcggcaacaa gccagagtct gacatcctgg tgcacaccgc ctacgacgag
     5521 tccacagatg agaatgtgat gctgctgacc tctgacgccc ccgagtataa gccttgggcc
     5581 ctggtcatcc aggattctaa cggcgagaat aagatcaaga tgctgagcgg aggatccgga
     5641 ggatctggag gcagcaccaa cctgtctgac atcatcgaga aggagacagg caagcagctg
     5701 gtcatccagg agagcatcct gatgctgccc gaagaagtcg aagaagtgat cggaaacaag
     5761 cctgagagcg atatcctggt ccataccgcc tacgacgaga gtaccgacga aaatgtgatg
     5821 ctgctgacat ccgacgcccc agagtataag ccctgggctc tggtcatcca ggattccaac
     5881 ggagagaaca aaatcaaaat gctgtctggc ggctcaaaaa gaaccgccga cggcagcgaa
     5941 ttcgagccca agaagaagag gaaagtctaa ccggtcatca tcaccatcac cattgagttt
     6001 aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     6061 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     6121 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     6181 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
     6241 ctatggcttc tgaggcggaa agaaccagct ggggctcgat accgtcgacc tctagctaga
     6301 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     6361 cacacaacat acgagccgga agcataaagt gtaaagccta gggtgcctaa tgagtgagct
     6421 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     6481 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     6541 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     6601 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     6661 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     6721 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     6781 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     6841 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     6901 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     6961 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     7021 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     7081 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     7141 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     7201 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     7261 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     7321 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     7381 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     7441 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     7501 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     7561 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     7621 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     7681 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     7741 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     7801 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     7861 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     7921 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     7981 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     8041 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     8101 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     8161 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     8221 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     8281 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     8341 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     8401 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     8461 tgccacctga cgtcgacgga tcgggagatc gatctcccga tcccctaggg tcgactctca
     8521 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
     8581 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
     8641 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
     8701 gatatacgcg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     8761 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     8821 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     8881 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     8941 tggcagtaca tcaagtgtat c
//
