LOCUS       Exported                9024 bp ds-DNA     circular UNA 13-DEC-2019
DEFINITION  [pBR322] pCMV bpNLS YE1-CP1028-CBEmax bpNLS bla.
ACCESSION   urn.local...10-bb99n4s
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 16, 2019 from SnapGene Viewer 5.0.6
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9024
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     source          1246..5409
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label=source synthetic DNA construct
     promoter        275..356
                     /label=CMV prom
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=CMV prom"
                     /note="/Transferred_Similarity=97.56%"
     promoter        377..395
                     /label=T7 prom
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=T7 prom"
                     /note="/Transferred_Similarity=100.00%"
     CDS             409..465
                     /label=BP NLS
                     /note="/created_by=jdoman"
     CDS             466..1149
                     /label=rAPOBEC1
                     /note="/created_by=jonlevy"
                     /note="/modified_by=jdoman"
     misc_feature    730..732
                     /label=W90Y
                     /label=nonstandard type:  Polymorphism
                     /note="/created_by=User"
     misc_feature    731..732
                     /label=GG
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=GG"
     misc_feature    838..840
                     /label=R126E
                     /label=nonstandard type:  Polymorphism
                     /note="/created_by=User"
     misc_feature    838..839
                     /label=AG
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=AG"
     CDS             1150..1245
                     /label=BE4 linker
                     /note="/Transferred_From=JL545-pCMV_BPNLS_rAPOBEC1(genscrip
                     t)_dl
                     BE4_NpuN1"
                     /note="/Transferred_Similarity=100.00%"
                     /note="/created_by=jonlevy"
                     /note="/modified_by=jdoman"
     CDS             1246..5409
                     /label=SpCas9-CP1028
                     /note="/created_by=kzhao"
                     /note="/modified_by=jdoman"
     primer_bind     complement(1543..1565)
                     /label=MA263
     primer_bind     1553..1589
                     /label=MA264
     modified_base   1567..1569
                     /label=D1135V
                     /note="/created_by=kzhao"
     primer_bind     complement(1791..1812)
                     /label=MA265
     primer_bind     1801..1843
                     /label=MA266
     modified_base   1816..1818
                     /label=G1218R
                     /note="/created_by=kzhao"
     CDS             1909..1911
                     /label=Newstrt-15
     CDS             2008..2010
                     /label=Newstrt-16
     primer_bind     complement(2142..2179)
                     /label=MA267
     primer_bind     2150..2168
                     /label=AR318
     modified_base   2167..2169
                     /label=R1335Q
                     /note="/created_by=kzhao"
     modified_base   2173..2175
                     /label=T1337R
                     /note="/created_by=kzhao"
     primer_bind     2176..2197
                     /label=MA262
     misc_feature    2923..2925
                     /label=Cpstrt-2
     CDS             3016..3018
                     /label=CPsrt3
     CDS             5356..5358
                     /label=Newstrt-6
     CDS             5410..5439
                     /label=Linker
                     /note="/created_by=jdoman"
     CDS             5440..5979
                     /label=2xUGI
                     /note="/created_by=kzhao"
                     /note="/modified_by=jdoman"
     CDS             5980..6030
                     /label=BP NLS
                     /note="/created_by=kzhao"
                     /note="/modified_by=jdoman"
     primer_bind     complement(6079..6096)
                     /label=Genewiz BGHR
     terminator      6082..6309
                     /label=bGH_PA term
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=bGH_PA term"
                     /note="/Transferred_Similarity=100.00%"
     primer_bind     complement(6393..6416)
                     /label=QB0108 Tac promoter
     promoter        complement(6429..6458)
                     /label=lac prom
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=lac prom"
                     /note="/Transferred_Similarity=100.00%"
     misc_feature    complement(6767..7386)
                     /label=pBR322 origin
                     /note="Geneious type: Origin of Replication"
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=pBR322 origin"
                     /note="/Transferred_Similarity=99.52%"
     primer_bind     6782..6806
                     /label=ACK10
     primer_bind     complement(6843..6862)
                     /label=BR322-F
     misc_feature    6976^6977
                     /label=A
                     /note="Geneious type: Editing History Deletion"
                     /note="/Original_Bases=A"
     misc_feature    6978
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
                     /note="/Original_Bases="
     primer_bind     7005..7023
                     /label=QB0766 RepO-F
     primer_bind     7102..7121
                     /label=833_BR322-rev1
     primer_bind     complement(7130..7149)
                     /label=QB0767 RepO-R
     misc_feature    7223
                     /label=G
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=G"
     primer_bind     7283..7307
                     /label=ACK11
     primer_bind     complement(7283..7302)
                     /label=832_BR322-fwd1
     misc_feature    complement(7541..8401)
                     /label=amp marker
                     /label=nonstandard type:  Marker
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=amp marker"
                     /note="/Transferred_Similarity=99.88%"
     primer_bind     7782..7806
                     /label=ACK12
     primer_bind     7947..7966
                     /label=ACK563
     primer_bind     8164..8183
                     /label=QB0005 Amp-R
     primer_bind     8249..8276
                     /label=563_Amp_Rev
     primer_bind     complement(8249..8276)
                     /label=562_Amp_Fwd
     primer_bind     8282..8306
                     /label=ACK13
     promoter        complement(8443..8471)
                     /label=amp prom
                     /note="/Imported_from=Pla sMapper"
                     /note="/Transferred_From=amp prom"
                     /note="/Transferred_Similarity=100.00%"
     promoter        join(8792..9024,1..356)
                     /label=CMV_Full
                     /note="/Transferred_From=CMV_Full"
                     /note="/Transferred_Similarity=99.83%"
                     /note="/created_by=User"
                     /note="/modified_by=User"
     misc_feature    8981
                     /label=C
                     /note="Geneious type: Editing History Replacement"
                     /note="/Original_Bases=C"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctcctc agagactggg
      481 cctgtcgccg tcgatccaac cctgcgccgc cggattgaac ctcacgagtt tgaagtgttc
      541 tttgaccccc gggagctgag aaaggagaca tgcctgctgt acgagatcaa ctggggaggc
      601 aggcactcca tctggaggca cacctctcag aacacaaata agcacgtgga ggtgaacttc
      661 atcgagaagt ttaccacaga gcggtacttc tgccccaata ccagatgtag catcacatgg
      721 tttctgagct attccccttg cggagagtgt agcagggcca tcaccgagtt cctgtccaga
      781 tatccacacg tgacactgtt tatctacatc gccaggctgt atcaccacgc agacccagag
      841 aataggcagg gcctgcgcga tctgatcagc tccggcgtga ccatccagat catgacagag
      901 caggagtccg gctactgctg gcggaacttc gtgaattatt ctcctagcaa cgaggcccac
      961 tggcctaggt acccacacct gtgggtgcgc ctgtacgtgc tggagctgta ttgcatcatc
     1021 ctgggcctgc ccccttgtct gaatatcctg cggagaaagc agccccagct gaccttcttt
     1081 acaatcgccc tgcagtcttg tcactatcag aggctgccac cccacatcct gtgggccaca
     1141 ggcctgaagt ctggaggatc tagcggagga tcctctggca gcgagacacc aggaacaagc
     1201 gagtcagcaa caccagagag cagtggcggc agcagcggcg gcagcgaaat cggcaaggct
     1261 accgccaagt acttcttcta cagcaacatc atgaactttt tcaagaccga gattaccctg
     1321 gccaacggcg agatccggaa gcggcctctg atcgagacaa acggcgaaac cggggagatc
     1381 gtgtgggata agggccggga ttttgccacc gtgcggaaag tgctgagcat gccccaagtg
     1441 aatatcgtga aaaagaccga ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc
     1501 aagaggaaca gcgataagct gatcgccaga aagaaggact gggaccctaa gaagtacggc
     1561 ggcttcgaca gccccaccgt ggcctattct gtgctggtgg tggccaaagt ggaaaagggc
     1621 aagtccaaga aactgaagag tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc
     1681 agcttcgaga agaatcccat cgactttctg gaagccaagg gctacaaaga agtgaaaaag
     1741 gacctgatca tcaagctgcc taagtactcc ctgttcgagc tggaaaacgg ccggaagaga
     1801 atgctggcct ctgccggcga actgcagaag ggaaacgaac tggccctgcc ctccaaatat
     1861 gtgaacttcc tgtacctggc cagccactat gagaagctga agggctcccc cgaggataat
     1921 gagcagaaac agctgtttgt ggaacagcac aagcactacc tggacgagat catcgagcag
     1981 atcagcgagt tctccaagag agtgatcctg gccgacgcta atctggacaa agtgctgtcc
     2041 gcctacaaca agcaccggga taagcccatc agagagcagg ccgagaatat catccacctg
     2101 tttaccctga ccaatctggg agcccctgcc gccttcaagt actttgacac caccatcgac
     2161 cggaagaggt acaccagcac caaagaggtg ctggacgcca ccctgatcca ccagagcatc
     2221 accggcctgt acgagacacg gatcgacctg tctcagctgg gaggcgacgg cggatcagga
     2281 ggctctggcg gttcaggtgg atcaggcggt agcggaggtt caggtggtat ggacaagaag
     2341 tacagcatcg gcctggccat cggcaccaac tctgtgggct gggccgtgat caccgacgag
     2401 tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag
     2461 aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg
     2521 aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag
     2581 atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc
     2641 ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac
     2701 gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac
     2761 agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc
     2821 cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg
     2881 ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc
     2941 ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat
     3001 ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg
     3061 agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg
     3121 cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac
     3181 cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac
     3241 atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga
     3301 tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct
     3361 gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac
     3421 ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac
     3481 ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc
     3541 ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg
     3601 cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg
     3661 accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg
     3721 atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag
     3781 ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac
     3841 gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg
     3901 accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag
     3961 aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg
     4021 aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa
     4081 gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag
     4141 gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca
     4201 ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac
     4261 gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg
     4321 aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag
     4381 tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt
     4441 aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt
     4501 gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg
     4561 gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc
     4621 agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc
     4681 gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc
     4741 cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg
     4801 gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat cgtgcctcag
     4861 agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg
     4921 ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg
     4981 cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag
     5041 agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc
     5101 cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac
     5161 gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc
     5221 gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc
     5281 cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg
     5341 gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag
     5401 agcgagcaga gcggcgggag cggcgggagc ggggggagca ctaatctgag cgacatcatt
     5461 gagaaggaga ctgggaaaca gctggtcatt caggagtcca tcctgatgct gcctgaggag
     5521 gtggaggaag tgatcggcaa caagccagag tctgacatcc tggtgcacac cgcctacgac
     5581 gagtccacag atgagaatgt gatgctgctg acctctgacg cccccgagta taagccttgg
     5641 gccctggtca tccaggattc taacggcgag aataagatca agatgctgag cggaggatcc
     5701 ggaggatctg gaggcagcac caacctgtct gacatcatcg agaaggagac aggcaagcag
     5761 ctggtcatcc aggagagcat cctgatgctg cccgaagaag tcgaagaagt gatcggaaac
     5821 aagcctgaga gcgatatcct ggtccatacc gcctacgacg agagtaccga cgaaaatgtg
     5881 atgctgctga catccgacgc cccagagtat aagccctggg ctctggtcat ccaggattcc
     5941 aacggagaga acaaaatcaa aatgctgtct ggcggctcaa aaagaaccgc cgacggcagc
     6001 gaattcgagc ccaagaagaa gaggaaagtc taaccggtca tcatcaccat caccattgag
     6061 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     6121 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     6181 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     6241 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     6301 gctctatggc ttctgaggcg gaaagaacca gctggggctc gataccgtcg acctctagct
     6361 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6421 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctagggtgcc taatgagtga
     6481 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6541 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6601 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6661 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6721 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6781 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6841 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6901 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6961 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7021 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7081 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7141 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7201 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     7261 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7321 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7381 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7441 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7501 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7561 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7621 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7681 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7741 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7801 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7861 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7921 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7981 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8041 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8101 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     8161 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8221 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8281 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8341 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8401 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8461 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8521 aagtgccacc tgacgtcgac ggatcgggag atcgatctcc cgatccccta gggtcgactc
     8581 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     8641 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
     8701 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
     8761 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
     8821 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     8881 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     8941 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     9001 acttggcagt acatcaagtg tatc
//
