LOCUS       pFP.R265                5683 bp ds-DNA     circular     18-DEC-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1298..1327
                     /label="P(araBAD)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             1365..2573
                     /label="mCherryN(VDA).M86.(SDL)mCherryC.H6"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1365..1841
                     /label="mCherryN"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2553..2570
                     /label="H6"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        complement(1168..1245)
                     /label="iPC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             complement(146..1075)
                     /label="araC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1344..1358
                     /label="RBS30"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3158..3982
                     /label="p15A ori"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             4411..5601
                     /label="TetR (marker)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1851..2312
                     /label="SspDnaB M86  (artificially mini)"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1842..1850
                     /label="VDA"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2322..2552
                     /label="mCherryC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2313..2321
                     /label="SDL"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 tgccacctga cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc
       61 acccgtgaag gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga
      121 ctcgaattcg cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt
      181 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg
      241 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg
      301 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc
      361 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct
      421 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag
      481 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc
      541 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt
      601 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg
      661 ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg
      721 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt
      781 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc
      841 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca
      901 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac
      961 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag
     1021 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac
     1081 attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta
     1141 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg
     1201 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg
     1261 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct
     1321 ctactgtttc tccattacta gagattaaag aggagaaata ctagatggtg agcaagggcg
     1381 aagaagacaa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     1441 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     1501 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     1561 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     1621 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     1681 acggcggcgt ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca
     1741 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     1801 tgggctggga ggcctcctcc gagcggatgt accccgagga cgtggatgct tgcatctcgg
     1861 gagatagttt gatcagcttg gcgagcacag ggaaaagagt tcctattaag gatttgttag
     1921 gcgaaaaaga ttttgaaata tgggcaatta atgaacagac gatgaagctg gaatcagcta
     1981 aagttagtcg tgtattttgt accggcaaaa agctagtcta tactctaaaa actcgactag
     2041 gtagaactat caaggcaaca gcaaatcata gatttttaac tattgatggt tggaaaagat
     2101 tagatgagct atctttaaaa gagcatattg ctctaccccg taaactagaa agctcctctt
     2161 tacaattggc accagaaata gaaaagttgc ctcagagtga tatttactgg gaccccatcg
     2221 tttctattac ggagactgga gtcgaagagg tttttgattt gactgtgcca ggactacgta
     2281 actttgtcgc caatgacatc attgtacata acagcgatct gggcgccctg aagggcgaga
     2341 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
     2401 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
     2461 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
     2521 actccaccgg cggcatggac gagctgtaca agcatcatca ccatcaccac taataatact
     2581 agtagcggcc gctgcaggag tcactaaggg ttagttagtt agattagcag aaagtcaaaa
     2641 gcctccgacc ggaggctttt gactaaaact tcccttgggg ttatcattgg ggctcactca
     2701 aaggcggtaa tcagataaaa aaaatcctta gctttcgcta aggatgattt ctgctagaga
     2761 tggaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     2821 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     2881 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     2941 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     3001 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3061 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3121 ttaacgtgag ttttcgttcc actgagcgtc agacccctta ataagatgat cttcttgaga
     3181 tcgttttggt ctgcgcgtaa tctcttgctc tgaaaacgaa aaaaccgcct tgcagggcgg
     3241 tttttcgaag gttctctgag ctaccaactc tttgaaccga ggtaactggc ttggaggagc
     3301 gcagtcacca aaacttgtcc tttcagttta gccttaaccg gcgcatgact tcaagactaa
     3361 ctcctctaaa tcaattacca gtggctgctg ccagtggtgc ttttgcatgt ctttccgggt
     3421 tggactcaag acgatagtta ccggataagg cgcagcggtc ggactgaacg gggggttcgt
     3481 gcatacagtc cagcttggag cgaactgcct acccggaact gagtgtcagg cgtggaatga
     3541 gacaaacgcg gccataacag cggaatgaca ccggtaaacc gaaaggcagg aacaggagag
     3601 cgcacgaggg agccgccagg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     3661 accactgatt tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa
     3721 acggctttgc cgcggccctc tcacttccct gttaagtatc ttcctggcat cttccaggaa
     3781 atctccgccc cgttcgtaag ccatttccgc tcgccgcagt cgaacgaccg agcgtagcga
     3841 gtcagtgagc gaggaagcgg aatatatcct gtatcacata ttctgctgac gcaccggtgc
     3901 agcctttttt ctcctgccac atgaagcact tcactgacac cctcatcagt gccaacatag
     3961 taagccagta tacactccgc tagcgctgag gtctgcctcg tgaagaaggt gttgctgact
     4021 cataccaggc ctgaatcgcc ccatcatcca gccagaaagt gagggagcca cggttgatga
     4081 gagctttgtt gtaggtggac cagttggtga ttttgaactt ttgctttgcc acggaacggt
     4141 ctgcgttgtc gggaagatgc gtgatctgat ccttcaactc agcaaaagtt cgatttattc
     4201 aacaaagcca cgttgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat
     4261 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gtttactaga
     4321 ggagattctc atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag
     4381 ttaaattgct aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatt
     4441 ctcggcaccg tcaccctgga cgctgtaggc ataggcttgg ttatgccggt actgccgggc
     4501 ctcttgcggg atatcgtcca ttccgacagt attgccagtc actatggcgt gctgcttgcg
     4561 ctctatgcgt tgatgcaatt tctttgcgca cccgttctcg gagccctgtc cgaccgcttt
     4621 ggccgccgtc cagtcctgct cgcttcgctc cttggagcca ctatcgacta cgcgatcatg
     4681 gcgaccacac ccgtcctgtg gattctctac gccggacgca tcgtggcggg catcacgggt
     4741 gccacaggtg cggttgctgg tgcctatatc gccgacatca ccgatgggga agatcgggct
     4801 cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc
     4861 gggggactgt tgggtgccat ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac
     4921 ggcctcaacc tcctcctggg ctgcttcctt atgcaggaat cgcataaggg agagcgccgt
     4981 ccgatgccct tgcgtgcctt caatccagtc agctccttcc ggtgggcgcg gggcatgact
     5041 atcgtcgccg cacttatgac tgttttcttt atcatgcaac tcgtaggaca ggttccggca
     5101 gcgctctggg tcattttcgg cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg
     5161 tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag ccttcgtcac gggccccgcc
     5221 accaaacgtt tcggcgagaa gcaggccatt atcgcgggca tggcggccga cgcgctgggc
     5281 tacgtcttgc tggcgttcgc gacgcgcggc tggatggcct tccccattat gattcttctc
     5341 gcttccggcg gcatcggtat gcccgcgttg caggccatgc tgtcccgcca agtagatgac
     5401 gaccatcagg gacagcttca agggtcgctc gcggctctta ccagcctcac ttcgatcatt
     5461 ggaccgctga tcgtcacggc gatttatgcc gcctcggcga gcacatggaa cgggttggca
     5521 tggattgtag gtgccgccct ttaccttgtc tgcctccccg cgttgcgtcg cggtgcatgg
     5581 agccgggcca cctcgaccta ataatactag ctccggcaaa aaaacgggca aggtgtcacc
     5641 accctgccct ttttctttaa aaccgaaaag attacttcgc gtt
//