LOCUS       pcDNA3-Cerulean-FHA1-NES    6660 bp      DNA     circular   3/1/19
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=Degen. DNA
COMMENT     SerialCloner_Comments=Ligation of : pcDNA3-AKAR4-NES.gb.xdna [5497  nt] : (EcoRI[2
            982] / BamHI[1002]) to Untitled Sequence #1 [721  nt] : (Bam
            HI[115] / SphI[840]) and to Sequence Window #3 [430  nt] : (
            SphI[708] / EcoRI[1142])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    2167..2193
                     /label=NES-Targeting
                     /SerialCloner_Color=&h800040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             922..939
                     /label=6His
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             1735..2157
                     /label=FHA1
                     /SerialCloner_Color=&h804000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             1009..1725
                     /label=CER
                     /SerialCloner_Color=&h66CCFE
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(4499..4519)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(2213..2230)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(4887..5515)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(4525..4547)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(5667..6326)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             3368..4159
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(6566..6594)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(4552..4581)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    3149..3226
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      2253..2480
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     promoter        232..819
                     /label=CMV prom
                     /SerialCloner_Color=&h800080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 
      901 GCCACCATGG TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT 
      961 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGgatcCCAT GGTGAGCAAG 
     1021 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC 
     1081 GGCCACAGGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC 
     1141 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC 
     1201 CTGACCTGGG GCGTGCAGTG CTTCGCCCGC TACCCCGACC ACATGAAGCA GCACGACTTC 
     1261 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGTA CCATCTTCTT CAAGGACGAC 
     1321 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC 
     1381 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCATAA GCTGGAGTAC 
     1441 AACGCCATCA GTGACAACGT CTATATCACC GCCGACAAGC AGAAGAACGG CATCAAGGCC 
     1501 CACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG 
     1561 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC 
     1621 CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC 
     1681 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGCGcat gCATAAGTTT 
     1741 TCTCAAGAAC AGATCGGCGA AAACATTGTG TGCAGGGTCA TTTGTACCAC GGGTCAAATT 
     1801 CCCATCCGAG ATTTGTCAGC TGATATTTCA CAAGTGCTTA AGGAAAAACG ATCCATAAAG 
     1861 AAAGTTTGGA CATTTGGTAG AAACCCAGCC TGTGACTATC ATTTAGGAAA CATTTCAAGA 
     1921 CTGTCAAATA AGCATTTCCA AATACTACTA GGAGAAGACG GTAACCTTTT ATTGAATGAC 
     1981 ATTTCCACTA ATGGGACCTG GTTAAATGGG CAAAAAGTCG AGAAGAACAG CAATCAGTTA 
     2041 CTGTCTCAAG GTGATGAAAT AACCGTTGGT GTAGGCGTGG AATCAGATAT TTTATCTCTG 
     2101 GTCATTTTCA TAAACGACAA ATTTAAGCAG TGCCTCGAGC AGAACAAAGT TGATCGCTCT 
     2161 GaattCCTGC CCCCCCTGGA GCGCCTGACC CTGTAATCTA GAGGGCCCTA TTctatagtg 
     2221 tcacctaaat gctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc 
     2281 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct 
     2341 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg 
     2401 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg 
     2461 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta 
     2521 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 
     2581 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 
     2641 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg ggcatccctt tagggttccg 
     2701 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 
     2761 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 
     2821 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga 
     2881 tttataaggg attttgggga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 
     2941 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc 
     3001 tccccaggca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg 
     3061 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 
     3121 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 
     3181 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc 
     3241 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa 
     3301 gctcccggga gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt 
     3361 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct 
     3421 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct 
     3481 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga 
     3541 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc 
     3601 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg 
     3661 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc 
     3721 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca 
     3781 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga 
     3841 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc 
     3901 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga 
     3961 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca 
     4021 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg 
     4081 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct 
     4141 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc 
     4201 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga 
     4261 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc 
     4321 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 
     4381 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 
     4441 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca 
     4501 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 
     4561 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt 
     4621 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 
     4681 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 
     4741 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 
     4801 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 
     4861 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 
     4921 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 
     4981 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 
     5041 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa 
     5101 tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 
     5161 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 
     5221 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 
     5281 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 
     5341 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 
     5401 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 
     5461 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 
     5521 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 
     5581 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 
     5641 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 
     5701 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 
     5761 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 
     5821 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 
     5881 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 
     5941 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc 
     6001 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 
     6061 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 
     6121 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 
     6181 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 
     6241 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca 
     6301 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 
     6361 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 
     6421 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 
     6481 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 
     6541 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 
     6601 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 
     6661 
//