LOCUS       pcDNA3-PKCsub-YPet-NES    6294 bp      DNA     circular   3/1/19
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=Degen. DNA
COMMENT     SerialCloner_Comments=Ligation of : pcDNA3-Cerulean-FHA1-NES [5461  nt] : (XbaI[21
            97] / BamHI[1002]) to Untitled Sequence #1 [825  nt] : (BamH
            I[114] / XbaI[943])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     CDS             922..939
                     /label=6His
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(4133..4153)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(4521..5149)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(4159..4181)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(5301..5960)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             3002..3793
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(6200..6228)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(4186..4215)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    2783..2860
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      1887..2114
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     promoter        232..819
                     /label=CMV prom
                     /SerialCloner_Color=&h800080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1801..1827
                     /label=NES-Targeting
                     /SerialCloner_Color=&h800040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    1012..1053
                     /label=PKCsub
                     /SerialCloner_Color=&h999999
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     gene            1078..1794
                     /label=YPET
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(1847..1864)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 
      901 GCCACCATGG TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT 
      961 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGgatcCCAT GCGATTCAGA 
     1021 AGATTCCAGA CGTTGAAGGA CAAGGCTAAG GCTGGCGGCA CCGGCGGCAG CGAGCTCATG 
     1081 GTGAGCAAAG GCGAAGAGCT GTTCACCGGC GTGGTGCCCA TCCTGGTGGA GCTGGACGGC 
     1141 GACGTGAACG GCCACAAGTT CAGCGTGAGC GGCGAGGGCG AGGGCGACGC CACCTACGGC 
     1201 AAGCTGACCC TGAAGCTGCT GTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTG 
     1261 GTGACCACCC TGGGCTACGG CGTGCAGTGC TTCGCCCGGT ACCCCGACCA CATGAAGCAG 
     1321 CACGACTTCT TCAAGAGCGC CATGCCCGAG GGCTACGTGC AGGAGCGGAC CATCTTCTTC 
     1381 AAGGACGACG GCAACTACAA GACCCGGGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG 
     1441 AACCGGATCG AGCTGAAGGG CATCGACTTC AAGGAGGACA GCAACATCCT GGGCCACAAG 
     1501 CTGGAGTACA ACTACAACAG CCACAACGTG TACATCACCG CCGACAAGCA GAAGAACGGC 
     1561 ATCAAGGCCA ACTTCAAGAT CCGGCACAAC ATCGAGGACG GCGGCGTGCA GCTGGCCGAC 
     1621 CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA TAACCACTAC 
     1681 CTGAGCTACC AGAGCGCCCT GTTCAAGGAC CCCAACGAGA AGCGGGACCA CATGGTGCTG 
     1741 CTGGAGTTCC TGACCGCCGC CGGCATCACC GAGGGTATGA ATGAATTGTA CAAAGAATTC 
     1801 CTGCCCCCCC TGGAGCGCCT GACCCTGTAA TctagAGGGC CCTATTctat agtgtcacct 
     1861 aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt 
     1921 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 
     1981 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 
     2041 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 
     2101 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca 
     2161 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 
     2221 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 
     2281 gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag 
     2341 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 
     2401 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 
     2461 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 
     2521 agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 
     2581 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 
     2641 ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 
     2701 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 
     2761 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 
     2821 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 
     2881 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 
     2941 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat 
     3001 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 
     3061 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 
     3121 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 
     3181 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 
     3241 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 
     3301 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 
     3361 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 
     3421 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 
     3481 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 
     3541 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 
     3601 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 
     3661 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 
     3721 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 
     3781 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg 
     3841 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 
     3901 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 
     3961 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 
     4021 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 
     4081 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca 
     4141 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 
     4201 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 
     4261 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 
     4321 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 
     4381 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 
     4441 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 
     4501 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 
     4561 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 
     4621 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 
     4681 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca 
     4741 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 
     4801 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 
     4861 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 
     4921 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 
     4981 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 
     5041 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 
     5101 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 
     5161 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 
     5221 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 
     5281 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 
     5341 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 
     5401 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 
     5461 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 
     5521 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 
     5581 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 
     5641 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 
     5701 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 
     5761 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 
     5821 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 
     5881 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 
     5941 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 
     6001 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 
     6061 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 
     6121 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 
     6181 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 
     6241 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
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