LOCUS       pcDNA3-sACt-mCherry-NLS    7698 bp      DNA     circular   12/17/19
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     misc_feature    3157..3186
                     /label=NLS-Targeting
                     /SerialCloner_Color=&h000080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             922..939
                     /label=6His
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             1036..2442
                     /label=rat sACt
                     /SerialCloner_Color=&hFC6665
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             2449..3156
                     /label=mCherry
                     /SerialCloner_Color=&hFF0000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(5537..5557)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     primer_bind     complement(3251..3268)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     rep_origin      complement(5925..6553)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_binding    complement(5563..5585)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             complement(6705..7364)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     CDS             4406..5197
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(7604..7632)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     Promoter        complement(5590..5619)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     misc_feature    4187..4264
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     terminator      3291..3518
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
     promoter        232..819
                     /label=CMV prom
                     /SerialCloner_Color=&h800080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 
      901 GCCACCATGG TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT 
      961 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGGATCCGAG CTCGAGATCT 
     1021 GCAGCTGCCA TGGACatgag tgcccgaagg caggaattac aggacagggc aatcgtcaag 
     1081 atagctgctc acttaccgga cctcattgtc tatggagatt tctctcccga gcggccgtca 
     1141 gtgaaatgtt ttgatggagt tctgatgttt gtcgatattt caggctttac tgcaatgact 
     1201 gagaagttca gcacagccat gtacatggac cgaggagccg agcagctggt ggagatcctc 
     1261 aactactaca taagtgcgat agtggagaaa gtactgattt ttggaggaga catcctaaaa 
     1321 tttgcaggtg acgccttgtt ggccctgtgg aaagtggaac gaaagcaact gaagaatatc 
     1381 atcacggtgg taattaaatg cagcctggag attcatggct tgtttgaagc caaggaggtt 
     1441 gaagaaggcc tggatattcg agttaagata ggactggctg ctggccacat caccatgttg 
     1501 gtctttgggg atgaaacacg gaactacttc ctggtgattg gccaagcggt ggatgatgta 
     1561 cgccttgctc agaacatggc tcagatgaat gatgttattc tgtcaccaaa ctgctggcag 
     1621 ctctgtgatc ggagcatgat tgaaatcgag aggattccgg atcagagagc agttaaggtt 
     1681 agcttcttaa aaccaccccc aacttttaac ttcgacgagt tttttgccaa gtgtatggcc 
     1741 ttcatggatt attatccttc tggtgaccac aaaaacttcc taaggcttgc ctgcatgctg 
     1801 gagtctgatc ctgaactcga gttgtctcta caaaagtatg tgatggaaat cattttgaag 
     1861 cagattgatg acaagcagct tcggggctat ttatctgagc ttcgtcctgt gacgatcgtg 
     1921 tttgtgaact tgatgtttaa agagcaagac aaagcagaag tcataggatc agccatccaa 
     1981 gctgcctgtg tgcacatcac ttccgtcttg aaggtcttcc gaggccagat caataaggtc 
     2041 ttcatgtttg ataagggctg ctccttcctc tgtgtcttcg gtttccctgg ggaaaaggcc 
     2101 cctgacgaga tcactcacgc tttggaaagt gccgtggata tattcgactt ctgctctcag 
     2161 gtccacaaaa tccgtactgt ctccatcggc gtcgccagtg ggattgtctt ctgtgggatc 
     2221 gttggacaca ctgtgagaca cgagtacaca gtcattggcc aaaaggtcaa tattgctgcc 
     2281 aggatgatga tgtattaccc aggcatcgtg acctgcgact ctgtcacata cgatggcagc 
     2341 aacctgccag cctacttttt taaagagctt ccaaagaaag tcatgaaagg agttgcggac 
     2401 cccggaccag tgtatcagtg tctgggcctc aatgagaaag tcGTCGACAT GGTGAGCAAG 
     2461 GGCGAAGAGG ATAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAG 
     2521 GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG 
     2581 GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC CCCTGCCCTT CGCCTGGGAC 
     2641 ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG TGAAGCACCC CGCCGACATC 
     2701 CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC 
     2761 GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG CGAGTTCATC 
     2821 TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG 
     2881 ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC 
     2941 GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCTGA GGTCAAGACC 
     3001 ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG 
     3061 GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC 
     3121 CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGCCAA AAAAGAAGAG AAAGGTGGAA 
     3181 GACGCATAAG AATTCTGCAG ATATCCATCA CACTGGCGGC CGCTCGAGCA TGCATCTAGA 
     3241 GGGCCCTATT ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc 
     3301 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg 
     3361 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag 
     3421 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga 
     3481 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag 
     3541 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 
     3601 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 
     3661 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 
     3721 catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 
     3781 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 
     3841 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 
     3901 ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa 
     3961 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg 
     4021 tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc atctcaatta 
     4081 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 
     4141 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac 
     4201 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 
     4261 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 
     4321 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga 
     4381 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc 
     4441 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 
     4501 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 
     4561 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac 
     4621 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 
     4681 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 
     4741 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 
     4801 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 
     4861 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 
     4921 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 
     4981 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 
     5041 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 
     5101 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 
     5161 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg 
     5221 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat 
     5281 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 
     5341 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac 
     5401 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 
     5461 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc 
     5521 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 
     5581 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 
     5641 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 
     5701 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 
     5761 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 
     5821 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 
     5881 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 
     5941 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 
     6001 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 
     6061 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 
     6121 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 
     6181 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 
     6241 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 
     6301 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 
     6361 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 
     6421 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 
     6481 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 
     6541 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 
     6601 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 
     6661 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 
     6721 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 
     6781 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 
     6841 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 
     6901 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 
     6961 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 
     7021 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 
     7081 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 
     7141 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 
     7201 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 
     7261 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 
     7321 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 
     7381 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 
     7441 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 
     7501 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 
     7561 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 
     7621 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 
     7681 aaagtgccac ctgacgtc
//