LOCUS       pFP.R272                5692 bp ds-DNA     circular     18-DEC-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    2562..2579
                     /label="H6"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(146..1075)
                     /label="araC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             4420..5610
                     /label="TetR (marker)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2331..2561
                     /label="mCherryC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        complement(1168..1245)
                     /label="iPC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1344..1358
                     /label="RBS30"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             1365..2582
                     /label="mCherryN(AGG).SspGyrB.(SAK)mCherryC.H6"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1365..1841
                     /label="mCherryN"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2322..2330
                     /label="SAK"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    1851..2321
                     /label="SspGyrB (artificially mini)"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3167..3991
                     /label="p15A ori"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1842..1850
                     /label="AGG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1298..1327
                     /label="P(araBAD)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 tgccacctga cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc
       61 acccgtgaag gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga
      121 ctcgaattcg cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt
      181 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg
      241 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg
      301 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc
      361 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct
      421 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag
      481 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc
      541 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt
      601 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg
      661 ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg
      721 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt
      781 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc
      841 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca
      901 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac
      961 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag
     1021 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac
     1081 attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta
     1141 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg
     1201 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg
     1261 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct
     1321 ctactgtttc tccattacta gagattaaag aggagaaata ctagatggtg agcaagggcg
     1381 aagaagacaa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     1441 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     1501 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     1561 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     1621 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     1681 acggcggcgt ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca
     1741 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     1801 tgggctggga ggcctcctcc gagcggatgt accccgagga cgcaggtggt tgtttttctg
     1861 gagatacatt agtcgcttta actgatggtc gtagcgttag ctttgagcaa ttggttgaag
     1921 aagaaaaaca aggaaaacaa aacttttgtt ataccatccg ccatgatggt tctatagggg
     1981 ttgaaaaaat catcaatgcc cgcaaaacaa aaactaatgc gaaggtaatc aaggttacgt
     2041 tggacaatgg tgagtctatt atttgcaccc cggatcataa attcatgttg cgggatggga
     2101 gctacaaatg tgcgatggat ttaactctcg atgattcgtt aatgccgtta caccgaaaaa
     2161 tttcgactac ggaagattct ggtcatgcga tggaagcagt attaaattac aatcacagaa
     2221 ttgtaaatat tgaagctgtg tcagaaacaa tcgatgttta tgatattgag gttccccaca
     2281 cccacaattt tgctttggca agcggagtgt ttgtccataa cagtgcaaaa ggcgccctga
     2341 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
     2401 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
     2461 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     2521 agggccgcca ctccaccggc ggcatggacg agctgtacaa gcatcatcac catcaccact
     2581 aataatacta gtagcggccg ctgcaggagt cactaagggt tagttagtta gattagcaga
     2641 aagtcaaaag cctccgaccg gaggcttttg actaaaactt cccttggggt tatcattggg
     2701 gctcactcaa aggcggtaat cagataaaaa aaatccttag ctttcgctaa ggatgatttc
     2761 tgctagagat ggaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     2821 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     2881 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     2941 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     3001 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     3061 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     3121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccttaa taagatgatc
     3181 ttcttgagat cgttttggtc tgcgcgtaat ctcttgctct gaaaacgaaa aaaccgcctt
     3241 gcagggcggt ttttcgaagg ttctctgagc taccaactct ttgaaccgag gtaactggct
     3301 tggaggagcg cagtcaccaa aacttgtcct ttcagtttag ccttaaccgg cgcatgactt
     3361 caagactaac tcctctaaat caattaccag tggctgctgc cagtggtgct tttgcatgtc
     3421 tttccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg gactgaacgg
     3481 ggggttcgtg catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc
     3541 gtggaatgag acaaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga
     3601 acaggagagc gcacgaggga gccgccaggg gaaacgcctg gtatctttat agtcctgtcg
     3661 ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc
     3721 tatggaaaaa cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc
     3781 ttccaggaaa tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga
     3841 gcgtagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg
     3901 caccggtgca gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg
     3961 ccaacatagt aagccagtat acactccgct agcgctgagg tctgcctcgt gaagaaggtg
     4021 ttgctgactc ataccaggcc tgaatcgccc catcatccag ccagaaagtg agggagccac
     4081 ggttgatgag agctttgttg taggtggacc agttggtgat tttgaacttt tgctttgcca
     4141 cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca gcaaaagttc
     4201 gatttattca acaaagccac gttgtgtctc aaaatctctg atgttacatt gcacaagata
     4261 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
     4321 tttactagag gagattctca tgtttgacag cttatcatcg ataagcttta atgcggtagt
     4381 ttatcacagt taaattgcta acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc
     4441 atcgtcattc tcggcaccgt caccctggac gctgtaggca taggcttggt tatgccggta
     4501 ctgccgggcc tcttgcggga tatcgtccat tccgacagta ttgccagtca ctatggcgtg
     4561 ctgcttgcgc tctatgcgtt gatgcaattt ctttgcgcac ccgttctcgg agccctgtcc
     4621 gaccgctttg gccgccgtcc agtcctgctc gcttcgctcc ttggagccac tatcgactac
     4681 gcgatcatgg cgaccacacc cgtcctgtgg attctctacg ccggacgcat cgtggcgggc
     4741 atcacgggtg ccacaggtgc ggttgctggt gcctatatcg ccgacatcac cgatggggaa
     4801 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
     4861 cccgtggccg ggggactgtt gggtgccatc tccttgcatg caccattcct tgcggcggcg
     4921 gtgctcaacg gcctcaacct cctcctgggc tgcttcctta tgcaggaatc gcataaggga
     4981 gagcgccgtc cgatgccctt gcgtgccttc aatccagtca gctccttccg gtgggcgcgg
     5041 ggcatgacta tcgtcgccgc acttatgact gttttcttta tcatgcaact cgtaggacag
     5101 gttccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg
     5161 atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcacg
     5221 ggccccgcca ccaaacgttt cggcgagaag caggccatta tcgcgggcat ggcggccgac
     5281 gcgctgggct acgtcttgct ggcgttcgcg acgcgcggct ggatggcctt ccccattatg
     5341 attcttctcg cttccggcgg catcggtatg cccgcgttgc aggccatgct gtcccgccaa
     5401 gtagatgacg accatcaggg acagcttcaa gggtcgctcg cggctcttac cagcctcact
     5461 tcgatcattg gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac
     5521 gggttggcat ggattgtagg tgccgccctt taccttgtct gcctccccgc gttgcgtcgc
     5581 ggtgcatgga gccgggccac ctcgacctaa taatactagc tccggcaaaa aaacgggcaa
     5641 ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg tt
//