LOCUS       pFP.R361                5695 bp ds-DNA     circular     19-DEC-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     CDS             1365..2585
                     /label="mCherryN(SNL).BsuP-RIR1*.(CSE)mCherryC.H6"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1298..1327
                     /label="P(araBAD)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2325..2333
                     /label="CSE"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1344..1358
                     /label="RBS30"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3170..3994
                     /label="p15A ori"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             complement(146..1075)
                     /label="araC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1851..2324
                     /label="BsuP-M1918_RIR1 (artificially mini)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2565..2582
                     /label="H6"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2334..2564
                     /label="mCherryC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        complement(1168..1245)
                     /label="iPC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             4423..5613
                     /label="TetR (marker)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1842..1850
                     /label="SNL"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2184..2213
                     /label="Linker"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1365..1841
                     /label="mCherryN"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
ORIGIN
        1 tgccacctga cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc
       61 acccgtgaag gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga
      121 ctcgaattcg cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt
      181 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg
      241 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg
      301 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc
      361 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct
      421 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag
      481 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc
      541 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt
      601 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg
      661 ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg
      721 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt
      781 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc
      841 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca
      901 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac
      961 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag
     1021 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac
     1081 attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta
     1141 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg
     1201 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg
     1261 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct
     1321 ctactgtttc tccattacta gagattaaag aggagaaata ctagatggtg agcaagggcg
     1381 aagaagacaa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     1441 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     1501 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     1561 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     1621 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     1681 acggcggcgt ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca
     1741 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     1801 tgggctggga ggcctcctcc gagcggatgt accccgagga cagcaatctg tgtgtgacgg
     1861 gtgaaaccct ccttctgacc gagaacggct atgaaaaagc tgctgatctc tataagaagc
     1921 agaataattt aaaagttgtc atcgataata ggacaaagga cttcgcggta gatagtaaag
     1981 ggaccactat tgtagatgct attccaatgc aattgacgaa aaaagatgcc gaaattttta
     2041 aagttaaaac taagcagggg tatgaaattc gggctacgga atggcataag ttttatgtta
     2101 aacgcgacgg tgagattcag aaattacaac tgaatcagct gaagacaggt gataagctcc
     2161 tggtccagag tgccgagggc gctggcagcg caggttccgc cgctggctca ggtgatttta
     2221 cggcggagat tatctctatt gaagaagatg gagtggaaga cgtatatgat acaactcaag
     2281 aggattatca ttcgctgatt tttaacggca ttgtaactgg taattgcagt gaaggcgccc
     2341 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     2401 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     2461 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     2521 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagcatcat caccatcacc
     2581 actaataata ctagtagcgg ccgctgcagg agtcactaag ggttagttag ttagattagc
     2641 agaaagtcaa aagcctccga ccggaggctt ttgactaaaa cttcccttgg ggttatcatt
     2701 ggggctcact caaaggcggt aatcagataa aaaaaatcct tagctttcgc taaggatgat
     2761 ttctgctaga gatggaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     2821 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     2881 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     2941 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     3001 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     3061 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     3121 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagacccct taataagatg
     3181 atcttcttga gatcgttttg gtctgcgcgt aatctcttgc tctgaaaacg aaaaaaccgc
     3241 cttgcagggc ggtttttcga aggttctctg agctaccaac tctttgaacc gaggtaactg
     3301 gcttggagga gcgcagtcac caaaacttgt cctttcagtt tagccttaac cggcgcatga
     3361 cttcaagact aactcctcta aatcaattac cagtggctgc tgccagtggt gcttttgcat
     3421 gtctttccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcggactgaa
     3481 cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca
     3541 ggcgtggaat gagacaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca
     3601 ggaacaggag agcgcacgag ggagccgcca ggggaaacgc ctggtatctt tatagtcctg
     3661 tcgggtttcg ccaccactga tttgagcgtc agatttcgtg atgcttgtca ggggggcgga
     3721 gcctatggaa aaacggcttt gccgcggccc tctcacttcc ctgttaagta tcttcctggc
     3781 atcttccagg aaatctccgc cccgttcgta agccatttcc gctcgccgca gtcgaacgac
     3841 cgagcgtagc gagtcagtga gcgaggaagc ggaatatatc ctgtatcaca tattctgctg
     3901 acgcaccggt gcagcctttt ttctcctgcc acatgaagca cttcactgac accctcatca
     3961 gtgccaacat agtaagccag tatacactcc gctagcgctg aggtctgcct cgtgaagaag
     4021 gtgttgctga ctcataccag gcctgaatcg ccccatcatc cagccagaaa gtgagggagc
     4081 cacggttgat gagagctttg ttgtaggtgg accagttggt gattttgaac ttttgctttg
     4141 ccacggaacg gtctgcgttg tcgggaagat gcgtgatctg atccttcaac tcagcaaaag
     4201 ttcgatttat tcaacaaagc cacgttgtgt ctcaaaatct ctgatgttac attgcacaag
     4261 ataaaaatat atcatcatga acaataaaac tgtctgctta cataaacagt aatacaaggg
     4321 gtgtttacta gaggagattc tcatgtttga cagcttatca tcgataagct ttaatgcggt
     4381 agtttatcac agttaaattg ctaacgcagt caggcaccgt gtatgaaatc taacaatgcg
     4441 ctcatcgtca ttctcggcac cgtcaccctg gacgctgtag gcataggctt ggttatgccg
     4501 gtactgccgg gcctcttgcg ggatatcgtc cattccgaca gtattgccag tcactatggc
     4561 gtgctgcttg cgctctatgc gttgatgcaa tttctttgcg cacccgttct cggagccctg
     4621 tccgaccgct ttggccgccg tccagtcctg ctcgcttcgc tccttggagc cactatcgac
     4681 tacgcgatca tggcgaccac acccgtcctg tggattctct acgccggacg catcgtggcg
     4741 ggcatcacgg gtgccacagg tgcggttgct ggtgcctata tcgccgacat caccgatggg
     4801 gaagatcggg ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca
     4861 ggccccgtgg ccgggggact gttgggtgcc atctccttgc atgcaccatt ccttgcggcg
     4921 gcggtgctca acggcctcaa cctcctcctg ggctgcttcc ttatgcagga atcgcataag
     4981 ggagagcgcc gtccgatgcc cttgcgtgcc ttcaatccag tcagctcctt ccggtgggcg
     5041 cggggcatga ctatcgtcgc cgcacttatg actgttttct ttatcatgca actcgtagga
     5101 caggttccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg
     5161 atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc
     5221 acgggccccg ccaccaaacg tttcggcgag aagcaggcca ttatcgcggg catggcggcc
     5281 gacgcgctgg gctacgtctt gctggcgttc gcgacgcgcg gctggatggc cttccccatt
     5341 atgattcttc tcgcttccgg cggcatcggt atgcccgcgt tgcaggccat gctgtcccgc
     5401 caagtagatg acgaccatca gggacagctt caagggtcgc tcgcggctct taccagcctc
     5461 acttcgatca ttggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg
     5521 aacgggttgg catggattgt aggtgccgcc ctttaccttg tctgcctccc cgcgttgcgt
     5581 cgcggtgcat ggagccgggc cacctcgacc taataatact agctccggca aaaaaacggg
     5641 caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgtt
//