LOCUS       pFP.R363                5770 bp ds-DNA     circular     19-DEC-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    3245..4069
                     /label="p15A ori"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2640..2657
                     /label="H6"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             complement(146..1075)
                     /label="araC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1851..2399
                     /label="Ckl_PTerm (artificially mini)"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        complement(1168..1245)
                     /label="iPC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1298..1327
                     /label="P(araBAD)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1842..1850
                     /label="NGK"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1365..1841
                     /label="mCherryN"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             4498..5688
                     /label="TetR (marker)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             1365..2660
                     /label="mCherryN(NGK).Ckl-Ter*.(TTL)mCherryC.H6"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1344..1358
                     /label="RBS30"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2400..2408
                     /label="TTL"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2409..2639
                     /label="mCherryC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2253..2282
                     /label="Linker"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 tgccacctga cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc
       61 acccgtgaag gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga
      121 ctcgaattcg cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt
      181 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg
      241 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg
      301 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc
      361 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct
      421 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag
      481 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc
      541 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt
      601 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg
      661 ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg
      721 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt
      781 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc
      841 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca
      901 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac
      961 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag
     1021 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac
     1081 attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta
     1141 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg
     1201 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg
     1261 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct
     1321 ctactgtttc tccattacta gagattaaag aggagaaata ctagatggtg agcaagggcg
     1381 aagaagacaa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     1441 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     1501 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     1561 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     1621 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     1681 acggcggcgt ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca
     1741 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     1801 tgggctggga ggcctcctcc gagcggatgt accccgagga caatgggaaa gcgcttgatc
     1861 ttgacacgcc catagacacg cccacggggt ggaaaaccat gcgtgacatt gagtgtggag
     1921 actatgtttt tggagttgat ggtaaaccaa cgaaagtcat tggaacgtcg gatataatgg
     1981 ttaatcatga atgttacaaa gttacctttg aagatggaga gtttatcatc gcagataccg
     2041 agcatatttg gactgtgaca acaaaatcgt cacggaaaac cttgaaatat aagcctctta
     2101 aagggcggca acttctgcgt ccagattatc gtgaatcaaa tggatatttt gatgtaacga
     2161 ccggtgaaat ggccaagaat ttcaagcatt ttcgcaaaga cggaaagggt atcgagtata
     2221 aatacagagt accgatggcc ggcgcagttg agggtagcgc aggttcagca gcaggatcag
     2281 gtaaaaacaa atcagtaatt aacatagaaa aatacaaatc cgttcctgta aagtgtatcg
     2341 ctgtggaaga cgaaaaaaaa ttgtacctgg caggcaaaaa ccacactgcc actcacaaca
     2401 ctaccctcgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     2461 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     2521 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     2581 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagc
     2641 atcatcacca tcaccactaa taatactagt agcggccgct gcaggagtca ctaagggtta
     2701 gttagttaga ttagcagaaa gtcaaaagcc tccgaccgga ggcttttgac taaaacttcc
     2761 cttggggtta tcattggggc tcactcaaag gcggtaatca gataaaaaaa atccttagct
     2821 ttcgctaagg atgatttctg ctagagatgg aatagactgg atggaggcgg ataaagttgc
     2881 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     2941 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     3001 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     3061 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     3121 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     3181 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     3241 ccccttaata agatgatctt cttgagatcg ttttggtctg cgcgtaatct cttgctctga
     3301 aaacgaaaaa accgccttgc agggcggttt ttcgaaggtt ctctgagcta ccaactcttt
     3361 gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa cttgtccttt cagtttagcc
     3421 ttaaccggcg catgacttca agactaactc ctctaaatca attaccagtg gctgctgcca
     3481 gtggtgcttt tgcatgtctt tccgggttgg actcaagacg atagttaccg gataaggcgc
     3541 agcggtcgga ctgaacgggg ggttcgtgca tacagtccag cttggagcga actgcctacc
     3601 cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc ataacagcgg aatgacaccg
     3661 gtaaaccgaa aggcaggaac aggagagcgc acgagggagc cgccagggga aacgcctggt
     3721 atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct
     3781 tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca cttccctgtt
     3841 aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg
     3901 ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatcctgta
     3961 tcacatattc tgctgacgca ccggtgcagc cttttttctc ctgccacatg aagcacttca
     4021 ctgacaccct catcagtgcc aacatagtaa gccagtatac actccgctag cgctgaggtc
     4081 tgcctcgtga agaaggtgtt gctgactcat accaggcctg aatcgcccca tcatccagcc
     4141 agaaagtgag ggagccacgg ttgatgagag ctttgttgta ggtggaccag ttggtgattt
     4201 tgaacttttg ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct
     4261 tcaactcagc aaaagttcga tttattcaac aaagccacgt tgtgtctcaa aatctctgat
     4321 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
     4381 acagtaatac aaggggtgtt tactagagga gattctcatg tttgacagct tatcatcgat
     4441 aagctttaat gcggtagttt atcacagtta aattgctaac gcagtcaggc accgtgtatg
     4501 aaatctaaca atgcgctcat cgtcattctc ggcaccgtca ccctggacgc tgtaggcata
     4561 ggcttggtta tgccggtact gccgggcctc ttgcgggata tcgtccattc cgacagtatt
     4621 gccagtcact atggcgtgct gcttgcgctc tatgcgttga tgcaatttct ttgcgcaccc
     4681 gttctcggag ccctgtccga ccgctttggc cgccgtccag tcctgctcgc ttcgctcctt
     4741 ggagccacta tcgactacgc gatcatggcg accacacccg tcctgtggat tctctacgcc
     4801 ggacgcatcg tggcgggcat cacgggtgcc acaggtgcgg ttgctggtgc ctatatcgcc
     4861 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     4921 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gtgccatctc cttgcatgca
     4981 ccattccttg cggcggcggt gctcaacggc ctcaacctcc tcctgggctg cttccttatg
     5041 caggaatcgc ataagggaga gcgccgtccg atgcccttgc gtgccttcaa tccagtcagc
     5101 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt tttctttatc
     5161 atgcaactcg taggacaggt tccggcagcg ctctgggtca ttttcggcga ggaccgcttt
     5221 cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc
     5281 gctcaagcct tcgtcacggg ccccgccacc aaacgtttcg gcgagaagca ggccattatc
     5341 gcgggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgcggctgg
     5401 atggccttcc ccattatgat tcttctcgct tccggcggca tcggtatgcc cgcgttgcag
     5461 gccatgctgt cccgccaagt agatgacgac catcagggac agcttcaagg gtcgctcgcg
     5521 gctcttacca gcctcacttc gatcattgga ccgctgatcg tcacggcgat ttatgccgcc
     5581 tcggcgagca catggaacgg gttggcatgg attgtaggtg ccgcccttta ccttgtctgc
     5641 ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctaata atactagctc
     5701 cggcaaaaaa acgggcaagg tgtcaccacc ctgccctttt tctttaaaac cgaaaagatt
     5761 acttcgcgtt
//