LOCUS       pFP.R367                5689 bp ds-DNA     circular     20-DEC-2019
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1298..1327
                     /label="P(araBAD)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1365..1841
                     /label="mCherryN"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    2175..2204
                     /label="Linker"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3164..3988
                     /label="p15A ori"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2319..2327
                     /label="CGK"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     promoter        complement(1168..1245)
                     /label="iPC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             4417..5607
                     /label="TetR (marker)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             complement(146..1075)
                     /label="araC"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2559..2576
                     /label="H6"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             1365..2579
                     /label="mCherryN(ATG).LP-Hel*.(CGK)mCherryC.H6"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1851..2318
                     /label="LP-phiHSIC_Helicase"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    1344..1358
                     /label="RBS30"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2328..2558
                     /label="mCherryC"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1842..1850
                     /label="ATG"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
ORIGIN
        1 tgccacctga cgtctaagaa aaggaatatt cagcaatttg cccgtgccga agaaaggccc
       61 acccgtgaag gtgagccagt gagttgattg ctacgtaatt agttagttag cccttagtga
      121 ctcgaattcg cggccgcttc tagagttatg acaacttgac ggctacatca ttcacttttt
      181 cttcacaacc ggcacggaac tcgctcgggc tggccccggt gcatttttta aatacccgcg
      241 agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata ggcatccggg
      301 tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag cttaagacgc
      361 taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggcgacaag caaacatgct
      421 gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg tactgacaag
      481 cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct tccatgcgcc
      541 gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc ccttcccctt
      601 gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc gcttcatccg
      661 ggcgaaagaa ccccgtattg gcaaatattg acggccagtt aagccattca tgccagtagg
      721 cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga tgacgaccgt
      781 agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa acaaattctc
      841 gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata taacctttca
      901 ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc ggcgttaaac
      961 ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt tgcgcttcag
     1021 ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat tgcatcagac
     1081 attgccgtca ctgcgtcttt tactggctct tctcgctaac caaaccggta accccgctta
     1141 ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt aacaaaagtg
     1201 tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca ctttgctatg
     1261 ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta tcgcaactct
     1321 ctactgtttc tccattacta gagattaaag aggagaaata ctagatggtg agcaagggcg
     1381 aagaagacaa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     1441 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     1501 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     1561 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     1621 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     1681 acggcggcgt ggtgaccgtg acccaggact cctccttgca ggacggcgag ttcatctaca
     1741 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     1801 tgggctggga ggcctcctcc gagcggatgt accccgagga cgccactggg tgccatgctt
     1861 acggacatga tataatgatg tccgatggta ctaagaaaca ggttcaggat attgcagtag
     1921 gcgacaaggt tatgggtcct gatggtaatc ctagaaaagt cattcggctg gtgaaaggtc
     1981 aagatgagat gtttcgtgtt actccgacga aaggcgaatc ctttgttgta aacggtggtc
     2041 acatcctgtc gctgtatcaa acgccacggc gcgccggaca gaccccggga tacaccgaga
     2101 tctccgtcaa tgaatatata aggtctagtt ccacttttaa gcaccgttca aaattacagc
     2161 gctttggctt cgatgggtct gcgggcagtg ccgctgggag tggtcgcgtt gacgtaacgg
     2221 ggtttaaagt tgagcccatt ggcgtggggg attactatgg ctttaccgtc gacggtgatc
     2281 acttgtacct tgatggggat ttcgtcagac atcataattg cgggaagggc gccctgaagg
     2341 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga
     2401 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt
     2461 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg
     2521 gccgccactc caccggcggc atggacgagc tgtacaagca tcatcaccat caccactaat
     2581 aatactagta gcggccgctg caggagtcac taagggttag ttagttagat tagcagaaag
     2641 tcaaaagcct ccgaccggag gcttttgact aaaacttccc ttggggttat cattggggct
     2701 cactcaaagg cggtaatcag ataaaaaaaa tccttagctt tcgctaagga tgatttctgc
     2761 tagagatgga atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     2821 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     2881 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     2941 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     3001 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     3061 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     3121 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccttaataa gatgatcttc
     3181 ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca
     3241 gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg
     3301 aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa
     3361 gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt
     3421 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg
     3481 gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg
     3541 gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca
     3601 ggagagcgca cgagggagcc gccaggggaa acgcctggta tctttatagt cctgtcgggt
     3661 ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat
     3721 ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta agtatcttcc tggcatcttc
     3781 caggaaatct ccgccccgtt cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg
     3841 tagcgagtca gtgagcgagg aagcggaata tatcctgtat cacatattct gctgacgcac
     3901 cggtgcagcc ttttttctcc tgccacatga agcacttcac tgacaccctc atcagtgcca
     3961 acatagtaag ccagtataca ctccgctagc gctgaggtct gcctcgtgaa gaaggtgttg
     4021 ctgactcata ccaggcctga atcgccccat catccagcca gaaagtgagg gagccacggt
     4081 tgatgagagc tttgttgtag gtggaccagt tggtgatttt gaacttttgc tttgccacgg
     4141 aacggtctgc gttgtcggga agatgcgtga tctgatcctt caactcagca aaagttcgat
     4201 ttattcaaca aagccacgtt gtgtctcaaa atctctgatg ttacattgca caagataaaa
     4261 atatatcatc atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgttt
     4321 actagaggag attctcatgt ttgacagctt atcatcgata agctttaatg cggtagttta
     4381 tcacagttaa attgctaacg cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc
     4441 gtcattctcg gcaccgtcac cctggacgct gtaggcatag gcttggttat gccggtactg
     4501 ccgggcctct tgcgggatat cgtccattcc gacagtattg ccagtcacta tggcgtgctg
     4561 cttgcgctct atgcgttgat gcaatttctt tgcgcacccg ttctcggagc cctgtccgac
     4621 cgctttggcc gccgtccagt cctgctcgct tcgctccttg gagccactat cgactacgcg
     4681 atcatggcga ccacacccgt cctgtggatt ctctacgccg gacgcatcgt ggcgggcatc
     4741 acgggtgcca caggtgcggt tgctggtgcc tatatcgccg acatcaccga tggggaagat
     4801 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     4861 gtggccgggg gactgttggg tgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     4921 ctcaacggcc tcaacctcct cctgggctgc ttccttatgc aggaatcgca taagggagag
     4981 cgccgtccga tgcccttgcg tgccttcaat ccagtcagct ccttccggtg ggcgcggggc
     5041 atgactatcg tcgccgcact tatgactgtt ttctttatca tgcaactcgt aggacaggtt
     5101 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc
     5161 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcacgggc
     5221 cccgccacca aacgtttcgg cgagaagcag gccattatcg cgggcatggc ggccgacgcg
     5281 ctgggctacg tcttgctggc gttcgcgacg cgcggctgga tggccttccc cattatgatt
     5341 cttctcgctt ccggcggcat cggtatgccc gcgttgcagg ccatgctgtc ccgccaagta
     5401 gatgacgacc atcagggaca gcttcaaggg tcgctcgcgg ctcttaccag cctcacttcg
     5461 atcattggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg
     5521 ttggcatgga ttgtaggtgc cgccctttac cttgtctgcc tccccgcgtt gcgtcgcggt
     5581 gcatggagcc gggccacctc gacctaataa tactagctcc ggcaaaaaaa cgggcaaggt
     5641 gtcaccaccc tgcccttttt ctttaaaacc gaaaagatta cttcgcgtt
//