LOCUS       pcDNA3-CBX1mut-Dronpa    6378 bp      DNA     circular   12/17/19
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : pcDNA3-Venus-cpVenus-FLARE-AKAR [5506  nt] : (
            
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     CDS             922..939
                     /label=6His
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             1018..1179
                     /label=CBX1mut_20-73
                     /SerialCloner_Color=&h333333
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             1195..1866
                     /label=Dronpa
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(4217..4237)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(1931..1948)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     rep_origin      complement(4605..5233)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_binding    complement(4243..4265)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             complement(5385..6044)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             3086..3877
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     Promoter        complement(6284..6312)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     Promoter        complement(4270..4299)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2867..2944
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     terminator      1971..2198
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     promoter        232..819
                     /label=CMV prom
                     /SerialCloner_Color=&h800080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 
      901 GCCACCATGG TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT 
      961 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGgatcCGGC CACCATGGAA 
     1021 TATGAGGTGG AAGAGGTTCT CGACCGTCGA GTGGTAAAGG GCAAAGTGGA GTACCTCCTA 
     1081 AAGTGGAAGG GATTCTCAGA TGAGGACAAC ACATGGGAGC CAGAAGAGAA CCTGAGTTGT 
     1141 CCCGATCTCA TTGCTGAGTT TCTGCAGTCA CAGAAAACAG GAGGTAGTGg tacCATGAGT 
     1201 GTGATTAAAC CAGACATGAA GATCAAGCTG CGTATGGAAG GCGCTGTAAA TGGACACCCG 
     1261 TTCGCGATTG AAGGAGTTGG CCTTGGGAAG CCTTTCGAGG GAAAACAGAG TATGGACCTT 
     1321 AAAGTCAAAG AAGGCGGACC TCTGCCTTTC GCCTATGACA TCTTGACAAC TGTGTTCTGT 
     1381 TACGGCAACA GGGTATTCGC CAAATACCCA GAAAATATAG TAGACTATTT CAAGCAGTCG 
     1441 TTTCCTGAGG GCTACTCTTG GGAACGAAGC ATGAATTACG AAGACGGGGG CATTTGTAAC 
     1501 GCGACAAACG ACATAACCCT GGATGGTGAC TGTTATATCT ATGAAATTCG ATTTGATGGT 
     1561 GTGAACTTTC CTGCCAATGG TCCAGTTATG CAGAAGAGGA CTGTGAAATG GGAGCCATCC 
     1621 ACTGAGAAAT TGTATGTGCG TGATGGAGTG CTGAAGGGTG ATGTTAACAT GGCTCTGTCG 
     1681 CTTGAAGGAG GTGGCCATTA CCGATGTGAC TTCAAAACTA CTTATAAAGC TAAGAAGGTT 
     1741 GTCCAGTTGC CAGACTATCA CTTTGTGGAC CACCACATTG AGATTAAAAG CCACGACAAA 
     1801 GATTACAGTA ATGTTAATCT GCATGAGCAT GCCGAAGCGC ATTCTGAGCT GCCGAGGCAG 
     1861 GCCAAGTAAG aattCTGCAG ATATCCATCA CACTGGCGGC CGCTCGAGCA TGCATCTAGA 
     1921 GGGCCCTATT ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc 
     1981 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg 
     2041 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag 
     2101 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga 
     2161 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag 
     2221 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 
     2281 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 
     2341 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 
     2401 catcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 
     2461 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 
     2521 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 
     2581 ctcggtctat tcttttgatt tataagggat tttggggatt tcggcctatt ggttaaaaaa 
     2641 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg 
     2701 tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc atctcaatta 
     2761 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 
     2821 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac 
     2881 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 
     2941 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 
     3001 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga 
     3061 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc 
     3121 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 
     3181 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 
     3241 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac 
     3301 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 
     3361 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 
     3421 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 
     3481 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 
     3541 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 
     3601 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 
     3661 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 
     3721 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 
     3781 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 
     3841 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg 
     3901 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat 
     3961 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg 
     4021 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac 
     4081 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 
     4141 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc 
     4201 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca 
     4261 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg 
     4321 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg 
     4381 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc 
     4441 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta 
     4501 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag 
     4561 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg 
     4621 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg 
     4681 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg 
     4741 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga 
     4801 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc 
     4861 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt 
     4921 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact 
     4981 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg 
     5041 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt 
     5101 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt 
     5161 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct 
     5221 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg 
     5281 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt 
     5341 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt 
     5401 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc 
     5461 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg 
     5521 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc 
     5581 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg 
     5641 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca 
     5701 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga 
     5761 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct 
     5821 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg 
     5881 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca 
     5941 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata 
     6001 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct 
     6061 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact 
     6121 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa 
     6181 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc 
     6241 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga 
     6301 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 
     6361 aaagtgccac ctgacgtc
//