LOCUS       pcDNA3-CBX1mut-PA-mCherry    6414 bp      DNA     circular   12/17/19
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=DNA
COMMENT     SerialCloner_Comments=Ligation of : pcDNA3-CBX1mut-Dronpa [5693  nt] : (EcoRI[1870
            
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     CDS             922..939
                     /label=6His
                     /SerialCloner_Color=&h008000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             1018..1179
                     /label=CBX1mut_20-73
                     /SerialCloner_Color=&h333333
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(4253..4273)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(1967..1984)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     rep_origin      complement(4641..5269)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_binding    complement(4279..4301)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             complement(5421..6080)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             3122..3913
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     Promoter        complement(6320..6348)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     Promoter        complement(4306..4335)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    2903..2980
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     terminator      2007..2234
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     promoter        232..819
                     /label=CMV prom
                     /SerialCloner_Color=&h800080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             1195..1902
                     /label=PA-mCherry
                     /SerialCloner_Color=&hFB0106
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAA GCTTGCGGCC 
      901 GCCACCATGG TGCGGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT 
      961 GGACAGCAAA TGGGTCGGGA TCTGTACGAC GATGACGATA AGgatcCGGC CACCATGGAA 
     1021 TATGAGGTGG AAGAGGTTCT CGACCGTCGA GTGGTAAAGG GCAAAGTGGA GTACCTCCTA 
     1081 AAGTGGAAGG GATTCTCAGA TGAGGACAAC ACATGGGAGC CAGAAGAGAA CCTGAGTTGT 
     1141 CCCGATCTCA TTGCTGAGTT TCTGCAGTCA CAGAAAACAG GAGGTAGTGg tacCATGGTG 
     1201 AGCAAGGGCG AGGAGGATAA CATGGCCATC ATTAAGGAGT TCATGCGCTT CAAGGTGCAC 
     1261 ATGGAGGGGT CCGTGAACGG CCACGTGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC 
     1321 TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG GTGGCCCCCT GCCCTTCACC 
     1381 TGGGACATCC TGTCCCCTCA ATTCATGTAC GGCTCCAATG CCTACGTGAA GCACCCCGCC 
     1441 GACATCCCCG ACTACTTTAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG 
     1501 AAATTCGAGG ACGGCGGCGT GGTGACCGTG ACCCAGGACT CCTCCCTGCA GGACGGTGAG 
     1561 TTCATCTACA AGGTGAAGCT GCGCGGCACC AACTTCCCCT CCGACGGCCC CGTAATGCAG 
     1621 AAGAAGACCA TGGGCTGGGA GGCCCTCTCC GAGCGGATGT ACCCCGAGGA CGGCGCCCTG 
     1681 AAGGGCGAGG TCAAGCCGAG AGTGAAGCTG AAGGACGGCG GCCACTACGA CGCTGAGGTC 
     1741 AAGACCACCT ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG GCGCCTACAA CGTCAACCGC 
     1801 AAGTTGGACA TCACCTCACA CAACGAGGAC TACACCATCG TGGAACAGTA CGAACGTGCC 
     1861 GAGGGCCGCC ACTCCACCGG CGGCATGGAC GAGCTGTACA AGTAAGaatt CTGCAGATAT 
     1921 CCATCACACT GGCGGCCGCT CGAGCATGCA TCTAGAGGGC CCTATTctat agtgtcacct 
     1981 aaatgctaga gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt 
     2041 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta 
     2101 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg 
     2161 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc 
     2221 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca 
     2281 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc 
     2341 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac 
     2401 gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt tccgatttag 
     2461 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc 
     2521 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg 
     2581 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata 
     2641 agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa 
     2701 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 
     2761 ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 
     2821 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 
     2881 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 
     2941 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 
     3001 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 
     3061 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat 
     3121 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 
     3181 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 
     3241 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 
     3301 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 
     3361 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 
     3421 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 
     3481 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 
     3541 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 
     3601 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 
     3661 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 
     3721 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 
     3781 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 
     3841 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 
     3901 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg 
     3961 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt 
     4021 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc 
     4081 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 
     4141 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 
     4201 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca 
     4261 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 
     4321 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 
     4381 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 
     4441 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 
     4501 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 
     4561 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 
     4621 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 
     4681 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 
     4741 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 
     4801 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca 
     4861 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 
     4921 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 
     4981 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 
     5041 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 
     5101 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 
     5161 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 
     5221 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 
     5281 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 
     5341 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 
     5401 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 
     5461 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 
     5521 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 
     5581 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 
     5641 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 
     5701 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 
     5761 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 
     5821 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 
     5881 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 
     5941 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 
     6001 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 
     6061 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 
     6121 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 
     6181 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 
     6241 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 
     6301 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 
     6361 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
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