LOCUS       pcDNA3-FKBP-ICUE3-NLS    9468 bp      DNA     circular   3/13/19
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     Serial Cloner Genbank Format
COMMENT     SerialCloner_Type=Degen. DNA
COMMENT     SerialCloner_Comments=Ligation of : pcDNA3-ICUE3-NLS.gb.xdna [9059  nt] : (BamHI[1
            002] / HindIII[889]) to FKBP-PARE(CMV_F).xdna [400  nt] : (H
            indIII[94] / BamHI[498])
COMMENT     SerialCloner_Ends=0,0,,0,
FEATURES             Location/Qualifiers
     CDS             1300..2016
                     /label=full-mECFP
                     /SerialCloner_Color=&h66CCFF
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             4231..4965
                     /label=cpVenus L194
                     /SerialCloner_Color=&hFFFF00
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             2026..4224
                     /label=Epac1_149-881
                     /SerialCloner_Color=&hFF6666
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(7307..7327)
                     /label=M13-rev
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     863..882
                     /label=T7
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     primer_bind     complement(5021..5038)
                     /label=SP6
                     /SerialCloner_Color=&h008B8B
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     rep_origin      complement(7695..8323)
                     /label=ColE1 origin
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_binding    complement(7333..7355)
                     /label=LacO
                     /SerialCloner_Color=&h6495ED
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             complement(8475..9134)
                     /label=AmpR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             6176..6967
                     /label=Kan/neoR
                     /SerialCloner_Color=&h8FBC8F
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     Promoter        complement(9374..9402)
                     /label=Amp prom
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     Promoter        complement(7360..7389)
                     /label=lac
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    5957..6034
                     /label=SV40 ORI
                     /SerialCloner_Color=&h4682B4
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     terminator      5061..5288
                     /label=bGH PA
                     /SerialCloner_Color=&hFF8000
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     promoter        232..819
                     /label=CMV prom
                     /SerialCloner_Color=&h800080
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     misc_feature    4972..5001
                     /label=NLS-Targeting
                     /SerialCloner_Color=&h800040
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=True
                     /SerialCloner_Arrow=True
     CDS             907..1230
                     /label=FKBP Domain
                     /SerialCloner_Color=&h84A4C0
                     /SerialCloner_Show=True
                     /SerialCloner_Protect=False
                     /SerialCloner_Arrow=True
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggAGACCCAa gctTGCGGCC 
      901 GCCACCATGG GAGTGCAGGT GGAAACCATC TCCCCAGGAG ACGGGCGCAC CTTCCCCAAG 
      961 CGCGGCCAGA CCTGCGTGGT GCACTACACC GGGATGCTTG AAGATGGAAA GAAATTTGAT 
     1021 TCCTCCCGGG ACAGAAACAA GCCCTTTAAG TTTATGCTAG GCAAGCAGGA GGTGATCCGA 
     1081 GGCTGGGAAG AAGGGGTTGC CCAGATGAGT GTGGGTCAGA GAGCCAAACT GACTATATCT 
     1141 CCAGATTATG CCTATGGTGC CACTGGGCAC CCAGGCATCA TCCCACCACA TGCCACTCTC 
     1201 GTCTTCGATG TGGAGCTTCT AAAACTGGAA AGTGCTGGTG GTAGTGCTGG TGGTAGTGCT 
     1261 GGTGGTAGTG CTGGTGGTAG TGCTGGTGGT GGGgatccCA TGGTGAGCAA GGGCGAGGAG 
     1321 CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAGG 
     1381 TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGTTC 
     1441 ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGACCTGG 
     1501 GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC 
     1561 GCCATGCCCG AAGGCTACGT CCAGGAGCGT ACCATCTTCT TCAAGGACGA CGGCAACTAC 
     1621 AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG 
     1681 GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACATC 
     1741 AGCCACAACG TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CCACTTCAAG 
     1801 ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC 
     1861 CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCAAG 
     1921 CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC 
     1981 GCCGGGATCA CTCACGGCAT GGACGAGCTG TACAAGGCTG GTACCcccgt gggaactcat 
     2041 gagatggagg aggagttggc cgaagctgtg gccctgctct cccagcgggg gcctgacgcc 
     2101 ctgctcactg tggcacttcg aaagccccca ggtcagcgca cggatgaaga gctggacctc 
     2161 atctttgagg agctgctgca catcaaggct gtggcccacc tctccaactc ggtgaagcga 
     2221 gaattagcgg ctgttctgct ctttgaacca cacagcaagg cagggaccgt gttgttcagc 
     2281 cagggggaca agggcacttc gtggtacatt atctggaagg gatctgtcaa cgtggtgacc 
     2341 catggcaagg ggctggtgac caccctgcat gagggagatg attttggaca gctggctctg 
     2401 gtgaatgatg caccccgggc agccaccatc atcctgcgag aagacaactg tcatttcctg 
     2461 cgtgtggaca agcaggactt caaccgtatc atcaaggatg tggaggcaaa gaccatgcgg 
     2521 ctggaagaac atggcaaagt ggtgctggtg ctggagagag cctctcaggg cgccggccct 
     2581 tcccgacccc caaccccagg caggaaccgg tatacagtga tgtctggcac cccagagaag 
     2641 atcctagagc ttctgttgga ggccatggga ccagattcca gtgctcatga ccaaacagag 
     2701 acattcctca gcgacttcct cctgacccac agggtcttca tgcccagcgc ccaactctgc 
     2761 gctgcccttc tgcaccactt ccatgtggag cctgcgggtg gcagcgagca ggagcgcagc 
     2821 acctacgtct gcaacaagag gcagcagatc ttgcggctgg tcagccagtg ggtggccctg 
     2881 tatggctcca tgctccacac tgaccctgtg gccaccagct tcctccagaa actctcagac 
     2941 ctggtgggca gggacacccg actcagcaac ctgctgaggg agcagtggcc agagaggcgg 
     3001 cgatgccaca ggttggagaa tggctgtggg aatgcatctc ctcagatgaa ggcccggaac 
     3061 ttgcctgttt ggctccccaa ccaggacgag ccccttcctg gcagcagctg tgccatccaa 
     3121 gttggggata aagtccccta tgacatctgc cggccagacc actcagtgtt gaccctgcag 
     3181 ctgcctgtga cagcctccgt gagagaggtg atggcagcgt tggcccagga ggatggctgg 
     3241 accaaggggc aggtgctggt gaaggtcaat tctgcaggtg atgccattgg cctgcagcca 
     3301 gatgcccgtg gtgtggccac atctctgggg ctcaatgagc gtctctttgt tgtcaaccca 
     3361 caggaagcgc atgagctgat cccacaccct gaccagctgg ggcccactgt gggctctgct 
     3421 gaggggctgg acctggtgag tgccaaggac ctggcaggcc agctgacgga ccacgactgg 
     3481 agcctcttca acagtatcca ccaggtggag ctgatccact atgtgctggg cccccagcat 
     3541 ctgcgggatg tcaccaccgc caacctggag cgcttcatgc gccgcttcaa tgagctgcag 
     3601 tactgggtgg ccaccgagct gtgtctctgc cccgtgcccg gcccccgggc ccagctgctc 
     3661 aggaagttca ttaagctggc ggcccacctc aaggagcaga agaatctcaa ttccttcttt 
     3721 gccgtcatgt ttggcctcag caactcggcc atcagccacc tagcccacac ctgggagcgg 
     3781 ctgccccaca aagtccggaa gctgtactcc gccctcgaga ggctgctgga cccctcatgg 
     3841 aaccaccggg tataccgact ggccctcgcc aagctctccc ctcctgtcat ccccttcatg 
     3901 ccccttcttc tcaaagacat gaccttcatt catgagggaa accacacact agtggagaat 
     3961 ctcatcaact ttgagaagat gagaatgatg gccagagtcg cgcggatgct gcaccactgc 
     4021 cgaagccaca accctgtgcc tctctcacca ctcagaagcc gagtttccca cctccacgag 
     4081 gacagccagg tggcgaggat ttccacatgc tcggagcagt ccctgagcac ccggagtcca 
     4141 gccagcacct gggcttatgt ccagcagctg aaggtcattg acaaccagcg ggaactctcc 
     4201 cgcctctccc gagagctgga gccaGAGCTC ATGCCCGACA ACCACTACCT GAGCTACCAG 
     4261 TCCAAGCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG 
     4321 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGGGAGGTAC CGGTGGATCT 
     4381 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC 
     4441 GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC 
     4501 GGCAAGCTGA CCCTGAAGCT GATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC 
     4561 CTCGTGACCA CCCTGGGCTA CGGCCTTCAG TGCTTCGCCC GCTACCCCGA CCACATGAAG 
     4621 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC 
     4681 TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG 
     4741 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC 
     4801 AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA CCGCCGACAA GCAGAAGAAC 
     4861 GGCATCAAGG CCAACTTCAA GATCCGCCAC AACATCGAGG ACGGCGGCGT GCAGCTCGCC 
     4921 GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGGAATT CCCCAAAAAG 
     4981 AAGAGAAAGG TGGAAGACGC ATAATCTAGA GGGCCCTATT ctatagtgtc acctaaatgc 
     5041 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc 
     5101 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa 
     5161 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg 
     5221 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg 
     5281 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc 
     5341 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact 
     5401 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc 
     5461 cggctttccc cgtcaagctc taaatcgggg catcccttta gggttccgat ttagtgcttt 
     5521 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc 
     5581 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt 
     5641 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat 
     5701 tttggggatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa 
     5761 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccaggcagg 
     5821 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 
     5881 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 
     5941 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 
     6001 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt 
     6061 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc 
     6121 ttgtatatcc attttcggat ctgatcaaga gacaggatga ggatcgtttc gcatgattga 
     6181 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 
     6241 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 
     6301 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga 
     6361 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 
     6421 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 
     6481 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 
     6541 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 
     6601 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 
     6661 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga 
     6721 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 
     6781 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 
     6841 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 
     6901 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 
     6961 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca 
     7021 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg 
     7081 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc 
     7141 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 
     7201 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 
     7261 tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg 
     7321 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata 
     7381 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca 
     7441 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc 
     7501 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg 
     7561 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 
     7621 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 
     7681 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 
     7741 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 
     7801 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 
     7861 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt 
     7921 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 
     7981 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 
     8041 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 
     8101 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 
     8161 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 
     8221 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 
     8281 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 
     8341 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 
     8401 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 
     8461 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 
     8521 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 
     8581 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 
     8641 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 
     8701 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 
     8761 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt 
     8821 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 
     8881 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 
     8941 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 
     9001 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 
     9061 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact 
     9121 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 
     9181 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 
     9241 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 
     9301 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 
     9361 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 
     9421 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//