LOCUS       pYY-538_(pYY-BEM3.14_F130L)        8826 bp    DNA     circular UNA 27-NOV-2019
DEFINITION  .
ACCESSION   urn.local...8n-b8yzjf7
VERSION     urn.local...8n-b8yzjf7
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        35..611
                     /created_by="User"
                     /modified_by="User"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS,pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS,pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS,pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS,pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1(1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B16-ScCas9D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS,pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS ,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS,pYY-B19-Flag-TadA-TadA7.10
                     *-NLS , pYY-B2-SpCas9 D10A-NLS,pYY-B2-SpCas9 D10A-NLS ,
                     pYY-B20-flag-rAPOBEC1-NLS,pYY-B20-flag-rAPOBEC1-NLS ,
                     pYY-B21(flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4(rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1)
                     ,pYY-B23-FLAG-ppBE4 (rAPOBEC1), pYY-B3-SpCas9
                     D10AH840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B4-ScCas9D10A-NLS, pYY-B4-ScCas9 D10A-NLS ,
                     pYY-B5-Sp-ScCas9D10A-NLS, pYY-B5-Sp-ScCas9 D10A-NLS
                     ,pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS
                     ,pYY-B7-SpCas9 D10A-UGI-UGI-NLS,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS ,
                     pYY-B9mutation-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS,pYY-B9-rAPOBEC1-NLS ,
                     pYY-BG34-rAPOBEC1 W90Y R126E-NLS,pYY-BG35 rAPOBEC1 W90Y
                     R126E R132E, pYY-BGX13-hA3A-SpCas9D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl,pYY-BGX27-pmCDA1-SpCas9
                     D10A-UGl, pYY-BGX29-pmCDA5-SpCas9D10A-UGI,
                     pYY-BGX34-rAPOBEC1 W90Y R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BGn-deaminase-NLSCopy, pYY-BX1-pmCDA-SpCas9
                     D10A-UGl,pYY-BX2-TadA-TadA7.10-SpCas9 D10A,
                     pYY-BX3-TadA7.10-SpCas9D10A, pYY-BX4-A3A-SpCas9 D10A-UGl ,
                     pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="pCMV immearly protomter"
     misc_feature    114..282
                     /created_by="User"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS,pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS,pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS,pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS,pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1(1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B16-ScCas9D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS,pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS ,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS,pYY-B19-Flag-TadA-TadA7.10
                     *-NLS , pYY-B2-SpCas9 D10A-NLS,pYY-B2-SpCas9 D10A-NLS ,
                     pYY-B20-flag-rAPOBEC1-NLS,pYY-B20-flag-rAPOBEC1-NLS ,
                     pYY-B21(flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4(rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1)
                     ,pYY-B23-FLAG-ppBE4 (rAPOBEC1), pYY-B3-SpCas9
                     D10AH840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B4-ScCas9D10A-NLS, pYY-B4-ScCas9 D10A-NLS ,
                     pYY-B5-Sp-ScCas9D10A-NLS, pYY-B5-Sp-ScCas9 D10A-NLS
                     ,pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS
                     ,pYY-B7-SpCas9 D10A-UGI-UGI-NLS,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS ,
                     pYY-B9mutation-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS,pYY-B9-rAPOBEC1-NLS ,
                     pYY-BG34-rAPOBEC1 W90Y R126E-NLS,pYY-BG35 rAPOBEC1 W90Y
                     R126E R132E, pYY-BGX13-hA3A-SpCas9D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl,pYY-BGX27-pmCDA1-SpCas9
                     D10A-UGl, pYY-BGX29-pmCDA5-SpCas9D10A-UGI,
                     pYY-BGX34-rAPOBEC1 W90Y R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BGn-deaminase-NLSCopy, pYY-BX1-pmCDA-SpCas9
                     D10A-UGl,pYY-BX2-TadA-TadA7.10-SpCas9 D10A,
                     pYY-BX3-TadA7.10-SpCas9D10A, pYY-BX4-A3A-SpCas9 D10A-UGl ,
                     pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="CAG enhancer"
     primer_bind     294..326
                     /Sequence="ACGCCCCCTATTGACGTCAATGACGGTAAATGG"
                     /Hairpin_Tm="56.5"
                     /Tm="71.1"
                     /Self_Dimer_Tm="30.9"
                     /%GC="51.5"
                     /created_by="primer3"
                     /Transferred_From="pYY-AS22 BE4 Y120A"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-839"
     misc_feature    437..448
                     /Recognition_pattern="(11/13)CAANNNNNGTGG(12/10)"
                     /Transferred_From="pYY-B11-TadA7.10*-NLS,
                     pYY-B11-TadA7.10*-NLS , pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9 D10A-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="CspCI"
                     /note="Geneious type: restriction site"
     promoter        569..638
                     /created_by="User"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS,pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS,pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS,pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS,pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1(1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B16-ScCas9D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS,pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS ,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS,pYY-B19-Flag-TadA-TadA7.10
                     *-NLS , pYY-B2-SpCas9 D10A-NLS,pYY-B2-SpCas9 D10A-NLS ,
                     pYY-B20-flag-rAPOBEC1-NLS,pYY-B20-flag-rAPOBEC1-NLS ,
                     pYY-B21(flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4(rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1)
                     ,pYY-B23-FLAG-ppBE4 (rAPOBEC1), pYY-B3-SpCas9
                     D10AH840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B4-ScCas9D10A-NLS, pYY-B4-ScCas9 D10A-NLS ,
                     pYY-B5-Sp-ScCas9D10A-NLS, pYY-B5-Sp-ScCas9 D10A-NLS
                     ,pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS
                     ,pYY-B7-SpCas9 D10A-UGI-UGI-NLS,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS ,
                     pYY-B9mutation-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS,pYY-B9-rAPOBEC1-NLS ,
                     pYY-BG34-rAPOBEC1 W90Y R126E-NLS,pYY-BG35 rAPOBEC1 W90Y
                     R126E R132E, pYY-BGX13-hA3A-SpCas9D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl,pYY-BGX27-pmCDA1-SpCas9
                     D10A-UGl, pYY-BGX29-pmCDA5-SpCas9D10A-UGI,
                     pYY-BGX34-rAPOBEC1 W90Y R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BGn-deaminase-NLSCopy, pYY-BX1-pmCDA-SpCas9
                     D10A-UGl,pYY-BX2-TadA-TadA7.10-SpCas9 D10A,
                     pYY-BX3-TadA7.10-SpCas9D10A, pYY-BX4-A3A-SpCas9 D10A-UGl ,
                     pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="CMV promoter"
     misc_feature    complement(642..659)
                     /Sequence="AGCGGCCGCGGATCTCTA"
                     /Hairpin_Tm="47.3"
                     /Tm="63.3"
                     /Self_Dimer_Tm="41.7"
                     /%GC="66.7"
                     /created_by="primer3"
                     /Transferred_From="pYY-B12-pmCDA-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-837"
                     /note="Geneious type: primer_bind_reverse"
     Primer          643..662
                     /created_by="yiyu"
                     /Transferred_From="pYY-BGn-deaminase-NLS Copy"
                     /Transferred_Similarity="100.00%"
                     /label="28 B insert FP"
     Primer          complement(643..662)
                     /created_by="yiyu"
                     /modified_by="yiyu"
                     /Transferred_From="pYY-BGn-deaminase-NLS Copy"
                     /Transferred_Similarity="100.00%"
                     /label="29 B BB RP"
     unsure          645..>658
                     /created_by="User"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-BGX13-hA3A-SpCas9
                     D10A-UGl,pYY-BGX26-mAID-SpCas9 D10A-UGl,
                     pYY-BGX27-pmCDA1-SpCas9D10A-UGl, pYY-BGX29-pmCDA5-SpCas9
                     D10A-UGI,pYY-BGX34-rAPOBEC1 W90Y
                     R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX2-TadA-TadA7.10-SpCas9
                     D10A,pYY-BX3-TadA7.10-SpCas9 D10A, pYY-BX4-A3A-SpCas9
                     D10A-UGl, pYY-CMn chimeric APOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="USER"
     unsure          645..659
                     /created_by="User"
                     /Transferred_From="pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS, pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS, pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS, pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS, pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1 (1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B17 SaCas9 D10A-NLS, pYY-B18-SaCas9
                     D10A-UGI-UGI-NLS, pYY-B18-SaCas9 D10A-UGI-UGI-NLS ,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS , pYY-B2-SpCas9 D10A-NLS,
                     pYY-B2-SpCas9 D10A-NLS , pYY-B20-flag-rAPOBEC1-NLS,
                     pYY-B20-flag-rAPOBEC1-NLS , pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A), pYY-B22-FLAG-BE4
                     (rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1) ,
                     pYY-B23-FLAG-ppBE4 (rAPOBEC1), pYY-B3-SpCas9 D10A
                     H840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS ,
                     pYY-B7-SpCas9 D10A-UGI-UGI-NLS, pYY-B7-SpCas9
                     D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS,
                     pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS , pYY-B9
                     mutation-rAPOBEC1-NLS, pYY-B9-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS , pYY-BG34-rAPOBEC1 W90Y R126E-NLS,
                     pYY-BG35 rAPOBEC1 W90Y R126E R132E, pYY-BGn-deaminase-NLS
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /label="USER"
     primer_bind     645..664
                     /Sequence="AGATCCGCGGCCGCTAATAC"
                     /Hairpin_Tm="39.3"
                     /Tm="62.5"
                     /Self_Dimer_Tm="27.0"
                     /%GC="60.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B12-pmCDA-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-836"
     primer_bind     651..677
                     /Sequence="GCGGCCGCTAATACGACTCACTATAGG"
                     /Hairpin_Tm="38.3"
                     /Tm="66.5"
                     /Self_Dimer_Tm="41.7"
                     /%GC="55.6"
                     /modified_by="yiyu"
                     /created_by="primer3"
                     /Transferred_From="pYY-AS22 BE4 Y120A,
                     pYY-BX2-TadA-TadA7.10-SpCas9 D10A, pYY-BX3-TadA7.10-SpCas9
                     D10A"
                     /Transferred_Similarity="100.00%"
                     /label="18 BX1 FP; 21 NotI pmCDA FP; 25 BX3 FP NotI"
     primer_bind     651..675
                     /Sequence="GCGGCCGCTAATACGACTCACTATA"
                     /Hairpin_Tm="38.3"
                     /Tm="64.3"
                     /Self_Dimer_Tm="41.7"
                     /%GC="52.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-BX4-A3A-SpCas9 D10A-UGl"
                     /Transferred_Similarity="100.00%"
                     /label="26 BX4 FP"
     promoter        659..677
                     /created_by="User"
                     /modified_by="yiyu"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS,pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS,pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS,pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS,pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1(1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B16-ScCas9D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS,pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS ,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS,pYY-B19-Flag-TadA-TadA7.10
                     *-NLS , pYY-B2-SpCas9 D10A-NLS,pYY-B2-SpCas9 D10A-NLS ,
                     pYY-B20-flag-rAPOBEC1-NLS,pYY-B20-flag-rAPOBEC1-NLS ,
                     pYY-B21(flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4(rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1)
                     ,pYY-B23-FLAG-ppBE4 (rAPOBEC1), pYY-B3-SpCas9
                     D10AH840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B4-ScCas9D10A-NLS, pYY-B4-ScCas9 D10A-NLS ,
                     pYY-B5-Sp-ScCas9D10A-NLS, pYY-B5-Sp-ScCas9 D10A-NLS
                     ,pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS
                     ,pYY-B7-SpCas9 D10A-UGI-UGI-NLS,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS ,
                     pYY-B9mutation-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS,pYY-B9-rAPOBEC1-NLS ,
                     pYY-BG34-rAPOBEC1 W90Y R126E-NLS,pYY-BG35 rAPOBEC1 W90Y
                     R126E R132E, pYY-BGX13-hA3A-SpCas9D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl,pYY-BGX27-pmCDA1-SpCas9
                     D10A-UGl, pYY-BGX29-pmCDA5-SpCas9D10A-UGI,
                     pYY-BGX34-rAPOBEC1 W90Y R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BGn-deaminase-NLSCopy, pYY-BX1-pmCDA-SpCas9
                     D10A-UGl,pYY-BX2-TadA-TadA7.10-SpCas9 D10A,
                     pYY-BX3-TadA7.10-SpCas9D10A, pYY-BX4-A3A-SpCas9 D10A-UGl ,
                     pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="T7 promoter; T7 protomet"
     primer_bind     659..678
                     /Sequence="TAATACGACTCACTATAGGG"
                     /Hairpin_Tm="None"
                     /Tm="50.3"
                     /Self_Dimer_Tm="None"
                     /%GC="40.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-AS22 BE4 Y120A"
                     /Transferred_Similarity="100.00%"
                     /label="T7"
     primer_bind     complement(669..690)
                     /Sequence="GGTGGCGGCTCTCCCTATAGTG"
                     /Hairpin_Tm="None"
                     /Tm="64.2"
                     /Self_Dimer_Tm="11.4"
                     /%GC="63.6"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A), pYY-B23-FLAG-ppBE4
                     (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="54 B21 RP; B21 RP"
     Target_site     complement(672..690)
                     /created_by="yiyu"
                     /modified_by="yiyu"
                     /Transferred_From="pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS, pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS, pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS, pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS, pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1 (1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B19-Flag-TadA-TadA7.10*-NLS,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS , pYY-B2-SpCas9 D10A-NLS,
                     pYY-B2-SpCas9 D10A-NLS , pYY-B20-flag-rAPOBEC1-NLS,
                     pYY-B20-flag-rAPOBEC1-NLS , pYY-B3-SpCas9 D10A H840A-NLS,
                     pYY-B3-SpCas9 D10A H840A-NLS , pYY-B9
                     mutation-rAPOBEC1-NLS, pYY-B9-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS , pYY-BG34-rAPOBEC1 W90Y R126E-NLS,
                     pYY-BG35 rAPOBEC1 W90Y R126E R132E, pYY-BGn-deaminase-NLS
                     Copy, pYY-BX4-A3A-SpCas9 D10A-UGl"
                     /Transferred_Similarity="100.00%"
                     /label="1 pCMV BB RP"
     primer_bind     complement(672..690)
                     /Mismatches=0
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="11.4"
                     /Tm="60.5"
                     /Sequence="GGTGGCGGCTCTCCCTATA"
                     /created_by="primer3"
                     /Transferred_From="pYY-B18-SaCas9 D10A-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="1 pCMV BB RP"
     CDS             1273..1368
                     /created_by="ngaudelli"
                     /created_by="ngaudelli"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B22-FLAG-BE4 (rAPOBEC1),
                     pYY-B22-FLAG-BE4(rAPOBEC1) , pYY-B23-FLAG-ppBE4
                     (rAPOBEC1),pYY-BGX13-hA3A-SpCas9 D10A-UGl,
                     pYY-BGX26-mAID-SpCas9D10A-UGl, pYY-BGX27-pmCDA1-SpCas9
                     D10A-UGl,pYY-BGX29-pmCDA5-SpCas9 D10A-UGI,
                     pYY-BGX34-rAPOBEC1 W90YR126E-UGl, pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl ,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX2-TadA-TadA7.10-SpCas9
                     D10A,pYY-BX3-TadA7.10-SpCas9 D10A, pYY-BX4-A3A-SpCas9
                     D10A-UGl, pYY-CMn chimeric APOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="linker"
     CDS             1273..>1284
                     /created_by="ngaudelli"
                     /Transferred_From="pYY-BGn-deaminase-NLS Copy"
                     /Transferred_Similarity="100.00%"
                     /label="linker"
     primer_bind     complement(1346..1390)
                     /Sequence="GGCCGATGCTGTACTTCTTGTCAGAGCCACCAGAAGATCCGCCGC"
                     /created_by="yiyu"
                     /Transferred_From="pYY-B23-FLAG-ppBE4 (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="57 B22 insert RP"
     misc_feature    complement(1347..1366)
                     /Sequence="AGCCACCAGAAGATCCGCCG"
                     /Hairpin_Tm="None"
                     /Tm="64.7"
                     /Self_Dimer_Tm="None"
                     /%GC="65.0"
                     /created_by="primer3"
                     /modified_by="ngaudelli"
                     /Transferred_From="pYY-AS22 BE4 Y120A"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B140"
                     /note="Geneious type: primer_bind_reverse"
     unsure          1353..1366
                     /created_by="ngaudelli"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-BGX13-hA3A-SpCas9
                     D10A-UGl,pYY-BGX26-mAID-SpCas9 D10A-UGl,
                     pYY-BGX27-pmCDA1-SpCas9D10A-UGl, pYY-BGX29-pmCDA5-SpCas9
                     D10A-UGI,pYY-BGX34-rAPOBEC1 W90Y
                     R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl ,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX2-TadA-TadA7.10-SpCas9
                     D10A,pYY-BX3-TadA7.10-SpCas9 D10A, pYY-BX4-A3A-SpCas9
                     D10A-UGl, pYY-CMn chimeric APOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="USER"
     primer_bind     1353..1389
                     /Sequence="ATCTTCTGGTGGCTCTGACAAGAAGTACAGCATCGGC"
                     /created_by="nicolegaudelli"
                     /Transferred_From="pYY-AS22 BE4 Y120A"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B235"
     unsure          1353..1366
                     /created_by="ngaudelli"
                     /Transferred_From="pYY-B22-FLAG-BE4 (rAPOBEC1),
                     pYY-B22-FLAG-BE4 (rAPOBEC1) , pYY-B23-FLAG-ppBE4
                     (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="USER"
     CDS             1369..5469
                     /note="hSpCas9"
                     /modified_by="jonlevy"
                     /modified_by="jonlevy"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A),pYY-BGX13-hA3A-SpCas9
                     D10A-UGl, pYY-BGX26-mAID-SpCas9D10A-UGl,
                     pYY-BGX27-pmCDA1-SpCas9 D10A-UGl,pYY-BGX29-pmCDA5-SpCas9
                     D10A-UGI, pYY-BGX34-rAPOBEC1 W90YR126E-UGl,
                     pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX4-A3A-SpCas9 D10A-UGl
                     , pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="fz hSpCas9"
     primer_bind     1369..1390
                     /Sequence="GACAAGAAGTACAGCATCGGCC"
                     /Hairpin_Tm="42.6"
                     /Tm="61.6"
                     /Self_Dimer_Tm="None"
                     /%GC="54.5"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A), pYY-B23-FLAG-ppBE4
                     (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="55 B21 FP; 55 b21 FP"
     USER            <1369..>1378
                     /created_by="Yi Yu"
                     /modified_by="Yi Yu"
                     /Transferred_From="pYY-B16-ScCas9 D10A R211K, N404K-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="USER1-A03"
     Primer          complement(<1369..>1378)
                     /created_by="Yi Yu"
                     /modified_by="Yi Yu"
                     /Transferred_From="pYY-B16-ScCas9 D10A R211K, N404K-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="YY-AP7 A03 BB RP"
     Primer          <1369..1390
                     /created_by="Yi Yu"
                     /Transferred_From="pYY-B16-ScCas9 D10A R211K, N404K-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="YY-AP8 hSpCas9 FP"
     primer_bind     1370..1395
                     /Sequence="ACAAGAAGTACAGCATCGGCCTGGCC"
                     /Hairpin_Tm="46.8"
                     /Tm="68.9"
                     /Self_Dimer_Tm="28.2"
                     /%GC="57.7"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="KZ495"
     USER            1381..1391
                     /created_by="nicolegaudelli"
                     /Transferred_From="pYY-B1-SpCas9-NLS, pYY-B2-SpCas9
                     D10A-NLS, pYY-B2-SpCas9 D10A-NLS , pYY-B3-SpCas9 D10A
                     H840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS ,
                     pYY-B7-SpCas9 D10A-UGI-UGI-NLS, pYY-B7-SpCas9
                     D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS,
                     pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
     primer_bind     complement(1446..1465)
                     /Sequence="CCTTGAATTTCTTGCTGGGC"
                     /Hairpin_Tm="None"
                     /Tm="57.6"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl"
                     /Transferred_Similarity="100.00%"
                     /label="SP62"
     primer_bind     1490..1510
                     /Sequence="GCATCAAGAAGAACCTGATCG"
                     /Hairpin_Tm="None"
                     /Tm="57.1"
                     /Self_Dimer_Tm="None"
                     /%GC="47.6"
                     /created_by="primer3"
                     /X-Transferred_From="LCv2_seq10"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A), pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq10; NMG-B23"
     misc_feature    complement(1490..1510)
                     /Sequence="CGATCAGGTTCTTCTTGATGC"
                     /Hairpin_Tm="41.8"
                     /Tm="57.1"
                     /Self_Dimer_Tm="None"
                     /%GC="47.6"
                     /created_by="primer3"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B272"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     1518..1537
                     /Sequence="GCTGTTCGACAGCGGCGAAA"
                     /Hairpin_Tm="58.0"
                     /Tm="64.2"
                     /Self_Dimer_Tm="18.3"
                     /%GC="60.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="A03-Seq2000"
     Primer          1616..1637
                     /created_by="yiyu"
                     /modified_by="yiyu"
                     /Transferred_From="pYY-B16-ScCas9 D10A R211K, N404K-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="105 G7 BglII FP"
     Primer          complement(1622..1641)
                     /created_by="yiyu"
                     /modified_by="yiyu"
                     /Transferred_From="pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl"
                     /Transferred_Similarity="100.00%"
                     /label="45 BGYn"
     misc_feature    1659..1669
                     /Recognition_pattern="CCANNNN^NTGG"
                     /annotation_group="CCANNNNNTGG"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS, pYY-B2-SpCas9
                     D10A-NLS,pYY-B2-SpCas9 D10A-NLS , pYY-B3-SpCas9 D10A
                     H840A-NLS,pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B6-SpCas9-UGI-UGI-NLS,pYY-B6-SpCas9-UGI-UGI-NLS ,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS, pYY-B7-SpCas9
                     D10A-UGI-UGI-NLS ,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS,
                     pYY-B8-SpCas9 D10AH840-UGI-UGI-NLS , pYY-BGX13-hA3A-SpCas9
                     D10A-UGl,pYY-BGX26-mAID-SpCas9 D10A-UGl,
                     pYY-BGX27-pmCDA1-SpCas9D10A-UGl, pYY-BGX29-pmCDA5-SpCas9
                     D10A-UGI,pYY-BGX34-rAPOBEC1 W90Y
                     R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX2-TadA-TadA7.10-SpCas9
                     D10A,pYY-BX3-TadA7.10-SpCas9 D10A, pYY-BX4-A3A-SpCas9
                     D10A-UGl, pYY-CMn chimeric APOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="PflMI"
                     /note="Geneious type: restriction site"
     misc_feature    1659..1669
                     /Recognition_pattern="CCANNNN^NTGG"
                     /Transferred_From="pYY-B16-ScCas9 D10A R211K, N404K-NLS,
                     pYY-B21 (flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4 (rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1) ,
                     pYY-B23-FLAG-ppBE4 (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="PflMI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(1796..1815)
                     /Sequence="GTCGGCCTTGTCGGTGCTGT"
                     /Hairpin_Tm="34.3"
                     /Tm="65.4"
                     /Self_Dimer_Tm="None"
                     /%GC="65.0"
                     /created_by="primer3"
                     /modified_by="nicolegaudelli"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B290"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     1796..1815
                     /Sequence="ACAGCACCGACAAGGCCGAC"
                     /Hairpin_Tm="46.5"
                     /Tm="65.4"
                     /Self_Dimer_Tm="None"
                     /%GC="65.0"
                     /modified_by="nicolegaudelli"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B289"
     primer_bind     1942..1961
                     /Sequence="AACCAGCTGTTCGAGGAAAA"
                     /Hairpin_Tm="28.5"
                     /Tm="57.7"
                     /Self_Dimer_Tm="13.8"
                     /%GC="45.0"
                     /created_by="primer3"
                     /X-Transferred_From="LCv2_seq11"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A), pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq11; NMG-B24"
     primer_bind     2018..2037
                     /Sequence="AGAGCAGACGGCTGGAAAAT"
                     /Hairpin_Tm="57.6"
                     /Tm="59.7"
                     /Self_Dimer_Tm="22.3"
                     /%GC="50.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="A03-Seq2500; A03-Seq2500"
     primer_bind     2494..2513
                     /Sequence="AAGCCCATCCTGGAAAAGAT"
                     /Transferred_From="LCv2_seq12"
                     /Transferred_Similarity="100.00%"
                     /X-Transferred_From="LCv2_seq12"
                     /X-Transferred_Similarity="100.00%"
                     /Hairpin_Tm="41.0"
                     /Tm="56.8"
                     /Self_Dimer_Tm="None"
                     /%GC="45.0"
                     /created_by="primer3"
                     /modified_by="nicolegaudelli"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-B23-FLAG-ppBE4
                     (rAPOBEC1), pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq12; NMG-B25"
     primer_bind     2518..2537
                     /Sequence="GGCACCGAGGAACTGCTCGT"
                     /Hairpin_Tm="41.6"
                     /Tm="64.9"
                     /Self_Dimer_Tm="17.6"
                     /%GC="65.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="A03-Seq3000; A03-Seq3000"
     Primer          complement(2709..2726)
                     /created_by="yiyu"
                     /Transferred_From="pYY-B16-ScCas9 D10A R211K, N404K-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="106 G7 ApaI RP"
     misc_feature    complement(2907..2919)
                     /Recognition_pattern="(7/12)GAAGNNNNNNTAC(12/7)"
                     /Transferred_From="pYY-B16-ScCas9 D10A R211K, N404K-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="BarI"
                     /note="Geneious type: restriction site"
     primer_bind     2975..2994
                     /Sequence="CCGCCTTCCTGAGCGGCGAG"
                     /Hairpin_Tm="62.5"
                     /Tm="68.0"
                     /Self_Dimer_Tm="24.3"
                     /%GC="75.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B26"
     primer_bind     2988..3007
                     /Sequence="CGGCGAGCAGAAAAAGGCCA"
                     /Hairpin_Tm="40.5"
                     /Tm="64.0"
                     /Self_Dimer_Tm="5.0"
                     /%GC="60.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B4-ScCas9 D10A-NLS, pYY-B4-ScCas9
                     D10A-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="A01-Seq3500; A01-Seq3500"
     primer_bind     2993..3011
                     /Sequence="AGCAGAAAAAGGCCATCGT"
                     /X-Transferred_From="LCv2_seq13"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LCv2_seq13"
                     /Transferred_Similarity="100.00%"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-B23-FLAG-ppBE4
                     (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq13"
     primer_bind     3018..3037
                     /Sequence="GCTGTTCAAGACCAACCGGA"
                     /Hairpin_Tm="35.6"
                     /Tm="60.5"
                     /Self_Dimer_Tm="9.5"
                     /%GC="55.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="A03-Seq3500"
     primer_bind     complement(3054..3104)
                     /Sequence="ATTTCCACGGAGTCGAAGCACTCGATTTTCTTGAAGTAGTCCTCTTT
                     CAGC"
                     /%GC="45.1"
                     /Transferred_From="876_fzCas9-resplice"
                     /Transferred_Similarity="100.00%"
                     /X-Transferred_From="876_fzCas9-resplice"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-B23-FLAG-ppBE4
                     (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="876_fzCas9-resplice"
     primer_bind     complement(3054..3084)
                     /Sequence="CTCGATTTTCTTGAAGTAGTCCTCTTTCAGC"
                     /%GC="41.9"
                     /X-Transferred_From="901_fzCas9_NpuC-rev"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="901_fzCas9_NpuC-rev"
                     /Transferred_Similarity="100.00%"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-B23-FLAG-ppBE4
                     (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="901_fzCas9_NpuC-rev"
     primer_bind     3085..3107
                     /Sequence="TGCTTCGACTCCGTGGAAATCTC"
                     /%GC="52.2"
                     /X-Transferred_From="841_FZ_Cas9-C11-fwd"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="841_FZ_Cas9-C11-fwd"
                     /Transferred_Similarity="100.00%"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-B23-FLAG-ppBE4
                     (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="841_FZ_Cas9-C11-fwd"
     Primer          complement(3237..3260)
                     /created_by="Yi Yu"
                     /Transferred_From="pYY-B4-ScCas9 D10A-NLS, pYY-B4-ScCas9
                     D10A-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="YYA-P5 hScCas9A RP"
     USER            3248..3260
                     /created_by="Yi Yu"
                     /Transferred_From="pYY-B4-ScCas9 D10A-NLS, pYY-B4-ScCas9
                     D10A-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="USER3-A01"
     Primer          3248..3270
                     /created_by="Yi Yu"
                     /modified_by="Yi Yu"
                     /Transferred_From="pYY-B4-ScCas9 D10A-NLS, pYY-B4-ScCas9
                     D10A-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="YYA-P6 hScCas9B FP"
     primer_bind     3487..3506
                     /Sequence="ATCCAGAAAGCCCAGGTGTC"
                     /Hairpin_Tm="None"
                     /Tm="59.7"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /created_by="primer3"
                     /Transferred_From="LCv2_seq14"
                     /Transferred_Similarity="100.00%"
                     /X-Transferred_From="LCv2_seq14"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-B23-FLAG-ppBE4
                     (rAPOBEC1), pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq14; NMG-B159"
     primer_bind     3518..3537
                     /Sequence="ATAGCCTGCACGAGCACATT"
                     /Hairpin_Tm="48.1"
                     /Tm="60.1"
                     /Self_Dimer_Tm="2.5"
                     /%GC="50.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="A03-Seq4000"
     misc_feature    3840..3850
                     /Recognition_pattern="CCANNNN^NTGG"
                     /annotation_group="CCANNNNNTGG"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS, pYY-B2-SpCas9
                     D10A-NLS,pYY-B2-SpCas9 D10A-NLS , pYY-B3-SpCas9 D10A
                     H840A-NLS,pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B6-SpCas9-UGI-UGI-NLS,pYY-B6-SpCas9-UGI-UGI-NLS ,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS, pYY-B7-SpCas9
                     D10A-UGI-UGI-NLS ,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS,
                     pYY-B8-SpCas9 D10AH840-UGI-UGI-NLS , pYY-BGX13-hA3A-SpCas9
                     D10A-UGl,pYY-BGX26-mAID-SpCas9 D10A-UGl,
                     pYY-BGX27-pmCDA1-SpCas9D10A-UGl, pYY-BGX29-pmCDA5-SpCas9
                     D10A-UGI,pYY-BGX34-rAPOBEC1 W90Y
                     R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX2-TadA-TadA7.10-SpCas9
                     D10A,pYY-BX3-TadA7.10-SpCas9 D10A, pYY-BX4-A3A-SpCas9
                     D10A-UGl, pYY-CMn chimeric APOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="PflMI"
                     /note="Geneious type: restriction site"
     misc_feature    3840..3850
                     /Recognition_pattern="CCANNNN^NTGG"
                     /Transferred_From="pYY-B16-ScCas9 D10A R211K, N404K-NLS,
                     pYY-B21 (flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4 (rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1) ,
                     pYY-B23-FLAG-ppBE4 (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="PflMI"
                     /note="Geneious type: restriction site"
     primer_bind     3963..3982
                     /Sequence="GAGCGACAACGTGCCCTCCG"
                     /Hairpin_Tm="42.0"
                     /Tm="66.1"
                     /Self_Dimer_Tm="None"
                     /%GC="70.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B27"
     primer_bind     3980..3999
                     /Sequence="CCGAAGAGGTCGTGAAGAAG"
                     /Transferred_From="LCv2_seq15"
                     /Transferred_Similarity="100.00%"
                     /X-Transferred_From="LCv2_seq15"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-B23-FLAG-ppBE4
                     (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq15"
     primer_bind     4018..4037
                     /Sequence="CAGCTGCTGAACGCCAAGCT"
                     /Hairpin_Tm="45.7"
                     /Tm="64.0"
                     /Self_Dimer_Tm="24.9"
                     /%GC="60.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="A03-Seq4500; A03-Seq4500"
     misc_feature    4182..4194
                     /Recognition_pattern="(7/12)GAACNNNNNNTAC(12/7)"
                     /Transferred_From="pYY-B16-ScCas9 D10A R211K, N404K-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="PsrI"
                     /note="Geneious type: restriction site"
     primer_bind     4395..4414
                     /Sequence="CGGCGACTACAAGGTGTACG"
                     /Hairpin_Tm="None"
                     /Tm="60.8"
                     /Self_Dimer_Tm="None"
                     /%GC="60.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B28"
     primer_bind     4491..4510
                     /Sequence="CATGAACTTTTTCAAGACCG"
                     /Hairpin_Tm="45.0"
                     /Tm="53.1"
                     /Self_Dimer_Tm="None"
                     /%GC="40.0"
                     /modified_by="Yi Yu"
                     /Transferred_From="pYY-B4-ScCas9 D10A-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="A01-Seq5000"
     primer_bind     4510..4534
                     /Sequence="GAGATTACCCTGGCCAACGGCGAGA"
                     /Hairpin_Tm="53.7"
                     /Tm="68.3"
                     /Self_Dimer_Tm="16.6"
                     /%GC="60.0"
                     /created_by="primer3"
                     /modified_by="nicolegaudelli"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B274"
     primer_bind     4518..4537
                     /Sequence="CCTGGCCAACGGCGAGATCC"
                     /Hairpin_Tm="53.7"
                     /Tm="65.6"
                     /Self_Dimer_Tm="16.6"
                     /%GC="70.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="A03-Seq5000; A03-Seq5000"
     Primer          complement(4637..>4662)
                     /created_by="Yi Yu"
                     /modified_by="Yi Yu"
                     /Transferred_From="pYY-B16-ScCas9 D10A R211K, N404K-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="YY-AP12 hSpCas9 minus PI RP"
     misc_feature    complement(4685..4709)
                     /Sequence="TTCCTCTTGGGCAGGATAGACTCTT"
                     /Hairpin_Tm="39.9"
                     /Tm="62.8"
                     /Self_Dimer_Tm="None"
                     /%GC="48.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B275"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(4744..4767)
                     /Sequence="GAAGCCGCCGTACTTCTTAGGGTC"
                     /Hairpin_Tm="39.4"
                     /Tm="65.0"
                     /Self_Dimer_Tm="9.2"
                     /%GC="58.3"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-1606"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     4771..4792
                     /Sequence="AGCCCCACCGTGGCCTATTCTG"
                     /Hairpin_Tm="50.8"
                     /Tm="66.6"
                     /Self_Dimer_Tm="12.5"
                     /%GC="63.6"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-1605"
     misc_feature    4860..4869
                     /Recognition_pattern="GATNN^NNATC"
                     /Transferred_From="pYY-B1-SpCas9-NLS, pYY-B2-SpCas9
                     D10A-NLS, pYY-B2-SpCas9 D10A-NLS , pYY-B3-SpCas9 D10A
                     H840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS ,
                     pYY-B7-SpCas9 D10A-UGI-UGI-NLS, pYY-B7-SpCas9
                     D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS,
                     pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS ,
                     pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl"
                     /Transferred_Similarity="100.00%"
                     /label="BsaBI, Bse8I, BseJI"
                     /note="Geneious type: restriction site"
     primer_bind     4942..4961
                     /Sequence="GACCTGATCATCAAGCTGCC"
                     /Hairpin_Tm="None"
                     /Tm="58.7"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B29"
     misc_feature    complement(4995..5012)
                     /Sequence="GAGGCCAGCATTCTCTTC"
                     /Hairpin_Tm="None"
                     /Tm="55.1"
                     /Self_Dimer_Tm="5.0"
                     /%GC="55.6"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-1608"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     5018..5037
                     /created_by="Yi Yu; primer3"
                     /Sequence="GCGAACTGCAGAAGGGAAAC"
                     /Hairpin_Tm="45.5"
                     /Tm="59.8"
                     /Self_Dimer_Tm="23.4"
                     /%GC="55.0"
                     /modified_by="Yi Yu"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="A03-Seq5500; A03-Seq5500"
     primer_bind     5020..5044
                     /Sequence="GAACTGCAGAAGGGAAACGAACTGG"
                     /Hairpin_Tm="None"
                     /Tm="64.2"
                     /Self_Dimer_Tm="23.4"
                     /%GC="52.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-1607"
     primer_bind     5120..5146
                     /Sequence="ATGAGCAGAAACAGCTGTTTGTGGAAC"
                     /Hairpin_Tm="50.2"
                     /Tm="64.5"
                     /Self_Dimer_Tm="44.5"
                     /%GC="44.4"
                     /created_by="primer3"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B277"
     misc_feature    complement(5198..5222)
                     /Sequence="TTAGCGTCGGCCAGGATCACTCTCT"
                     /Hairpin_Tm="41.2"
                     /Tm="67.1"
                     /Self_Dimer_Tm="2.2"
                     /%GC="56.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B278"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(5346..5367)
                     /Sequence="CTTCCGGTCGATGGTGGTGTCA"
                     /Hairpin_Tm="None"
                     /Tm="64.5"
                     /Self_Dimer_Tm="None"
                     /%GC="59.1"
                     /created_by="primer3"
                     /modified_by="ngaudelli"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-1610"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    5377..5397
                     /Sequence="AGCACCAAAGAGGTGCTGGAC"
                     /Hairpin_Tm="77.5"
                     /Tm="62.9"
                     /Self_Dimer_Tm="33.6"
                     /%GC="57.1"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-1609 (VRER)"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     5436..5455
                     /Sequence="GACACGGATCGACCTGTCTC"
                     /Hairpin_Tm="40.4"
                     /Tm="59.9"
                     /Self_Dimer_Tm="18.1"
                     /%GC="60.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B30"
     misc_feature    complement(5447..5490)
                     /Sequence="AGATCCTCCGCTTCCACCAGAGTCACCTCCCAGCTGAGACAGGT"
                     /created_by="nicolegaudelli"
                     /Transferred_From="pYY-B23-FLAG-ppBE4 (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B291"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(5451..5488)
                     /created_by="yiyu"
                     /Sequence="ATCCTCCGCTTCCACCAGAGTCACCTCCCAGCTGAGAC"
                     /modified_by="yiyu"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="11 B6 RP"
     unsure          <5470..>6027
                     /created_by="ngaudelli"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS , pYY-B22-FLAG-BE4
                     (rAPOBEC1),pYY-B22-FLAG-BE4 (rAPOBEC1) ,
                     pYY-B23-FLAG-ppBE4(rAPOBEC1),
                     pYY-B6-SpCas9-UGI-UGI-NLS,pYY-B6-SpCas9-UGI-UGI-NLS ,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS, pYY-B7-SpCas9
                     D10A-UGI-UGI-NLS ,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS,
                     pYY-B8-SpCas9 D10AH840-UGI-UGI-NLS ,
                     pYY-BGX27-pmCDA1-SpCas9 D10A-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX4-A3A-SpCas9 D10A-UGl
                     , pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="gene block; gene block"
     CDS             5470..6027
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS , pYY-B22-FLAG-BE4
                     (rAPOBEC1),pYY-B22-FLAG-BE4 (rAPOBEC1) ,
                     pYY-B23-FLAG-ppBE4(rAPOBEC1),
                     pYY-B6-SpCas9-UGI-UGI-NLS,pYY-B6-SpCas9-UGI-UGI-NLS ,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS, pYY-B7-SpCas9
                     D10A-UGI-UGI-NLS ,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS,
                     pYY-B8-SpCas9 D10AH840-UGI-UGI-NLS ,
                     pYY-BGX27-pmCDA1-SpCas9 D10A-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX4-A3A-SpCas9 D10A-UGl
                     , pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="ORF1"
     primer_bind     5470..5488
                     /Sequence="TCTGGTGGAAGCGGAGGAT"
                     /Hairpin_Tm="46.3"
                     /Tm="60.3"
                     /Self_Dimer_Tm="None"
                     /%GC="57.9"
                     /modified_by="yiyu"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-B18-SaCas9
                     D10A-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="10 pCMV BB UGI FP; 10 pCMV BB UGI FP"
     misc_feature    complement(5470..5490)
                     /Sequence="AGATCCTCCGCTTCCACCAGA"
                     /Hairpin_Tm="None"
                     /Tm="62.7"
                     /Self_Dimer_Tm="None"
                     /%GC="57.1"
                     /modified_by="nicolegaudelli"
                     /Transferred_From="pYY-BX2-TadA-TadA7.10-SpCas9 D10A"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B363"
                     /note="Geneious type: primer_bind_reverse"
     unsure          <5470..>5499
                     /created_by="ngaudelli"
                     /Transferred_From="pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl ,
                     pYY-BX2-TadA-TadA7.10-SpCas9 D10A, pYY-BX3-TadA7.10-SpCas9
                     D10A"
                     /Transferred_Similarity="100.00%"
                     /label="gene block"
     USER            5478..5490
                     /created_by="ngaudelli"
                     /Transferred_From="pYY-B22-FLAG-BE4 (rAPOBEC1),
                     pYY-B22-FLAG-BE4 (rAPOBEC1) , pYY-B23-FLAG-ppBE4
                     (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
     primer_bind     5478..5499
                     /Sequence="AAGCGGAGGATCTGGCGGCAGC"
                     /Hairpin_Tm="43.4"
                     /Tm="69.6"
                     /Self_Dimer_Tm="32.4"
                     /%GC="68.2"
                     /created_by="primer3"
                     /Transferred_From="pYY-B23-FLAG-ppBE4 (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B225"
     USER            5478..5490
                     /created_by="ngaudelli"
                     /Transferred_From="pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl ,
                     pYY-BX2-TadA-TadA7.10-SpCas9 D10A, pYY-BX3-TadA7.10-SpCas9
                     D10A"
                     /Transferred_Similarity="100.00%"
     CDS             5479..>5490
                     /created_by="ngaudelli"
                     /Transferred_From="pYY-BGn-deaminase-NLS Copy"
                     /Transferred_Similarity="100.00%"
                     /label="linker"
     unsure          <5500..>6027
                     /created_by="ngaudelli"
                     /Transferred_From="pYY-BGX13-hA3A-SpCas9 D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl, pYY-BGX29-pmCDA5-SpCas9
                     D10A-UGI, pYY-BGX34-rAPOBEC1 W90Y R126E-UGl"
                     /Transferred_Similarity="100.00%"
                     /label="gene block"
     CDS             <5500..6027
                     /Transferred_From="pYY-BGX13-hA3A-SpCas9 D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl, pYY-BGX29-pmCDA5-SpCas9
                     D10A-UGI, pYY-BGX34-rAPOBEC1 W90Y R126E-UGl"
                     /Transferred_Similarity="100.00%"
                     /label="ORF1"
     misc_feature    complement(5996..6026)
                     /Sequence="AGCATTTTGATTTTGTTTTCCCCATTGGAGT"
                     /Hairpin_Tm="46.2"
                     /Tm="64.9"
                     /Self_Dimer_Tm="3.6"
                     /%GC="35.5"
                     /created_by="primer3"
                     /Transferred_From="pYY-AS22 BE4 Y120A"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B144"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(6006..6047)
                     /Sequence="ACCTTGCGTTTTTTCTTTGGGAGCATTTTGATTTTGTTTTCC"
                     /created_by="ngaudelli"
                     /Transferred_From="pYY-B23-FLAG-ppBE4 (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B224"
                     /note="Geneious type: primer_bind_reverse"
     CDS             <6028..6118
                     /created_by="nicolegaudelli"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-BGX13-hA3A-SpCas9
                     D10A-UGl,pYY-BGX26-mAID-SpCas9 D10A-UGl,
                     pYY-BGX27-pmCDA1-SpCas9D10A-UGl, pYY-BGX29-pmCDA5-SpCas9
                     D10A-UGI,pYY-BGX34-rAPOBEC1 W90Y
                     R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX4-A3A-SpCas9 D10A-UGl
                     , pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="NLS tag"
     primer_bind     6028..6050
                     /Mismatches=0
                     /%GC="43.5"
                     /Hairpin_Tm="46.4"
                     /Self_Dimer_Tm="None"
                     /Tm="60.7"
                     /Sequence="CCAAAGAAAAAACGCAAGGTGGA"
                     /created_by="primer3"
                     /Transferred_From="pYY-B12-pmCDA-NLS, pYY-B23-FLAG-ppBE4
                     (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="2 pCMV BB NLS FP"
     CDS             6028..6117
                     /created_by="yiyu"
                     /modified_by="yiyu"
                     /Transferred_From="pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS, pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS, pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS, pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS, pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1 (1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B16-ScCas9 D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS, pYY-B18-SaCas9 D10A-UGI-UGI-NLS, pYY-B18-SaCas9
                     D10A-UGI-UGI-NLS , pYY-B19-Flag-TadA-TadA7.10*-NLS,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS , pYY-B2-SpCas9 D10A-NLS,
                     pYY-B2-SpCas9 D10A-NLS , pYY-B20-flag-rAPOBEC1-NLS,
                     pYY-B20-flag-rAPOBEC1-NLS , pYY-B22-FLAG-BE4 (rAPOBEC1),
                     pYY-B22-FLAG-BE4 (rAPOBEC1) , pYY-B23-FLAG-ppBE4
                     (rAPOBEC1), pYY-B3-SpCas9 D10A H840A-NLS, pYY-B3-SpCas9
                     D10A H840A-NLS , pYY-B4-ScCas9 D10A-NLS, pYY-B4-ScCas9
                     D10A-NLS , pYY-B5-Sp-ScCas9 D10A-NLS, pYY-B5-Sp-ScCas9
                     D10A-NLS , pYY-B6-SpCas9-UGI-UGI-NLS,
                     pYY-B6-SpCas9-UGI-UGI-NLS , pYY-B7-SpCas9
                     D10A-UGI-UGI-NLS, pYY-B7-SpCas9 D10A-UGI-UGI-NLS ,
                     pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS, pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS , pYY-B9 mutation-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS, pYY-B9-rAPOBEC1-NLS ,
                     pYY-BG34-rAPOBEC1 W90Y R126E-NLS, pYY-BG35 rAPOBEC1 W90Y
                     R126E R132E, pYY-BGn-deaminase-NLS Copy"
                     /Transferred_Similarity="100.00%"
                     /label="BPNLS"
     primer_bind     <6031..6051
                     /created_by="nicolegaudelli"
                     /Sequence="ACAAGCCCAAGCTGGACTTCCCAAAGAAAAAACGCAAGGTGGAG"
                     /modified_by="nicolegaudelli"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B354"
     USER            6035..6047
                     /modified_by="ngaudelli"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-BGX13-hA3A-SpCas9
                     D10A-UGl,pYY-BGX26-mAID-SpCas9 D10A-UGl,
                     pYY-BGX27-pmCDA1-SpCas9D10A-UGl, pYY-BGX29-pmCDA5-SpCas9
                     D10A-UGI,pYY-BGX34-rAPOBEC1 W90Y
                     R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX2-TadA-TadA7.10-SpCas9
                     D10A,pYY-BX3-TadA7.10-SpCas9 D10A, pYY-BX4-A3A-SpCas9
                     D10A-UGl, pYY-CMn chimeric APOBECs"
                     /Transferred_Similarity="100.00%"
     primer_bind     6035..6081
                     /Sequence="AAAAACGCAAGGTGGAGGGAGCTGATAAGCGCACCGCCGATGGTTCC
                     "
                     /created_by="ngaudelli"
                     /created_by="ngaudelli"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B223"
     USER            6035..6047
                     /modified_by="Yi Yu"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="USER2-A03"
     Primer          <6035..6055
                     /created_by="Yi Yu"
                     /modified_by="Yi Yu"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="YYA-P9 A03 BB FP"
     source          <6049..>6060
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /modified_by="hrees"
                     /modified_by="hrees"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS,pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS,pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS,pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS,pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1(1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B16-ScCas9D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS,pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS ,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS,pYY-B19-Flag-TadA-TadA7.10
                     *-NLS , pYY-B2-SpCas9 D10A-NLS,pYY-B2-SpCas9 D10A-NLS ,
                     pYY-B20-flag-rAPOBEC1-NLS,pYY-B20-flag-rAPOBEC1-NLS ,
                     pYY-B21(flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4(rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1)
                     ,pYY-B23-FLAG-ppBE4 (rAPOBEC1), pYY-B3-SpCas9
                     D10AH840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B4-ScCas9D10A-NLS, pYY-B4-ScCas9 D10A-NLS ,
                     pYY-B5-Sp-ScCas9D10A-NLS, pYY-B5-Sp-ScCas9 D10A-NLS
                     ,pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS
                     ,pYY-B7-SpCas9 D10A-UGI-UGI-NLS,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS ,
                     pYY-B9mutation-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS,pYY-B9-rAPOBEC1-NLS ,
                     pYY-BG34-rAPOBEC1 W90Y R126E-NLS,pYY-BG35 rAPOBEC1 W90Y
                     R126E R132E, pYY-BGX13-hA3A-SpCas9D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl,pYY-BGX27-pmCDA1-SpCas9
                     D10A-UGl, pYY-BGX29-pmCDA5-SpCas9D10A-UGI,
                     pYY-BGX34-rAPOBEC1 W90Y R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BGn-deaminase-NLSCopy, pYY-BX1-pmCDA-SpCas9
                     D10A-UGl,pYY-BX2-TadA-TadA7.10-SpCas9 D10A,
                     pYY-BX3-TadA7.10-SpCas9D10A, pYY-BX4-A3A-SpCas9 D10A-UGl ,
                     pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="To be added in"
     Primer          6061..>6093
                     /created_by="hrees"
                     /created_by="hrees"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A),pYY-BGX13-hA3A-SpCas9
                     D10A-UGl, pYY-BGX26-mAID-SpCas9D10A-UGl,
                     pYY-BGX27-pmCDA1-SpCas9 D10A-UGl,pYY-BGX29-pmCDA5-SpCas9
                     D10A-UGI, pYY-BGX34-rAPOBEC1 W90YR126E-UGl,
                     pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX2-TadA-TadA7.10-SpCas9
                     D10A,pYY-BX3-TadA7.10-SpCas9 D10A, pYY-BX4-A3A-SpCas9
                     D10A-UGl, pYY-CMn chimeric APOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="BPNLS"
     source          <6061..>6093
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /modified_by="hrees"
                     /modified_by="hrees"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS,pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS,pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS,pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS,pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1(1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B16-ScCas9D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS,pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS ,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS,pYY-B19-Flag-TadA-TadA7.10
                     *-NLS , pYY-B2-SpCas9 D10A-NLS,pYY-B2-SpCas9 D10A-NLS ,
                     pYY-B20-flag-rAPOBEC1-NLS,pYY-B20-flag-rAPOBEC1-NLS ,
                     pYY-B21(flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4(rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1)
                     ,pYY-B23-FLAG-ppBE4 (rAPOBEC1), pYY-B3-SpCas9
                     D10AH840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B4-ScCas9D10A-NLS, pYY-B4-ScCas9 D10A-NLS ,
                     pYY-B5-Sp-ScCas9D10A-NLS, pYY-B5-Sp-ScCas9 D10A-NLS
                     ,pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS
                     ,pYY-B7-SpCas9 D10A-UGI-UGI-NLS,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS ,
                     pYY-B9mutation-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS,pYY-B9-rAPOBEC1-NLS ,
                     pYY-BG34-rAPOBEC1 W90Y R126E-NLS,pYY-BG35 rAPOBEC1 W90Y
                     R126E R132E, pYY-BGX13-hA3A-SpCas9D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl,pYY-BGX27-pmCDA1-SpCas9
                     D10A-UGl, pYY-BGX29-pmCDA5-SpCas9D10A-UGI,
                     pYY-BGX34-rAPOBEC1 W90Y R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BGn-deaminase-NLSCopy, pYY-BX1-pmCDA-SpCas9
                     D10A-UGl,pYY-BX2-TadA-TadA7.10-SpCas9 D10A,
                     pYY-BX3-TadA7.10-SpCas9D10A, pYY-BX4-A3A-SpCas9 D10A-UGl ,
                     pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="To be added in"
     Primer          6067..>6115
                     /created_by="hrees"
                     /modified_by="kzhao"
                     /created_by="hrees"
                     /modified_by="kzhao"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A),pYY-BGX13-hA3A-SpCas9
                     D10A-UGl, pYY-BGX26-mAID-SpCas9D10A-UGl,
                     pYY-BGX27-pmCDA1-SpCas9 D10A-UGl,pYY-BGX29-pmCDA5-SpCas9
                     D10A-UGI, pYY-BGX34-rAPOBEC1 W90YR126E-UGl,
                     pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX2-TadA-TadA7.10-SpCas9
                     D10A,pYY-BX3-TadA7.10-SpCas9 D10A, pYY-BX4-A3A-SpCas9
                     D10A-UGl, pYY-CMn chimeric APOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="HR898"
     CDS             6094..6117
                     /created_by="User"
                     /modified_by="User"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A),pYY-BGX13-hA3A-SpCas9
                     D10A-UGl, pYY-BGX26-mAID-SpCas9D10A-UGl,
                     pYY-BGX27-pmCDA1-SpCas9 D10A-UGl,pYY-BGX29-pmCDA5-SpCas9
                     D10A-UGI, pYY-BGX34-rAPOBEC1 W90YR126E-UGl,
                     pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX2-TadA-TadA7.10-SpCas9
                     D10A,pYY-BX3-TadA7.10-SpCas9 D10A, pYY-BX4-A3A-SpCas9
                     D10A-UGl, pYY-CMn chimeric APOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="SV40 NLS"
     USER            <6118..6128
                     /created_by="nicolegaudelli"
                     /Transferred_From="pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS, pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B16-ScCas9 D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS, pYY-B19-Flag-TadA-TadA7.10*-NLS,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS , pYY-B2-SpCas9 D10A-NLS,
                     pYY-B2-SpCas9 D10A-NLS , pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A), pYY-B3-SpCas9 D10A
                     H840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS , pYY-B4-ScCas9
                     D10A-NLS, pYY-B4-ScCas9 D10A-NLS , pYY-B5-Sp-ScCas9
                     D10A-NLS, pYY-B5-Sp-ScCas9 D10A-NLS"
                     /Transferred_Similarity="100.00%"
     misc_feature    complement(6163..6180)
                     /Sequence="TAGAAGGCACAGTCGAGG"
                     /Hairpin_Tm="None"
                     /Tm="55.6"
                     /Self_Dimer_Tm="None"
                     /%GC="55.6"
                     /created_by="primer3"
                     /modified_by="User"
                     /Transferred_From="pYY-AS22 BE4 Y120A"
                     /Transferred_Similarity="100.00%"
                     /label="bgh reverse primer"
                     /note="Geneious type: primer_bind_reverse"
     terminator      complement(6166..6393)
                     /created_by="User"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS,pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS,pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS,pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS,pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1(1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B16-ScCas9D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS,pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS ,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS,pYY-B19-Flag-TadA-TadA7.10
                     *-NLS , pYY-B2-SpCas9 D10A-NLS,pYY-B2-SpCas9 D10A-NLS ,
                     pYY-B20-flag-rAPOBEC1-NLS,pYY-B20-flag-rAPOBEC1-NLS ,
                     pYY-B21(flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4(rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1)
                     ,pYY-B23-FLAG-ppBE4 (rAPOBEC1), pYY-B3-SpCas9
                     D10AH840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B4-ScCas9D10A-NLS, pYY-B4-ScCas9 D10A-NLS ,
                     pYY-B5-Sp-ScCas9D10A-NLS, pYY-B5-Sp-ScCas9 D10A-NLS
                     ,pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS
                     ,pYY-B7-SpCas9 D10A-UGI-UGI-NLS,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS ,
                     pYY-B9mutation-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS,pYY-B9-rAPOBEC1-NLS ,
                     pYY-BG34-rAPOBEC1 W90Y R126E-NLS,pYY-BG35 rAPOBEC1 W90Y
                     R126E R132E, pYY-BGX13-hA3A-SpCas9D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl,pYY-BGX27-pmCDA1-SpCas9
                     D10A-UGl, pYY-BGX29-pmCDA5-SpCas9D10A-UGI,
                     pYY-BGX34-rAPOBEC1 W90Y R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BGn-deaminase-NLSCopy, pYY-BX1-pmCDA-SpCas9
                     D10A-UGl,pYY-BX2-TadA-TadA7.10-SpCas9 D10A,
                     pYY-BX3-TadA7.10-SpCas9D10A, pYY-BX4-A3A-SpCas9 D10A-UGl ,
                     pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="bgH terminator"
     misc_feature    complement(6460..6478)
                     /Sequence="GGAAACAGCTATGACCATG"
                     /Hairpin_Tm="None"
                     /Tm="53.4"
                     /Self_Dimer_Tm="None"
                     /%GC="47.4"
                     /created_by="primer3"
                     /Transferred_From="pYY-AS22 BE4 Y120A"
                     /Transferred_Similarity="100.00%"
                     /label="M13 reverse"
                     /note="Geneious type: primer_bind_reverse"
     promoter        complement(6513..6542)
                     /created_by="User"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS,pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS,pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS,pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS,pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1(1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B16-ScCas9D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS,pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS ,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS,pYY-B19-Flag-TadA-TadA7.10
                     *-NLS , pYY-B2-SpCas9 D10A-NLS,pYY-B2-SpCas9 D10A-NLS ,
                     pYY-B20-flag-rAPOBEC1-NLS,pYY-B20-flag-rAPOBEC1-NLS ,
                     pYY-B21(flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4(rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1)
                     ,pYY-B23-FLAG-ppBE4 (rAPOBEC1), pYY-B3-SpCas9
                     D10AH840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B4-ScCas9D10A-NLS, pYY-B4-ScCas9 D10A-NLS ,
                     pYY-B5-Sp-ScCas9D10A-NLS, pYY-B5-Sp-ScCas9 D10A-NLS
                     ,pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS
                     ,pYY-B7-SpCas9 D10A-UGI-UGI-NLS,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS ,
                     pYY-B9mutation-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS,pYY-B9-rAPOBEC1-NLS ,
                     pYY-BG34-rAPOBEC1 W90Y R126E-NLS,pYY-BG35 rAPOBEC1 W90Y
                     R126E R132E, pYY-BGX13-hA3A-SpCas9D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl,pYY-BGX27-pmCDA1-SpCas9
                     D10A-UGl, pYY-BGX29-pmCDA5-SpCas9D10A-UGI,
                     pYY-BGX34-rAPOBEC1 W90Y R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BGn-deaminase-NLSCopy, pYY-BX1-pmCDA-SpCas9
                     D10A-UGl,pYY-BX2-TadA-TadA7.10-SpCas9 D10A,
                     pYY-BX3-TadA7.10-SpCas9D10A, pYY-BX4-A3A-SpCas9 D10A-UGl ,
                     pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="lac promoter"
     primer_bind     6539..6569
                     /Sequence="TAAAGCCTAGGGTGCCTAATGAGTGAGCTAA"
                     /Hairpin_Tm="35.5"
                     /Tm="66.7"
                     /Self_Dimer_Tm="None"
                     /%GC="45.2"
                     /created_by="primer3"
                     /Transferred_From="pYY-AS22 BE4 Y120A"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-850"
     misc_feature    complement(6658..6668)
                     /Recognition_pattern="(9/12)ACNNNNNCTCC(10/7)"
                     /Transferred_From="pYY-B11-TadA7.10*-NLS,
                     pYY-B11-TadA7.10*-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="BsaXI"
                     /note="Geneious type: restriction site"
     primer_bind     6663..6682
                     /Mismatches=0
                     /%GC="65.0"
                     /Hairpin_Tm="47.9"
                     /Self_Dimer_Tm="None"
                     /Tm="65.2"
                     /Sequence="GGCGGTTTGCGTATTGGGCG"
                     /created_by="primer3"
                     /Transferred_From="pYY-BGn-deaminase-NLS Copy"
                     /Transferred_Similarity="100.00%"
                     /label="Miseq FP"
     rep_origin      6851..7470
                     /created_by="User"
                     /modified_by="User"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS,pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS,pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS,pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS,pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1(1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B16-ScCas9D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS,pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS ,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS,pYY-B19-Flag-TadA-TadA7.10
                     *-NLS , pYY-B2-SpCas9 D10A-NLS,pYY-B2-SpCas9 D10A-NLS ,
                     pYY-B20-flag-rAPOBEC1-NLS,pYY-B20-flag-rAPOBEC1-NLS ,
                     pYY-B21(flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4(rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1)
                     ,pYY-B23-FLAG-ppBE4 (rAPOBEC1), pYY-B3-SpCas9
                     D10AH840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B4-ScCas9D10A-NLS, pYY-B4-ScCas9 D10A-NLS ,
                     pYY-B5-Sp-ScCas9D10A-NLS, pYY-B5-Sp-ScCas9 D10A-NLS
                     ,pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS
                     ,pYY-B7-SpCas9 D10A-UGI-UGI-NLS,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS ,
                     pYY-B9mutation-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS,pYY-B9-rAPOBEC1-NLS ,
                     pYY-BG34-rAPOBEC1 W90Y R126E-NLS,pYY-BG35 rAPOBEC1 W90Y
                     R126E R132E, pYY-BGX13-hA3A-SpCas9D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl,pYY-BGX27-pmCDA1-SpCas9
                     D10A-UGl, pYY-BGX29-pmCDA5-SpCas9D10A-UGI,
                     pYY-BGX34-rAPOBEC1 W90Y R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BGn-deaminase-NLSCopy, pYY-BX1-pmCDA-SpCas9
                     D10A-UGl,pYY-BX2-TadA-TadA7.10-SpCas9 D10A,
                     pYY-BX3-TadA7.10-SpCas9D10A, pYY-BX4-A3A-SpCas9 D10A-UGl ,
                     pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="pBR322"
     misc_feature    6907..6918
                     /Recognition_pattern="GACNNNN^NNGTC"
                     /Transferred_From="pYY-B11-TadA7.10*-NLS,
                     pYY-B11-TadA7.10*-NLS , pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl"
                     /Transferred_Similarity="100.00%"
                     /label="DrdI, AasI, DseDI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(6908..6928)
                     /Mismatches=0
                     /%GC="61.9"
                     /Hairpin_Tm="37.7"
                     /Self_Dimer_Tm="None"
                     /Tm="64.9"
                     /Sequence="CGCCACCTCTGACTTGAGCGT"
                     /created_by="primer3"
                     /Transferred_From="pYY-BGn-deaminase-NLS Copy"
                     /Transferred_Similarity="100.00%"
                     /label="Miseq RP"
     primer_bind     7074..7101
                     /Sequence="AGGTATCTCAGTTCGGTGTAGGTCGTTC"
                     /Hairpin_Tm="None"
                     /Tm="65.5"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-AS22 BE4 Y120A"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-851"
     primer_bind     7617..7645
                     /Sequence="TCTGACAGTTACCAATGCTTAATCAGTGA"
                     /Hairpin_Tm="34.3"
                     /Tm="62.7"
                     /Self_Dimer_Tm="8.2"
                     /%GC="37.9"
                     /created_by="primer3"
                     /Transferred_From="pYY-AS22 BE4 Y120A"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-853"
     CDS             complement(7625..8485)
                     /created_by="User"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS,pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS,pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS,pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS,pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1(1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B16-ScCas9D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS,pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS ,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS,pYY-B19-Flag-TadA-TadA7.10
                     *-NLS , pYY-B2-SpCas9 D10A-NLS,pYY-B2-SpCas9 D10A-NLS ,
                     pYY-B20-flag-rAPOBEC1-NLS,pYY-B20-flag-rAPOBEC1-NLS ,
                     pYY-B21(flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4(rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1)
                     ,pYY-B23-FLAG-ppBE4 (rAPOBEC1), pYY-B3-SpCas9
                     D10AH840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B4-ScCas9D10A-NLS, pYY-B4-ScCas9 D10A-NLS ,
                     pYY-B5-Sp-ScCas9D10A-NLS, pYY-B5-Sp-ScCas9 D10A-NLS
                     ,pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS
                     ,pYY-B7-SpCas9 D10A-UGI-UGI-NLS,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS ,
                     pYY-B9mutation-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS,pYY-B9-rAPOBEC1-NLS ,
                     pYY-BG34-rAPOBEC1 W90Y R126E-NLS,pYY-BG35 rAPOBEC1 W90Y
                     R126E R132E, pYY-BGX13-hA3A-SpCas9D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl,pYY-BGX27-pmCDA1-SpCas9
                     D10A-UGl, pYY-BGX29-pmCDA5-SpCas9D10A-UGI,
                     pYY-BGX34-rAPOBEC1 W90Y R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BGn-deaminase-NLSCopy, pYY-BX1-pmCDA-SpCas9
                     D10A-UGl,pYY-BX2-TadA-TadA7.10-SpCas9 D10A,
                     pYY-BX3-TadA7.10-SpCas9D10A, pYY-BX4-A3A-SpCas9 D10A-UGl ,
                     pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="beta-lactamase"
     misc_feature    7693..7703
                     /Recognition_pattern="GACNNN^NNGTC"
                     /Transferred_From="pYY-B11-TadA7.10*-NLS,
                     pYY-B11-TadA7.10*-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="Eam1105I, AhdI, BmeRI, DriI"
                     /note="Geneious type: restriction site"
     primer_bind     8149..8177
                     /Sequence="CCGTAAGATGCTTTTCTGTGACTGGTGAG"
                     /Hairpin_Tm="43.0"
                     /Tm="65.6"
                     /Self_Dimer_Tm="None"
                     /%GC="48.3"
                     /created_by="primer3"
                     /Transferred_From="pYY-AS22 BE4 Y120A"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-854"
     misc_feature    8293..8302
                     /Recognition_pattern="GAANN^NNTTC"
                     /Transferred_From="pYY-B11-TadA7.10*-NLS,
                     pYY-B11-TadA7.10*-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="XmnI, Asp700I, MroXI, PdmI"
                     /note="Geneious type: restriction site"
     promoter        complement(8527..8555)
                     /created_by="User"
                     /modified_by="User"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS,pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS,pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS,pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS,pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1(1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B16-ScCas9D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS,pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS ,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS,pYY-B19-Flag-TadA-TadA7.10
                     *-NLS , pYY-B2-SpCas9 D10A-NLS,pYY-B2-SpCas9 D10A-NLS ,
                     pYY-B20-flag-rAPOBEC1-NLS,pYY-B20-flag-rAPOBEC1-NLS ,
                     pYY-B21(flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4(rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1)
                     ,pYY-B23-FLAG-ppBE4 (rAPOBEC1), pYY-B3-SpCas9
                     D10AH840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B4-ScCas9D10A-NLS, pYY-B4-ScCas9 D10A-NLS ,
                     pYY-B5-Sp-ScCas9D10A-NLS, pYY-B5-Sp-ScCas9 D10A-NLS
                     ,pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS
                     ,pYY-B7-SpCas9 D10A-UGI-UGI-NLS,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS ,
                     pYY-B9mutation-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS,pYY-B9-rAPOBEC1-NLS ,
                     pYY-BG34-rAPOBEC1 W90Y R126E-NLS,pYY-BG35 rAPOBEC1 W90Y
                     R126E R132E, pYY-BGX13-hA3A-SpCas9D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl,pYY-BGX27-pmCDA1-SpCas9
                     D10A-UGl, pYY-BGX29-pmCDA5-SpCas9D10A-UGI,
                     pYY-BGX34-rAPOBEC1 W90Y R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BGn-deaminase-NLSCopy, pYY-BX1-pmCDA-SpCas9
                     D10A-UGl,pYY-BX2-TadA-TadA7.10-SpCas9 D10A,
                     pYY-BX3-TadA7.10-SpCas9D10A, pYY-BX4-A3A-SpCas9 D10A-UGl ,
                     pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="pAmpR"
     primer_bind     8606..8634
                     /Sequence="AGTGCCACCTGACGTCGACGGATCGGGAG"
                     /Hairpin_Tm="48.7"
                     /Tm="74.0"
                     /Self_Dimer_Tm="33.5"
                     /%GC="65.5"
                     /created_by="primer3"
                     /Transferred_From="pYY-AS22 BE4 Y120A"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-838"
ORIGIN      
        1 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
       61 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      121 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      181 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      241 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      301 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      361 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      421 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      481 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      541 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      601 tctatataag cagagctggt ttagtgaacc gtcagatccg ctagagatcc gcggccgcta
      661 atacgactca ctatagggag agccgccacc atgaagcccc agatcaggga ccaccgcccc
      721 aatcctatgg aggccatgta ccctcacatc ttctattttc acttcgagaa cctggagaag
      781 gcctacggcc ggaatgagac ctggctgtgc tttacagtgg agatcatcaa gcagtatctg
      841 ccagtgccct ggaagaaggg cgtgttccgg aaccaggtgg atccagagac ccactgccac
      901 gccgagaagt gttttctgtc ctggttctgt aacaatacac tgtctcccaa gaagaattac
      961 caggtgacct ggtatacaag ctggtcccct tgcccagagt gtgcaggaga ggtggcagag
     1021 tttctggcag agcacagcaa cgtgaagctg accatctaca cagcccggct gtactatctg
     1081 tgggacaccg attatcagga gggcctgaga tctctgagcg aggagggcgc ctccgtggag
     1141 atcatggact acgaggattt tcagtattgc tgggagaact tcgtgtacga cgatggcgag
     1201 ccttttaaga ggtggaaggg cctgaagtat aatttccagt ctctgacacg gagactgcgc
     1261 gagatcctgc agagcggagg atctagcggc ggctctagcg gatctgagac acctggcaca
     1321 agcgagtctg ccacacctga gagtagcggc ggatcttctg gtggctctga caagaagtac
     1381 agcatcggcc tggccatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac
     1441 aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag
     1501 aacctgatcg gagccctgct gttcgacagc ggcgaaacag ccgaggccac ccggctgaag
     1561 agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc
     1621 ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga agagtccttc
     1681 ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat cgtggacgag
     1741 gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact ggtggacagc
     1801 accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat caagttccgg
     1861 ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga caagctgttc
     1921 atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa cgccagcggc
     1981 gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagacggct ggaaaatctg
     2041 atcgcccagc tgcccggcga gaagaagaat ggcctgttcg gaaacctgat tgccctgagc
     2101 ctgggcctga cccccaactt caagagcaac ttcgacctgg ccgaggatgc caaactgcag
     2161 ctgagcaagg acacctacga cgacgacctg gacaacctgc tggcccagat cggcgaccag
     2221 tacgccgacc tgtttctggc cgccaagaac ctgtccgacg ccatcctgct gagcgacatc
     2281 ctgagagtga acaccgagat caccaaggcc cccctgagcg cctctatgat caagagatac
     2341 gacgagcacc accaggacct gaccctgctg aaagctctcg tgcggcagca gctgcctgag
     2401 aagtacaaag agattttctt cgaccagagc aagaacggct acgccggcta cattgacggc
     2461 ggagccagcc aggaagagtt ctacaagttc atcaagccca tcctggaaaa gatggacggc
     2521 accgaggaac tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca gcggaccttc
     2581 gacaacggca gcatccccca ccagatccac ctgggagagc tgcacgccat tctgcggcgg
     2641 caggaagatt tttacccatt cctgaaggac aaccgggaaa agatcgagaa gatcctgacc
     2701 ttccgcatcc cctactacgt gggccctctg gccaggggaa acagcagatt cgcctggatg
     2761 accagaaaga gcgaggaaac catcaccccc tggaacttcg aggaagtggt ggacaagggc
     2821 gcttccgccc agagcttcat cgagcggatg accaacttcg ataagaacct gcccaacgag
     2881 aaggtgctgc ccaagcacag cctgctgtac gagtacttca ccgtgtataa cgagctgacc
     2941 aaagtgaaat acgtgaccga gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa
     3001 aaggccatcg tggacctgct gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa
     3061 gaggactact tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg cgtggaagat
     3121 cggttcaacg cctccctggg cacataccac gatctgctga aaattatcaa ggacaaggac
     3181 ttcctggaca atgaggaaaa cgaggacatt ctggaagata tcgtgctgac cctgacactg
     3241 tttgaggaca gagagatgat cgaggaacgg ctgaaaacct atgcccacct gttcgacgac
     3301 aaagtgatga agcagctgaa gcggcggaga tacaccggct ggggcaggct gagccggaag
     3361 ctgatcaacg gcatccggga caagcagtcc ggcaagacaa tcctggattt cctgaagtcc
     3421 gacggcttcg ccaacagaaa cttcatgcag ctgatccacg acgacagcct gacctttaaa
     3481 gaggacatcc agaaagccca ggtgtccggc cagggcgata gcctgcacga gcacattgcc
     3541 aatctggccg gcagccccgc cattaagaag ggcatcctgc agacagtgaa ggtggtggac
     3601 gagctcgtga aagtgatggg ccggcacaag cccgagaaca tcgtgatcga aatggccaga
     3661 gagaaccaga ccacccagaa gggacagaag aacagccgcg agagaatgaa gcggatcgaa
     3721 gagggcatca aagagctggg cagccagatc ctgaaagaac accccgtgga aaacacccag
     3781 ctgcagaacg agaagctgta cctgtactac ctgcagaatg ggcgggatat gtacgtggac
     3841 caggaactgg acatcaaccg gctgtccgac tacgatgtgg accatatcgt gcctcagagc
     3901 tttctgaagg acgactccat cgacaacaag gtgctgacca gaagcgacaa gaaccggggc
     3961 aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta ctggcggcag
     4021 ctgctgaacg ccaagctgat tacccagaga aagttcgaca atctgaccaa ggccgagaga
     4081 ggcggcctga gcgaactgga taaggccggc ttcatcaaga gacagctggt ggaaacccgg
     4141 cagatcacaa agcacgtggc acagatcctg gactcccgga tgaacactaa gtacgacgag
     4201 aatgacaagc tgatccggga agtgaaagtg atcaccctga agtccaagct ggtgtccgat
     4261 ttccggaagg atttccagtt ttacaaagtg cgcgagatca acaactacca ccacgcccac
     4321 gacgcctacc tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc taagctggaa
     4381 agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc
     4441 gagcaggaaa tcggcaaggc taccgccaag tacttcttct acagcaacat catgaacttt
     4501 ttcaagaccg agattaccct ggccaacggc gagatccgga agcggcctct gatcgagaca
     4561 aacggcgaaa ccggggagat cgtgtgggat aagggccggg attttgccac cgtgcggaaa
     4621 gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc
     4681 agcaaagagt ctatcctgcc caagaggaac agcgataagc tgatcgccag aaagaaggac
     4741 tgggacccta agaagtacgg cggcttcgac agccccaccg tggcctattc tgtgctggtg
     4801 gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga gctgctgggg
     4861 atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct ggaagccaag
     4921 ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc cctgttcgag
     4981 ctggaaaacg gccggaagag aatgctggcc tctgccggcg aactgcagaa gggaaacgaa
     5041 ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta tgagaagctg
     5101 aagggctccc ccgaggataa tgagcagaaa cagctgtttg tggaacagca caagcactac
     5161 ctggacgaga tcatcgagca gatcagcgag ttctccaaga gagtgatcct ggccgacgct
     5221 aatctggaca aagtgctgtc cgcctacaac aagcaccggg ataagcccat cagagagcag
     5281 gccgagaata tcatccacct gtttaccctg accaatctgg gagcccctgc cgccttcaag
     5341 tactttgaca ccaccatcga ccggaagagg tacaccagca ccaaagaggt gctggacgcc
     5401 accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct gtctcagctg
     5461 ggaggtgact ctggtggaag cggaggatct ggcggcagca ccaatctgag cgacatcatc
     5521 gagaaagaga caggcaagca gctggtcatc caagagtcca tcctgatgct gcctgaagag
     5581 gtggaagaag tgatcggcaa caagcccgag tccgacatcc tggtgcacac cgcctacgat
     5641 gagagcaccg acgagaacgt gatgctgctg acctctgacg cccctgagta caagccttgg
     5701 gctctcgtga tccaggacag caacggcgag aacaagatca agatgctgag cggcggctct
     5761 ggtggctctg gcggatctac aaacctgtcc gatattattg agaaagaaac cgggaaacag
     5821 ctcgtgattc aagagtctat tctcatgctc ccggaagaag tcgaggaagt cattggaaac
     5881 aagcctgaga gcgatattct ggtccataca gcctacgacg agtctaccga tgagaatgtc
     5941 atgctcctca ccagcgacgc tcccgagtat aagccatggg cacttgtcat tcaggactcc
     6001 aatggggaaa acaaaatcaa aatgctccca aagaaaaaac gcaaggtgga gggagctgat
     6061 aagcgcaccg ccgatggttc cgagttcgaa agccccaaga agaagaggaa agtctaaccg
     6121 gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta
     6181 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     6241 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     6301 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     6361 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     6421 gctcgatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     6481 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6541 aagcctaggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6601 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6661 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6721 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6781 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6841 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6901 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6961 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     7021 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     7081 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     7141 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     7201 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     7261 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     7321 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7381 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7441 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7501 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7561 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7621 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7681 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7741 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7801 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7861 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7921 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7981 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     8041 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     8101 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     8161 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     8221 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     8281 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     8341 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8401 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8461 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8521 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8581 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatcgat
     8641 ctcccgatcc cctagggtct actctcagta caatctgctc tgatgccgca tagttaagcc
     8701 agtatctgct ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag
     8761 ctacaacaag gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt
     8821 ttgcgc
//
