LOCUS       pYY_BEM2_26             8958 bp    DNA     circular UNA 22-DEC-2019
DEFINITION  .
ACCESSION   urn.local.yiyu.z1-av9zcz4
VERSION     urn.local.yiyu.z1-av9zcz4
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        35..611
                     /created_by="User"
                     /modified_by="User"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS,pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS,pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS,pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS,pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1(1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B16-ScCas9D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS,pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS ,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS,pYY-B19-Flag-TadA-TadA7.10
                     *-NLS , pYY-B2-SpCas9 D10A-NLS,pYY-B2-SpCas9 D10A-NLS ,
                     pYY-B20-flag-rAPOBEC1-NLS,pYY-B20-flag-rAPOBEC1-NLS ,
                     pYY-B21(flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4(rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1)
                     ,pYY-B23-FLAG-ppBE4 (rAPOBEC1), pYY-B3-SpCas9
                     D10AH840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B4-ScCas9D10A-NLS, pYY-B4-ScCas9 D10A-NLS ,
                     pYY-B5-Sp-ScCas9D10A-NLS, pYY-B5-Sp-ScCas9 D10A-NLS
                     ,pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS
                     ,pYY-B7-SpCas9 D10A-UGI-UGI-NLS,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS ,
                     pYY-B9mutation-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS,pYY-B9-rAPOBEC1-NLS ,
                     pYY-BG34-rAPOBEC1 W90Y R126E-NLS,pYY-BG35 rAPOBEC1 W90Y
                     R126E R132E, pYY-BGX13-hA3A-SpCas9D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl,pYY-BGX27-pmCDA1-SpCas9
                     D10A-UGl, pYY-BGX29-pmCDA5-SpCas9D10A-UGI,
                     pYY-BGX34-rAPOBEC1 W90Y R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BGn-deaminase-NLSCopy, pYY-BX1-pmCDA-SpCas9
                     D10A-UGl,pYY-BX2-TadA-TadA7.10-SpCas9 D10A,
                     pYY-BX3-TadA7.10-SpCas9D10A, pYY-BX4-A3A-SpCas9 D10A-UGl ,
                     pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="pCMV immearly protomter"
     misc_feature    114..282
                     /created_by="User"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS,pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS,pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS,pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS,pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1(1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B16-ScCas9D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS,pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS ,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS,pYY-B19-Flag-TadA-TadA7.10
                     *-NLS , pYY-B2-SpCas9 D10A-NLS,pYY-B2-SpCas9 D10A-NLS ,
                     pYY-B20-flag-rAPOBEC1-NLS,pYY-B20-flag-rAPOBEC1-NLS ,
                     pYY-B21(flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4(rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1)
                     ,pYY-B23-FLAG-ppBE4 (rAPOBEC1), pYY-B3-SpCas9
                     D10AH840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B4-ScCas9D10A-NLS, pYY-B4-ScCas9 D10A-NLS ,
                     pYY-B5-Sp-ScCas9D10A-NLS, pYY-B5-Sp-ScCas9 D10A-NLS
                     ,pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS
                     ,pYY-B7-SpCas9 D10A-UGI-UGI-NLS,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS ,
                     pYY-B9mutation-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS,pYY-B9-rAPOBEC1-NLS ,
                     pYY-BG34-rAPOBEC1 W90Y R126E-NLS,pYY-BG35 rAPOBEC1 W90Y
                     R126E R132E, pYY-BGX13-hA3A-SpCas9D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl,pYY-BGX27-pmCDA1-SpCas9
                     D10A-UGl, pYY-BGX29-pmCDA5-SpCas9D10A-UGI,
                     pYY-BGX34-rAPOBEC1 W90Y R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BGn-deaminase-NLSCopy, pYY-BX1-pmCDA-SpCas9
                     D10A-UGl,pYY-BX2-TadA-TadA7.10-SpCas9 D10A,
                     pYY-BX3-TadA7.10-SpCas9D10A, pYY-BX4-A3A-SpCas9 D10A-UGl ,
                     pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="CAG enhancer"
     primer_bind     294..326
                     /Sequence="ACGCCCCCTATTGACGTCAATGACGGTAAATGG"
                     /Hairpin_Tm="56.5"
                     /Tm="71.1"
                     /Self_Dimer_Tm="30.9"
                     /%GC="51.5"
                     /created_by="primer3"
                     /Transferred_From="pYY-AS22 BE4 Y120A"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-839"
     misc_feature    437..448
                     /Recognition_pattern="(11/13)CAANNNNNGTGG(12/10)"
                     /Transferred_From="pYY-B11-TadA7.10*-NLS,
                     pYY-B11-TadA7.10*-NLS , pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9 D10A-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="CspCI"
                     /note="Geneious type: restriction site"
     promoter        569..638
                     /created_by="User"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS,pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS,pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS,pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS,pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1(1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B16-ScCas9D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS,pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS ,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS,pYY-B19-Flag-TadA-TadA7.10
                     *-NLS , pYY-B2-SpCas9 D10A-NLS,pYY-B2-SpCas9 D10A-NLS ,
                     pYY-B20-flag-rAPOBEC1-NLS,pYY-B20-flag-rAPOBEC1-NLS ,
                     pYY-B21(flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4(rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1)
                     ,pYY-B23-FLAG-ppBE4 (rAPOBEC1), pYY-B3-SpCas9
                     D10AH840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B4-ScCas9D10A-NLS, pYY-B4-ScCas9 D10A-NLS ,
                     pYY-B5-Sp-ScCas9D10A-NLS, pYY-B5-Sp-ScCas9 D10A-NLS
                     ,pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS
                     ,pYY-B7-SpCas9 D10A-UGI-UGI-NLS,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS ,
                     pYY-B9mutation-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS,pYY-B9-rAPOBEC1-NLS ,
                     pYY-BG34-rAPOBEC1 W90Y R126E-NLS,pYY-BG35 rAPOBEC1 W90Y
                     R126E R132E, pYY-BGX13-hA3A-SpCas9D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl,pYY-BGX27-pmCDA1-SpCas9
                     D10A-UGl, pYY-BGX29-pmCDA5-SpCas9D10A-UGI,
                     pYY-BGX34-rAPOBEC1 W90Y R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BGn-deaminase-NLSCopy, pYY-BX1-pmCDA-SpCas9
                     D10A-UGl,pYY-BX2-TadA-TadA7.10-SpCas9 D10A,
                     pYY-BX3-TadA7.10-SpCas9D10A, pYY-BX4-A3A-SpCas9 D10A-UGl ,
                     pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="CMV promoter"
     misc_feature    complement(642..659)
                     /Sequence="AGCGGCCGCGGATCTCTA"
                     /Hairpin_Tm="47.3"
                     /Tm="63.3"
                     /Self_Dimer_Tm="41.7"
                     /%GC="66.7"
                     /created_by="primer3"
                     /Transferred_From="pYY-B12-pmCDA-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-837"
                     /note="Geneious type: primer_bind_reverse"
     Primer          643..662
                     /created_by="yiyu"
                     /Transferred_From="pYY-BGn-deaminase-NLS Copy"
                     /Transferred_Similarity="100.00%"
                     /label="28 B insert FP"
     Primer          complement(643..662)
                     /created_by="yiyu"
                     /modified_by="yiyu"
                     /Transferred_From="pYY-BGn-deaminase-NLS Copy"
                     /Transferred_Similarity="100.00%"
                     /label="29 B BB RP"
     unsure          645..659
                     /created_by="User"
                     /Transferred_From="pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS, pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS, pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS, pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS, pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1 (1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B17 SaCas9 D10A-NLS, pYY-B18-SaCas9
                     D10A-UGI-UGI-NLS, pYY-B18-SaCas9 D10A-UGI-UGI-NLS ,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS , pYY-B2-SpCas9 D10A-NLS,
                     pYY-B2-SpCas9 D10A-NLS , pYY-B20-flag-rAPOBEC1-NLS,
                     pYY-B20-flag-rAPOBEC1-NLS , pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A), pYY-B22-FLAG-BE4
                     (rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1) ,
                     pYY-B23-FLAG-ppBE4 (rAPOBEC1), pYY-B3-SpCas9 D10A
                     H840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS ,
                     pYY-B7-SpCas9 D10A-UGI-UGI-NLS, pYY-B7-SpCas9
                     D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS,
                     pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS , pYY-B9
                     mutation-rAPOBEC1-NLS, pYY-B9-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS , pYY-BG34-rAPOBEC1 W90Y R126E-NLS,
                     pYY-BG35 rAPOBEC1 W90Y R126E R132E, pYY-BGn-deaminase-NLS
                     Copy"
                     /Transferred_Similarity="100.00%"
                     /label="USER"
     unsure          645..>658
                     /created_by="User"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-BGX13-hA3A-SpCas9
                     D10A-UGl,pYY-BGX26-mAID-SpCas9 D10A-UGl,
                     pYY-BGX27-pmCDA1-SpCas9D10A-UGl, pYY-BGX29-pmCDA5-SpCas9
                     D10A-UGI,pYY-BGX34-rAPOBEC1 W90Y
                     R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX2-TadA-TadA7.10-SpCas9
                     D10A,pYY-BX3-TadA7.10-SpCas9 D10A, pYY-BX4-A3A-SpCas9
                     D10A-UGl, pYY-CMn chimeric APOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="USER"
     primer_bind     645..664
                     /Sequence="AGATCCGCGGCCGCTAATAC"
                     /Hairpin_Tm="39.3"
                     /Tm="62.5"
                     /Self_Dimer_Tm="27.0"
                     /%GC="60.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B12-pmCDA-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-836"
     primer_bind     651..677
                     /Sequence="GCGGCCGCTAATACGACTCACTATAGG"
                     /Hairpin_Tm="38.3"
                     /Tm="66.5"
                     /Self_Dimer_Tm="41.7"
                     /%GC="55.6"
                     /modified_by="yiyu"
                     /created_by="primer3"
                     /Transferred_From="pYY-AS22 BE4 Y120A,
                     pYY-BX2-TadA-TadA7.10-SpCas9 D10A, pYY-BX3-TadA7.10-SpCas9
                     D10A"
                     /Transferred_Similarity="100.00%"
                     /label="18 BX1 FP; 21 NotI pmCDA FP; 25 BX3 FP NotI"
     primer_bind     651..675
                     /Sequence="GCGGCCGCTAATACGACTCACTATA"
                     /Hairpin_Tm="38.3"
                     /Tm="64.3"
                     /Self_Dimer_Tm="41.7"
                     /%GC="52.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-BX4-A3A-SpCas9 D10A-UGl"
                     /Transferred_Similarity="100.00%"
                     /label="26 BX4 FP"
     promoter        659..677
                     /created_by="User"
                     /modified_by="yiyu"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS,pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS,pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS,pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS,pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1(1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B16-ScCas9D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS,pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS ,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS,pYY-B19-Flag-TadA-TadA7.10
                     *-NLS , pYY-B2-SpCas9 D10A-NLS,pYY-B2-SpCas9 D10A-NLS ,
                     pYY-B20-flag-rAPOBEC1-NLS,pYY-B20-flag-rAPOBEC1-NLS ,
                     pYY-B21(flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4(rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1)
                     ,pYY-B23-FLAG-ppBE4 (rAPOBEC1), pYY-B3-SpCas9
                     D10AH840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B4-ScCas9D10A-NLS, pYY-B4-ScCas9 D10A-NLS ,
                     pYY-B5-Sp-ScCas9D10A-NLS, pYY-B5-Sp-ScCas9 D10A-NLS
                     ,pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS
                     ,pYY-B7-SpCas9 D10A-UGI-UGI-NLS,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS ,
                     pYY-B9mutation-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS,pYY-B9-rAPOBEC1-NLS ,
                     pYY-BG34-rAPOBEC1 W90Y R126E-NLS,pYY-BG35 rAPOBEC1 W90Y
                     R126E R132E, pYY-BGX13-hA3A-SpCas9D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl,pYY-BGX27-pmCDA1-SpCas9
                     D10A-UGl, pYY-BGX29-pmCDA5-SpCas9D10A-UGI,
                     pYY-BGX34-rAPOBEC1 W90Y R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BGn-deaminase-NLSCopy, pYY-BX1-pmCDA-SpCas9
                     D10A-UGl,pYY-BX2-TadA-TadA7.10-SpCas9 D10A,
                     pYY-BX3-TadA7.10-SpCas9D10A, pYY-BX4-A3A-SpCas9 D10A-UGl ,
                     pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="T7 promoter; T7 protomet"
     primer_bind     659..678
                     /Sequence="TAATACGACTCACTATAGGG"
                     /Hairpin_Tm="None"
                     /Tm="50.3"
                     /Self_Dimer_Tm="None"
                     /%GC="40.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-AS22 BE4 Y120A"
                     /Transferred_Similarity="100.00%"
                     /label="T7"
     primer_bind     complement(669..690)
                     /Sequence="GGTGGCGGCTCTCCCTATAGTG"
                     /Hairpin_Tm="None"
                     /Tm="64.2"
                     /Self_Dimer_Tm="11.4"
                     /%GC="63.6"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A), pYY-B23-FLAG-ppBE4
                     (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="54 B21 RP; B21 RP"
     Target_site     complement(672..690)
                     /created_by="yiyu"
                     /modified_by="yiyu"
                     /Transferred_From="pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS, pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS, pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS, pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS, pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1 (1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B19-Flag-TadA-TadA7.10*-NLS,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS , pYY-B2-SpCas9 D10A-NLS,
                     pYY-B2-SpCas9 D10A-NLS , pYY-B20-flag-rAPOBEC1-NLS,
                     pYY-B20-flag-rAPOBEC1-NLS , pYY-B3-SpCas9 D10A H840A-NLS,
                     pYY-B3-SpCas9 D10A H840A-NLS , pYY-B9
                     mutation-rAPOBEC1-NLS, pYY-B9-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS , pYY-BG34-rAPOBEC1 W90Y R126E-NLS,
                     pYY-BG35 rAPOBEC1 W90Y R126E R132E, pYY-BGn-deaminase-NLS
                     Copy, pYY-BX4-A3A-SpCas9 D10A-UGl"
                     /Transferred_Similarity="100.00%"
                     /label="1 pCMV BB RP"
     primer_bind     complement(672..690)
                     /Mismatches=0
                     /%GC="63.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="11.4"
                     /Tm="60.5"
                     /Sequence="GGTGGCGGCTCTCCCTATA"
                     /created_by="primer3"
                     /Transferred_From="pYY-B18-SaCas9 D10A-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="1 pCMV BB RP"
     Primer          complement(1399..1416)
                     /created_by="yiyu"
                     /modified_by="yiyu"
                     /Transferred_From="pYY-BGn-deaminase-NLS Copy"
                     /Transferred_Similarity="100.00%"
                     /label="31 insert RP"
     CDS             1405..1500
                     /created_by="ngaudelli"
                     /created_by="ngaudelli"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B22-FLAG-BE4 (rAPOBEC1),
                     pYY-B22-FLAG-BE4(rAPOBEC1) , pYY-B23-FLAG-ppBE4
                     (rAPOBEC1),pYY-BGX13-hA3A-SpCas9 D10A-UGl,
                     pYY-BGX26-mAID-SpCas9D10A-UGl, pYY-BGX27-pmCDA1-SpCas9
                     D10A-UGl,pYY-BGX29-pmCDA5-SpCas9 D10A-UGI,
                     pYY-BGX34-rAPOBEC1 W90YR126E-UGl, pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl ,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX2-TadA-TadA7.10-SpCas9
                     D10A,pYY-BX3-TadA7.10-SpCas9 D10A, pYY-BX4-A3A-SpCas9
                     D10A-UGl, pYY-CMn chimeric APOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="linker"
     CDS             1405..>1416
                     /created_by="ngaudelli"
                     /Transferred_From="pYY-BGn-deaminase-NLS Copy"
                     /Transferred_Similarity="100.00%"
                     /label="linker"
     primer_bind     complement(1478..1522)
                     /Sequence="GGCCGATGCTGTACTTCTTGTCAGAGCCACCAGAAGATCCGCCGC"
                     /created_by="yiyu"
                     /Transferred_From="pYY-B23-FLAG-ppBE4 (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="57 B22 insert RP"
     misc_feature    complement(1479..1498)
                     /Sequence="AGCCACCAGAAGATCCGCCG"
                     /Hairpin_Tm="None"
                     /Tm="64.7"
                     /Self_Dimer_Tm="None"
                     /%GC="65.0"
                     /created_by="primer3"
                     /modified_by="ngaudelli"
                     /Transferred_From="pYY-AS22 BE4 Y120A"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B140"
                     /note="Geneious type: primer_bind_reverse"
     unsure          1485..1498
                     /created_by="ngaudelli"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-BGX13-hA3A-SpCas9
                     D10A-UGl,pYY-BGX26-mAID-SpCas9 D10A-UGl,
                     pYY-BGX27-pmCDA1-SpCas9D10A-UGl, pYY-BGX29-pmCDA5-SpCas9
                     D10A-UGI,pYY-BGX34-rAPOBEC1 W90Y
                     R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl ,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX2-TadA-TadA7.10-SpCas9
                     D10A,pYY-BX3-TadA7.10-SpCas9 D10A, pYY-BX4-A3A-SpCas9
                     D10A-UGl, pYY-CMn chimeric APOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="USER"
     unsure          1485..1498
                     /created_by="ngaudelli"
                     /Transferred_From="pYY-B22-FLAG-BE4 (rAPOBEC1),
                     pYY-B22-FLAG-BE4 (rAPOBEC1) , pYY-B23-FLAG-ppBE4
                     (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="USER"
     primer_bind     1485..1521
                     /Sequence="ATCTTCTGGTGGCTCTGACAAGAAGTACAGCATCGGC"
                     /created_by="nicolegaudelli"
                     /Transferred_From="pYY-AS22 BE4 Y120A"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B235"
     USER            <1501..>1510
                     /created_by="Yi Yu"
                     /modified_by="Yi Yu"
                     /Transferred_From="pYY-B16-ScCas9 D10A R211K, N404K-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="USER1-A03"
     CDS             1501..5601
                     /note="hSpCas9"
                     /modified_by="jonlevy"
                     /modified_by="jonlevy"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A),pYY-BGX13-hA3A-SpCas9
                     D10A-UGl, pYY-BGX26-mAID-SpCas9D10A-UGl,
                     pYY-BGX27-pmCDA1-SpCas9 D10A-UGl,pYY-BGX29-pmCDA5-SpCas9
                     D10A-UGI, pYY-BGX34-rAPOBEC1 W90YR126E-UGl,
                     pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX4-A3A-SpCas9 D10A-UGl
                     , pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="fz hSpCas9"
     Primer          <1501..1522
                     /created_by="Yi Yu"
                     /Transferred_From="pYY-B16-ScCas9 D10A R211K, N404K-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="YY-AP8 hSpCas9 FP"
     Primer          complement(<1501..>1510)
                     /created_by="Yi Yu"
                     /modified_by="Yi Yu"
                     /Transferred_From="pYY-B16-ScCas9 D10A R211K, N404K-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="YY-AP7 A03 BB RP"
     primer_bind     1501..1522
                     /Sequence="GACAAGAAGTACAGCATCGGCC"
                     /Hairpin_Tm="42.6"
                     /Tm="61.6"
                     /Self_Dimer_Tm="None"
                     /%GC="54.5"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A), pYY-B23-FLAG-ppBE4
                     (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="55 B21 FP; 55 b21 FP"
     primer_bind     1502..1527
                     /Sequence="ACAAGAAGTACAGCATCGGCCTGGCC"
                     /Hairpin_Tm="46.8"
                     /Tm="68.9"
                     /Self_Dimer_Tm="28.2"
                     /%GC="57.7"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="KZ495"
     USER            1513..1523
                     /created_by="nicolegaudelli"
                     /Transferred_From="pYY-B1-SpCas9-NLS, pYY-B2-SpCas9
                     D10A-NLS, pYY-B2-SpCas9 D10A-NLS , pYY-B3-SpCas9 D10A
                     H840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS ,
                     pYY-B7-SpCas9 D10A-UGI-UGI-NLS, pYY-B7-SpCas9
                     D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS,
                     pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
     primer_bind     complement(1578..1597)
                     /Sequence="CCTTGAATTTCTTGCTGGGC"
                     /Hairpin_Tm="None"
                     /Tm="57.6"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl"
                     /Transferred_Similarity="100.00%"
                     /label="SP62"
     misc_feature    complement(1622..1642)
                     /Sequence="CGATCAGGTTCTTCTTGATGC"
                     /Hairpin_Tm="41.8"
                     /Tm="57.1"
                     /Self_Dimer_Tm="None"
                     /%GC="47.6"
                     /created_by="primer3"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B272"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     1622..1642
                     /Sequence="GCATCAAGAAGAACCTGATCG"
                     /Hairpin_Tm="None"
                     /Tm="57.1"
                     /Self_Dimer_Tm="None"
                     /%GC="47.6"
                     /created_by="primer3"
                     /X-Transferred_From="LCv2_seq10"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A), pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq10; NMG-B23"
     primer_bind     1650..1669
                     /Sequence="GCTGTTCGACAGCGGCGAAA"
                     /Hairpin_Tm="58.0"
                     /Tm="64.2"
                     /Self_Dimer_Tm="18.3"
                     /%GC="60.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="A03-Seq2000"
     Primer          1748..1769
                     /created_by="yiyu"
                     /modified_by="yiyu"
                     /Transferred_From="pYY-B16-ScCas9 D10A R211K, N404K-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="105 G7 BglII FP"
     Primer          complement(1754..1773)
                     /created_by="yiyu"
                     /modified_by="yiyu"
                     /Transferred_From="pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl"
                     /Transferred_Similarity="100.00%"
                     /label="45 BGYn"
     misc_feature    1791..1801
                     /Recognition_pattern="CCANNNN^NTGG"
                     /annotation_group="CCANNNNNTGG"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS, pYY-B2-SpCas9
                     D10A-NLS,pYY-B2-SpCas9 D10A-NLS , pYY-B3-SpCas9 D10A
                     H840A-NLS,pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B6-SpCas9-UGI-UGI-NLS,pYY-B6-SpCas9-UGI-UGI-NLS ,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS, pYY-B7-SpCas9
                     D10A-UGI-UGI-NLS ,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS,
                     pYY-B8-SpCas9 D10AH840-UGI-UGI-NLS , pYY-BGX13-hA3A-SpCas9
                     D10A-UGl,pYY-BGX26-mAID-SpCas9 D10A-UGl,
                     pYY-BGX27-pmCDA1-SpCas9D10A-UGl, pYY-BGX29-pmCDA5-SpCas9
                     D10A-UGI,pYY-BGX34-rAPOBEC1 W90Y
                     R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX2-TadA-TadA7.10-SpCas9
                     D10A,pYY-BX3-TadA7.10-SpCas9 D10A, pYY-BX4-A3A-SpCas9
                     D10A-UGl, pYY-CMn chimeric APOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="PflMI"
                     /note="Geneious type: restriction site"
     misc_feature    1791..1801
                     /Recognition_pattern="CCANNNN^NTGG"
                     /Transferred_From="pYY-B16-ScCas9 D10A R211K, N404K-NLS,
                     pYY-B21 (flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4 (rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1) ,
                     pYY-B23-FLAG-ppBE4 (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="PflMI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(1928..1947)
                     /Sequence="GTCGGCCTTGTCGGTGCTGT"
                     /Hairpin_Tm="34.3"
                     /Tm="65.4"
                     /Self_Dimer_Tm="None"
                     /%GC="65.0"
                     /created_by="primer3"
                     /modified_by="nicolegaudelli"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B290"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     1928..1947
                     /Sequence="ACAGCACCGACAAGGCCGAC"
                     /Hairpin_Tm="46.5"
                     /Tm="65.4"
                     /Self_Dimer_Tm="None"
                     /%GC="65.0"
                     /modified_by="nicolegaudelli"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B289"
     primer_bind     2074..2093
                     /Sequence="AACCAGCTGTTCGAGGAAAA"
                     /Hairpin_Tm="28.5"
                     /Tm="57.7"
                     /Self_Dimer_Tm="13.8"
                     /%GC="45.0"
                     /created_by="primer3"
                     /X-Transferred_From="LCv2_seq11"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A), pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq11; NMG-B24"
     primer_bind     2150..2169
                     /Sequence="AGAGCAGACGGCTGGAAAAT"
                     /Hairpin_Tm="57.6"
                     /Tm="59.7"
                     /Self_Dimer_Tm="22.3"
                     /%GC="50.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="A03-Seq2500; A03-Seq2500"
     primer_bind     2626..2645
                     /Sequence="AAGCCCATCCTGGAAAAGAT"
                     /X-Transferred_From="LCv2_seq12"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="LCv2_seq12"
                     /Transferred_Similarity="100.00%"
                     /Hairpin_Tm="41.0"
                     /Tm="56.8"
                     /Self_Dimer_Tm="None"
                     /%GC="45.0"
                     /created_by="primer3"
                     /modified_by="nicolegaudelli"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-B23-FLAG-ppBE4
                     (rAPOBEC1), pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq12; NMG-B25"
     primer_bind     2650..2669
                     /Sequence="GGCACCGAGGAACTGCTCGT"
                     /Hairpin_Tm="41.6"
                     /Tm="64.9"
                     /Self_Dimer_Tm="17.6"
                     /%GC="65.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="A03-Seq3000; A03-Seq3000"
     Primer          complement(2841..2858)
                     /created_by="yiyu"
                     /Transferred_From="pYY-B16-ScCas9 D10A R211K, N404K-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="106 G7 ApaI RP"
     misc_feature    complement(3039..3051)
                     /Recognition_pattern="(7/12)GAAGNNNNNNTAC(12/7)"
                     /Transferred_From="pYY-B16-ScCas9 D10A R211K, N404K-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="BarI"
                     /note="Geneious type: restriction site"
     primer_bind     3107..3126
                     /Sequence="CCGCCTTCCTGAGCGGCGAG"
                     /Hairpin_Tm="62.5"
                     /Tm="68.0"
                     /Self_Dimer_Tm="24.3"
                     /%GC="75.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B26"
     primer_bind     3120..3139
                     /Sequence="CGGCGAGCAGAAAAAGGCCA"
                     /Hairpin_Tm="40.5"
                     /Tm="64.0"
                     /Self_Dimer_Tm="5.0"
                     /%GC="60.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B4-ScCas9 D10A-NLS, pYY-B4-ScCas9
                     D10A-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="A01-Seq3500; A01-Seq3500"
     primer_bind     3125..3143
                     /Sequence="AGCAGAAAAAGGCCATCGT"
                     /Transferred_From="LCv2_seq13"
                     /Transferred_Similarity="100.00%"
                     /X-Transferred_From="LCv2_seq13"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-B23-FLAG-ppBE4
                     (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq13"
     primer_bind     3150..3169
                     /Sequence="GCTGTTCAAGACCAACCGGA"
                     /Hairpin_Tm="35.6"
                     /Tm="60.5"
                     /Self_Dimer_Tm="9.5"
                     /%GC="55.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="A03-Seq3500"
     primer_bind     complement(3186..3236)
                     /Sequence="ATTTCCACGGAGTCGAAGCACTCGATTTTCTTGAAGTAGTCCTCTTT
                     CAGC"
                     /%GC="45.1"
                     /Transferred_From="876_fzCas9-resplice"
                     /Transferred_Similarity="100.00%"
                     /X-Transferred_From="876_fzCas9-resplice"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-B23-FLAG-ppBE4
                     (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="876_fzCas9-resplice"
     primer_bind     complement(3186..3216)
                     /Sequence="CTCGATTTTCTTGAAGTAGTCCTCTTTCAGC"
                     /%GC="41.9"
                     /X-Transferred_From="901_fzCas9_NpuC-rev"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="901_fzCas9_NpuC-rev"
                     /Transferred_Similarity="100.00%"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-B23-FLAG-ppBE4
                     (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="901_fzCas9_NpuC-rev"
     primer_bind     3217..3239
                     /Sequence="TGCTTCGACTCCGTGGAAATCTC"
                     /%GC="52.2"
                     /X-Transferred_From="841_FZ_Cas9-C11-fwd"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="841_FZ_Cas9-C11-fwd"
                     /Transferred_Similarity="100.00%"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-B23-FLAG-ppBE4
                     (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="841_FZ_Cas9-C11-fwd"
     Primer          complement(3369..3392)
                     /created_by="Yi Yu"
                     /Transferred_From="pYY-B4-ScCas9 D10A-NLS, pYY-B4-ScCas9
                     D10A-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="YYA-P5 hScCas9A RP"
     USER            3380..3392
                     /created_by="Yi Yu"
                     /Transferred_From="pYY-B4-ScCas9 D10A-NLS, pYY-B4-ScCas9
                     D10A-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="USER3-A01"
     Primer          3380..3402
                     /created_by="Yi Yu"
                     /modified_by="Yi Yu"
                     /Transferred_From="pYY-B4-ScCas9 D10A-NLS, pYY-B4-ScCas9
                     D10A-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="YYA-P6 hScCas9B FP"
     primer_bind     3619..3638
                     /Sequence="ATCCAGAAAGCCCAGGTGTC"
                     /Transferred_From="LCv2_seq14"
                     /Transferred_Similarity="100.00%"
                     /X-Transferred_From="LCv2_seq14"
                     /X-Transferred_Similarity="100.00%"
                     /Hairpin_Tm="None"
                     /Tm="59.7"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-B23-FLAG-ppBE4
                     (rAPOBEC1), pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq14; NMG-B159"
     primer_bind     3650..3669
                     /Sequence="ATAGCCTGCACGAGCACATT"
                     /Hairpin_Tm="48.1"
                     /Tm="60.1"
                     /Self_Dimer_Tm="2.5"
                     /%GC="50.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="A03-Seq4000"
     misc_feature    3972..3982
                     /Recognition_pattern="CCANNNN^NTGG"
                     /annotation_group="CCANNNNNTGG"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS, pYY-B2-SpCas9
                     D10A-NLS,pYY-B2-SpCas9 D10A-NLS , pYY-B3-SpCas9 D10A
                     H840A-NLS,pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B6-SpCas9-UGI-UGI-NLS,pYY-B6-SpCas9-UGI-UGI-NLS ,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS, pYY-B7-SpCas9
                     D10A-UGI-UGI-NLS ,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS,
                     pYY-B8-SpCas9 D10AH840-UGI-UGI-NLS , pYY-BGX13-hA3A-SpCas9
                     D10A-UGl,pYY-BGX26-mAID-SpCas9 D10A-UGl,
                     pYY-BGX27-pmCDA1-SpCas9D10A-UGl, pYY-BGX29-pmCDA5-SpCas9
                     D10A-UGI,pYY-BGX34-rAPOBEC1 W90Y
                     R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX2-TadA-TadA7.10-SpCas9
                     D10A,pYY-BX3-TadA7.10-SpCas9 D10A, pYY-BX4-A3A-SpCas9
                     D10A-UGl, pYY-CMn chimeric APOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="PflMI"
                     /note="Geneious type: restriction site"
     misc_feature    3972..3982
                     /Recognition_pattern="CCANNNN^NTGG"
                     /Transferred_From="pYY-B16-ScCas9 D10A R211K, N404K-NLS,
                     pYY-B21 (flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4 (rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1) ,
                     pYY-B23-FLAG-ppBE4 (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="PflMI"
                     /note="Geneious type: restriction site"
     primer_bind     4095..4114
                     /Sequence="GAGCGACAACGTGCCCTCCG"
                     /Hairpin_Tm="42.0"
                     /Tm="66.1"
                     /Self_Dimer_Tm="None"
                     /%GC="70.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B27"
     primer_bind     4112..4131
                     /Sequence="CCGAAGAGGTCGTGAAGAAG"
                     /Transferred_From="LCv2_seq15"
                     /Transferred_Similarity="100.00%"
                     /X-Transferred_From="LCv2_seq15"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-B23-FLAG-ppBE4
                     (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq15"
     primer_bind     4150..4169
                     /Sequence="CAGCTGCTGAACGCCAAGCT"
                     /Hairpin_Tm="45.7"
                     /Tm="64.0"
                     /Self_Dimer_Tm="24.9"
                     /%GC="60.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="A03-Seq4500; A03-Seq4500"
     misc_feature    4314..4326
                     /Recognition_pattern="(7/12)GAACNNNNNNTAC(12/7)"
                     /Transferred_From="pYY-B16-ScCas9 D10A R211K, N404K-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="PsrI"
                     /note="Geneious type: restriction site"
     primer_bind     4527..4546
                     /Sequence="CGGCGACTACAAGGTGTACG"
                     /Hairpin_Tm="None"
                     /Tm="60.8"
                     /Self_Dimer_Tm="None"
                     /%GC="60.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B28"
     primer_bind     4623..4642
                     /Sequence="CATGAACTTTTTCAAGACCG"
                     /Hairpin_Tm="45.0"
                     /Tm="53.1"
                     /Self_Dimer_Tm="None"
                     /%GC="40.0"
                     /modified_by="Yi Yu"
                     /Transferred_From="pYY-B4-ScCas9 D10A-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="A01-Seq5000"
     primer_bind     4642..4666
                     /Sequence="GAGATTACCCTGGCCAACGGCGAGA"
                     /Hairpin_Tm="53.7"
                     /Tm="68.3"
                     /Self_Dimer_Tm="16.6"
                     /%GC="60.0"
                     /created_by="primer3"
                     /modified_by="nicolegaudelli"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B274"
     primer_bind     4650..4669
                     /Sequence="CCTGGCCAACGGCGAGATCC"
                     /Hairpin_Tm="53.7"
                     /Tm="65.6"
                     /Self_Dimer_Tm="16.6"
                     /%GC="70.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="A03-Seq5000; A03-Seq5000"
     Primer          complement(4769..>4794)
                     /created_by="Yi Yu"
                     /modified_by="Yi Yu"
                     /Transferred_From="pYY-B16-ScCas9 D10A R211K, N404K-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="YY-AP12 hSpCas9 minus PI RP"
     misc_feature    complement(4817..4841)
                     /Sequence="TTCCTCTTGGGCAGGATAGACTCTT"
                     /Hairpin_Tm="39.9"
                     /Tm="62.8"
                     /Self_Dimer_Tm="None"
                     /%GC="48.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B275"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(4876..4899)
                     /Sequence="GAAGCCGCCGTACTTCTTAGGGTC"
                     /Hairpin_Tm="39.4"
                     /Tm="65.0"
                     /Self_Dimer_Tm="9.2"
                     /%GC="58.3"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-1606"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     4903..4924
                     /Sequence="AGCCCCACCGTGGCCTATTCTG"
                     /Hairpin_Tm="50.8"
                     /Tm="66.6"
                     /Self_Dimer_Tm="12.5"
                     /%GC="63.6"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-1605"
     misc_feature    4992..5001
                     /Recognition_pattern="GATNN^NNATC"
                     /Transferred_From="pYY-B1-SpCas9-NLS, pYY-B2-SpCas9
                     D10A-NLS, pYY-B2-SpCas9 D10A-NLS , pYY-B3-SpCas9 D10A
                     H840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS ,
                     pYY-B7-SpCas9 D10A-UGI-UGI-NLS, pYY-B7-SpCas9
                     D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS,
                     pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS ,
                     pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl"
                     /Transferred_Similarity="100.00%"
                     /label="BsaBI, Bse8I, BseJI"
                     /note="Geneious type: restriction site"
     primer_bind     5074..5093
                     /Sequence="GACCTGATCATCAAGCTGCC"
                     /Hairpin_Tm="None"
                     /Tm="58.7"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B29"
     misc_feature    complement(5127..5144)
                     /Sequence="GAGGCCAGCATTCTCTTC"
                     /Hairpin_Tm="None"
                     /Tm="55.1"
                     /Self_Dimer_Tm="5.0"
                     /%GC="55.6"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-1608"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     5150..5169
                     /created_by="Yi Yu; primer3"
                     /Sequence="GCGAACTGCAGAAGGGAAAC"
                     /Hairpin_Tm="45.5"
                     /Tm="59.8"
                     /Self_Dimer_Tm="23.4"
                     /%GC="55.0"
                     /modified_by="Yi Yu"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="A03-Seq5500; A03-Seq5500"
     primer_bind     5152..5176
                     /Sequence="GAACTGCAGAAGGGAAACGAACTGG"
                     /Hairpin_Tm="None"
                     /Tm="64.2"
                     /Self_Dimer_Tm="23.4"
                     /%GC="52.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-1607"
     primer_bind     5252..5278
                     /Sequence="ATGAGCAGAAACAGCTGTTTGTGGAAC"
                     /Hairpin_Tm="50.2"
                     /Tm="64.5"
                     /Self_Dimer_Tm="44.5"
                     /%GC="44.4"
                     /created_by="primer3"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B277"
     misc_feature    complement(5330..5354)
                     /Sequence="TTAGCGTCGGCCAGGATCACTCTCT"
                     /Hairpin_Tm="41.2"
                     /Tm="67.1"
                     /Self_Dimer_Tm="2.2"
                     /%GC="56.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B278"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(5478..5499)
                     /Sequence="CTTCCGGTCGATGGTGGTGTCA"
                     /Hairpin_Tm="None"
                     /Tm="64.5"
                     /Self_Dimer_Tm="None"
                     /%GC="59.1"
                     /created_by="primer3"
                     /modified_by="ngaudelli"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-1610"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    5509..5529
                     /Sequence="AGCACCAAAGAGGTGCTGGAC"
                     /Hairpin_Tm="77.5"
                     /Tm="62.9"
                     /Self_Dimer_Tm="33.6"
                     /%GC="57.1"
                     /created_by="primer3"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-1609 (VRER)"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     5568..5587
                     /Sequence="GACACGGATCGACCTGTCTC"
                     /Hairpin_Tm="40.4"
                     /Tm="59.9"
                     /Self_Dimer_Tm="18.1"
                     /%GC="60.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B30"
     misc_feature    complement(5579..5622)
                     /Sequence="AGATCCTCCGCTTCCACCAGAGTCACCTCCCAGCTGAGACAGGT"
                     /created_by="nicolegaudelli"
                     /Transferred_From="pYY-B23-FLAG-ppBE4 (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B291"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(5583..5620)
                     /created_by="yiyu"
                     /Sequence="ATCCTCCGCTTCCACCAGAGTCACCTCCCAGCTGAGAC"
                     /modified_by="yiyu"
                     /Transferred_From="pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="11 B6 RP"
     unsure          <5602..>6159
                     /created_by="ngaudelli"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS , pYY-B22-FLAG-BE4
                     (rAPOBEC1),pYY-B22-FLAG-BE4 (rAPOBEC1) ,
                     pYY-B23-FLAG-ppBE4(rAPOBEC1),
                     pYY-B6-SpCas9-UGI-UGI-NLS,pYY-B6-SpCas9-UGI-UGI-NLS ,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS, pYY-B7-SpCas9
                     D10A-UGI-UGI-NLS ,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS,
                     pYY-B8-SpCas9 D10AH840-UGI-UGI-NLS ,
                     pYY-BGX27-pmCDA1-SpCas9 D10A-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX4-A3A-SpCas9 D10A-UGl
                     , pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="gene block; gene block"
     unsure          <5602..>5631
                     /created_by="ngaudelli"
                     /Transferred_From="pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl ,
                     pYY-BX2-TadA-TadA7.10-SpCas9 D10A, pYY-BX3-TadA7.10-SpCas9
                     D10A"
                     /Transferred_Similarity="100.00%"
                     /label="gene block"
     CDS             5602..6159
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS , pYY-B22-FLAG-BE4
                     (rAPOBEC1),pYY-B22-FLAG-BE4 (rAPOBEC1) ,
                     pYY-B23-FLAG-ppBE4(rAPOBEC1),
                     pYY-B6-SpCas9-UGI-UGI-NLS,pYY-B6-SpCas9-UGI-UGI-NLS ,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS, pYY-B7-SpCas9
                     D10A-UGI-UGI-NLS ,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS,
                     pYY-B8-SpCas9 D10AH840-UGI-UGI-NLS ,
                     pYY-BGX27-pmCDA1-SpCas9 D10A-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX4-A3A-SpCas9 D10A-UGl
                     , pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="ORF1"
     misc_feature    complement(5602..5622)
                     /Sequence="AGATCCTCCGCTTCCACCAGA"
                     /Hairpin_Tm="None"
                     /Tm="62.7"
                     /Self_Dimer_Tm="None"
                     /%GC="57.1"
                     /modified_by="nicolegaudelli"
                     /Transferred_From="pYY-BX2-TadA-TadA7.10-SpCas9 D10A"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B363"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     5602..5620
                     /Sequence="TCTGGTGGAAGCGGAGGAT"
                     /Hairpin_Tm="46.3"
                     /Tm="60.3"
                     /Self_Dimer_Tm="None"
                     /%GC="57.9"
                     /modified_by="yiyu"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-B18-SaCas9
                     D10A-UGI-UGI-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="10 pCMV BB UGI FP; 10 pCMV BB UGI FP"
     USER            5610..5622
                     /created_by="ngaudelli"
                     /Transferred_From="pYY-B22-FLAG-BE4 (rAPOBEC1),
                     pYY-B22-FLAG-BE4 (rAPOBEC1) , pYY-B23-FLAG-ppBE4
                     (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
     USER            5610..5622
                     /created_by="ngaudelli"
                     /Transferred_From="pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl ,
                     pYY-BX2-TadA-TadA7.10-SpCas9 D10A, pYY-BX3-TadA7.10-SpCas9
                     D10A"
                     /Transferred_Similarity="100.00%"
     primer_bind     5610..5631
                     /Sequence="AAGCGGAGGATCTGGCGGCAGC"
                     /Hairpin_Tm="43.4"
                     /Tm="69.6"
                     /Self_Dimer_Tm="32.4"
                     /%GC="68.2"
                     /created_by="primer3"
                     /Transferred_From="pYY-B23-FLAG-ppBE4 (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B225"
     CDS             5611..>5622
                     /created_by="ngaudelli"
                     /Transferred_From="pYY-BGn-deaminase-NLS Copy"
                     /Transferred_Similarity="100.00%"
                     /label="linker"
     unsure          <5632..>6159
                     /created_by="ngaudelli"
                     /Transferred_From="pYY-BGX13-hA3A-SpCas9 D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl, pYY-BGX29-pmCDA5-SpCas9
                     D10A-UGI, pYY-BGX34-rAPOBEC1 W90Y R126E-UGl"
                     /Transferred_Similarity="100.00%"
                     /label="gene block"
     CDS             <5632..6159
                     /Transferred_From="pYY-BGX13-hA3A-SpCas9 D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl, pYY-BGX29-pmCDA5-SpCas9
                     D10A-UGI, pYY-BGX34-rAPOBEC1 W90Y R126E-UGl"
                     /Transferred_Similarity="100.00%"
                     /label="ORF1"
     misc_feature    complement(6128..6158)
                     /Sequence="AGCATTTTGATTTTGTTTTCCCCATTGGAGT"
                     /Hairpin_Tm="46.2"
                     /Tm="64.9"
                     /Self_Dimer_Tm="3.6"
                     /%GC="35.5"
                     /created_by="primer3"
                     /Transferred_From="pYY-AS22 BE4 Y120A"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B144"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(6138..6179)
                     /Sequence="ACCTTGCGTTTTTTCTTTGGGAGCATTTTGATTTTGTTTTCC"
                     /created_by="ngaudelli"
                     /Transferred_From="pYY-B23-FLAG-ppBE4 (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B224"
                     /note="Geneious type: primer_bind_reverse"
     CDS             <6160..6250
                     /created_by="nicolegaudelli"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-BGX13-hA3A-SpCas9
                     D10A-UGl,pYY-BGX26-mAID-SpCas9 D10A-UGl,
                     pYY-BGX27-pmCDA1-SpCas9D10A-UGl, pYY-BGX29-pmCDA5-SpCas9
                     D10A-UGI,pYY-BGX34-rAPOBEC1 W90Y
                     R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX4-A3A-SpCas9 D10A-UGl
                     , pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="NLS tag"
     CDS             6160..6249
                     /created_by="yiyu"
                     /modified_by="yiyu"
                     /Transferred_From="pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS, pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS, pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS, pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS, pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1 (1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B16-ScCas9 D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS, pYY-B18-SaCas9 D10A-UGI-UGI-NLS, pYY-B18-SaCas9
                     D10A-UGI-UGI-NLS , pYY-B19-Flag-TadA-TadA7.10*-NLS,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS , pYY-B2-SpCas9 D10A-NLS,
                     pYY-B2-SpCas9 D10A-NLS , pYY-B20-flag-rAPOBEC1-NLS,
                     pYY-B20-flag-rAPOBEC1-NLS , pYY-B22-FLAG-BE4 (rAPOBEC1),
                     pYY-B22-FLAG-BE4 (rAPOBEC1) , pYY-B23-FLAG-ppBE4
                     (rAPOBEC1), pYY-B3-SpCas9 D10A H840A-NLS, pYY-B3-SpCas9
                     D10A H840A-NLS , pYY-B4-ScCas9 D10A-NLS, pYY-B4-ScCas9
                     D10A-NLS , pYY-B5-Sp-ScCas9 D10A-NLS, pYY-B5-Sp-ScCas9
                     D10A-NLS , pYY-B6-SpCas9-UGI-UGI-NLS,
                     pYY-B6-SpCas9-UGI-UGI-NLS , pYY-B7-SpCas9
                     D10A-UGI-UGI-NLS, pYY-B7-SpCas9 D10A-UGI-UGI-NLS ,
                     pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS, pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS , pYY-B9 mutation-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS, pYY-B9-rAPOBEC1-NLS ,
                     pYY-BG34-rAPOBEC1 W90Y R126E-NLS, pYY-BG35 rAPOBEC1 W90Y
                     R126E R132E, pYY-BGn-deaminase-NLS Copy"
                     /Transferred_Similarity="100.00%"
                     /label="BPNLS"
     primer_bind     6160..6182
                     /Mismatches=0
                     /%GC="43.5"
                     /Hairpin_Tm="46.4"
                     /Self_Dimer_Tm="None"
                     /Tm="60.7"
                     /Sequence="CCAAAGAAAAAACGCAAGGTGGA"
                     /created_by="primer3"
                     /Transferred_From="pYY-B12-pmCDA-NLS, pYY-B23-FLAG-ppBE4
                     (rAPOBEC1)"
                     /Transferred_Similarity="100.00%"
                     /label="2 pCMV BB NLS FP"
     primer_bind     <6163..6183
                     /created_by="nicolegaudelli"
                     /Sequence="ACAAGCCCAAGCTGGACTTCCCAAAGAAAAAACGCAAGGTGGAG"
                     /modified_by="nicolegaudelli"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B354"
     USER            6167..6179
                     /modified_by="ngaudelli"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-BGX13-hA3A-SpCas9
                     D10A-UGl,pYY-BGX26-mAID-SpCas9 D10A-UGl,
                     pYY-BGX27-pmCDA1-SpCas9D10A-UGl, pYY-BGX29-pmCDA5-SpCas9
                     D10A-UGI,pYY-BGX34-rAPOBEC1 W90Y
                     R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX2-TadA-TadA7.10-SpCas9
                     D10A,pYY-BX3-TadA7.10-SpCas9 D10A, pYY-BX4-A3A-SpCas9
                     D10A-UGl, pYY-CMn chimeric APOBECs"
                     /Transferred_Similarity="100.00%"
     USER            6167..6179
                     /modified_by="Yi Yu"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="USER2-A03"
     Primer          <6167..6187
                     /created_by="Yi Yu"
                     /modified_by="Yi Yu"
                     /Transferred_From="pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="YYA-P9 A03 BB FP"
     primer_bind     6167..6213
                     /Sequence="AAAAACGCAAGGTGGAGGGAGCTGATAAGCGCACCGCCGATGGTTCC
                     "
                     /created_by="ngaudelli"
                     /created_by="ngaudelli"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A)"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-B223"
     source          <6181..>6192
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /modified_by="hrees"
                     /modified_by="hrees"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS,pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS,pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS,pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS,pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1(1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B16-ScCas9D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS,pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS ,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS,pYY-B19-Flag-TadA-TadA7.10
                     *-NLS , pYY-B2-SpCas9 D10A-NLS,pYY-B2-SpCas9 D10A-NLS ,
                     pYY-B20-flag-rAPOBEC1-NLS,pYY-B20-flag-rAPOBEC1-NLS ,
                     pYY-B21(flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4(rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1)
                     ,pYY-B23-FLAG-ppBE4 (rAPOBEC1), pYY-B3-SpCas9
                     D10AH840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B4-ScCas9D10A-NLS, pYY-B4-ScCas9 D10A-NLS ,
                     pYY-B5-Sp-ScCas9D10A-NLS, pYY-B5-Sp-ScCas9 D10A-NLS
                     ,pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS
                     ,pYY-B7-SpCas9 D10A-UGI-UGI-NLS,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS ,
                     pYY-B9mutation-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS,pYY-B9-rAPOBEC1-NLS ,
                     pYY-BG34-rAPOBEC1 W90Y R126E-NLS,pYY-BG35 rAPOBEC1 W90Y
                     R126E R132E, pYY-BGX13-hA3A-SpCas9D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl,pYY-BGX27-pmCDA1-SpCas9
                     D10A-UGl, pYY-BGX29-pmCDA5-SpCas9D10A-UGI,
                     pYY-BGX34-rAPOBEC1 W90Y R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BGn-deaminase-NLSCopy, pYY-BX1-pmCDA-SpCas9
                     D10A-UGl,pYY-BX2-TadA-TadA7.10-SpCas9 D10A,
                     pYY-BX3-TadA7.10-SpCas9D10A, pYY-BX4-A3A-SpCas9 D10A-UGl ,
                     pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="To be added in"
     source          <6193..>6225
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /modified_by="hrees"
                     /modified_by="hrees"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS,pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS,pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS,pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS,pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1(1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B16-ScCas9D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS,pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS ,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS,pYY-B19-Flag-TadA-TadA7.10
                     *-NLS , pYY-B2-SpCas9 D10A-NLS,pYY-B2-SpCas9 D10A-NLS ,
                     pYY-B20-flag-rAPOBEC1-NLS,pYY-B20-flag-rAPOBEC1-NLS ,
                     pYY-B21(flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4(rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1)
                     ,pYY-B23-FLAG-ppBE4 (rAPOBEC1), pYY-B3-SpCas9
                     D10AH840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B4-ScCas9D10A-NLS, pYY-B4-ScCas9 D10A-NLS ,
                     pYY-B5-Sp-ScCas9D10A-NLS, pYY-B5-Sp-ScCas9 D10A-NLS
                     ,pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS
                     ,pYY-B7-SpCas9 D10A-UGI-UGI-NLS,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS ,
                     pYY-B9mutation-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS,pYY-B9-rAPOBEC1-NLS ,
                     pYY-BG34-rAPOBEC1 W90Y R126E-NLS,pYY-BG35 rAPOBEC1 W90Y
                     R126E R132E, pYY-BGX13-hA3A-SpCas9D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl,pYY-BGX27-pmCDA1-SpCas9
                     D10A-UGl, pYY-BGX29-pmCDA5-SpCas9D10A-UGI,
                     pYY-BGX34-rAPOBEC1 W90Y R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BGn-deaminase-NLSCopy, pYY-BX1-pmCDA-SpCas9
                     D10A-UGl,pYY-BX2-TadA-TadA7.10-SpCas9 D10A,
                     pYY-BX3-TadA7.10-SpCas9D10A, pYY-BX4-A3A-SpCas9 D10A-UGl ,
                     pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="To be added in"
     Primer          6193..>6225
                     /created_by="hrees"
                     /created_by="hrees"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A),pYY-BGX13-hA3A-SpCas9
                     D10A-UGl, pYY-BGX26-mAID-SpCas9D10A-UGl,
                     pYY-BGX27-pmCDA1-SpCas9 D10A-UGl,pYY-BGX29-pmCDA5-SpCas9
                     D10A-UGI, pYY-BGX34-rAPOBEC1 W90YR126E-UGl,
                     pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX2-TadA-TadA7.10-SpCas9
                     D10A,pYY-BX3-TadA7.10-SpCas9 D10A, pYY-BX4-A3A-SpCas9
                     D10A-UGl, pYY-CMn chimeric APOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="BPNLS"
     Primer          6199..>6247
                     /created_by="hrees"
                     /modified_by="kzhao"
                     /created_by="hrees"
                     /modified_by="kzhao"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A),pYY-BGX13-hA3A-SpCas9
                     D10A-UGl, pYY-BGX26-mAID-SpCas9D10A-UGl,
                     pYY-BGX27-pmCDA1-SpCas9 D10A-UGl,pYY-BGX29-pmCDA5-SpCas9
                     D10A-UGI, pYY-BGX34-rAPOBEC1 W90YR126E-UGl,
                     pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX2-TadA-TadA7.10-SpCas9
                     D10A,pYY-BX3-TadA7.10-SpCas9 D10A, pYY-BX4-A3A-SpCas9
                     D10A-UGl, pYY-CMn chimeric APOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="HR898"
     CDS             6226..6249
                     /created_by="User"
                     /modified_by="User"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A),pYY-BGX13-hA3A-SpCas9
                     D10A-UGl, pYY-BGX26-mAID-SpCas9D10A-UGl,
                     pYY-BGX27-pmCDA1-SpCas9 D10A-UGl,pYY-BGX29-pmCDA5-SpCas9
                     D10A-UGI, pYY-BGX34-rAPOBEC1 W90YR126E-UGl,
                     pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BX1-pmCDA-SpCas9D10A-UGl, pYY-BX2-TadA-TadA7.10-SpCas9
                     D10A,pYY-BX3-TadA7.10-SpCas9 D10A, pYY-BX4-A3A-SpCas9
                     D10A-UGl, pYY-CMn chimeric APOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="SV40 NLS"
     USER            <6250..6260
                     /created_by="nicolegaudelli"
                     /Transferred_From="pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS, pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B16-ScCas9 D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS, pYY-B19-Flag-TadA-TadA7.10*-NLS,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS , pYY-B2-SpCas9 D10A-NLS,
                     pYY-B2-SpCas9 D10A-NLS , pYY-B21
                     (flag-Tad-TadA*7.10-hSpCas9-D10A), pYY-B3-SpCas9 D10A
                     H840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS , pYY-B4-ScCas9
                     D10A-NLS, pYY-B4-ScCas9 D10A-NLS , pYY-B5-Sp-ScCas9
                     D10A-NLS, pYY-B5-Sp-ScCas9 D10A-NLS"
                     /Transferred_Similarity="100.00%"
     misc_feature    complement(6295..6312)
                     /Sequence="TAGAAGGCACAGTCGAGG"
                     /Hairpin_Tm="None"
                     /Tm="55.6"
                     /Self_Dimer_Tm="None"
                     /%GC="55.6"
                     /created_by="primer3"
                     /modified_by="User"
                     /Transferred_From="pYY-AS22 BE4 Y120A"
                     /Transferred_Similarity="100.00%"
                     /label="bgh reverse primer"
                     /note="Geneious type: primer_bind_reverse"
     terminator      complement(6298..6525)
                     /created_by="User"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS,pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS,pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS,pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS,pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1(1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B16-ScCas9D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS,pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS ,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS,pYY-B19-Flag-TadA-TadA7.10
                     *-NLS , pYY-B2-SpCas9 D10A-NLS,pYY-B2-SpCas9 D10A-NLS ,
                     pYY-B20-flag-rAPOBEC1-NLS,pYY-B20-flag-rAPOBEC1-NLS ,
                     pYY-B21(flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4(rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1)
                     ,pYY-B23-FLAG-ppBE4 (rAPOBEC1), pYY-B3-SpCas9
                     D10AH840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B4-ScCas9D10A-NLS, pYY-B4-ScCas9 D10A-NLS ,
                     pYY-B5-Sp-ScCas9D10A-NLS, pYY-B5-Sp-ScCas9 D10A-NLS
                     ,pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS
                     ,pYY-B7-SpCas9 D10A-UGI-UGI-NLS,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS ,
                     pYY-B9mutation-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS,pYY-B9-rAPOBEC1-NLS ,
                     pYY-BG34-rAPOBEC1 W90Y R126E-NLS,pYY-BG35 rAPOBEC1 W90Y
                     R126E R132E, pYY-BGX13-hA3A-SpCas9D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl,pYY-BGX27-pmCDA1-SpCas9
                     D10A-UGl, pYY-BGX29-pmCDA5-SpCas9D10A-UGI,
                     pYY-BGX34-rAPOBEC1 W90Y R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BGn-deaminase-NLSCopy, pYY-BX1-pmCDA-SpCas9
                     D10A-UGl,pYY-BX2-TadA-TadA7.10-SpCas9 D10A,
                     pYY-BX3-TadA7.10-SpCas9D10A, pYY-BX4-A3A-SpCas9 D10A-UGl ,
                     pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="bgH terminator"
     misc_feature    complement(6592..6610)
                     /Sequence="GGAAACAGCTATGACCATG"
                     /Hairpin_Tm="None"
                     /Tm="53.4"
                     /Self_Dimer_Tm="None"
                     /%GC="47.4"
                     /created_by="primer3"
                     /Transferred_From="pYY-AS22 BE4 Y120A"
                     /Transferred_Similarity="100.00%"
                     /label="M13 reverse"
                     /note="Geneious type: primer_bind_reverse"
     promoter        complement(6645..6674)
                     /created_by="User"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS,pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS,pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS,pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS,pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1(1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B16-ScCas9D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS,pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS ,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS,pYY-B19-Flag-TadA-TadA7.10
                     *-NLS , pYY-B2-SpCas9 D10A-NLS,pYY-B2-SpCas9 D10A-NLS ,
                     pYY-B20-flag-rAPOBEC1-NLS,pYY-B20-flag-rAPOBEC1-NLS ,
                     pYY-B21(flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4(rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1)
                     ,pYY-B23-FLAG-ppBE4 (rAPOBEC1), pYY-B3-SpCas9
                     D10AH840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B4-ScCas9D10A-NLS, pYY-B4-ScCas9 D10A-NLS ,
                     pYY-B5-Sp-ScCas9D10A-NLS, pYY-B5-Sp-ScCas9 D10A-NLS
                     ,pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS
                     ,pYY-B7-SpCas9 D10A-UGI-UGI-NLS,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS ,
                     pYY-B9mutation-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS,pYY-B9-rAPOBEC1-NLS ,
                     pYY-BG34-rAPOBEC1 W90Y R126E-NLS,pYY-BG35 rAPOBEC1 W90Y
                     R126E R132E, pYY-BGX13-hA3A-SpCas9D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl,pYY-BGX27-pmCDA1-SpCas9
                     D10A-UGl, pYY-BGX29-pmCDA5-SpCas9D10A-UGI,
                     pYY-BGX34-rAPOBEC1 W90Y R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BGn-deaminase-NLSCopy, pYY-BX1-pmCDA-SpCas9
                     D10A-UGl,pYY-BX2-TadA-TadA7.10-SpCas9 D10A,
                     pYY-BX3-TadA7.10-SpCas9D10A, pYY-BX4-A3A-SpCas9 D10A-UGl ,
                     pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="lac promoter"
     primer_bind     6671..6701
                     /Sequence="TAAAGCCTAGGGTGCCTAATGAGTGAGCTAA"
                     /Hairpin_Tm="35.5"
                     /Tm="66.7"
                     /Self_Dimer_Tm="None"
                     /%GC="45.2"
                     /created_by="primer3"
                     /Transferred_From="pYY-AS22 BE4 Y120A"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-850"
     misc_feature    complement(6790..6800)
                     /Recognition_pattern="(9/12)ACNNNNNCTCC(10/7)"
                     /Transferred_From="pYY-B11-TadA7.10*-NLS,
                     pYY-B11-TadA7.10*-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="BsaXI"
                     /note="Geneious type: restriction site"
     primer_bind     6795..6814
                     /Mismatches=0
                     /%GC="65.0"
                     /Hairpin_Tm="47.9"
                     /Self_Dimer_Tm="None"
                     /Tm="65.2"
                     /Sequence="GGCGGTTTGCGTATTGGGCG"
                     /created_by="primer3"
                     /Transferred_From="pYY-BGn-deaminase-NLS Copy"
                     /Transferred_Similarity="100.00%"
                     /label="Miseq FP"
     rep_origin      6983..7602
                     /created_by="User"
                     /modified_by="User"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS,pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS,pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS,pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS,pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1(1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B16-ScCas9D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS,pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS ,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS,pYY-B19-Flag-TadA-TadA7.10
                     *-NLS , pYY-B2-SpCas9 D10A-NLS,pYY-B2-SpCas9 D10A-NLS ,
                     pYY-B20-flag-rAPOBEC1-NLS,pYY-B20-flag-rAPOBEC1-NLS ,
                     pYY-B21(flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4(rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1)
                     ,pYY-B23-FLAG-ppBE4 (rAPOBEC1), pYY-B3-SpCas9
                     D10AH840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B4-ScCas9D10A-NLS, pYY-B4-ScCas9 D10A-NLS ,
                     pYY-B5-Sp-ScCas9D10A-NLS, pYY-B5-Sp-ScCas9 D10A-NLS
                     ,pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS
                     ,pYY-B7-SpCas9 D10A-UGI-UGI-NLS,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS ,
                     pYY-B9mutation-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS,pYY-B9-rAPOBEC1-NLS ,
                     pYY-BG34-rAPOBEC1 W90Y R126E-NLS,pYY-BG35 rAPOBEC1 W90Y
                     R126E R132E, pYY-BGX13-hA3A-SpCas9D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl,pYY-BGX27-pmCDA1-SpCas9
                     D10A-UGl, pYY-BGX29-pmCDA5-SpCas9D10A-UGI,
                     pYY-BGX34-rAPOBEC1 W90Y R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BGn-deaminase-NLSCopy, pYY-BX1-pmCDA-SpCas9
                     D10A-UGl,pYY-BX2-TadA-TadA7.10-SpCas9 D10A,
                     pYY-BX3-TadA7.10-SpCas9D10A, pYY-BX4-A3A-SpCas9 D10A-UGl ,
                     pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="pBR322"
     misc_feature    7039..7050
                     /Recognition_pattern="GACNNNN^NNGTC"
                     /Transferred_From="pYY-B11-TadA7.10*-NLS,
                     pYY-B11-TadA7.10*-NLS , pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl"
                     /Transferred_Similarity="100.00%"
                     /label="DrdI, AasI, DseDI"
                     /note="Geneious type: restriction site"
     primer_bind     complement(7040..7060)
                     /Mismatches=0
                     /%GC="61.9"
                     /Hairpin_Tm="37.7"
                     /Self_Dimer_Tm="None"
                     /Tm="64.9"
                     /Sequence="CGCCACCTCTGACTTGAGCGT"
                     /created_by="primer3"
                     /Transferred_From="pYY-BGn-deaminase-NLS Copy"
                     /Transferred_Similarity="100.00%"
                     /label="Miseq RP"
     primer_bind     7206..7233
                     /Sequence="AGGTATCTCAGTTCGGTGTAGGTCGTTC"
                     /Hairpin_Tm="None"
                     /Tm="65.5"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /Transferred_From="pYY-AS22 BE4 Y120A"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-851"
     primer_bind     7749..7777
                     /Sequence="TCTGACAGTTACCAATGCTTAATCAGTGA"
                     /Hairpin_Tm="34.3"
                     /Tm="62.7"
                     /Self_Dimer_Tm="8.2"
                     /%GC="37.9"
                     /created_by="primer3"
                     /Transferred_From="pYY-AS22 BE4 Y120A"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-853"
     CDS             complement(7757..8617)
                     /created_by="User"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS,pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS,pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS,pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS,pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1(1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B16-ScCas9D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS,pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS ,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS,pYY-B19-Flag-TadA-TadA7.10
                     *-NLS , pYY-B2-SpCas9 D10A-NLS,pYY-B2-SpCas9 D10A-NLS ,
                     pYY-B20-flag-rAPOBEC1-NLS,pYY-B20-flag-rAPOBEC1-NLS ,
                     pYY-B21(flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4(rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1)
                     ,pYY-B23-FLAG-ppBE4 (rAPOBEC1), pYY-B3-SpCas9
                     D10AH840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B4-ScCas9D10A-NLS, pYY-B4-ScCas9 D10A-NLS ,
                     pYY-B5-Sp-ScCas9D10A-NLS, pYY-B5-Sp-ScCas9 D10A-NLS
                     ,pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS
                     ,pYY-B7-SpCas9 D10A-UGI-UGI-NLS,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS ,
                     pYY-B9mutation-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS,pYY-B9-rAPOBEC1-NLS ,
                     pYY-BG34-rAPOBEC1 W90Y R126E-NLS,pYY-BG35 rAPOBEC1 W90Y
                     R126E R132E, pYY-BGX13-hA3A-SpCas9D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl,pYY-BGX27-pmCDA1-SpCas9
                     D10A-UGl, pYY-BGX29-pmCDA5-SpCas9D10A-UGI,
                     pYY-BGX34-rAPOBEC1 W90Y R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BGn-deaminase-NLSCopy, pYY-BX1-pmCDA-SpCas9
                     D10A-UGl,pYY-BX2-TadA-TadA7.10-SpCas9 D10A,
                     pYY-BX3-TadA7.10-SpCas9D10A, pYY-BX4-A3A-SpCas9 D10A-UGl ,
                     pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="beta-lactamase"
     misc_feature    7825..7835
                     /Recognition_pattern="GACNNN^NNGTC"
                     /Transferred_From="pYY-B11-TadA7.10*-NLS,
                     pYY-B11-TadA7.10*-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="Eam1105I, AhdI, BmeRI, DriI"
                     /note="Geneious type: restriction site"
     primer_bind     8281..8309
                     /Sequence="CCGTAAGATGCTTTTCTGTGACTGGTGAG"
                     /Hairpin_Tm="43.0"
                     /Tm="65.6"
                     /Self_Dimer_Tm="None"
                     /%GC="48.3"
                     /created_by="primer3"
                     /Transferred_From="pYY-AS22 BE4 Y120A"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-854"
     misc_feature    8425..8434
                     /Recognition_pattern="GAANN^NNTTC"
                     /Transferred_From="pYY-B11-TadA7.10*-NLS,
                     pYY-B11-TadA7.10*-NLS"
                     /Transferred_Similarity="100.00%"
                     /label="XmnI, Asp700I, MroXI, PdmI"
                     /note="Geneious type: restriction site"
     promoter        complement(8659..8687)
                     /created_by="User"
                     /modified_by="User"
                     /Transferred_From="pYY-AS22 BE4 Y120A, pYY-ASn BE4
                     alaninescanning, pYY-B1-SpCas9-NLS,
                     pYY-B10-TadA-TadA7.10*-NLS,pYY-B10-TadA-TadA7.10*-NLS ,
                     pYY-B11-TadA7.10*-NLS,pYY-B11-TadA7.10*-NLS ,
                     pYY-B12-pmCDA-NLS,pYY-B13-A3A-NLS Copy,
                     pYY-B14-rAPOBEC1(1-195)-NLS,pYY-B14-rAPOBEC1(1-195)-NLS ,
                     pYY-B15-rAPOBEC1(1-211)-NLS, pYY-B15-rAPOBEC1 (1-211)-NLS
                     , pYY-B16-ScCas9D10A R211K, N404K-NLS, pYY-B17 SaCas9
                     D10A-NLS,pYY-B18-SaCas9 D10A-UGI-UGI-NLS,
                     pYY-B18-SaCas9D10A-UGI-UGI-NLS ,
                     pYY-B19-Flag-TadA-TadA7.10*-NLS,pYY-B19-Flag-TadA-TadA7.10
                     *-NLS , pYY-B2-SpCas9 D10A-NLS,pYY-B2-SpCas9 D10A-NLS ,
                     pYY-B20-flag-rAPOBEC1-NLS,pYY-B20-flag-rAPOBEC1-NLS ,
                     pYY-B21(flag-Tad-TadA*7.10-hSpCas9-D10A),
                     pYY-B22-FLAG-BE4(rAPOBEC1), pYY-B22-FLAG-BE4 (rAPOBEC1)
                     ,pYY-B23-FLAG-ppBE4 (rAPOBEC1), pYY-B3-SpCas9
                     D10AH840A-NLS, pYY-B3-SpCas9 D10A H840A-NLS ,
                     pYY-B4-ScCas9D10A-NLS, pYY-B4-ScCas9 D10A-NLS ,
                     pYY-B5-Sp-ScCas9D10A-NLS, pYY-B5-Sp-ScCas9 D10A-NLS
                     ,pYY-B6-SpCas9-UGI-UGI-NLS, pYY-B6-SpCas9-UGI-UGI-NLS
                     ,pYY-B7-SpCas9 D10A-UGI-UGI-NLS,
                     pYY-B7-SpCas9D10A-UGI-UGI-NLS , pYY-B8-SpCas9 D10A
                     H840-UGI-UGI-NLS,pYY-B8-SpCas9 D10A H840-UGI-UGI-NLS ,
                     pYY-B9mutation-rAPOBEC1-NLS,
                     pYY-B9-rAPOBEC1-NLS,pYY-B9-rAPOBEC1-NLS ,
                     pYY-BG34-rAPOBEC1 W90Y R126E-NLS,pYY-BG35 rAPOBEC1 W90Y
                     R126E R132E, pYY-BGX13-hA3A-SpCas9D10A-UGl,
                     pYY-BGX26-mAID-SpCas9 D10A-UGl,pYY-BGX27-pmCDA1-SpCas9
                     D10A-UGl, pYY-BGX29-pmCDA5-SpCas9D10A-UGI,
                     pYY-BGX34-rAPOBEC1 W90Y R126E-UGl,pYY-BGX4-hAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX5-ppAPOBEC1-SpCas9 D10A-UGl
                     ,pYY-BGX6-ocAPOBEC1-SpCas9
                     D10A-UGl,pYY-BGX8-mAPOBEC2-SpCas9 D10A-UGl
                     ,pYY-BGXn-deaminase-SpCas9 D10A-UGl
                     ,pYY-BGY1-rAPOBEC1-SpCas9 D10A-UGl,
                     pYY-BGn-deaminase-NLSCopy, pYY-BX1-pmCDA-SpCas9
                     D10A-UGl,pYY-BX2-TadA-TadA7.10-SpCas9 D10A,
                     pYY-BX3-TadA7.10-SpCas9D10A, pYY-BX4-A3A-SpCas9 D10A-UGl ,
                     pYY-CMn chimericAPOBECs"
                     /Transferred_Similarity="100.00%"
                     /label="pAmpR"
     primer_bind     8738..8766
                     /Sequence="AGTGCCACCTGACGTCGACGGATCGGGAG"
                     /Hairpin_Tm="48.7"
                     /Tm="74.0"
                     /Self_Dimer_Tm="33.5"
                     /%GC="65.5"
                     /created_by="primer3"
                     /Transferred_From="pYY-AS22 BE4 Y120A"
                     /Transferred_Similarity="100.00%"
                     /label="NMG-838"
     misc_feature    8792^8793
                     /Original_Bases="T"
                     /label="T"
                     /note="Geneious type: Editing History Deletion"
ORIGIN      
        1 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
       61 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      121 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      181 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      241 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      301 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      361 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      421 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      481 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      541 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      601 tctatataag cagagctggt ttagtgaacc gtcagatccg ctagagatcc gcggccgcta
      661 atacgactca ctatagggag agccgccacc atgacctctg agaagggccc tagcacaggc
      721 gaccccaccc tgcggcggag aatcgagagc tgggagttcg acgtgttcta cgaccctaga
      781 gaactggcca aggaaacctg cctgctgtac gagatcaagt ggggcatgag cagaaagatc
      841 tggcggagct ctggcaagaa caccaccaac cacgtggaag tgaatttcat caagaagttc
      901 accagcgaga gaaggttcca cagcagcatc agctgcagca tcacctggtt cctgagctgg
      961 tccccttgct gggaatgcag ccaggccatc agagagttcc tgagccaaca ccccggagtg
     1021 acactggtga tctacgtggc cagactgttc tggcacatgg accagagaaa cagacagggc
     1081 ctgagagatc tggtcaacag cggcgtgact atccagatca tgcgggccag cgagtactac
     1141 cactgttggc ggaacttcgt gaactacccc cccggcgatg aggcccactg gcctcagtac
     1201 cctcctctgt ggatgatgct gtacgccctg gaactgcact gcatcatcct gtctctgcct
     1261 ccatgtctga agatctctag aagatggcag aaccacctgg ccttcttcag actgcacctg
     1321 cagaattgcc actaccagac catccccccc cacatcctgc tggctacagg cctgatccac
     1381 ccttctgtga cctggagact taagagcgga ggatctagcg gcggctctag cggatctgag
     1441 acacctggca caagcgagtc tgccacacct gagagtagcg gcggatcttc tggtggctct
     1501 gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc
     1561 accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac
     1621 agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc
     1681 acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat
     1741 ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg
     1801 gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac
     1861 atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa
     1921 ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg
     1981 atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg
     2041 gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc
     2101 aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg
     2161 ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg
     2221 attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat
     2281 gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag
     2341 atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg
     2401 ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg
     2461 atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag
     2521 cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc
     2581 tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa
     2641 aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag
     2701 cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc
     2761 attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag
     2821 aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga
     2881 ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg
     2941 gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac
     3001 ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat
     3061 aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc
     3121 ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg
     3181 aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc
     3241 ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc
     3301 aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg
     3361 accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac
     3421 ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg
     3481 ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat
     3541 ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc
     3601 ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac
     3661 gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg
     3721 aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc
     3781 gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg
     3841 aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg
     3901 gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat
     3961 atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc
     4021 gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac
     4081 aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac
     4141 tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc
     4201 aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg
     4261 gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact
     4321 aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag
     4381 ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac
     4441 caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac
     4501 cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg
     4561 atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac
     4621 atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct
     4681 ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc
     4741 accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag
     4801 acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc
     4861 agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat
     4921 tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa
     4981 gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt
     5041 ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac
     5101 tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag
     5161 aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac
     5221 tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag
     5281 cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc
     5341 ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc
     5401 atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct
     5461 gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag
     5521 gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac
     5581 ctgtctcagc tgggaggtga ctctggtgga agcggaggat ctggcggcag caccaatctg
     5641 agcgacatca tcgagaaaga gacaggcaag cagctggtca tccaagagtc catcctgatg
     5701 ctgcctgaag aggtggaaga agtgatcggc aacaagcccg agtccgacat cctggtgcac
     5761 accgcctacg atgagagcac cgacgagaac gtgatgctgc tgacctctga cgcccctgag
     5821 tacaagcctt gggctctcgt gatccaggac agcaacggcg agaacaagat caagatgctg
     5881 agcggcggct ctggtggctc tggcggatct acaaacctgt ccgatattat tgagaaagaa
     5941 accgggaaac agctcgtgat tcaagagtct attctcatgc tcccggaaga agtcgaggaa
     6001 gtcattggaa acaagcctga gagcgatatt ctggtccata cagcctacga cgagtctacc
     6061 gatgagaatg tcatgctcct caccagcgac gctcccgagt ataagccatg ggcacttgtc
     6121 attcaggact ccaatgggga aaacaaaatc aaaatgctcc caaagaaaaa acgcaaggtg
     6181 gagggagctg ataagcgcac cgccgatggt tccgagttcg aaagccccaa gaagaagagg
     6241 aaagtctaac cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga
     6301 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     6361 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     6421 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     6481 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     6541 gaaccagctg gggctcgata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     6601 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6661 gcataaagtg taaagcctag ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6721 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     6781 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6841 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6901 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6961 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7021 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7081 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7141 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7201 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7261 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7321 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7381 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     7441 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7501 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7561 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7621 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7681 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7741 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7801 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7861 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7921 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7981 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8041 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8101 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8161 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8221 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8281 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8341 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8401 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8461 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8521 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8581 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8641 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     8701 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     8761 cgggagatcg atctcccgat cccctagggt ctactctcag tacaatctgc tctgatgccg
     8821 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     8881 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
     8941 gggttaggcg ttttgcgc
//
