LOCUS       268-pYY-BGX5-ppAPOBEC1-SpCas9_D10A-UGl_        8958 bp    DNA     circular UNA 22-DEC-2019
DEFINITION  .
ACCESSION   urn.local...cs-7ud4snq
VERSION     urn.local...cs-7ud4snq
KEYWORDS    .
SOURCE      .
  ORGANISM  .
FEATURES             Location/Qualifiers
     promoter        35..611
                     /created_by="User"
                     /modified_by="User"
                     /label="pCMV immearly protomter"
     misc_feature    114..282
                     /created_by="User"
                     /label="CAG enhancer"
     misc_feature    239
                     /Original_Bases="C"
                     /label="C"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     294..326
                     /Sequence="ACGCCCCCTATTGACGTCAATGACGGTAAATGG"
                     /Hairpin_Tm="56.5"
                     /Tm="71.1"
                     /Self_Dimer_Tm="30.9"
                     /%GC="51.5"
                     /created_by="primer3"
                     /label="NMG-839"
     promoter        569..638
                     /created_by="User"
                     /label="CMV promoter"
     unsure          645..>658
                     /created_by="User"
                     /label="USER"
     primer_bind     651..677
                     /Sequence="GCGGCCGCTAATACGACTCACTATAGG"
                     /Hairpin_Tm="38.3"
                     /Tm="66.5"
                     /Self_Dimer_Tm="41.7"
                     /%GC="55.6"
                     /modified_by="yiyu"
                     /label="21 NotI pmCDA FP"
     unsure          659
                     /created_by="User"
                     /label="USER"
     promoter        659..677
                     /created_by="User"
                     /label="T7 protomet"
     primer_bind     659..678
                     /Sequence="TAATACGACTCACTATAGGG"
                     /Hairpin_Tm="None"
                     /Tm="50.3"
                     /Self_Dimer_Tm="None"
                     /%GC="40.0"
                     /created_by="primer3"
                     /label="T7"
     promoter        691..1398
                     /created_by="yiyu"
                     /label="ppAPOBEC1"
     primer_bind     1399..1404
                     /Sequence="CTTAAG"
                     /created_by="yiyu"
                     /label="LK"
     CDS             1405..1500
                     /created_by="ngaudelli"
                     /label="linker"
     misc_feature    complement(1479..1498)
                     /Sequence="AGCCACCAGAAGATCCGCCG"
                     /Hairpin_Tm="None"
                     /Tm="64.7"
                     /Self_Dimer_Tm="None"
                     /%GC="65.0"
                     /created_by="primer3"
                     /modified_by="ngaudelli"
                     /label="NMG-B140"
                     /note="Geneious type: primer_bind_reverse"
     unsure          1485..1498
                     /created_by="ngaudelli"
                     /label="USER"
     primer_bind     1485..1521
                     /Sequence="ATCTTCTGGTGGCTCTGACAAGAAGTACAGCATCGGC"
                     /created_by="nicolegaudelli"
                     /label="NMG-B235"
     unsure          1501..6159
                     /created_by="angelicamessana"
                     /label="Cas9"
     CDS             1501..5601
                     /note="hSpCas9"
                     /modified_by="jonlevy"
                     /label="fz hSpCas9"
     overhang        1502..1517
                     /created_by="kzhao"
                     /label="USER"
     misc_feature    1503..6158
                     /Original_Bases="TCTGGTGGAAGCGGAGGATCTGGCGGCAG"
                     /label="TCTGGTGGAAGCGGAGGATCTGGCGGCAG"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1791..1801
                     /Recognition_pattern="CCANNNN^NTGG"
                     /annotation_group="CCANNNNNTGG"
                     /label="PflMI"
                     /note="Geneious type: restriction site"
     primer_bind     2626..2645
                     /Sequence="AAGCCCATCCTGGAAAAGAT"
                     /Transferred_From="LCv2_seq12"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq12"
     primer_bind     3125..3143
                     /Sequence="AGCAGAAAAAGGCCATCGT"
                     /Transferred_From="LCv2_seq13"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq13"
     primer_bind     complement(3186..3236)
                     /Sequence="ATTTCCACGGAGTCGAAGCACTCGATTTTCTTGAAGTAGTCCTCTTT
                     CAGC"
                     /%GC="45.1"
                     /Transferred_From="876_fzCas9-resplice"
                     /Transferred_Similarity="100.00%"
                     /label="876_fzCas9-resplice"
     primer_bind     complement(3186..3216)
                     /Sequence="CTCGATTTTCTTGAAGTAGTCCTCTTTCAGC"
                     /%GC="41.9"
                     /Transferred_From="901_fzCas9_NpuC-rev"
                     /Transferred_Similarity="100.00%"
                     /label="901_fzCas9_NpuC-rev"
     primer_bind     3217..3239
                     /Sequence="TGCTTCGACTCCGTGGAAATCTC"
                     /%GC="52.2"
                     /Transferred_From="841_FZ_Cas9-C11-fwd"
                     /Transferred_Similarity="100.00%"
                     /label="841_FZ_Cas9-C11-fwd"
     primer_bind     3619..3638
                     /Sequence="ATCCAGAAAGCCCAGGTGTC"
                     /Transferred_From="LCv2_seq14"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq14"
     misc_feature    3972..3982
                     /Recognition_pattern="CCANNNN^NTGG"
                     /annotation_group="CCANNNNNTGG"
                     /label="PflMI"
                     /note="Geneious type: restriction site"
     primer_bind     4112..4131
                     /Sequence="CCGAAGAGGTCGTGAAGAAG"
                     /Transferred_From="LCv2_seq15"
                     /Transferred_Similarity="100.00%"
                     /label="LCv2_seq15"
     unsure          <5602..>6159
                     /created_by="ngaudelli"
                     /label="gene block"
     CDS             5602..6159
                     /label="ORF1"
     primer_bind     5602..5620
                     /Sequence="TCTGGTGGAAGCGGAGGAT"
                     /Hairpin_Tm="46.3"
                     /Tm="60.3"
                     /Self_Dimer_Tm="None"
                     /%GC="57.9"
                     /modified_by="yiyu"
                     /label="10 pCMV BB UGI FP"
     misc_feature    complement(6128..6158)
                     /Sequence="AGCATTTTGATTTTGTTTTCCCCATTGGAGT"
                     /Hairpin_Tm="46.2"
                     /Tm="64.9"
                     /Self_Dimer_Tm="3.6"
                     /%GC="35.5"
                     /created_by="primer3"
                     /label="NMG-B144"
                     /note="Geneious type: primer_bind_reverse"
     Junction        6158
                     /Reads_using=5
                     /Reads_supporting_discovery=2
                     /Junction_Type="Deletion"
                     /Deletion_Size=30
                     /Junction_Source="6095+"
                     /Junction_Destination="6126+"
                     /ID="junction 1"
                     /label="junction 1"
     CDS             <6160..6250
                     /created_by="nicolegaudelli"
                     /label="NLS tag"
     USER            6167..6179
                     /modified_by="ngaudelli"
     primer_bind     6167..6213
                     /Sequence="AAAAACGCAAGGTGGAGGGAGCTGATAAGCGCACCGCCGATGGTTCC
                     "
                     /created_by="ngaudelli"
                     /label="NMG-B223"
     source          <6181..>6192
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /modified_by="hrees"
                     /label="To be added in"
     Primer          6193..>6225
                     /created_by="hrees"
                     /label="BPNLS"
     source          <6193..>6225
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /modified_by="hrees"
                     /label="To be added in"
     Primer          6199..>6247
                     /created_by="hrees"
                     /modified_by="kzhao"
                     /label="HR898"
     overhang        6199..6207
                     /created_by="kzhao"
                     /label="USER"
     CDS             6226..6249
                     /created_by="User"
                     /modified_by="User"
                     /label="SV40 NLS"
     misc_feature    complement(6295..6312)
                     /Sequence="TAGAAGGCACAGTCGAGG"
                     /Hairpin_Tm="None"
                     /Tm="55.6"
                     /Self_Dimer_Tm="None"
                     /%GC="55.6"
                     /created_by="primer3"
                     /modified_by="User"
                     /label="bgh reverse primer"
                     /note="Geneious type: primer_bind_reverse"
     terminator      complement(6298..6525)
                     /created_by="User"
                     /label="bgH terminator"
     misc_feature    complement(6592..6610)
                     /Sequence="GGAAACAGCTATGACCATG"
                     /Hairpin_Tm="None"
                     /Tm="53.4"
                     /Self_Dimer_Tm="None"
                     /%GC="47.4"
                     /created_by="primer3"
                     /label="M13 reverse"
                     /note="Geneious type: primer_bind_reverse"
     promoter        complement(6645..6674)
                     /created_by="User"
                     /label="lac promoter"
     primer_bind     6671..6701
                     /Sequence="TAAAGCCTAGGGTGCCTAATGAGTGAGCTAA"
                     /Hairpin_Tm="35.5"
                     /Tm="66.7"
                     /Self_Dimer_Tm="None"
                     /%GC="45.2"
                     /created_by="primer3"
                     /label="NMG-850"
     rep_origin      6983..7602
                     /created_by="User"
                     /modified_by="User"
                     /label="pBR322"
     misc_feature    7194
                     /Original_Bases
                     /label
                     /note="Geneious type: Editing History Insertion"
     primer_bind     7206..7233
                     /Sequence="AGGTATCTCAGTTCGGTGTAGGTCGTTC"
                     /Hairpin_Tm="None"
                     /Tm="65.5"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label="NMG-851"
     misc_feature    7439
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     primer_bind     7749..7777
                     /Sequence="TCTGACAGTTACCAATGCTTAATCAGTGA"
                     /Hairpin_Tm="34.3"
                     /Tm="62.7"
                     /Self_Dimer_Tm="8.2"
                     /%GC="37.9"
                     /created_by="primer3"
                     /label="NMG-853"
     CDS             complement(7757..8617)
                     /created_by="User"
                     /label="beta-lactamase"
     primer_bind     8281..8309
                     /Sequence="CCGTAAGATGCTTTTCTGTGACTGGTGAG"
                     /Hairpin_Tm="43.0"
                     /Tm="65.6"
                     /Self_Dimer_Tm="None"
                     /%GC="48.3"
                     /created_by="primer3"
                     /label="NMG-854"
     promoter        complement(8659..8687)
                     /created_by="User"
                     /modified_by="User"
                     /label="pAmpR"
     primer_bind     8738..8766
                     /Sequence="AGTGCCACCTGACGTCGACGGATCGGGAG"
                     /Hairpin_Tm="48.7"
                     /Tm="74.0"
                     /Self_Dimer_Tm="33.5"
                     /%GC="65.5"
                     /created_by="primer3"
                     /label="NMG-838"
     misc_feature    8792
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
ORIGIN      
        1 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
       61 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      121 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      181 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      241 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      301 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      361 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      421 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      481 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      541 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      601 tctatataag cagagctggt ttagtgaacc gtcagatccg ctagagatcc gcggccgcta
      661 atacgactca ctatagggag agccgccacc atgacctctg agaagggccc tagcacaggc
      721 gaccccaccc tgcggcggag aatcgagagc tgggagttcg acgtgttcta cgaccctaga
      781 gaactgagaa aggaaacctg cctgctgtac gagatcaagt ggggcatgag cagaaagatc
      841 tggcggagct ctggcaagaa caccaccaac cacgtggaag tgaatttcat caagaagttc
      901 accagcgaga gaaggttcca cagcagcatc agctgcagca tcacctggtt cctgagctgg
      961 tccccttgct gggaatgcag ccaggccatc agagagttcc tgagccaaca ccccggagtg
     1021 acactggtga tctacgtggc cagactgttc tggcacatgg accagagaaa cagacagggc
     1081 ctgagagatc tggtcaacag cggcgtgact atccagatca tgcgggccag cgagtactac
     1141 cactgttggc ggaacttcgt gaactacccc cccggcgatg aggcccactg gcctcagtac
     1201 cctcctctgt ggatgatgct gtacgccctg gaactgcact gcatcatcct gtctctgcct
     1261 ccatgtctga agatctctag aagatggcag aaccacctgg ccttcttcag actgcacctg
     1321 cagaattgcc actaccagac catccccccc cacatcctgc tggctacagg cctgatccac
     1381 ccttctgtga cctggagact taagagcgga ggatctagcg gcggctctag cggatctgag
     1441 acacctggca caagcgagtc tgccacacct gagagtagcg gcggatcttc tggtggctct
     1501 gacaagaagt acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc
     1561 accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac
     1621 agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc
     1681 acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat
     1741 ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg
     1801 gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac
     1861 atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa
     1921 ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg
     1981 atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg
     2041 gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc
     2101 aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg
     2161 ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg
     2221 attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat
     2281 gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag
     2341 atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg
     2401 ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg
     2461 atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag
     2521 cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc
     2581 tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa
     2641 aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag
     2701 cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc
     2761 attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag
     2821 aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga
     2881 ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg
     2941 gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac
     3001 ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat
     3061 aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc
     3121 ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg
     3181 aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc
     3241 ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc
     3301 aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg
     3361 accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac
     3421 ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg
     3481 ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat
     3541 ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc
     3601 ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac
     3661 gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg
     3721 aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc
     3781 gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg
     3841 aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg
     3901 gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat
     3961 atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc
     4021 gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac
     4081 aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac
     4141 tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc
     4201 aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg
     4261 gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact
     4321 aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag
     4381 ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac
     4441 caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac
     4501 cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg
     4561 atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac
     4621 atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct
     4681 ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc
     4741 accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag
     4801 acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc
     4861 agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat
     4921 tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa
     4981 gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt
     5041 ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac
     5101 tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag
     5161 aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac
     5221 tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag
     5281 cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc
     5341 ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc
     5401 atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct
     5461 gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag
     5521 gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac
     5581 ctgtctcagc tgggaggtga ctctggtgga agcggaggat ctggcggcag caccaatctg
     5641 agcgacatca tcgagaaaga gacaggcaag cagctggtca tccaagagtc catcctgatg
     5701 ctgcctgaag aggtggaaga agtgatcggc aacaagcccg agtccgacat cctggtgcac
     5761 accgcctacg atgagagcac cgacgagaac gtgatgctgc tgacctctga cgcccctgag
     5821 tacaagcctt gggctctcgt gatccaggac agcaacggcg agaacaagat caagatgctg
     5881 agcggcggct ctggtggctc tggcggatct acaaacctgt ccgatattat tgagaaagaa
     5941 accgggaaac agctcgtgat tcaagagtct attctcatgc tcccggaaga agtcgaggaa
     6001 gtcattggaa acaagcctga gagcgatatt ctggtccata cagcctacga cgagtctacc
     6061 gatgagaatg tcatgctcct caccagcgac gctcccgagt ataagccatg ggcacttgtc
     6121 attcaggact ccaatgggga aaacaaaatc aaaatgctcc caaagaaaaa acgcaaggtg
     6181 gagggagctg ataagcgcac cgccgatggt tccgagttcg aaagccccaa gaagaagagg
     6241 aaagtctaac cggtcatcat caccatcacc attgagttta aacccgctga tcagcctcga
     6301 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     6361 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     6421 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     6481 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     6541 gaaccagctg gggctcgata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     6601 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6661 gcataaagtg taaagcctag ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6721 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     6781 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6841 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6901 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6961 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7021 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7081 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7141 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     7201 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     7261 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     7321 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7381 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     7441 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7501 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7561 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7621 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7681 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7741 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7801 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7861 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7921 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7981 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8041 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8101 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     8161 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     8221 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     8281 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     8341 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     8401 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     8461 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8521 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8581 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8641 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     8701 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     8761 cgggagatcg atctcccgat cccctagggt ctactctcag tacaatctgc tctgatgccg
     8821 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     8881 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
     8941 gggttaggcg ttttgcgc
//
