LOCUS New_DNA 3634 bp ds-DNA circular 05-JAN-2016 DEFINITION . ACCESSION VERSION SOURCE . ORGANISM . COMMENT Insert from New DNA 13 to 1024 COMMENT Backbone from /Users/samirsissaoui/Documents/APE/213 pDONRP4-P1r.ape 2830 to 674 COMMENT COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_recomb 1687..1700 /label=attB1 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 668..674 /label=attB4 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 1687..1836 /label=AttP1 /ApEinfo_fwdcolor=plum /ApEinfo_revcolor=plum /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 1687..1700 /label=AttL1 /ApEinfo_fwdcolor=plum /ApEinfo_revcolor=plum /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 593..674 /label=attP4 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 668..674 /label=attR4 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 1687..1836 /label=AttR1 /ApEinfo_fwdcolor=plum /ApEinfo_revcolor=plum /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_feature 1687..1693 /label=Gateway recomb1 /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 misc_recomb 593..674 /label=attL4 /ApEinfo_fwdcolor=#dda0dd /ApEinfo_revcolor=#dda0dd /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} width 5 offset 0 ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCGC GTTAACGCTA CCATGGAGCT CCAAATAATG 601 ATTTTATTTT GACTGATAGT GACCTGTTCG TTGCAACAAA TTGATAAGCA ATGCTTTTTT 661 ATAATGCCAA CTTTgtatag aaaagttgGG CTGCTGATTC ATATCCCAGT GAGGTCATCT 721 GAATTTTGGG GGACCTCAAA TAGAAATCAT ATGGGGGAAA GAATGAGCCT AGCTCAAACC 781 AGACAGGCTG AGAGCTTATC TTGCCAGGAC ATAGGCTCCC CGGTGGGTGG GGGAGATGGC 841 CATTGCTGCC TTTCTTTCCT TCCCCACTGA ATTTAAGCAG ATGCAGTTAG TCACTGTGCA 901 AAGGAGTATG TGCAAATCTA AGTATTCATG TTTATGTGTG GTTGTCAGGT CTAAATCAGC 961 CAGGCAGTAA TTTCTCTGGG AGCCCAATGT AGGCACTAAA TTTTGCACTC AACCACCGAA 1021 TGGTCCACAT GCCCCTTAAT CTGTCTTCCT GCAGGAGGGC TCCCTTGGCA CCATCCTGCC 1081 TGAACTTGGG GCCAGCCTTT CACTGGAGCC CCTTCCCGTG AATTATGGCC TACAGCATGG 1141 GGGCTGAATA GAAAAAAAAA AACCCTCCAG AAATTCAACT GGAAGGGCTG ACCGGAATTC 1201 AACCTAATGT TGAGAATAGC AGGAAAGCTT CTATTATTCT ACTTCATTCT ATCTGCTATG 1261 AGGCAGTGCG GTCGCCTGGA GTCGACTGAG CTTAACTCAT TTCCCTGGAT TTCCGAGTCA 1321 CAGCCATGGT GCCTGCTAtA GttAAcgATC gaACTCCTGA GACTCACTAC ACTTCCTGAT 1381 AGGAcGtACT caCGTacCgC CTAGTaACaT cAtACgtTcc TAtAGGAGTC ATAATATGAC 1441 CTAGACAAGA CCTAGGAGCC AATCAGAATG CAAATGATTG ATGGGAGTGC TTTTGGTTAT 1501 CTACTGTTTG TCCTGACGCT TGGCGTGGTT CTTTGTATTA TGATTGTGTG GGCAGTAGGC 1561 TTAAAAATAC ATACAGGCAT TGCTTTCCAC ATTGTTGAAG GTTAATCCTA AGGTGTGGCA 1621 AAGAAACTAT ATTTTATAGA CTGGAACCTC ATGGGCACCA GCTAGTTTTT GCTCTTCCTc 1681 aagtttGTAC AAAAAAGTTG AACGAGAAAC GTAAAATGAT ATAAATATCA ATATATTAAA 1741 TTAGATTTTG CATAAAAAAC AGACTACATA ATACTGTAAA ACACAACATA TGCAGTCACT 1801 ATGAATCAAC TACTTAGATG GTATTAGTGA CCTGTAGAAT TCGAGCTCTA GAGCTGCAGG 1861 GCGGCCGCGA TATCCCCTAT AGTGAGTCGT ATTACATGGT CATAGCTGTT TCCTGGCAGC 1921 TCTGGCCCGT GTCTCAAAAT CTCTGATGTT ACATTGCACA AGATAAAAAT ATATCATCAT 1981 GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG GGGTGTTATG AGCCATATTC 2041 AACGGGAAAC GTCGAGGCCG CGATTAAATT CCAACATGGA TGCTGATTTA TATGGGTATA 2101 AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT CTATCGCTTG TATGGGAAGC 2161 CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG CGTTGCCAAT GATGTTACAG 2221 ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC TCTTCCGACC ATCAAGCATT 2281 TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC GATCCCCGGA AAAACAGCAT 2341 TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT TGTTGATGCG CTGGCAGTGT 2401 TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC TTTTAACAGC GATCGCGTAT 2461 TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT GGTTGATGCG AGTGATTTTG 2521 ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA AGAAATGCAT AAACTTTTGC 2581 CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC ACTTGATAAC CTTATTTTTG 2641 ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT CGGAATCGCA GACCGATACC 2701 AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC TCCTTCATTA CAGAAACGGC 2761 TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA ATTGCAGTTT CATTTGATGC 2821 TCGATGAGTT TTTCTAATCA GAATTGGTTA ATTGGTTGTA ACACTGGCAG AGCATTACGC 2881 TGACTTGACG GGACGGCGCA AGCTCATGAC CAAAATCCCT TAACGTGAGT TACGCGTCGT 2941 TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC 3001 TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC 3061 CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC 3121 CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC 3181 CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT 3241 CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT 3301 GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT 3361 ACCTACAGCG TGAGCATTGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT 3421 ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG 3481 CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT 3541 GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT 3601 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTT //