LOCUS       Exported               15815 bp ds-DNA     circular SYN 16-DEC-2019
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15815)
  AUTHORS   Seth Darst
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Dec 24, 2019 from SnapGene 4.3.10
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15815
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             89..1078
                     /label=rpoA
                     /note="rpoA"
     gene            1169..5197
                     /label=rpoB
     CDS             5274..9566
                     /codon_start=1
                     /label=rpoC
                     /translation="VKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETIN
                     YRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERMGH
                     IELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQILTEE
                     QYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLTKRIK
                     LLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNNRLKR
                     LLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGKQGRFRQ
                     NLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLATTIKAAKK
                     MVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCAAYN
                     ADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVLGLYYMTRDC
                     VNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAKTSLKDTTVGR
                     AILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQIMYTGFAYAARS
                     GASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKVIDIWAAANDRVS
                     KAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQLAGMRGLMAKPDGS
                     IIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDLVVTED
                     DCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADILVPRNTLLHEQWCDL
                     LEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEAIGVIAAQSIGEPGTQL
                     TMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGKLVITSRNTELKLIDEFG
                     RTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITEVSGFVRFTDMIDGQTITR
                     QTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDVLIPGTDMPAQYFLPGKAIV
                     QLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFEARRPKEPAILAEISGIVSFG
                     KETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERVERGDVISDGPEAPHDILRLRG
                     VHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLRKATIVNAGSSDFLEGEQVEYSR
                     VKIANRELEANGKVGATYSRDLLGITKASLATESFISAASFQETTRVLTEAAVAGKRDE
                     LRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEAPAAPQVTAEDASASLAELLNAGLG
                     GSDNELELEVLFQGPSSGHHHHHHHHHH"
     misc_feature    5703..5810
                     /label=Clamp toe
     CDS             9501..9533
                     /label=ppx
                     /note="ppx"
     CDS             9501..9524
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             9534..9566
                     /label=His10
     CDS             9534..9563
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             10387..10398
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             10508..10663
                     /codon_start=1
                     /gene="Bacillus circulans chiA"
                     /product="chitin binding domain from chitinase A1 (Watanabe
                     et al., 1994)"
                     /label=CBD
                     /translation="TTNPGVSAWQVNTAYTAGQLVTYNGKTYKCLQPHTSLAGWEPSNV
                     PALWQLQ"
     CDS             10694..10969
                     /label=rpoZ
     promoter        11011..11115
                     /gene="bla"
                     /label=AmpR promoter
     CDS             11116..11976
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      complement(12018..12531)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      12642..13230
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(13599..13790)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(14347..15429)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(15430..15507)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atatacatat gcagggttct gtgacagagt ttctaaaacc
      121 gcgcctggtt gatatcgagc aagtgagttc gacgcacgcc aaggtgaccc ttgagccttt
      181 agagcgtggc tttggccata ctctgggtaa cgcactgcgc cgtattctgc tctcatcgat
      241 gccgggttgc gcggtgaccg aggttgagat tgatggtgta ctacatgagt acagcaccaa
      301 agaaggcgtt caggaagata tcctggaaat cctgctcaac ctgaaagggc tggcggtgag
      361 agttcagggc aaagatgaag ttattcttac cttgaataaa tctggcattg gccctgtgac
      421 tgcagccgat atcacccacg acggtgatgt cgaaatcgtc aagccgcagc acgtgatctg
      481 ccacctgacc gatgagaacg cgtctattag catgcgtatc aaagttcagc gcggtcgtgg
      541 ttatgtgccg gcttctaccc gaattcattc ggaagaagat gagcgcccaa tcggccgtct
      601 gctggtcgac gcatgctaca gccctgtgga gcgtattgcc tacaatgttg aagcagcgcg
      661 tgtagaacag cgtaccgacc tggacaagct ggtcatcgaa atggaaacca acggcacaat
      721 cgatcctgaa gaggcgattc gtcgtgcggc aaccattctg gctgaacaac tggaggcttt
      781 cgttgactta cgtgatgtac gtcagcctga agtgaaagaa gagaaaccag agttcgatcc
      841 gatcctgctg cgccctgttg acgatctgga attgactgtc cgctctgcta actgccttaa
      901 agcagaagct atccactata tcggtgatct ggtacagcgt accgaggttg agctccttaa
      961 aacgcctaac cttggtaaaa aatctcttac tgagattaaa gacgtgctgg cttcccgtgg
     1021 actgtctctg ggcatgcgcc tggaaaactg gccaccggca agcatcgctg acgagtaacc
     1081 ggatccccga tccgtggact tgtcagcgac tgaggaaaca gaccatggat ccccgatccg
     1141 tcgacttgtc agcgagctga ggaaccctat ggtttactcc tataccgaga aaaaacgtat
     1201 tcgtaaggat tttggtaaac gtccacaagt tctggatgta ccttatctcc tttctatcca
     1261 gcttgactcg tttcagaaat ttatcgagca agatcctgaa gggcagtatg gtctggaagc
     1321 tgctttccgt tccgtattcc cgattcagag ctacagcggt aattccgagc tgcaatacgt
     1381 cagctaccgc cttggcgaac cggtgtttga cgtccaggaa tgtcaaatcc gtggcgtgac
     1441 ctattccgca ccgctgcgcg ttaaactgcg tctggtgatc tatgagcgcg aagcgccgga
     1501 aggcaccgta aaagacatta aagaacaaga agtctacatg ggcgaaattc cgctcatgac
     1561 agacaacggt acctttgtta tcaacggtac tgagcgtgtt atcgtttccc agctgcaccg
     1621 tagtccgggc gtcttctttg actccgacaa aggtaaaacc cactcttcgg gtaaagtgct
     1681 gtataacgcg cgtatcatcc cttaccgtgg ttcctggctg gacttcgaat tcgatccgaa
     1741 ggacaacctg ttcgtacgta tcgaccgtcg ccgtaaactg cctgcgacca tcattctgcg
     1801 cgccctgaac tacaccacag agcagatcct cgacctgttc tttgaaaaag ttatctttga
     1861 aatccgtgat aacaagctgc agatggaact ggtgccggaa cgcctgcgtg gtgaaaccgc
     1921 atcttttgac atcgaagcta acggtaaagt gtacgtagaa aaaggccgcc gtatcactgc
     1981 gcgccacatt cgccagctgg aaaaagacga cgtcaaactg atcgaagtcc cggttgagta
     2041 catcgcaggt aaagtggttg ctaaagacta tattgatgag tctaccggcg agctgatctg
     2101 cgcagcgaac atggagctga gcctggatct gctggctaag ctgagccagt ctggtcacaa
     2161 gcgtatcgaa acgctgttca ccaacgatct ggatcacggc ccatatatct ctgaaacctt
     2221 acgtgtcgac ccaactaacg accgtctgag cgcactggta gaaatctacc gcatgatgcg
     2281 ccctggcgag ccgccgactc gtgaagcagc tgaaagcctg ttcgagaacc tgttcttctc
     2341 cgaagaccgt tatgacttgt ctgcggttgg tcgtatgaag ttcaaccgtt ctctgctgcg
     2401 cgaagaaatc gaaggttccg gtatcctgag caaagacgac atcattgatg ttatgaaaaa
     2461 gctcatcgat atccgtaacg gtaaaggcga agtcgatgat atcgaccacc tcggcaaccg
     2521 tcgtatccgt tccgttggcg aaatggcgga aaaccagttc cgcgttggcc tggtacgtgt
     2581 agagcgtgcg gtgaaagagc gtctgtctct gggcgatctg gataccctga tgccacagga
     2641 tatgatcaac gccaagccga tttccgcagc agtgaaagag ttcttcggtt ccagccagct
     2701 gtctcagttt atggaccaga acaacccgct gtctgagatt acgcacaaac gtcgtatctc
     2761 cgcactcggc ccaggcggtc tgacccgtga acgtgcaggc ttcgaagttc gagacgtaca
     2821 cccgactcac tacggtcgcg tatgtccaat cgaaacccct gaaggtccga acatcggtct
     2881 gatcaactct ctgtccgtgt acgcacagac taacgaatac ggcttccttg agactccgta
     2941 tcgtaaagtg accgacggtg ttgtaactga cgaaattcac tacctgtctg ctatcgaaga
     3001 aggcaactac gttatcgccc aggcgaactc caacttggat gaagaaggcc acttcgtaga
     3061 agacctggta acttgccgta gcaaaggcga atccagcttg ttcagccgcg accaggttga
     3121 ctacatggac gtatccaccc agcaggtggt atccgtcggt gcgtccctga tcccgttcct
     3181 ggaacacgat gacgccaacc gtgcattgat gggtgcgaac atgcaacgtc aggccgttcc
     3241 gactctgcgc gctgataagc cgctggttgg tactggtatg gaacgtgctg ttgccgttga
     3301 ctccggtgta actgcggtag ctaaacgtgg tggtgtcgtt cagtacgtgg atgcttcccg
     3361 tatcgttatc aaagttaacg aagacgagat gtatccgggt gaagcaggta tcgacatcta
     3421 caacctgacc aaatacaccc gttctaacca gaacacctgt atcaaccaga tgccgtgtgt
     3481 gtctctgggt gaaccggttg aacgtggcga cgtgctggca gacggtccgt ccaccgacct
     3541 cggtgaactg gcgcttggtc agaacatgcg cgtagcgttc atgccgtgga atggttacaa
     3601 cttcgaagac tccatcctcg tatccgagcg tgttgttcag gaagaccgtt tcaccaccat
     3661 ccacattcag gaactggcgt gtgtgtcccg tgacaccaag ctgggtccgg aagagatcac
     3721 cgctgacatc ccgaacgtgg gtgaagctgc gctctccaaa ctggatgaat ccggtatcgt
     3781 ttacattggt gcggaagtga ccggtggcga cattctggtt ggtaaggtaa cgccgaaagg
     3841 tgaaactcag ctgaccccag aagaaaaact gctgcgtgcg atcttcggtg agaaagcctc
     3901 tgacgttaaa gactcttctc tgcgcgtacc aaacggtgta tccggtacgg ttatcgacgt
     3961 tcaggtcttt actcgcgatg gcgtagaaaa agacaaacgt gcgctggaaa tcgaagaaat
     4021 gcagctcaaa caggcgaaga aagacctgtc tgaagaactg cagatcctcg aagcgggtct
     4081 gttcagccgt atccgtgctg tgctggtagc cggtggcgtt gaagctgaga agctcgacaa
     4141 actgccgcgc gatcgctggc tggagctggg cctgacagac gaagagaaac aaaatcagct
     4201 ggaacagctg gctgagcagt atgacgaact gaaacacgag ttcgagaaga aactcgaagc
     4261 gaaacgccgc aaaatcaccc agggcgacga tctggcaccg ggcgtgctga agattgttaa
     4321 ggtatatctg gcggttaaac gccgtatcca gcctggtgac aagatggcag gtcgtcacgg
     4381 taacaagggt gtaatttcta agatcaaccc gatcgaagat atgccttacg atgaaaacgg
     4441 tacgccggta gacatcgtac tgaacccgct gggcgtaccg tctcgtatga acatcggtca
     4501 gatcctcgaa acccacctgg gtatggctgc gaaaggtatc ggcgacaaga tcaacgccat
     4561 gctgaaacag cagcaagaag tcgcgaaact gcgcgaattc atccagcgtg cgtacgatct
     4621 gggcgctgac gttcgtcaga aagttgacct gagtaccttc agcgatgaag aagttatgcg
     4681 tctggctgaa aacctgcgca aaggtatgcc aatcgcaacg ccggtgttcg acggtgcgaa
     4741 agaagcagaa attaaagagc tgctgaaact tggcgacctg ccgacttccg gtcagatccg
     4801 cctgtacgac ggccgcactg gtgaacagtt cgagcgtccg gtaaccgttg gttacatgta
     4861 catgctgaaa ctgaaccacc tggtcgacga caagatgcac gcgcgttcca ccggttctta
     4921 cagcctggtt actcagcagc cgctgggtgg taaggcacag ttcggtggtc agcgtttcgg
     4981 ggagatggaa gtgtgggcgc tggaagcata cggcgcagca tacaccctgc aggaaatgct
     5041 caccgttaag tctgatgacg tgaacggtcg taccaagatg tataaaaaca tcgtggacgg
     5101 caaccatcag atggagccgg gcatgccaga atccttcaac gtattgttga aagagattcg
     5161 ttcgctgggt atcaacatcg aactggaaga cgagtaattc tcgctcaaac aggtcactgc
     5221 tgtcgggtta aaacccggca gcggattgtg ctaactccga cgggagcaaa tccgtgaaag
     5281 atttattaaa gtttctgaaa gcgcagacta aaaccgaaga gtttgatgcg atcaaaattg
     5341 ctctggcttc gccagacatg atccgttcat ggtctttcgg tgaagttaaa aagccggaaa
     5401 ccatcaacta ccgtacgttc aaaccagaac gtgacggcct tttctgcgcc cgtatctttg
     5461 ggccggtaaa agattacgag tgcctgtgcg gtaagtacaa gcgcctgaaa caccgtggcg
     5521 tcatctgtga gaagtgcggc gttgaagtga cccagactaa agtacgccgt gagcgtatgg
     5581 gccacatcga actggcttcc ccgactgcgc acatctggtt cctgaaatcg ctgccgtccc
     5641 gtatcggtct gctgctcgat atgccgctgc gcgatatcga acgcgtactg tactttgaat
     5701 cctatgtggt tatcgaaggc ggtatgacca acctggaacg tcagcagatc ctgactgaag
     5761 agcagtatct ggacgcgctg gaagagttcg gtgacgaatt cgacgcgaag atgggggcgg
     5821 aagcaatcca ggctctgctg aagagcatgg atctggagca agagtgcgaa cagctgcgtg
     5881 aagagctgaa cgaaaccaac tccgaaacca agcgtaaaaa gctgaccaag cgtatcaaac
     5941 tgctggaagc gttcgttcag tctggtaaca aaccagagtg gatgatcctg accgttctgc
     6001 cggtactgcc gccagatctg cgtccgctgg ttccgctgga tggtggtcgt ttcgcgactt
     6061 ctgacctgaa cgatctgtat cgtcgcgtca ttaaccgtaa caaccgtctg aaacgtctgc
     6121 tggatctggc tgcgccggac atcatcgtac gtaacgaaaa acgtatgctg caggaagcgg
     6181 tagacgccct gctggataac ggtcgtcgcg gtcgtgcgat caccggttct aacaagcgtc
     6241 ctctgaaatc tttggccgac atgatcaaag gtaaacaggg tcgtttccgt cagaacctgc
     6301 tcggtaagcg tgttgactac tccggtcgtt ctgtaatcac cgtaggtcca tacctgcgtc
     6361 tgcatcagtg cggtctgccg aagaaaatgg cactggagct gttcaaaccg ttcatctacg
     6421 gcaagctgga actgcgtggt cttgctacca ccattaaagc tgcgaagaaa atggttgagc
     6481 gcgaagaagc tgtcgtttgg gatatcctgg acgaagttat ccgcgaacac ccggtactgc
     6541 tgaaccgtgc accgactctg caccgtctgg gtatccaggc atttgaaccg gtactgatcg
     6601 aaggtaaagc tatccagctg cacccgctgg tttgtgcggc atataacgcc gacttcgatg
     6661 gtgaccagat ggctgttcac gtaccgctga cgctggaagc ccagctggaa gcgcgtgcgc
     6721 tgatgatgtc taccaacaac atcctgtccc cggcgaacgg cgaaccaatc atcgttccgt
     6781 ctcaggacgt tgtactgggt ctgtactaca tgacccgtga ctgtgttaac gccaaaggcg
     6841 aaggcatggt gctgactggc ccgaaagaag cagaacgtct gtatcgctct ggtctggctt
     6901 ctctgcatgc gcgcgttaaa gtgcgtatca ccgagtatga aaaagatgct aacggtgaat
     6961 tagtagcgaa aaccagcctg aaagacacga ctgttggccg tgccattctg tggatgattg
     7021 taccgaaagg tctgccttac tccatcgtca accaggcgct gggtaaaaaa gcaatctcca
     7081 aaatgctgaa cacctgctac cgcattctcg gtctgaaacc gaccgttatt tttgcggacc
     7141 agatcatgta caccggcttc gcctatgcag cgcgttctgg tgcatctgtt ggtatcgatg
     7201 acatggtcat cccggagaag aaacacgaaa tcatctccga ggcagaagca gaagttgctg
     7261 aaattcagga gcagttccag tctggtctgg taactgcggg cgaacgctac aacaaagtta
     7321 tcgatatctg ggctgcggcg aacgatcgtg tatccaaagc gatgatggat aacctgcaaa
     7381 ctgaaaccgt gattaaccgt gacggtcagg aagagaagca ggtttccttc aacagcatct
     7441 acatgatggc cgactccggt gcgcgtggtt ctgcggcaca gattcgtcag cttgctggta
     7501 tgcgtggtct gatggcgaag ccggatggct ccatcatcga aacgccaatc accgcgaact
     7561 tccgtgaagg tctgaacgta ctccagtact tcatctccac ccacggtgct cgtaaaggtc
     7621 tggcggatac cgcactgaaa actgcgaact ccggttacct gactcgtcgt ctggttgacg
     7681 tggcgcagga cctggtggtt accgaagacg attgtggtac ccatgaaggt atcatgatga
     7741 ctccggttat cgagggtggt gacgttaaag agccgctgcg cgatcgcgta ctgggtcgtg
     7801 taactgctga agacgttctg aagccgggta ctgctgatat cctcgttccg cgcaacacgc
     7861 tgctgcacga acagtggtgt gacctgctgg aagagaactc tgtcgacgcg gttaaagtac
     7921 gttctgttgt atcttgtgac accgactttg gtgtatgtgc gcactgctac ggtcgtgacc
     7981 tggcgcgtgg ccacatcatc aacaagggtg aagcaatcgg tgttatcgcg gcacagtcca
     8041 tcggtgaacc gggtacacag ctgaccatgc gtacgttcca catcggtggt gcggcatctc
     8101 gtgcggctgc tgaatccagc atccaagtga aaaacaaagg tagcatcaag ctcagcaacg
     8161 tgaagtcggt tgtgaactcc agcggtaaac tggttatcac ttcccgtaat actgaactga
     8221 aactgatcga cgaattcggt cgtactaaag aaagctacaa agtaccttac ggtgcggtac
     8281 tggcgaaagg cgatggcgaa caggttgctg gcggcgaaac cgttgcaaac tgggacccgc
     8341 acaccatgcc ggttatcacc gaagtaagcg gttttgtacg ctttactgac atgatcgacg
     8401 gccagaccat tacgcgtcag accgacgaac tgaccggtct gtcttcgctg gtggttctgg
     8461 attccgcaga acgtaccgca ggtggtaaag atctgcgtcc ggcactgaaa atcgttgatg
     8521 ctcagggtaa cgacgttctg atcccaggta ccgatatgcc agcgcagtac ttcctgccgg
     8581 gtaaagcgat tgttcagctg gaagatggcg tacagatcag ctctggtgac accctggcgc
     8641 gtattccgca ggaatccggc ggtaccaagg acatcaccgg tggtctgccg cgcgttgcgg
     8701 acctgttcga agcacgtcgt ccgaaagagc cggcaatcct ggctgaaatc agcggtatcg
     8761 tttccttcgg taaagaaacc aaaggtaaac gtcgtctggt tatcaccccg gtagacggta
     8821 gcgatccgta cgaagagatg attccgaaat ggcgtcagct caacgtgttc gaaggtgaac
     8881 gtgtagaacg tggtgacgta atttccgacg gtccggaagc gccgcacgac attctgcgtc
     8941 tgcgtggtgt tcatgctgtt actcgttaca tcgttaacga agtacaggac gtataccgtc
     9001 tgcagggcgt taagattaac gataaacaca tcgaagttat cgttcgtcag atgctgcgta
     9061 aagctaccat cgttaacgcg ggtagctccg acttcctgga aggcgaacag gttgaatact
     9121 ctcgcgtcaa gatcgcaaac cgcgaactgg aagcgaacgg caaagtgggt gcaacttact
     9181 cccgcgatct gctgggtatc accaaagcgt ctctggcaac cgagtccttc atctccgcgg
     9241 catcgttcca ggagaccact cgcgtgctga ccgaagcagc cgttgcgggc aaacgcgacg
     9301 aactgcgcgg cctgaaagag aacgttatcg tgggtcgtct gatcccggca ggtaccggtt
     9361 acgcgtacca ccaggatcgt atgcgtcgcc gtgctgcggg tgaagctccg gctgcaccgc
     9421 aggtgactgc agaagacgca tctgccagcc tggcagaact gctgaacgca ggtctgggcg
     9481 gttctgataa cgagctcgag ctggaagttc tgttccaggg gcccagcagc ggccatcacc
     9541 atcaccatca tcaccatcac cattaaaagc ttattttgag tggactattg aggccagaga
     9601 tctttctctg ttgggttccc atgttcgtaa agctacctac cagacttacg ctccaattct
     9661 ttatgagaat gaccactttt tcgactacat gcaaaaaagt aagtttcatc tcaccattga
     9721 aggtccaaaa gtacttgctt atttacttgg tttatggatt ggtgatggat tgtctgacag
     9781 ggcaactttt tcggttgatt ccagagatac ttctttgatg gaacgtgtta ctgaatatgc
     9841 tgaaaagttg aatttgtgcg ccgagtataa ggacagaaaa gaaccacaag ttgccaaaac
     9901 tgttaatttg tactctaaag ttgtcagagg taatggtatt cgcaataatc ttaatactga
     9961 gaatccatta tgggacgcta ttgttggctt aggattcttg aaggacggtg tcaaaaatat
    10021 tccttctttc ttgtctacgg acaatatcgg tactcgtgaa acatttcttg ctggtctaat
    10081 tgattctgat ggctatgtta ctgatgagca tggtattaaa gcaacaataa agacaattca
    10141 tacttctgtc agagatggtt tggtttccct tgctcgttct ttaggcttag tagtctcggt
    10201 taacgcagaa cctgctaagg ttgacatgaa tgtcaccaaa cataaaatta gttatgctat
    10261 ttatatgtct ggtggagatg ttttgcttaa cgttctttcg aagtgtgccg gctctaaaaa
    10321 attcaggcct gctcccgccg ctgcttttgc acgtgagtgc cgcggatttt atttcgagtt
    10381 acaagaattg aaggaagacg attattatgg gattacttta tctgatgatt ctgatcatca
    10441 gtttttgctt ggatcccagg ttgtcgtcca tgcatgcggt ggcctgaccg gtctgaactc
    10501 aggcctcacg acaaatcctg gtgtatccgc ttggcaggtc aacacagctt atactgcggg
    10561 acaattggtc acatataacg gcaagacgta taaatgtttg cagccccaca cctccttggc
    10621 aggatgggaa ccatccaacg ttcctgcctt gtggcagctt caatgactgc agacttaaga
    10681 aggagattaa tgtatggcac gcgtaactgt tcaggacgct gtagagaaaa ttggtaaccg
    10741 ttttgacctg gtactggtcg ccgcgcgtcg cgcccgtcag atgcaggtag gcggaaagga
    10801 tccgctggta ccggaagaaa acgataaaac cactgtaatc gcgctgcgcg aaatcgaaga
    10861 aggtctgatc aacaaccaga tcctcgacgt tcgcgaacgc caggaacagc aagagcagga
    10921 agccgctgaa ttacaagccg ttaccgctat tgctgaaggt cgtcgttaat ccggataact
    10981 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
    11041 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
    11101 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
    11161 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
    11221 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
    11281 gagagttttc gccccgaaga acgttctcca atgatgagca cttttaaagt tctgctatgt
    11341 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
    11401 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
    11461 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
    11521 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
    11581 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
    11641 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
    11701 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
    11761 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
    11821 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
    11881 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
    11941 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
    12001 atactttaga ttgatttacc ccggttgata atcagaaaag ccccaaaaac aggaagattg
    12061 tataagcaaa tatttaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt
    12121 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
    12181 aagaatagcc cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
    12241 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
    12301 gtgaaccatc acccaaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
    12361 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
    12421 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
    12481 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg taaaaggatc
    12541 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
    12601 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
    12661 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
    12721 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
    12781 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
    12841 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
    12901 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    12961 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    13021 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    13081 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    13141 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    13201 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    13261 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
    13321 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
    13381 cgcagcgagt cagtgagcga ggaagctatg gtgcactctc agtacaatct gctctgatgc
    13441 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
    13501 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
    13561 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
    13621 ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgcag cgattcacag
    13681 atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg
    13741 cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc
    13801 gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc
    13861 acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa
    13921 ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagccgaac
    13981 gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg
    14041 ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg
    14101 aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa
    14161 atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata
    14221 agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct
    14281 ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg
    14341 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
    14401 ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac
    14461 cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa
    14521 gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg
    14581 gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac
    14641 gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac
    14701 cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga
    14761 catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata
    14821 tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag
    14881 cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc
    14941 atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg
    15001 aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat
    15061 gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac
    15121 gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt
    15181 aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat
    15241 cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc
    15301 cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac
    15361 cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac
    15421 tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg
    15481 aaaggttttg cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct
    15541 gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg
    15601 gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca
    15661 cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt
    15721 cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc
    15781 cggcgtagag gatcgagatc tcgatcccgc gaaat
//
