LOCUS       pVSV_G_Addgene13        6508 bp ds-DNA     circular     08-JAN-2020
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     intron          768..1432
                     /locus_tag="Rabbit beta-globin IVS"
                     /ApEinfo_fwdcolor="#ff2831"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1450..2985
                     /locus_tag="VSV-G"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(4851..5712)
                     /locus_tag="AmpR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="#ff6666"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     promoter        24..766
                     /locus_tag="hCMV promoter"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(4074..4756)
                     /locus_tag="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="#999999"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(5975..6281)
                     /locus_tag="F1 ori"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     polyA_signal    3130..3658
                     /locus_tag="Rabbit beta-hemoglobin poly A"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 GCGGCCGCTC TAGAGAGCTT GGCccattgc atacgttgTA TCCATATCAT AATATGTACA
       61 TTTATATTGG CTCATGTCCA ACATTACCGC CATGTTGACA TTGATTATTG ACTAGTTATT
      121 AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA TATGGAGTTC CGCGTTACAT
      181 AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA
      241 TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG
      301 AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC
      361 CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTATGCCCAG TACATGACCT
      421 TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTGA
      481 TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT TGACTCACGG GGATTTCCAA
      541 GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA CCAAAATCAA CGGGACTTTC
      601 CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG
      661 AGGTCTATAT AAGCAGAGCT CGTTTAGTGA ACCGTCAGAT CGCCTGGAGA CGCCATCCAC
      721 GCTGTTTTGA CCTCCATAGA AGACACCGGG ACCGATCCAG CCTCCGGTCG ACCGATCCTG
      781 AGAACTTCAG GGTGAGTTTG GGGACCCTTG ATTGTTCTTT CTTTTTCGCT ATTGTAAAAT
      841 TCATGTTATA TGGAGGGGGC AAAGTTTTCA GGGTGTTGTT TAGAATGGGA AGATGTCCCT
      901 TGTATCACCA TGGACCCTCA TGATAATTTT GTTTCTTTCA CTTTCTACTC TGTTGACAAC
      961 CATTGTCTCC TCTTATTTTC TTTTCATTTT CTGTAACTTT TTCGTTAAAC TTTAGCTTGC
     1021 ATTTGTAACG AATTTTTAAA TTCACTTTTG TTTATTTGTC AGATTGTAAG TACTTTCTCT
     1081 AATCACTTTT TTTTCAAGGC AATCAGGGTA TATTATATTG TACTTCAGCA CAGTTTTAGA
     1141 GAACAATTGT TATAATTAAA TGATAAGGTA GAATATTTCT GCATATAAAT TCTGGCTGGC
     1201 GTGGAAATAT TCTTATTGGT AGAAACAACT ACACCCTGGT CATCATCCTG CCTTTCTCTT
     1261 TATGGTTACA ATGATATACA CTGTTTGAGA TGAGGATAAA ATACTCTGAG TCCAAACCGG
     1321 GCCCCTCTGC TAACCATGTT CATGCCTTCT TCTCTTTCCT ACAGCTCCTG GGCAACGTGC
     1381 TGGTTGTTGT GCTGTCTCAT CATTTTGGCA AAGAATTCCT CGACGGATCC CTCGAGGAAT
     1441 TCTGACACTA TGAAGTGCCT TTTGTACTTA GCCTTTTTAT TCATTGGGGT GAATTGCAAG
     1501 TTCACCATAG TTTTTCCACA CAACCAAAAA GGAAACTGGA AAAATGTTCC TTCTAATTAC
     1561 CATTATTGCC CGTCAAGCTC AGATTTAAAT TGGCATAATG ACTTAATAGG CACAGCCTTA
     1621 CAAGTCAAAA TGCCCAAGAG TCACAAGGCT ATTCAAGCAG ACGGTTGGAT GTGTCATGCT
     1681 TCCAAATGGG TCACTACTTG TGATTTCCGC TGGTATGGAC CGAAGTATAT AACACATTCC
     1741 ATCCGATCCT TCACTCCATC TGTAGAACAA TGCAAGGAAA GCATTGAACA AACGAAACAA
     1801 GGAACTTGGC TGAATCCAGG CTTCCCTCCT CAAAGTTGTG GATATGCAAC TGTGACGGAT
     1861 GCCGAAGCAG TGATTGTCCA GGTGACTCCT CACCATGTGC TGGTTGATGA ATACACAGGA
     1921 GAATGGGTTG ATTCACAGTT CATCAACGGA AAATGCAGCA ATTACATATG CCCCACTGTC
     1981 CATAACTCTA CAACCTGGCA TTCTGACTAT AAGGTCAAAG GGCTATGTGA TTCTAACCTC
     2041 ATTTCCATGG ACATCACCTT CTTCTCAGAG GACGGAGAGC TATCATCCCT GGGAAAGGAG
     2101 GGCACAGGGT TCAGAAGTAA CTACTTTGCT TATGAAACTG GAGGCAAGGC CTGCAAAATG
     2161 CAATACTGCA AGCATTGGGG AGTCAGACTC CCATCAGGTG TCTGGTTCGA GATGGCTGAT
     2221 AAGGATCTCT TTGCTGCAGC CAGATTCCCT GAATGCCCAG AAGGGTCAAG TATCTCTGCT
     2281 CCATCTCAGA CCTCAGTGGA TGTAAGTCTA ATTCAGGACG TTGAGAGGAT CTTGGATTAT
     2341 TCCCTCTGCC AAGAAACCTG GAGCAAAATC AGAGCGGGTC TTCCAATCTC TCCAGTGGAT
     2401 CTCAGCTATC TTGCTCCTAA AAACCCAGGA ACCGGTCCTG CTTTCACCAT AATCAATGGT
     2461 ACCCTAAAAT ACTTTGAGAC CAGATACATC AGAGTCGATA TTGCTGCTCC AATCCTCTCA
     2521 AGAATGGTCG GAATGATCAG TGGAACTACC ACAGAAAGGG AACTGTGGGA TGACTGGGCA
     2581 CCATATGAAG ACGTGGAAAT TGGACCCAAT GGAGTTCTGA GGACCAGTTC AGGATATAAG
     2641 TTTCCTTTAT ACATGATTGG ACATGGTATG TTGGACTCCG ATCTTCATCT TAGCTCAAAG
     2701 GCTCAGGTGT TCGAACATCC TCACATTCAA GACGCTGCTT CGCAACTTCC TGATGATGAG
     2761 AGTTTATTTT TTGGTGATAC TGGGCTATCC AAAAATCCAA TCGAGCTTGT AGAAGGTTGG
     2821 TTCAGTAGTT GGAAAAGCTC TATTGCCTCT TTTTTCTTTA TCATAGGGTT AATCATTGGA
     2881 CTATTCTTGG TTCTCCGAGT TGGTATCCAT CTTTGCATTA AATTAAAGCA CACCAAGAAA
     2941 AGACAGATTT ATACAGACAT AGAGATGAAC CGACTTGGAA AGTAACTCAA ATCCTGCACA
     3001 ACAGATTCTT CATGTTTGGA CCAAATCAAC TTGTGATACC ATGCTCAAAG AGGCCTCAAT
     3061 TATATTTGAG TTTTTAATTT TTATGAAAAA AAAAAAAAAA AACGGAATTC CTCGAGGGAT
     3121 CCGTCGAGGA ATTCACTCCT CAGGTGCAGG CTGCCTATCA gaaggtggtg gctggtgtgg
     3181 ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg
     3241 acatcatgaa gccccttgag catctgactt ctggctAATA AAggaaattt attttcattg
     3301 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
     3361 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg
     3421 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg
     3481 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt
     3541 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat
     3601 ttttcctcct ctcctgacta ctcccagtca tagctGTCCC TCTTCTCTTA TGGAGATCCC
     3661 TCGACGGATC GGCCGCAATT CGTAATCATG TCATAGCTGT TTCCTGTGTG AAATTGTTAT
     3721 CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC
     3781 TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA
     3841 AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT
     3901 ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG
     3961 CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC
     4021 GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG
     4081 TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA
     4141 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC
     4201 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC
     4261 CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG
     4321 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC
     4381 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA
     4441 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG
     4501 AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG
     4561 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT
     4621 GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA
     4681 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA
     4741 GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA
     4801 TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC
     4861 TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA
     4921 CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA
     4981 ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC
     5041 GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT
     5101 TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC
     5161 ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT
     5221 TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGGTTAGCTC
     5281 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     5341 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     5401 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     5461 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     5521 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     5581 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     5641 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     5701 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     5761 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     5821 ATTTCCCCGA AAAGTGCCAC CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA
     5881 AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT
     5941 AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA
     6001 CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC
     6061 CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA
     6121 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG
     6181 GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG
     6241 GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC
     6301 CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA
     6361 TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG
     6421 TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAGC GCGCGTAATA CGACTCACTA
     6481 TAGGGCGAAT TGGAGCTCCA CCGCGGTG   
//
