LOCUS       Exported                7275 bp ds-DNA     circular UNA 31-DEC-2019
DEFINITION  2x BP Genscript ABE7.10 Sa.
ACCESSION   urn.local...c-8wnwdza
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 2, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7275
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     primer_bind     234..253
                     /standard_name="LCv2_seq1"
                     /label=LCv2_seq1
     primer_bind     261..285
                     /standard_name="ACK15"
                     /label=ACK15
     promoter        275..356
                     /note="Geneious type: Promoter"
     primer_bind     286..306
                     /standard_name="Genewiz CMV-Forward"
                     /label=Genewiz CMV-Forward
     primer_bind     293..314
                     /standard_name="QB0976 pRLuc-MCS-F"
                     /label=QB0976 pRLuc-MCS-F
     primer_bind     complement(331..360)
                     /standard_name="352_CMV_Rev2"
                     /label=352_CMV_Rev2
     primer_bind     377..396
                     /standard_name="T7 promoter"
                     /label=T7 promoter
     promoter        377..395
                     /note="Geneious type: Promoter"
     misc_feature    406..408
                     /standard_name="USER1"
                     /label=USER1
     CDS             409..465
                     /standard_name="ABE 7.10"
                     /label=ABE 7.10
     misc_feature    412..430
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
     misc_feature    432..4280
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
     CDS             466..963
                     /standard_name="max10"
                     /label=max10
     misc_feature    964..1059
                     /standard_name="2xSGGS-XTEN-2xSGGS"
                     /label=2xSGGS-XTEN-2xSGGS
                     /note="Geneious type: Misc"
     primer_bind     complement(1044..1076)
                     /standard_name="TPH721"
                     /label=TPH721
     CDS             1060..4218
                     /standard_name="SaCas9"
                     /label=SaCas9
     primer_bind     1063..1110
                     /standard_name="TPH494"
                     /label=TPH494
     misc_feature    1063..1076
                     /standard_name="USER Junction"
                     /label=USER Junction
     primer_bind     complement(1076..1100)
                     /standard_name="TPH566"
                     /label=TPH566
     modified_base   1087..1089
     misc_feature    complement(1512..1539)
                     /standard_name="KZ1445"
                     /label=KZ1445
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     2915..2941
                     /standard_name="KZ1446"
                     /label=KZ1446
     primer_bind     complement(4180..4208)
                     /standard_name="TPH722"
                     /label=TPH722
     primer_bind     4194..4239
                     /standard_name="TPH723"
                     /label=TPH723
     misc_feature    4194..4208
                     /standard_name="USER Junction"
                     /label=USER Junction
     CDS             4231..4281
                     /standard_name="BPNLS"
                     /label=BPNLS
     primer_bind     4282..4305
                     /standard_name="202_bb_NpuN1_F"
                     /label=202_bb_NpuN1_F
     primer_bind     4282..4302
                     /standard_name="129_CMV_fwd"
                     /label=129_CMV_fwd
     primer_bind     4285..4305
                     /standard_name="468_bb-rev-gfpnls"
                     /label=468_bb-rev-gfpnls
     primer_bind     4326..4348
                     /standard_name="304_FP-JL11_F"
                     /label=304_FP-JL11_F
     primer_bind     complement(4330..4347)
                     /standard_name="Genewiz BGHR"
                     /label=Genewiz BGHR
     primer_bind     complement(4330..4347)
                     /standard_name="Eton BGH Reverse"
                     /label=Eton BGH Reverse
     primer_bind     complement(4330..4347)
                     /standard_name="QB0008 BGH Reverse"
                     /label=QB0008 BGH Reverse
     terminator      4333..4560
                     /note="Geneious type: Terminator"
     primer_bind     4333..4353
                     /standard_name="435_bGH_QPCRF1"
                     /label=435_bGH_QPCRF1
     primer_bind     4359..4377
                     /standard_name="436_bGH_QPCRF2"
                     /label=436_bGH_QPCRF2
     primer_bind     complement(4482..4506)
                     /standard_name="ACK64 (end)"
                     /label=ACK64 (end)
     misc_feature    complement(4482..4506)
                     /standard_name="ACK64"
                     /label=ACK64
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(4496..4515)
                     /standard_name="438_bGH_QPCRR2"
                     /label=438_bGH_QPCRR2
     primer_bind     complement(4501..4519)
                     /standard_name="439_bGH_QPCRR3"
                     /label=439_bGH_QPCRR3
     primer_bind     complement(4521..4540)
                     /standard_name="437_bGH_QPCRR1"
                     /label=437_bGH_QPCRR1
     primer_bind     complement(4541..4560)
                     /standard_name="920_bGH-BamHI-rev"
                     /label=920_bGH-BamHI-rev
     primer_bind     4619..4645
                     /standard_name="654_CAG_fwd"
                     /label=654_CAG_fwd
     primer_bind     complement(4631..4647)
                     /standard_name="Genewiz M13R"
                     /label=Genewiz M13R
     primer_bind     complement(4631..4647)
                     /standard_name="QB0048 M13 Reverse"
                     /label=QB0048 M13 Reverse
     primer_bind     complement(4631..4647)
                     /standard_name="Eton M13R Reverse"
                     /label=Eton M13R Reverse
     primer_bind     complement(4631..4647)
                     /standard_name="M13-R (-26)"
                     /label=M13-R (-26)
     primer_bind     complement(4644..4667)
                     /standard_name="QB0108 Tac promoter"
                     /label=QB0108 Tac promoter
     primer_bind     complement(4644..4667)
                     /standard_name="M13-R (-46)"
                     /label=M13-R (-46)
     primer_bind     complement(4644..4666)
                     /standard_name="QB0050 M13/pUC Reverse"
                     /label=QB0050 M13/pUC Reverse
     promoter        complement(4680..4709)
                     /note="Geneious type: Promoter"
     primer_bind     complement(4716..4735)
                     /standard_name="836_Lac-F"
                     /label=836_Lac-F
     primer_bind     complement(4716..4735)
                     /standard_name="Lac-F (EtonSeq)"
                     /label=Lac-F (EtonSeq)
     primer_bind     4724..4748
                     /standard_name="ACK21"
                     /label=ACK21
     misc_feature    complement(4724..4748)
                     /standard_name="ACK32"
                     /label=ACK32
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(4862..4879)
                     /standard_name="QB0039 L4440"
                     /label=QB0039 L4440
     primer_bind     complement(4914..4933)
                     /standard_name="VR"
                     /label=VR
     primer_bind     complement(5003..5024)
                     /standard_name="11_pFYF_Seq_Rev"
                     /label=11_pFYF_Seq_Rev
     misc_feature    complement(5018..5637)
                     /standard_name="pBR322 origin"
                     /label=pBR322 origin
                     /note="Geneious type: Origin of Replication"
     primer_bind     5033..5057
                     /standard_name="ACK10"
                     /label=ACK10
     primer_bind     complement(5094..5113)
                     /standard_name="BR322-F"
                     /label=BR322-F
     misc_feature    5229
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
     primer_bind     5256..5274
                     /standard_name="QB0766 RepO-F"
                     /label=QB0766 RepO-F
     primer_bind     5353..5372
                     /standard_name="833_BR322-rev1"
                     /label=833_BR322-rev1
     primer_bind     complement(5381..5400)
                     /standard_name="QB0767 RepO-R"
                     /label=QB0767 RepO-R
     misc_feature    5474
                     /standard_name="G"
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     primer_bind     5534..5558
                     /standard_name="ACK11"
                     /label=ACK11
     primer_bind     complement(5534..5553)
                     /standard_name="832_BR322-fwd1"
                     /label=832_BR322-fwd1
     misc_feature    complement(5792..6652)
                     /standard_name="amp marker"
                     /label=amp marker
                     /note="Geneious type: Marker"
     primer_bind     6033..6057
                     /standard_name="ACK12"
                     /label=ACK12
     primer_bind     6198..6217
                     /standard_name="ACK563"
                     /label=ACK563
     primer_bind     6415..6434
                     /standard_name="QB0005 Amp-R"
                     /label=QB0005 Amp-R
     primer_bind     complement(6500..6527)
                     /standard_name="562_Amp_Fwd"
                     /label=562_Amp_Fwd
     primer_bind     6500..6527
                     /standard_name="563_Amp_Rev"
                     /label=563_Amp_Rev
     primer_bind     6533..6557
                     /standard_name="ACK13"
                     /label=ACK13
     promoter        complement(6694..6722)
                     /note="Geneious type: Promoter"
     primer_bind     6766..6783
                     /standard_name="QB0090 pQE promoter"
                     /label=QB0090 pQE promoter
     primer_bind     complement(6836..6855)
                     /standard_name="QB0092 pRS-marker"
                     /label=QB0092 pRS-marker
     primer_bind     7036..7060
                     /standard_name="JLseq006_ACK14"
                     /label=JLseq006_ACK14
     primer_bind     7036..7060
                     /standard_name="ACK14"
                     /label=ACK14
     promoter        join(7043..7275,1..356)
     primer_bind     7043..7086
                     /standard_name="387_pAAV_fwd"
                     /label=387_pAAV_fwd
     primer_bind     complement(7093..7112)
                     /standard_name="QB1110 pSicoR"
                     /label=QB1110 pSicoR
     misc_feature    7232
                     /standard_name="C"
                     /label=C
                     /note="Geneious type: Editing History Replacement"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctctga ggtggagttt
      481 tcccacgagt actggatgag acatgccctg accctggcca agagggcacg ggatgagagg
      541 gaggtgcctg tgggagccgt gctggtgctg aacaatagag tgatcggcga gggctggaac
      601 agagccatcg gcctgcacga cccaacagcc catgccgaaa ttatggccct gagacagggc
      661 ggcctggtca tgcagaacta cagactgatt gacgccaccc tgtacgtgac attcgagcct
      721 tgcgtgatgt gcgccggcgc catgatccac tctaggatcg gccgcgtggt gtttggcgtg
      781 aggaactcaa aaagaggcgc cgcaggctcc ctgatgaacg tgctgaacta ccccggcatg
      841 aatcaccgcg tcgaaattac cgagggaatc ctggcagatg aatgtgccgc cctgctgtgc
      901 gatttctatc ggatgcctag acaggtgttc aatgctcaga agaaggccca gagctccatc
      961 aactccggag gatctagcgg aggctcctct ggctctgaga cacctggcac aagcgagagc
     1021 gcaacacctg aaagcagcgg gggcagcagc ggggggtcag ggaagcgaaa ttacattctg
     1081 gggctggcca ttggcattac atcagtgggc tatggcatca ttgactacga gacaagggac
     1141 gtgatcgacg ccggcgtgag actgttcaag gaggccaacg tggagaacaa tgagggccgg
     1201 agatccaaga ggggagcaag gcgcctgaag cggagaaggc gccacagaat ccagagagtg
     1261 aagaagctgc tgttcgatta caacctgctg accgaccact ccgagctgtc tggcatcaat
     1321 ccttatgagg ccagagtgaa gggcctgtcc cagaagctgt ctgaggagga gtttagcgcc
     1381 gccctgctgc acctggcaaa gaggagaggc gtgcacaacg tgaatgaggt ggaggaggac
     1441 accggcaacg agctgtccac aaaggagcag atcagccgca attccaaggc cctggaggag
     1501 aagtatgtgg ccgagctgca gctggagcgg ctgaagaagg atggcgaggt gaggggctcc
     1561 atcaatcgct tcaagacctc tgactacgtg aaggaggcca agcagctgct gaaggtgcag
     1621 aaggcctacc accagctgga tcagtccttt atcgatacat atatcgacct gctggagaca
     1681 aggcgcacat actatgaggg accaggagag ggctctccct tcggctggaa ggacatcaag
     1741 gagtggtacg agatgctgat gggccactgc acctattttc cagaggagct gagaagcgtg
     1801 aagtacgcct ataacgccga tctgtacaac gccctgaatg acctgaacaa cctggtcatc
     1861 accagggatg agaacgagaa gctggagtac tatgagaagt tccagatcat cgagaacgtg
     1921 ttcaagcaga agaagaagcc tacactgaag cagatcgcca aggagatcct ggtgaacgag
     1981 gaggacatca agggctaccg cgtgacctcc acaggcaagc cagagttcac caatctgaag
     2041 gtgtatcacg atatcaagga catcacagcc cggaaggaga tcatcgagaa cgccgagctg
     2101 ctggatcaga tcgccaagat cctgaccatc tatcagagct ccgaggacat ccaggaggag
     2161 ctgaccaacc tgaatagcga gctgacacag gaggagatcg agcagatcag caatctgaag
     2221 ggctacaccg gcacacacaa cctgagcctg aaggccatca atctgatcct ggatgagctg
     2281 tggcacacaa acgacaatca gatcgccatc tttaaccggc tgaagctggt gccaaagaag
     2341 gtggacctgt cccagcagaa ggagatccca accacactgg tggacgattt catcctgtct
     2401 cccgtggtga agcggagctt catccagagc atcaaagtga tcaacgccat catcaagaag
     2461 tacggcctgc ccaatgatat catcatcgag ctggccaggg agaagaactc caaggacgcc
     2521 cagaagatga tcaatgagat gcagaagagg aaccgccaga ccaatgagcg gatcgaggag
     2581 atcatcagaa ccacaggcaa ggagaacgcc aagtacctga tcgagaagat caagctgcac
     2641 gatatgcagg agggcaagtg tctgtattct ctggaggcca tccctctgga ggacctgctg
     2701 aacaatccat tcaactacga ggtggatcac atcatccccc ggagcgtgag cttcgacaat
     2761 tcttttaaca ataaggtgct ggtgaagcag gaggagaaca gcaagaaggg caataggacc
     2821 cctttccagt acctgtctag ctccgattct aagatcagct acgagacatt caagaagcac
     2881 atcctgaatc tggccaaggg caagggccgc atcagcaaga ccaagaagga gtacctgctg
     2941 gaggagcggg acatcaacag attctccgtg cagaaggact tcatcaaccg gaatctggtg
     3001 gacaccagat acgccacacg cggcctgatg aatctgctgc ggtcttattt cagagtgaac
     3061 aatctggatg tgaaggtgaa gagcatcaac ggcggcttca cctcctttct gcggagaaag
     3121 tggaagttta agaaggagcg caacaagggc tataagcacc acgccgagga tgccctgatc
     3181 atcgccaatg ccgacttcat ctttaaggag tggaagaagc tggacaaggc caagaaagtg
     3241 atggagaacc agatgttcga ggagaagcag gccgagagca tgcccgagat cgagacagag
     3301 caggagtaca aggagatttt catcacacct caccagatca agcacatcaa ggacttcaag
     3361 gactacaagt attctcacag ggtggataag aagcccaacc gcaagctgat caatgacacc
     3421 ctgtatagca cacggaagga cgataagggc aataccctga tcgtgaacaa tctgaacggc
     3481 ctgtacgaca aggataatga caagctgaag aagctgatca acaagtctcc cgagaagctg
     3541 ctgatgtacc accacgatcc tcagacatat cagaagctga agctgatcat ggagcagtac
     3601 ggcgacgaga agaacccact gtataagtac tatgaggaga caggcaacta cctgacaaag
     3661 tatagcaaga aggataatgg ccccgtgatc aagaagatca agtactatgg caacaagctg
     3721 aatgcccacc tggacatcac cgacgattac cctaactctc gcaataaggt ggtgaagctg
     3781 agcctgaagc cataccggtt cgacgtgtac ctggacaacg gcgtgtataa gtttgtgaca
     3841 gtgaagaatc tggatgtgat caagaaggag aactactatg aggtgaacag caagtgctac
     3901 gaggaggcca agaagctgaa gaagatcagc aaccaggccg agttcatcgc ctctttttac
     3961 aagaatgacc tgatcaagat caatggcgag ctgtatagag tgatcggcgt gaacaatgat
     4021 ctgctgaaca gaatcgaagt gaatatgatc gacatcacct acagggagta tctggagaac
     4081 atgaatgata agaggccccc tcatatcatc aagaccatcg cctctaagac acagagcatc
     4141 aagaagtaca gcacagacat cctggggaac ctgtatgaag tcaagagcaa gaaacatcct
     4201 cagattatca agaaaggctc tggcggctca aaaagaaccg ccgacggcag cgaattcgag
     4261 cccaagaaga agaggaaagt ctaaccggtc atcatcacca tcaccattga gtttaaaccc
     4321 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     4381 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     4441 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     4501 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     4561 cttctgaggc ggaaagaacc agctggggct cgataccgtc gacctctagc tagagcttgg
     4621 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     4681 acatacgagc cggaagcata aagtgtaaag cctagggtgc ctaatgagtg agctaactca
     4741 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     4801 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     4861 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     4921 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     4981 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     5041 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     5101 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     5161 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     5221 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     5281 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5341 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     5401 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5461 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5521 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5581 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5641 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     5701 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     5761 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     5821 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     5881 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     5941 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     6001 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     6061 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     6121 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     6181 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     6241 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     6301 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     6361 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6421 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6481 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6541 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6601 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6661 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6721 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     6781 ctgacgtcga cggatcggga gatcgatctc ccgatcccct agggtcgact ctcagtacaa
     6841 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     6901 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     6961 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
     7021 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     7081 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     7141 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     7201 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     7261 tacatcaagt gtatc
//
