LOCUS Exported 7869 bp ds-DNA circular UNA 31-DEC-2019 DEFINITION 2x BP Genscript ABE7.10 Sa. ACCESSION urn.local...c-8wnwdza VERSION . KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified REFERENCE 1 (bases 1 to 7869) AUTHORS . TITLE Direct Submission JOURNAL Exported Thursday, Jan 2, 2020 from SnapGene Viewer 5.0.7 https://www.snapgene.com FEATURES Location/Qualifiers source 1..7869 /organism="unspecified" /mol_type="genomic DNA" primer_bind 234..253 /standard_name="LCv2_seq1" /label=LCv2_seq1 primer_bind 261..285 /standard_name="ACK15" /label=ACK15 promoter 275..356 /note="Geneious type: Promoter" primer_bind 286..306 /standard_name="Genewiz CMV-Forward" /label=Genewiz CMV-Forward primer_bind 293..314 /standard_name="QB0976 pRLuc-MCS-F" /label=QB0976 pRLuc-MCS-F primer_bind complement(331..360) /standard_name="352_CMV_Rev2" /label=352_CMV_Rev2 primer_bind 377..396 /standard_name="T7 promoter" /label=T7 promoter promoter 377..395 /note="Geneious type: Promoter" misc_feature 406..408 /standard_name="USER1" /label=USER1 CDS 409..1059 /standard_name="ABE 7.10" /label=ABE 7.10 misc_feature 412..430 /label=Geneious type: Editing History Insertion /note="Geneious type: Editing History Insertion" primer_bind 428..487 /standard_name="925_Genscript_ABE-BPNLS-fwd" /label=925_Genscript_ABE-BPNLS-fwd misc_feature 432..4874 /label=Geneious type: Editing History Insertion /note="Geneious type: Editing History Insertion" misc_feature 964..1059 /standard_name="2xSGGS-XTEN-2xSGGS" /label=2xSGGS-XTEN-2xSGGS /note="Geneious type: Misc" CDS 1060..1557 /standard_name="max10" /label=max10 misc_feature 1558..1653 /standard_name="2xSGGS-XTEN-2xSGGS" /label=2xSGGS-XTEN-2xSGGS /note="Geneious type: Misc" primer_bind complement(1638..1670) /standard_name="TPH721" /label=TPH721 CDS 1654..4812 /standard_name="SaCas9" /label=SaCas9 primer_bind 1657..1704 /standard_name="TPH494" /label=TPH494 misc_feature 1657..1670 /standard_name="USER Junction" /label=USER Junction primer_bind complement(1670..1694) /standard_name="TPH566" /label=TPH566 modified_base 1681..1683 primer_bind complement(4774..4802) /standard_name="TPH722" /label=TPH722 primer_bind 4788..4833 /standard_name="TPH723" /label=TPH723 misc_feature 4788..4802 /standard_name="USER Junction" /label=USER Junction CDS 4825..4875 /standard_name="BPNLS" /label=BPNLS primer_bind 4876..4899 /standard_name="202_bb_NpuN1_F" /label=202_bb_NpuN1_F primer_bind 4876..4896 /standard_name="129_CMV_fwd" /label=129_CMV_fwd primer_bind 4879..4899 /standard_name="468_bb-rev-gfpnls" /label=468_bb-rev-gfpnls primer_bind 4920..4942 /standard_name="304_FP-JL11_F" /label=304_FP-JL11_F primer_bind complement(4924..4941) /standard_name="Genewiz BGHR" /label=Genewiz BGHR primer_bind complement(4924..4941) /standard_name="Eton BGH Reverse" /label=Eton BGH Reverse primer_bind complement(4924..4941) /standard_name="QB0008 BGH Reverse" /label=QB0008 BGH Reverse terminator 4927..5154 /note="Geneious type: Terminator" primer_bind 4927..4947 /standard_name="435_bGH_QPCRF1" /label=435_bGH_QPCRF1 primer_bind 4953..4971 /standard_name="436_bGH_QPCRF2" /label=436_bGH_QPCRF2 primer_bind complement(5076..5100) /standard_name="ACK64 (end)" /label=ACK64 (end) misc_feature complement(5076..5100) /standard_name="ACK64" /label=ACK64 /note="Geneious type: primer_bind_reverse" primer_bind complement(5090..5109) /standard_name="438_bGH_QPCRR2" /label=438_bGH_QPCRR2 primer_bind complement(5095..5113) /standard_name="439_bGH_QPCRR3" /label=439_bGH_QPCRR3 primer_bind complement(5115..5134) /standard_name="437_bGH_QPCRR1" /label=437_bGH_QPCRR1 primer_bind complement(5135..5154) /standard_name="920_bGH-BamHI-rev" /label=920_bGH-BamHI-rev primer_bind 5213..5239 /standard_name="654_CAG_fwd" /label=654_CAG_fwd primer_bind complement(5225..5241) /standard_name="Genewiz M13R" /label=Genewiz M13R primer_bind complement(5225..5241) /standard_name="QB0048 M13 Reverse" /label=QB0048 M13 Reverse primer_bind complement(5225..5241) /standard_name="Eton M13R Reverse" /label=Eton M13R Reverse primer_bind complement(5225..5241) /standard_name="M13-R (-26)" /label=M13-R (-26) primer_bind complement(5238..5261) /standard_name="QB0108 Tac promoter" /label=QB0108 Tac promoter primer_bind complement(5238..5261) /standard_name="M13-R (-46)" /label=M13-R (-46) primer_bind complement(5238..5260) /standard_name="QB0050 M13/pUC Reverse" /label=QB0050 M13/pUC Reverse promoter complement(5274..5303) /note="Geneious type: Promoter" primer_bind complement(5310..5329) /standard_name="836_Lac-F" /label=836_Lac-F primer_bind complement(5310..5329) /standard_name="Lac-F (EtonSeq)" /label=Lac-F (EtonSeq) primer_bind 5318..5342 /standard_name="ACK21" /label=ACK21 misc_feature complement(5318..5342) /standard_name="ACK32" /label=ACK32 /note="Geneious type: primer_bind_reverse" primer_bind complement(5456..5473) /standard_name="QB0039 L4440" /label=QB0039 L4440 primer_bind complement(5508..5527) /standard_name="VR" /label=VR primer_bind complement(5597..5618) /standard_name="11_pFYF_Seq_Rev" /label=11_pFYF_Seq_Rev misc_feature complement(5612..6231) /standard_name="pBR322 origin" /label=pBR322 origin /note="Geneious type: Origin of Replication" primer_bind 5627..5651 /standard_name="ACK10" /label=ACK10 primer_bind complement(5688..5707) /standard_name="BR322-F" /label=BR322-F misc_feature 5823 /label=Geneious type: Editing History Insertion /note="Geneious type: Editing History Insertion" primer_bind 5850..5868 /standard_name="QB0766 RepO-F" /label=QB0766 RepO-F primer_bind 5947..5966 /standard_name="833_BR322-rev1" /label=833_BR322-rev1 primer_bind complement(5975..5994) /standard_name="QB0767 RepO-R" /label=QB0767 RepO-R misc_feature 6068 /standard_name="G" /label=G /note="Geneious type: Editing History Replacement" primer_bind 6128..6152 /standard_name="ACK11" /label=ACK11 primer_bind complement(6128..6147) /standard_name="832_BR322-fwd1" /label=832_BR322-fwd1 misc_feature complement(6386..7246) /standard_name="amp marker" /label=amp marker /note="Geneious type: Marker" primer_bind 6627..6651 /standard_name="ACK12" /label=ACK12 primer_bind 6792..6811 /standard_name="ACK563" /label=ACK563 primer_bind 7009..7028 /standard_name="QB0005 Amp-R" /label=QB0005 Amp-R primer_bind complement(7094..7121) /standard_name="562_Amp_Fwd" /label=562_Amp_Fwd primer_bind 7094..7121 /standard_name="563_Amp_Rev" /label=563_Amp_Rev primer_bind 7127..7151 /standard_name="ACK13" /label=ACK13 promoter complement(7288..7316) /note="Geneious type: Promoter" primer_bind 7360..7377 /standard_name="QB0090 pQE promoter" /label=QB0090 pQE promoter primer_bind complement(7430..7449) /standard_name="QB0092 pRS-marker" /label=QB0092 pRS-marker primer_bind 7630..7654 /standard_name="JLseq006_ACK14" /label=JLseq006_ACK14 primer_bind 7630..7654 /standard_name="ACK14" /label=ACK14 promoter join(7637..7869,1..356) primer_bind 7637..7680 /standard_name="387_pAAV_fwd" /label=387_pAAV_fwd primer_bind complement(7687..7706) /standard_name="QB1110 pSicoR" /label=QB1110 pSicoR misc_feature 7826 /standard_name="C" /label=C /note="Geneious type: Editing History Replacement" ORIGIN 1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctctga agtcgagttt 481 agccacgagt attggatgag gcacgcactg accctggcaa agcgagcatg ggatgaaaga 541 gaagtccccg tgggcgccgt gctggtgcac aacaatagag tgatcggaga gggatggaac 601 aggccaatcg gccgccacga ccctaccgca cacgcagaga tcatggcact gaggcaggga 661 ggcctggtca tgcagaatta ccgcctgatc gatgccaccc tgtatgtgac actggagcca 721 tgcgtgatgt gcgcaggagc aatgatccac agcaggatcg gaagagtggt gttcggagca 781 cgggacgcca agaccggcgc agcaggctcc ctgatggatg tgctgcacca ccccggcatg 841 aaccaccggg tggagatcac agagggaatc ctggcagacg agtgcgccgc cctgctgagc 901 gatttcttta gaatgcggag acaggagatc aaggcccaga agaaggcaca gagctccacc 961 gactctggag gatctagcgg aggatcctct ggaagcgaga caccaggcac aagcgagtcc 1021 gccacaccag agagctccgg cggctcctcc ggaggatcct ctgaggtgga gttttcccac 1081 gagtactgga tgagacatgc cctgaccctg gccaagaggg cacgggatga gagggaggtg 1141 cctgtgggag ccgtgctggt gctgaacaat agagtgatcg gcgagggctg gaacagagcc 1201 atcggcctgc acgacccaac agcccatgcc gaaattatgg ccctgagaca gggcggcctg 1261 gtcatgcaga actacagact gattgacgcc accctgtacg tgacattcga gccttgcgtg 1321 atgtgcgccg gcgccatgat ccactctagg atcggccgcg tggtgtttgg cgtgaggaac 1381 tcaaaaagag gcgccgcagg ctccctgatg aacgtgctga actaccccgg catgaatcac 1441 cgcgtcgaaa ttaccgaggg aatcctggca gatgaatgtg ccgccctgct gtgcgatttc 1501 tatcggatgc ctagacaggt gttcaatgct cagaagaagg cccagagctc catcaactcc 1561 ggaggatcta gcggaggctc ctctggctct gagacacctg gcacaagcga gagcgcaaca 1621 cctgaaagca gcgggggcag cagcgggggg tcagggaagc gaaattacat tctggggctg 1681 gccattggca ttacatcagt gggctatggc atcattgact acgagacaag ggacgtgatc 1741 gacgccggcg tgagactgtt caaggaggcc aacgtggaga acaatgaggg ccggagatcc 1801 aagaggggag caaggcgcct gaagcggaga aggcgccaca gaatccagag agtgaagaag 1861 ctgctgttcg attacaacct gctgaccgac cactccgagc tgtctggcat caatccttat 1921 gaggccagag tgaagggcct gtcccagaag ctgtctgagg aggagtttag cgccgccctg 1981 ctgcacctgg caaagaggag aggcgtgcac aacgtgaatg aggtggagga ggacaccggc 2041 aacgagctgt ccacaaagga gcagatcagc cgcaattcca aggccctgga ggagaagtat 2101 gtggccgagc tgcagctgga gcggctgaag aaggatggcg aggtgagggg ctccatcaat 2161 cgcttcaaga cctctgacta cgtgaaggag gccaagcagc tgctgaaggt gcagaaggcc 2221 taccaccagc tggatcagtc ctttatcgat acatatatcg acctgctgga gacaaggcgc 2281 acatactatg agggaccagg agagggctct cccttcggct ggaaggacat caaggagtgg 2341 tacgagatgc tgatgggcca ctgcacctat tttccagagg agctgagaag cgtgaagtac 2401 gcctataacg ccgatctgta caacgccctg aatgacctga acaacctggt catcaccagg 2461 gatgagaacg agaagctgga gtactatgag aagttccaga tcatcgagaa cgtgttcaag 2521 cagaagaaga agcctacact gaagcagatc gccaaggaga tcctggtgaa cgaggaggac 2581 atcaagggct accgcgtgac ctccacaggc aagccagagt tcaccaatct gaaggtgtat 2641 cacgatatca aggacatcac agcccggaag gagatcatcg agaacgccga gctgctggat 2701 cagatcgcca agatcctgac catctatcag agctccgagg acatccagga ggagctgacc 2761 aacctgaata gcgagctgac acaggaggag atcgagcaga tcagcaatct gaagggctac 2821 accggcacac acaacctgag cctgaaggcc atcaatctga tcctggatga gctgtggcac 2881 acaaacgaca atcagatcgc catctttaac cggctgaagc tggtgccaaa gaaggtggac 2941 ctgtcccagc agaaggagat cccaaccaca ctggtggacg atttcatcct gtctcccgtg 3001 gtgaagcgga gcttcatcca gagcatcaaa gtgatcaacg ccatcatcaa gaagtacggc 3061 ctgcccaatg atatcatcat cgagctggcc agggagaaga actccaagga cgcccagaag 3121 atgatcaatg agatgcagaa gaggaaccgc cagaccaatg agcggatcga ggagatcatc 3181 agaaccacag gcaaggagaa cgccaagtac ctgatcgaga agatcaagct gcacgatatg 3241 caggagggca agtgtctgta ttctctggag gccatccctc tggaggacct gctgaacaat 3301 ccattcaact acgaggtgga tcacatcatc ccccggagcg tgagcttcga caattctttt 3361 aacaataagg tgctggtgaa gcaggaggag aacagcaaga agggcaatag gacccctttc 3421 cagtacctgt ctagctccga ttctaagatc agctacgaga cattcaagaa gcacatcctg 3481 aatctggcca agggcaaggg ccgcatcagc aagaccaaga aggagtacct gctggaggag 3541 cgggacatca acagattctc cgtgcagaag gacttcatca accggaatct ggtggacacc 3601 agatacgcca cacgcggcct gatgaatctg ctgcggtctt atttcagagt gaacaatctg 3661 gatgtgaagg tgaagagcat caacggcggc ttcacctcct ttctgcggag aaagtggaag 3721 tttaagaagg agcgcaacaa gggctataag caccacgccg aggatgccct gatcatcgcc 3781 aatgccgact tcatctttaa ggagtggaag aagctggaca aggccaagaa agtgatggag 3841 aaccagatgt tcgaggagaa gcaggccgag agcatgcccg agatcgagac agagcaggag 3901 tacaaggaga ttttcatcac acctcaccag atcaagcaca tcaaggactt caaggactac 3961 aagtattctc acagggtgga taagaagccc aaccgcaagc tgatcaatga caccctgtat 4021 agcacacgga aggacgataa gggcaatacc ctgatcgtga acaatctgaa cggcctgtac 4081 gacaaggata atgacaagct gaagaagctg atcaacaagt ctcccgagaa gctgctgatg 4141 taccaccacg atcctcagac atatcagaag ctgaagctga tcatggagca gtacggcgac 4201 gagaagaacc cactgtataa gtactatgag gagacaggca actacctgac aaagtatagc 4261 aagaaggata atggccccgt gatcaagaag atcaagtact atggcaacaa gctgaatgcc 4321 cacctggaca tcaccgacga ttaccctaac tctcgcaata aggtggtgaa gctgagcctg 4381 aagccatacc ggttcgacgt gtacctggac aacggcgtgt ataagtttgt gacagtgaag 4441 aatctggatg tgatcaagaa ggagaactac tatgaggtga acagcaagtg ctacgaggag 4501 gccaagaagc tgaagaagat cagcaaccag gccgagttca tcgcctcttt ttacaagaat 4561 gacctgatca agatcaatgg cgagctgtat agagtgatcg gcgtgaacaa tgatctgctg 4621 aacagaatcg aagtgaatat gatcgacatc acctacaggg agtatctgga gaacatgaat 4681 gataagaggc cccctcatat catcaagacc atcgcctcta agacacagag catcaagaag 4741 tacagcacag acatcctggg gaacctgtat gaagtcaaga gcaagaaaca tcctcagatt 4801 atcaagaaag gctctggcgg ctcaaaaaga accgccgacg gcagcgaatt cgagcccaag 4861 aagaagagga aagtctaacc ggtcatcatc accatcacca ttgagtttaa acccgctgat 4921 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt 4981 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat 5041 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg 5101 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg 5161 aggcggaaag aaccagctgg ggctcgatac cgtcgacctc tagctagagc ttggcgtaat 5221 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 5281 gagccggaag cataaagtgt aaagcctagg gtgcctaatg agtgagctaa ctcacattaa 5341 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat 5401 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc 5461 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 5521 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 5581 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 5641 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 5701 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 5761 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 5821 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 5881 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 5941 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 6001 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 6061 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 6121 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 6181 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 6241 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 6301 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 6361 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 6421 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 6481 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 6541 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 6601 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 6661 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 6721 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 6781 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 6841 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 6901 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 6961 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 7021 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 7081 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 7141 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 7201 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 7261 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 7321 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 7381 tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt acaatctgct 7441 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt 7501 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga 7561 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt 7621 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 7681 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 7741 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 7801 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 7861 aagtgtatc //