LOCUS       Exported                8037 bp ds-DNA     circular UNA 31-DEC-2019
DEFINITION  ABEmax10.
ACCESSION   urn.local...c-8wnwdza
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 2, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8037
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     source          1060..4980
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          4981..4992
     primer_bind     234..253
                     /standard_name="LCv2_seq1"
                     /label=LCv2_seq1
     primer_bind     261..285
                     /standard_name="JLseq007_ACK15"
                     /label=JLseq007_ACK15
     primer_bind     261..285
                     /standard_name="ACK15"
                     /label=ACK15
     promoter        275..356
                     /note="Geneious type: Promoter"
     primer_bind     286..306
                     /standard_name="QB0012 CMV Forward"
                     /label=QB0012 CMV Forward
     primer_bind     286..306
                     /standard_name="Eton CMV-Forward"
                     /label=Eton CMV-Forward
     primer_bind     286..306
                     /standard_name="Genewiz CMV-Forward"
                     /label=Genewiz CMV-Forward
     primer_bind     293..314
                     /standard_name="QB0976 pRLuc-MCS-F"
                     /label=QB0976 pRLuc-MCS-F
     primer_bind     complement(331..360)
                     /standard_name="352_CMV_Rev2"
                     /label=352_CMV_Rev2
     primer_bind     377..396
                     /standard_name="T7 promoter"
                     /label=T7 promoter
     primer_bind     377..396
                     /standard_name="QB0106 T7"
                     /label=QB0106 T7
     primer_bind     377..396
                     /standard_name="Eton T7 Promoter"
                     /label=Eton T7 Promoter
     primer_bind     377..396
                     /standard_name="Genewiz T7"
                     /label=Genewiz T7
     primer_bind     377..396
                     /standard_name="T7"
                     /label=T7
     promoter        377..395
                     /note="Geneious type: Promoter"
     primer_bind     complement(382..408)
                     /standard_name="197_pCMV_bb_F"
                     /label=197_pCMV_bb_F
     primer_bind     complement(385..408)
                     /standard_name="203_bb_NpuC_R"
                     /label=203_bb_NpuC_R
     primer_bind     complement(389..447)
                     /standard_name="875_Genscript_APOBEC-bpnls-rev"
                     /label=875_Genscript_APOBEC-bpnls-rev
     primer_bind     389..428
                     /standard_name="903_GS_NpuN-fwd"
                     /label=903_GS_NpuN-fwd
     primer_bind     complement(389..408)
                     /standard_name="122_CMV_rev"
                     /label=122_CMV_rev
     primer_bind     complement(389..408)
                     /standard_name="CMV_rev"
                     /label=CMV_rev
     misc_feature    406..408
                     /standard_name="USER1"
                     /label=USER1
     CDS             409..465
                     /standard_name="BPNLS"
                     /label=BPNLS
     misc_feature    complement(445..473)
                     /standard_name="KZ1434"
                     /label=KZ1434
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     462..487
                     /standard_name="KZ1433"
                     /label=KZ1433
     misc_feature    462..473
                     /standard_name="USER"
                     /label=USER
                     /note="Geneious type: overhang"
     CDS             466..963
                     /standard_name="TadAmax10 (ABE8)"
                     /label=TadAmax10 (ABE8)
     polyA_site      617..641
     misc_feature    complement(945..964)
                     /standard_name="KZ1428"
                     /label=KZ1428
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     958..983
                     /standard_name="KZ1427"
                     /label=KZ1427
     misc_feature    964..1059
                     /standard_name="3xGGS-XTEN-3xGGS"
                     /label=3xGGS-XTEN-3xGGS
                     /note="Geneious type: Misc"
     primer_bind     complement(1042..1061)
                     /standard_name="EE 134"
                     /label=EE 134
     primer_bind     1048..1083
                     /standard_name="EE 133"
                     /label=EE 133
     misc_feature    1048..1061
                     /standard_name="USER"
                     /label=USER
                     /note="Geneious type: overhang"
     CDS             1060..4980
                     /codon_start=1
                     /standard_name="AsCpf1"
                     /product="CRISPR-associated protein from Acidaminococcus
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLARGWDVNREKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIARGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQDWLAYIQELRN"
     misc_feature    1060..4978
                     /standard_name="GACAAGAAGTACAGCATCGGCCTGGCCATCGGCACCAACTCT 
                     GTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGCAAGAAATTCAAGGTGC 
                     TGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGAGCCCTGCTGTTCGA 
                     CAGCGGCGAAACAGCCGAGGCCACCCGGCTGAAGAGAACCGCCAGAAGAAGATACACC 
                     AGACGGAAGAACCGGATCTGCTATCTGCAAGAGATCTTCAGCAACGAGATGGCCAAGG 
                     TGGACGACAGCTTCTTCCACAGACTGGAAGAGTCCTTCCTGGTGGAAGAGGATAAGAA 
                     GCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAAG 
                     TACCCCACCATCTACCACCTGAGAAAGAAACTGGTGGACAGCACCGACAAGGCCGACC 
                     TGCGGCTGATCTATCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGAT 
                     CGAGGGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTG 
                     CAGACCTACAACCAGCTGTTCGAGGAAAACCCCATCAACGCCAGCGGCGTGGACGCCA 
                     AGGCCATCCTGTCTGCCAGACTGAGCAAGAGCAGACGGCTGGAAAATCTGATCGCCCA 
                     GCTGCCCGGCGAGAAGAAGAATGGCCTGTTCGGAAACCTGATTGCCCTGAGCCTGGGC 
                     CTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGATGCCAAACTGCAGCTGA 
                     GCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTA 
                     CGCCGACCTGTTTCTGGCCGCCAAGAACCTGTCCGACGCCATCCTGCTGAGCGACATC 
                     CTGAGAGTGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCTCTATGATCAAGAGAT 
                     ACGACGAGCACCACCAGGACCTGACCCTGCTGAAAGCTCTCGTGCGGCAGCAGCTGCC 
                     TGAGAAGTACAAAGAGATTTTCTTCGACCAGAGCAAGAACGGCTACGCCGGCTACATT 
                     GACGGCGGAGCCAGCCAGGAAGAGTTCTACAAGTTCATCAAGCCCATCCTGGAAAAGA 
                     TGGACGGCACCGAGGAACTGCTCGTGAAGCTGAACAGAGAGGACCTGCTGCGGAAGCA 
                     GCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGGGAGAGCTGCACGCC 
                     ATTCTGCGGCGGCAGGAAGATTTTTACCCATTCCTGAAGGACAACCGGGAAAAGATCG 
                     AGAAGATCCTGACCTTCCGCATCCCCTACTACGTGGGCCCTCTGGCCAGGGGAAACAG 
                     CAGATTCGCCTGGATGACCAGAAAGAGCGAGGAAACCATCACCCCCTGGAACTTCGAG 
                     GAAGTGGTGGACAAGGGCGCTTCCGCCCAGAGCTTCATCGAGCGGATGACCAACTTCG 
                     ATAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTT 
                     CACCGTGTATAACGAGCTGACCAAAGTGAAATACGTGACCGAGGGAATGAGAAAGCCC 
                     GCCTTCCTGAGCGGCGAGCAGAAAAAGGCCATCGTGGACCTGCTGTTCAAGACCAACC 
                     GGAAAGTGACCGTGAAGCAGCTGAAAGAGGACTACTTCAAGAAAATCGAGTGCTTCGA 
                     CTCCGTGGAAATCTCCGGCGTGGAAGATCGGTTCAACGCCTCCCTGGGCACATACCAC 
                     GATCTGCTGAAAATTATCAAGGACAAGGACTTCCTGGACAATGAGGAAAACGAGGACA 
                     TTCTGGAAGATATCGTGCTGACCCTGACACTGTTTGAGGACAGAGAGATGATCGAGGA 
                     ACGGCTGAAAACCTATGCCCACCTGTTCGACGACAAAGTGATGAAGCAGCTGAAGCGG 
                     CGGAGATACACCGGCTGGGGCAGGCTGAGCCGGAAGCTGATCAACGGCATCCGGGACA 
                     AGCAGTCCGGCAAGACAATCCTGGATTTCCTGAAGTCCGACGGCTTCGCCAACAGAAA 
                     CTTCATGCAGCTGATCCACGACGACAGCCTGACCTTTAAAGAGGACATCCAGAAAGCC 
                     CAGGTGTCCGGCCAGGGCGATAGCCTGCACGAGCACATTGCCAATCTGGCCGGCAGCC 
                     CCGCCATTAAGAAGGGCATCCTGCAGACAGTGAAGGTGGTGGACGAGCTCGTGAAAGT 
                     GATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAAATGGCCAGAGAGAACCAGACC 
                     ACCCAGAAGGGACAGAAGAACAGCCGCGAGAGAATGAAGCGGATCGAAGAGGGCATCA 
                     AAGAGCTGGGCAGCCAGATCCTGAAAGAACACCCCGTGGAAAACACCCAGCTGCAGAA 
                     CGAGAAGCTGTACCTGTACTACCTGCAGAATGGGCGGGATATGTACGTGGACCAGGAA 
                     CTGGACATCAACCGGCTGTCCGACTACGATGTGGACCATATCGTGCCTCAGAGCTTTC 
                     TGAAGGACGACTCCATCGACAACAAGGTGCTGACCAGAAGCGACAAGAACCGGGGCAA 
                     GAGCGACAACGTGCCCTCCGAAGAGGTCGTGAAGAAGATGAAGAACTACTGGCGGCAG 
                     CTGCTGAACGCCAAGCTGATTACCCAGAGAAAGTTCGACAATCTGACCAAGGCCGAGA 
                     GAGGCGGCCTGAGCGAACTGGATAAGGCCGGCTTCATCAAGAGACAGCTGGTGGAAAC 
                     CCGGCAGATCACAAAGCACGTGGCACAGATCCTGGACTCCCGGATGAACACTAAGTAC 
                     GACGAGAATGACAAGCTGATCCGGGAAGTGAAAGTGATCACCCTGAAGTCCAAGCTGG 
                     TGTCCGATTTCCGGAAGGATTTCCAGTTTTACAAAGTGCGCGAGATCAACAACTACCA 
                     CCACGCCCACGACGCCTACCTGAACGCCGTCGTGGGAACCGCCCTGATCAAAAAGTAC 
                     CCTAAGCTGGAAAGCGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGA 
                     TGATCGCCAAGAGCGAGCAGGAAATCGGCAAGGCTACCGCCAAGTACTTCTTCTACAG 
                     CAACATCATGAACTTTTTCAAGACCGAGATTACCCTGGCCAACGGCGAGATCCGGAAG 
                     CGGCCTCTGATCGAGACAAACGGCGAAACCGGGGAGATCGTGTGGGATAAGGGCCGGG 
                     ATTTTGCCACCGTGCGGAAAGTGCTGAGCATGCCCCAAGTGAATATCGTGAAAAAGAC 
                     CGAGGTGCAGACAGGCGGCTTCAGCAAAGAGTCTATCCTGCCCAAGAGGAACAGCGAT 
                     AAGCTGATCGCCAGAAAGAAGGACTGGGACCCTAAGAAGTACGGCGGCTTCGACAGCC 
                     CCACCGTGGCCTATTCTGTGCTGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCAAGAA 
                     ACTGAAGAGTGTGAAAGAGCTGCTGGGGATCACCATCATGGAAAGAAGCAGCTTCGAG 
                     AAGAATCCCATCGACTTTCTGGAAGCCAAGGGCTACAAAGAAGTGAAAAAGGACCTGA 
                     TCATCAAGCTGCCTAAGTACTCCCTGTTCGAGCTGGAAAACGGCCGGAAGAGAATGCT 
                     GGCCTCTGCCGGCGAACTGCAGAAGGGAAACGAACTGGCCCTGCCCTCCAAATATGTG 
                     AACTTCCTGTACCTGGCCAGCCACTATGAGAAGCTGAAGGGCTCCCCCGAGGATAATG 
                     AGCAGAAACAGCTGTTTGTGGAACAGCACAAGCACTACCTGGACGAGATCATCGAGCA 
                     GATCAGCGAGTTCTCCAAGAGAGTGATCCTGGCCGACGCTAATCTGGACAAAGTGCTG 
                     TCCGCCTACAACAAGCACCGGGATAAGCCCATCAGAGAGCAGGCCGAGAATATCATCC 
                     ACCTGTTTACCCTGACCAATCTGGGAGCCCCTGCCGCCTTCAAGTACTTTGACACCAC 
                     CATCGACCGGAAGAGGTACACCAGCACCAAAGAGGTGCTGGACGCCACCCTGATCCAC 
                     CAGAGCATCACCGGCCTGTACGAGACACGGATCGACCTGTCTCAGCTGGGAGGTG"
                     /label=Geneious type: Editing History Replacement
                     /note="Geneious type: Editing History Replacement"
     polyA_site      1102..1126
     polyA_site      complement(1172..1196)
     polyA_site      1547..1571
     polyA_site      2021..2045
     polyA_site      2504..2528
     polyA_site      2522..2546
     polyA_site      2971..2995
     polyA_site      3472..3496
     primer_bind     complement(3771..3789)
                     /standard_name="EE 218"
                     /label=EE 218
     primer_bind     3778..3801
                     /standard_name="EE 217"
                     /label=EE 217
     misc_feature    3778..3789
                     /standard_name="USER"
                     /label=USER
                     /note="Geneious type: overhang"
     misc_feature    3781..3783
                     /standard_name="D908A"
                     /label=D908A
                     /note="Geneious type: Mutation"
     misc_feature    3789
                     /standard_name="C->T"
                     /label=C->T
                     /note="Geneious type: Mutation"
     polyA_site      3869..3893
     polyA_site      4364..4388
     regulatory      4700..4709
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
     polyA_site      4894..4918
     primer_bind     complement(4958..4983)
                     /standard_name="EE 136"
                     /label=EE 136
     primer_bind     4970..5003
                     /standard_name="EE 135"
                     /label=EE 135
     misc_feature    4970..4983
                     /standard_name="USER"
                     /label=USER
                     /note="Geneious type: overhang"
     CDS             4993..5043
                     /standard_name="BPNLS"
                     /label=BPNLS
     misc_feature    complement(4997..5018)
                     /standard_name="KZ1407"
                     /label=KZ1407
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     5006..5028
                     /standard_name="KZ1406"
                     /label=KZ1406
     misc_feature    5006..5018
                     /standard_name="USER"
                     /label=USER
                     /note="Geneious type: overhang"
     terminator      5095..5322
                     /note="Geneious type: Terminator"
     primer_bind     5121..5139
                     /standard_name="436_bGH_QPCRF2"
                     /label=436_bGH_QPCRF2
     primer_bind     complement(5244..5268)
                     /standard_name="ACK64 (end)"
                     /label=ACK64 (end)
     misc_feature    complement(5244..5268)
                     /standard_name="ACK64"
                     /label=ACK64
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(5258..5277)
                     /standard_name="438_bGH_QPCRR2"
                     /label=438_bGH_QPCRR2
     primer_bind     complement(5263..5281)
                     /standard_name="439_bGH_QPCRR3"
                     /label=439_bGH_QPCRR3
     primer_bind     complement(5283..5302)
                     /standard_name="437_bGH_QPCRR1"
                     /label=437_bGH_QPCRR1
     primer_bind     complement(5303..5322)
                     /standard_name="920_bGH-BamHI-rev"
                     /label=920_bGH-BamHI-rev
     primer_bind     5381..5407
                     /standard_name="654_CAG_fwd"
                     /label=654_CAG_fwd
     primer_bind     complement(5393..5409)
                     /standard_name="Genewiz M13R"
                     /label=Genewiz M13R
     primer_bind     complement(5393..5409)
                     /standard_name="QB0048 M13 Reverse"
                     /label=QB0048 M13 Reverse
     primer_bind     complement(5393..5409)
                     /standard_name="Eton M13R Reverse"
                     /label=Eton M13R Reverse
     primer_bind     complement(5393..5409)
                     /standard_name="M13-R (-26)"
                     /label=M13-R (-26)
     primer_bind     complement(5406..5429)
                     /standard_name="QB0108 Tac promoter"
                     /label=QB0108 Tac promoter
     primer_bind     complement(5406..5429)
                     /standard_name="M13-R (-46)"
                     /label=M13-R (-46)
     primer_bind     complement(5406..5428)
                     /standard_name="QB0050 M13/pUC Reverse"
                     /label=QB0050 M13/pUC Reverse
     promoter        complement(5442..5471)
                     /note="Geneious type: Promoter"
     primer_bind     complement(5478..5497)
                     /standard_name="836_Lac-F"
                     /label=836_Lac-F
     primer_bind     complement(5478..5497)
                     /standard_name="Lac-F (EtonSeq)"
                     /label=Lac-F (EtonSeq)
     primer_bind     5486..5510
                     /standard_name="ACK21"
                     /label=ACK21
     misc_feature    complement(5486..5510)
                     /standard_name="ACK32"
                     /label=ACK32
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(5624..5641)
                     /standard_name="QB0039 L4440"
                     /label=QB0039 L4440
     primer_bind     complement(5676..5695)
                     /standard_name="VR"
                     /label=VR
     primer_bind     complement(5765..5786)
                     /standard_name="11_pFYF_Seq_Rev"
                     /label=11_pFYF_Seq_Rev
     misc_feature    complement(5780..6399)
                     /standard_name="pBR322 origin"
                     /label=pBR322 origin
                     /note="Geneious type: Origin of Replication"
     primer_bind     5795..5819
                     /standard_name="ACK10"
                     /label=ACK10
     primer_bind     complement(5856..5875)
                     /standard_name="BR322-F"
                     /label=BR322-F
     misc_feature    5991
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
     primer_bind     6018..6036
                     /standard_name="QB0766 RepO-F"
                     /label=QB0766 RepO-F
     primer_bind     6115..6134
                     /standard_name="833_BR322-rev1"
                     /label=833_BR322-rev1
     primer_bind     complement(6143..6162)
                     /standard_name="QB0767 RepO-R"
                     /label=QB0767 RepO-R
     misc_feature    6236
                     /standard_name="G"
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     primer_bind     6296..6320
                     /standard_name="ACK11"
                     /label=ACK11
     primer_bind     complement(6296..6315)
                     /standard_name="832_BR322-fwd1"
                     /label=832_BR322-fwd1
     misc_feature    complement(6554..7414)
                     /standard_name="amp marker"
                     /label=amp marker
                     /note="Geneious type: Marker"
     primer_bind     6795..6819
                     /standard_name="ACK12"
                     /label=ACK12
     primer_bind     6960..6979
                     /standard_name="ACK563"
                     /label=ACK563
     primer_bind     7177..7196
                     /standard_name="QB0005 Amp-R"
                     /label=QB0005 Amp-R
     primer_bind     complement(7262..7289)
                     /standard_name="562_Amp_Fwd"
                     /label=562_Amp_Fwd
     primer_bind     7262..7289
                     /standard_name="563_Amp_Rev"
                     /label=563_Amp_Rev
     primer_bind     7295..7319
                     /standard_name="ACK13"
                     /label=ACK13
     promoter        complement(7456..7484)
                     /note="Geneious type: Promoter"
     primer_bind     7528..7545
                     /standard_name="QB0090 pQE promoter"
                     /label=QB0090 pQE promoter
     primer_bind     complement(7598..7617)
                     /standard_name="QB0092 pRS-marker"
                     /label=QB0092 pRS-marker
     primer_bind     7798..7822
                     /standard_name="JLseq006_ACK14"
                     /label=JLseq006_ACK14
     primer_bind     7798..7822
                     /standard_name="ACK14"
                     /label=ACK14
     promoter        join(7805..8037,1..356)
     primer_bind     7805..7848
                     /standard_name="387_pAAV_fwd"
                     /label=387_pAAV_fwd
     primer_bind     complement(7855..7874)
                     /standard_name="QB1110 pSicoR"
                     /label=QB1110 pSicoR
     misc_feature    7994
                     /standard_name="C"
                     /label=C
                     /note="Geneious type: Editing History Replacement"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctctga ggtggagttt
      481 tcccacgagt actggatgag acatgccctg accctggcca agagggcacg ggatgagagg
      541 gaggtgcctg tgggagccgt gctggtgctg aacaatagag tgatcggcga gggctggaac
      601 agagccatcg gcctgcacga cccaacagcc catgccgaaa ttatggccct gagacagggc
      661 ggcctggtca tgcagaacta cagactgatt gacgccaccc tgtacgtgac attcgagcct
      721 tgcgtgatgt gcgccggcgc catgatccac tctaggatcg gccgcgtggt gtttggcgtg
      781 aggaactcaa aaagaggcgc cgcaggctcc ctgatgaacg tgctgaacta ccccggcatg
      841 aatcaccgcg tcgaaattac cgagggaatc ctggcagatg aatgtgccgc cctgctgtgc
      901 gatttctatc ggatgcctag acaggtgttc aatgctcaga agaaggccca gagctccatc
      961 aactccggag gatctagcgg aggctcctct ggctctgaga cacctggcac aagcgagagc
     1021 gcaacacctg aaagcagcgg gggcagcagc ggggggtcaa tgacacagtt cgagggcttt
     1081 accaacctgt atcaggtgag caagacactg cggtttgagc tgatcccaca gggcaagacc
     1141 ctgaagcaca tccaggagca gggcttcatc gaggaggaca aggcccgcaa tgatcactac
     1201 aaggagctga agcccatcat cgatcggatc tacaagacct atgccgacca gtgcctgcag
     1261 ctggtgcagc tggattggga gaacctgagc gccgccatcg actcctatag aaaggagaaa
     1321 accgaggaga caaggaacgc cctgatcgag gagcaggcca catatcgcaa tgccatccac
     1381 gactacttca tcggccggac agacaacctg accgatgcca tcaataagag acacgccgag
     1441 atctacaagg gcctgttcaa ggccgagctg tttaatggca aggtgctgaa gcagctgggc
     1501 accgtgacca caaccgagca cgagaacgcc ctgctgcgga gcttcgacaa gtttacaacc
     1561 tacttctccg gcttttatag aaacaggaag aacgtgttca gcgccgagga tatcagcaca
     1621 gccatcccac accgcatcgt gcaggacaac ttccccaagt ttaaggagaa ttgtcacatc
     1681 ttcacacgcc tgatcaccgc cgtgcccagc ctgcgggagc actttgagaa cgtgaagaag
     1741 gccatcggca tcttcgtgag cacctccatc gaggaggtgt tttccttccc tttttataac
     1801 cagctgctga cacagaccca gatcgacctg tataaccagc tgctgggagg aatctctcgg
     1861 gaggcaggca ccgagaagat caagggcctg aacgaggtgc tgaatctggc catccagaag
     1921 aatgatgaga cagcccacat catcgcctcc ctgccacaca gattcatccc cctgtttaag
     1981 cagatcctgt ccgataggaa caccctgtct ttcatcctgg aggagtttaa gagcgacgag
     2041 gaagtgatcc agtccttctg caagtacaag acactgctga gaaacgagaa cgtgctggag
     2101 acagccgagg ccctgtttaa cgagctgaac agcatcgacc tgacacacat cttcatcagc
     2161 cacaagaagc tggagacaat cagcagcgcc ctgtgcgacc actgggatac actgaggaat
     2221 gccctgtatg agcggagaat ctccgagctg acaggcaaga tcaccaagtc tgccaaggag
     2281 aaggtgcagc gcagcctgaa gcacgaggat atcaacctgc aggagatcat ctctgccgca
     2341 ggcaaggagc tgagcgaggc cttcaagcag aaaaccagcg agatcctgtc ccacgcacac
     2401 gccgccctgg atcagccact gcctacaacc ctgaagaagc aggaggagaa ggagatcctg
     2461 aagtctcagc tggacagcct gctgggcctg taccacctgc tggactggtt tgccgtggat
     2521 gagtccaacg aggtggaccc cgagttctct gcccggctga ccggcatcaa gctggagatg
     2581 gagccttctc tgagcttcta caacaaggcc agaaattatg ccaccaagaa gccctactcc
     2641 gtggagaagt tcaagctgaa ctttcagatg cctacactgg ccagaggctg ggacgtgaat
     2701 agagagaaga acaatggcgc catcctgttt gtgaagaacg gcctgtacta tctgggcatc
     2761 atgccaaagc agaagggcag gtataaggcc ctgagcttcg agcccacaga gaaaaccagc
     2821 gagggctttg ataagatgta ctatgactac ttccctgatg ccgccaagat gatcccaaag
     2881 tgcagcaccc agctgaaggc cgtgacagcc cactttcaga cccacacaac ccccatcctg
     2941 ctgtccaaca atttcatcga gcctctggag atcacaaagg agatctacga cctgaacaat
     3001 cctgagaagg agccaaagaa gtttcagaca gcctacgcca agaaaaccgg cgaccagaag
     3061 ggctacagag aggccctgtg caagtggatc gacttcacaa gggattttct gtccaagtat
     3121 accaagacaa cctctatcga tctgtctagc ctgcggccat cctctcagta taaggacctg
     3181 ggcgagtact atgccgagct gaatcccctg ctgtaccaca tcagcttcca gagaatcgcc
     3241 gagaaggaga tcatggatgc cgtggagaca ggcaagctgt acctgttcca gatctataac
     3301 aaggactttg ccaagggcca ccacggcaag cctaatctgc acacactgta ttggaccggc
     3361 ctgttttctc cagagaacct ggccaagaca agcatcaagc tgaatggcca ggccgagctg
     3421 ttctaccgcc ctaagtccag gatgaagagg atggcacacc ggctgggaga gaagatgctg
     3481 aacaagaagc tgaaggatca gaaaacccca atccccgaca ccctgtacca ggagctgtac
     3541 gactatgtga atcacagact gtcccacgac ctgtctgatg aggccagggc cctgctgccc
     3601 aacgtgatca ccaaggaggt gtctcacgag atcatcaagg ataggcgctt taccagcgac
     3661 aagttctttt tccacgtgcc tatcacactg aactatcagg ccgccaattc cccatctaag
     3721 ttcaaccaga gggtgaatgc ctacctgaag gagcaccccg agacacctat catcggcatc
     3781 gcccggggtg agagaaacct gatctatatc acagtgatcg actccaccgg caagatcctg
     3841 gagcagcgga gcctgaacac catccagcag tttgattacc agaagaagct ggacaacagg
     3901 gagaaggaga gggtggcagc aaggcaggcc tggtctgtgg tgggcacaat caaggatctg
     3961 aagcagggct atctgagcca ggtcatccac gagatcgtgg acctgatgat ccactaccag
     4021 gccgtggtgg tgctggagaa cctgaatttc ggctttaaga gcaagaggac cggcatcgcc
     4081 gagaaggccg tgtaccagca gttcgagaag atgctgatcg ataagctgaa ttgcctggtg
     4141 ctgaaggact atccagcaga gaaagtggga ggcgtgctga acccatacca gctgacagac
     4201 cagttcacct cctttgccaa gatgggcacc cagtctggct tcctgtttta cgtgcctgcc
     4261 ccatatacat ctaagatcga tcccctgacc ggcttcgtgg accccttcgt gtggaaaacc
     4321 atcaagaatc acgagagccg caagcacttc ctggagggct tcgactttct gcactacgac
     4381 gtgaaaaccg gcgacttcat cctgcacttt aagatgaaca gaaatctgtc cttccagagg
     4441 ggcctgcccg gctttatgcc tgcatgggat atcgtgttcg agaagaacga gacacagttt
     4501 gacgccaagg gcaccccttt catcgccggc aagagaatcg tgccagtgat cgagaatcac
     4561 agattcaccg gcagataccg ggacctgtat cctgccaacg agctgatcgc cctgctggag
     4621 gagaagggca tcgtgttcag ggatggctcc aacatcctgc caaagctgct ggagaatgac
     4681 gattctcacg ccatcgacac catggtggcc ctgatccgca gcgtgctgca gatgcggaac
     4741 tccaatgccg ccacaggcga ggactatatc aacagccccg tgcgcgatct gaatggcgtg
     4801 tgcttcgact cccggtttca gaacccagag tggcccatgg acgccgatgc caatggcgcc
     4861 taccacatcg ccctgaaggg ccagctgctg ctgaatcacc tgaaggagag caaggatctg
     4921 aagctgcaga acggcatctc caatcaggac tggctggcct acatccagga gctgcgcaac
     4981 tctggcggct caaaaagaac cgccgacggc agcgaattcg agcccaagaa gaagaggaaa
     5041 gtctaaccgg tcatcatcac catcaccatt gagtttaaac ccgctgatca gcctcgactg
     5101 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     5161 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     5221 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     5281 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     5341 ccagctgggg ctcgataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
     5401 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     5461 taaagtgtaa agcctagggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     5521 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     5581 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     5641 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     5701 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     5761 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     5821 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5881 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     5941 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6001 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6061 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6121 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6181 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6241 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6301 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6361 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     6421 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     6481 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     6541 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     6601 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     6661 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     6721 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     6781 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     6841 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     6901 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     6961 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7021 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7081 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7141 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7201 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7261 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7321 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7381 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     7441 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     7501 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     7561 gagatcgatc tcccgatccc ctagggtcga ctctcagtac aatctgctct gatgccgcat
     7621 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     7681 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     7741 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
     7801 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     7861 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     7921 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     7981 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatc
//
