LOCUS Exported 8631 bp ds-DNA circular UNA 31-DEC-2019 DEFINITION ABEmax10. ACCESSION urn.local...c-8wnwdza VERSION . KEYWORDS . SOURCE natural DNA sequence ORGANISM unspecified REFERENCE 1 (bases 1 to 8631) AUTHORS . TITLE Direct Submission JOURNAL Exported Thursday, Jan 2, 2020 from SnapGene Viewer 5.0.7 https://www.snapgene.com FEATURES Location/Qualifiers source 1..8631 /organism="unspecified" /mol_type="genomic DNA" source 1654..5544 /organism="synthetic DNA construct" /mol_type="other DNA" source 5545..5574 /organism="synthetic DNA construct" /mol_type="other DNA" source 5575..5586 primer_bind 234..253 /standard_name="LCv2_seq1" /label=LCv2_seq1 primer_bind 261..285 /standard_name="JLseq007_ACK15" /label=JLseq007_ACK15 primer_bind 261..285 /standard_name="ACK15" /label=ACK15 promoter 275..356 /note="Geneious type: Promoter" primer_bind 286..306 /standard_name="QB0012 CMV Forward" /label=QB0012 CMV Forward primer_bind 286..306 /standard_name="Eton CMV-Forward" /label=Eton CMV-Forward primer_bind 286..306 /standard_name="Genewiz CMV-Forward" /label=Genewiz CMV-Forward primer_bind 293..314 /standard_name="QB0976 pRLuc-MCS-F" /label=QB0976 pRLuc-MCS-F primer_bind complement(331..360) /standard_name="352_CMV_Rev2" /label=352_CMV_Rev2 primer_bind 377..396 /standard_name="T7 promoter" /label=T7 promoter primer_bind 377..396 /standard_name="QB0106 T7" /label=QB0106 T7 primer_bind 377..396 /standard_name="Eton T7 Promoter" /label=Eton T7 Promoter primer_bind 377..396 /standard_name="Genewiz T7" /label=Genewiz T7 primer_bind 377..396 /standard_name="T7" /label=T7 promoter 377..395 /note="Geneious type: Promoter" primer_bind complement(382..408) /standard_name="197_pCMV_bb_F" /label=197_pCMV_bb_F primer_bind complement(385..408) /standard_name="203_bb_NpuC_R" /label=203_bb_NpuC_R primer_bind complement(389..447) /standard_name="875_Genscript_APOBEC-bpnls-rev" /label=875_Genscript_APOBEC-bpnls-rev primer_bind 389..428 /standard_name="903_GS_NpuN-fwd" /label=903_GS_NpuN-fwd primer_bind complement(389..408) /standard_name="122_CMV_rev" /label=122_CMV_rev primer_bind complement(389..408) /standard_name="CMV_rev" /label=CMV_rev misc_feature 406..408 /standard_name="USER1" /label=USER1 CDS 409..465 /standard_name="BPNLS" /label=BPNLS primer_bind 428..487 /standard_name="925_Genscript_ABE-BPNLS-fwd" /label=925_Genscript_ABE-BPNLS-fwd CDS 466..1059 /standard_name="ABE 6.20" /label=ABE 6.20 primer_bind 690..714 /standard_name="KZ918" /label=KZ918 misc_feature complement(690..714) /standard_name="KZ922" /label=KZ922 /note="Geneious type: primer_bind_reverse" misc_feature complement(1042..1062) /standard_name="KZ1424" /label=KZ1424 /note="Geneious type: primer_bind_reverse" primer_bind 1053..1083 /standard_name="KZ1423" /label=KZ1423 CDS 1060..1557 /standard_name="max10" /label=max10 misc_feature complement(1539..1558) /standard_name="KZ1428" /label=KZ1428 /note="Geneious type: primer_bind_reverse" primer_bind 1552..1577 /standard_name="KZ1427" /label=KZ1427 misc_feature 1558..1653 /standard_name="3xGGS-XTEN-3xGGS" /label=3xGGS-XTEN-3xGGS /note="Geneious type: Misc" primer_bind complement(1636..1655) /standard_name="EE 134" /label=EE 134 primer_bind 1642..1677 /standard_name="EE 133" /label=EE 133 misc_feature 1642..1655 /standard_name="USER" /label=USER /note="Geneious type: overhang" CDS 1654..5544 /codon_start=1 /standard_name="AsCpf1" /product="CRISPR-associated protein from Acidaminococcus sp. BV3L6 (Zetsche et al., 2015)" /label=AsCpf1 /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD KFTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA RNYATKKPYSVEKFKLNFQMPTLARGWDVNREKNNGAILFVKNGLYYLGIMPKQKGRYK ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIARGERNLIYITVIDSTGKILEQR SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES KDLKLQNGISNQD" misc_feature 1654..5543 /standard_name="GACAAGAAGTACAGCATCGGCCTGGCCATCGGCACCAACTCT GTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGCAAGAAATTCAAGGTGC TGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGAGCCCTGCTGTTCGA CAGCGGCGAAACAGCCGAGGCCACCCGGCTGAAGAGAACCGCCAGAAGAAGATACACC AGACGGAAGAACCGGATCTGCTATCTGCAAGAGATCTTCAGCAACGAGATGGCCAAGG TGGACGACAGCTTCTTCCACAGACTGGAAGAGTCCTTCCTGGTGGAAGAGGATAAGAA GCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAAG TACCCCACCATCTACCACCTGAGAAAGAAACTGGTGGACAGCACCGACAAGGCCGACC TGCGGCTGATCTATCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGAT CGAGGGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTG CAGACCTACAACCAGCTGTTCGAGGAAAACCCCATCAACGCCAGCGGCGTGGACGCCA AGGCCATCCTGTCTGCCAGACTGAGCAAGAGCAGACGGCTGGAAAATCTGATCGCCCA GCTGCCCGGCGAGAAGAAGAATGGCCTGTTCGGAAACCTGATTGCCCTGAGCCTGGGC CTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGATGCCAAACTGCAGCTGA GCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTA CGCCGACCTGTTTCTGGCCGCCAAGAACCTGTCCGACGCCATCCTGCTGAGCGACATC CTGAGAGTGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCTCTATGATCAAGAGAT ACGACGAGCACCACCAGGACCTGACCCTGCTGAAAGCTCTCGTGCGGCAGCAGCTGCC TGAGAAGTACAAAGAGATTTTCTTCGACCAGAGCAAGAACGGCTACGCCGGCTACATT GACGGCGGAGCCAGCCAGGAAGAGTTCTACAAGTTCATCAAGCCCATCCTGGAAAAGA TGGACGGCACCGAGGAACTGCTCGTGAAGCTGAACAGAGAGGACCTGCTGCGGAAGCA GCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGGGAGAGCTGCACGCC ATTCTGCGGCGGCAGGAAGATTTTTACCCATTCCTGAAGGACAACCGGGAAAAGATCG AGAAGATCCTGACCTTCCGCATCCCCTACTACGTGGGCCCTCTGGCCAGGGGAAACAG CAGATTCGCCTGGATGACCAGAAAGAGCGAGGAAACCATCACCCCCTGGAACTTCGAG GAAGTGGTGGACAAGGGCGCTTCCGCCCAGAGCTTCATCGAGCGGATGACCAACTTCG ATAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTT CACCGTGTATAACGAGCTGACCAAAGTGAAATACGTGACCGAGGGAATGAGAAAGCCC GCCTTCCTGAGCGGCGAGCAGAAAAAGGCCATCGTGGACCTGCTGTTCAAGACCAACC GGAAAGTGACCGTGAAGCAGCTGAAAGAGGACTACTTCAAGAAAATCGAGTGCTTCGA CTCCGTGGAAATCTCCGGCGTGGAAGATCGGTTCAACGCCTCCCTGGGCACATACCAC GATCTGCTGAAAATTATCAAGGACAAGGACTTCCTGGACAATGAGGAAAACGAGGACA TTCTGGAAGATATCGTGCTGACCCTGACACTGTTTGAGGACAGAGAGATGATCGAGGA ACGGCTGAAAACCTATGCCCACCTGTTCGACGACAAAGTGATGAAGCAGCTGAAGCGG CGGAGATACACCGGCTGGGGCAGGCTGAGCCGGAAGCTGATCAACGGCATCCGGGACA AGCAGTCCGGCAAGACAATCCTGGATTTCCTGAAGTCCGACGGCTTCGCCAACAGAAA CTTCATGCAGCTGATCCACGACGACAGCCTGACCTTTAAAGAGGACATCCAGAAAGCC CAGGTGTCCGGCCAGGGCGATAGCCTGCACGAGCACATTGCCAATCTGGCCGGCAGCC CCGCCATTAAGAAGGGCATCCTGCAGACAGTGAAGGTGGTGGACGAGCTCGTGAAAGT GATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAAATGGCCAGAGAGAACCAGACC ACCCAGAAGGGACAGAAGAACAGCCGCGAGAGAATGAAGCGGATCGAAGAGGGCATCA AAGAGCTGGGCAGCCAGATCCTGAAAGAACACCCCGTGGAAAACACCCAGCTGCAGAA CGAGAAGCTGTACCTGTACTACCTGCAGAATGGGCGGGATATGTACGTGGACCAGGAA CTGGACATCAACCGGCTGTCCGACTACGATGTGGACCATATCGTGCCTCAGAGCTTTC TGAAGGACGACTCCATCGACAACAAGGTGCTGACCAGAAGCGACAAGAACCGGGGCAA GAGCGACAACGTGCCCTCCGAAGAGGTCGTGAAGAAGATGAAGAACTACTGGCGGCAG CTGCTGAACGCCAAGCTGATTACCCAGAGAAAGTTCGACAATCTGACCAAGGCCGAGA GAGGCGGCCTGAGCGAACTGGATAAGGCCGGCTTCATCAAGAGACAGCTGGTGGAAAC CCGGCAGATCACAAAGCACGTGGCACAGATCCTGGACTCCCGGATGAACACTAAGTAC GACGAGAATGACAAGCTGATCCGGGAAGTGAAAGTGATCACCCTGAAGTCCAAGCTGG TGTCCGATTTCCGGAAGGATTTCCAGTTTTACAAAGTGCGCGAGATCAACAACTACCA CCACGCCCACGACGCCTACCTGAACGCCGTCGTGGGAACCGCCCTGATCAAAAAGTAC CCTAAGCTGGAAAGCGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGA TGATCGCCAAGAGCGAGCAGGAAATCGGCAAGGCTACCGCCAAGTACTTCTTCTACAG CAACATCATGAACTTTTTCAAGACCGAGATTACCCTGGCCAACGGCGAGATCCGGAAG CGGCCTCTGATCGAGACAAACGGCGAAACCGGGGAGATCGTGTGGGATAAGGGCCGGG ATTTTGCCACCGTGCGGAAAGTGCTGAGCATGCCCCAAGTGAATATCGTGAAAAAGAC CGAGGTGCAGACAGGCGGCTTCAGCAAAGAGTCTATCCTGCCCAAGAGGAACAGCGAT AAGCTGATCGCCAGAAAGAAGGACTGGGACCCTAAGAAGTACGGCGGCTTCGACAGCC CCACCGTGGCCTATTCTGTGCTGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCAAGAA ACTGAAGAGTGTGAAAGAGCTGCTGGGGATCACCATCATGGAAAGAAGCAGCTTCGAG AAGAATCCCATCGACTTTCTGGAAGCCAAGGGCTACAAAGAAGTGAAAAAGGACCTGA TCATCAAGCTGCCTAAGTACTCCCTGTTCGAGCTGGAAAACGGCCGGAAGAGAATGCT GGCCTCTGCCGGCGAACTGCAGAAGGGAAACGAACTGGCCCTGCCCTCCAAATATGTG AACTTCCTGTACCTGGCCAGCCACTATGAGAAGCTGAAGGGCTCCCCCGAGGATAATG AGCAGAAACAGCTGTTTGTGGAACAGCACAAGCACTACCTGGACGAGATCATCGAGCA GATCAGCGAGTTCTCCAAGAGAGTGATCCTGGCCGACGCTAATCTGGACAAAGTGCTG TCCGCCTACAACAAGCACCGGGATAAGCCCATCAGAGAGCAGGCCGAGAATATCATCC ACCTGTTTACCCTGACCAATCTGGGAGCCCCTGCCGCCTTCAAGTACTTTGACACCAC CATCGACCGGAAGAGGTACACCAGCACCAAAGAGGTGCTGGACGCCACCCTGATCCAC CAGAGCATCACCGGCCTGTACGAGACACGGATCGACCTGTCTCAGCTGGGAGGTGACT CTGGCGGCTCAAAAAGAACCGC" /label=Geneious type: Editing History Replacement /note="Geneious type: Editing History Replacement" primer_bind complement(4365..4383) /standard_name="EE 218" /label=EE 218 primer_bind 4372..4395 /standard_name="EE 217" /label=EE 217 misc_feature 4372..4383 /standard_name="USER" /label=USER /note="Geneious type: overhang" regulatory 5294..5303 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" misc_feature 5545..5598 /label=Geneious type: Editing History Insertion /note="Geneious type: Editing History Insertion" CDS 5545..5574 /codon_start=1 /standard_name="AsCpf1" /product="CRISPR-associated protein from Acidaminococcus sp. BV3L6 (Zetsche et al., 2015)" /label=AsCpf1 /translation="MLAYIQELRN" primer_bind complement(5552..5577) /standard_name="EE 136" /label=EE 136 primer_bind 5564..5597 /standard_name="EE 135" /label=EE 135 misc_feature 5564..5577 /standard_name="USER" /label=USER /note="Geneious type: overhang" CDS 5587..5598 /standard_name="BPNLS" /label=BPNLS CDS 5599..5637 /standard_name="BPNLS" /label=BPNLS primer_bind 5600..5622 /standard_name="KZ1406" /label=KZ1406 misc_feature 5600..5612 /standard_name="USER" /label=USER /note="Geneious type: overhang" terminator 5689..5916 /note="Geneious type: Terminator" primer_bind 5715..5733 /standard_name="436_bGH_QPCRF2" /label=436_bGH_QPCRF2 primer_bind complement(5838..5862) /standard_name="ACK64 (end)" /label=ACK64 (end) misc_feature complement(5838..5862) /standard_name="ACK64" /label=ACK64 /note="Geneious type: primer_bind_reverse" primer_bind complement(5852..5871) /standard_name="438_bGH_QPCRR2" /label=438_bGH_QPCRR2 primer_bind complement(5857..5875) /standard_name="439_bGH_QPCRR3" /label=439_bGH_QPCRR3 primer_bind complement(5877..5896) /standard_name="437_bGH_QPCRR1" /label=437_bGH_QPCRR1 primer_bind complement(5897..5916) /standard_name="920_bGH-BamHI-rev" /label=920_bGH-BamHI-rev primer_bind 5975..6001 /standard_name="654_CAG_fwd" /label=654_CAG_fwd primer_bind complement(5987..6003) /standard_name="Genewiz M13R" /label=Genewiz M13R primer_bind complement(5987..6003) /standard_name="QB0048 M13 Reverse" /label=QB0048 M13 Reverse primer_bind complement(5987..6003) /standard_name="Eton M13R Reverse" /label=Eton M13R Reverse primer_bind complement(5987..6003) /standard_name="M13-R (-26)" /label=M13-R (-26) primer_bind complement(6000..6023) /standard_name="QB0108 Tac promoter" /label=QB0108 Tac promoter primer_bind complement(6000..6023) /standard_name="M13-R (-46)" /label=M13-R (-46) primer_bind complement(6000..6022) /standard_name="QB0050 M13/pUC Reverse" /label=QB0050 M13/pUC Reverse promoter complement(6036..6065) /note="Geneious type: Promoter" primer_bind complement(6072..6091) /standard_name="836_Lac-F" /label=836_Lac-F primer_bind complement(6072..6091) /standard_name="Lac-F (EtonSeq)" /label=Lac-F (EtonSeq) primer_bind 6080..6104 /standard_name="ACK21" /label=ACK21 misc_feature complement(6080..6104) /standard_name="ACK32" /label=ACK32 /note="Geneious type: primer_bind_reverse" primer_bind complement(6218..6235) /standard_name="QB0039 L4440" /label=QB0039 L4440 primer_bind complement(6270..6289) /standard_name="VR" /label=VR primer_bind complement(6359..6380) /standard_name="11_pFYF_Seq_Rev" /label=11_pFYF_Seq_Rev misc_feature complement(6374..6993) /standard_name="pBR322 origin" /label=pBR322 origin /note="Geneious type: Origin of Replication" primer_bind 6389..6413 /standard_name="ACK10" /label=ACK10 primer_bind complement(6450..6469) /standard_name="BR322-F" /label=BR322-F misc_feature 6585 /label=Geneious type: Editing History Insertion /note="Geneious type: Editing History Insertion" primer_bind 6612..6630 /standard_name="QB0766 RepO-F" /label=QB0766 RepO-F primer_bind 6709..6728 /standard_name="833_BR322-rev1" /label=833_BR322-rev1 primer_bind complement(6737..6756) /standard_name="QB0767 RepO-R" /label=QB0767 RepO-R misc_feature 6830 /standard_name="G" /label=G /note="Geneious type: Editing History Replacement" primer_bind 6890..6914 /standard_name="ACK11" /label=ACK11 primer_bind complement(6890..6909) /standard_name="832_BR322-fwd1" /label=832_BR322-fwd1 misc_feature complement(7148..8008) /standard_name="amp marker" /label=amp marker /note="Geneious type: Marker" primer_bind 7389..7413 /standard_name="ACK12" /label=ACK12 primer_bind 7554..7573 /standard_name="ACK563" /label=ACK563 primer_bind 7771..7790 /standard_name="QB0005 Amp-R" /label=QB0005 Amp-R primer_bind complement(7856..7883) /standard_name="562_Amp_Fwd" /label=562_Amp_Fwd primer_bind 7856..7883 /standard_name="563_Amp_Rev" /label=563_Amp_Rev primer_bind 7889..7913 /standard_name="ACK13" /label=ACK13 promoter complement(8050..8078) /note="Geneious type: Promoter" primer_bind 8122..8139 /standard_name="QB0090 pQE promoter" /label=QB0090 pQE promoter primer_bind complement(8192..8211) /standard_name="QB0092 pRS-marker" /label=QB0092 pRS-marker primer_bind 8392..8416 /standard_name="JLseq006_ACK14" /label=JLseq006_ACK14 primer_bind 8392..8416 /standard_name="ACK14" /label=ACK14 promoter join(8399..8631,1..356) primer_bind 8399..8442 /standard_name="387_pAAV_fwd" /label=387_pAAV_fwd primer_bind complement(8449..8468) /standard_name="QB1110 pSicoR" /label=QB1110 pSicoR misc_feature 8588 /standard_name="C" /label=C /note="Geneious type: Editing History Replacement" ORIGIN 1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctctga agtcgagttt 481 agccacgagt attggatgag gcacgcactg accctggcaa agcgagcatg ggatgaaaga 541 gaagtccccg tgggcgccgt gctggtgcac aacaatagag tgatcggaga gggatggaac 601 aggccaatcg gccgccacga ccctaccgca cacgcagaga tcatggcact gaggcaggga 661 ggcctggtca tgcagaatta ccgcctgatc gatgccaccc tgtatgtgac actggagcca 721 tgcgtgatgt gcgcaggagc aatgatccac agcaggatcg gaagagtggt gttcggagca 781 cgggacgcca agaccggcgc agcaggctcc ctgatggatg tgctgcacca ccccggcatg 841 aaccaccggg tggagatcac agagggaatc ctggcagacg agtgcgccgc cctgctgagc 901 gatttcttta gaatgcggag acaggagatc aaggcccaga agaaggcaca gagctccacc 961 gactctggag gatctagcgg aggatcctct ggaagcgaga caccaggcac aagcgagtcc 1021 gccacaccag agagctccgg cggctcctcc ggaggatcct ctgaggtgga gttttcccac 1081 gagtactgga tgagacatgc cctgaccctg gccaagaggg cacgggatga gagggaggtg 1141 cctgtgggag ccgtgctggt gctgaacaat agagtgatcg gcgagggctg gaacagagcc 1201 atcggcctgc acgacccaac agcccatgcc gaaattatgg ccctgagaca gggcggcctg 1261 gtcatgcaga actacagact gattgacgcc accctgtacg tgacattcga gccttgcgtg 1321 atgtgcgccg gcgccatgat ccactctagg atcggccgcg tggtgtttgg cgtgaggaac 1381 tcaaaaagag gcgccgcagg ctccctgatg aacgtgctga actaccccgg catgaatcac 1441 cgcgtcgaaa ttaccgaggg aatcctggca gatgaatgtg ccgccctgct gtgcgatttc 1501 tatcggatgc ctagacaggt gttcaatgct cagaagaagg cccagagctc catcaactcc 1561 ggaggatcta gcggaggctc ctctggctct gagacacctg gcacaagcga gagcgcaaca 1621 cctgaaagca gcgggggcag cagcgggggg tcaatgacac agttcgaggg ctttaccaac 1681 ctgtatcagg tgagcaagac actgcggttt gagctgatcc cacagggcaa gaccctgaag 1741 cacatccagg agcagggctt catcgaggag gacaaggccc gcaatgatca ctacaaggag 1801 ctgaagccca tcatcgatcg gatctacaag acctatgccg accagtgcct gcagctggtg 1861 cagctggatt gggagaacct gagcgccgcc atcgactcct atagaaagga gaaaaccgag 1921 gagacaagga acgccctgat cgaggagcag gccacatatc gcaatgccat ccacgactac 1981 ttcatcggcc ggacagacaa cctgaccgat gccatcaata agagacacgc cgagatctac 2041 aagggcctgt tcaaggccga gctgtttaat ggcaaggtgc tgaagcagct gggcaccgtg 2101 accacaaccg agcacgagaa cgccctgctg cggagcttcg acaagtttac aacctacttc 2161 tccggctttt atagaaacag gaagaacgtg ttcagcgccg aggatatcag cacagccatc 2221 ccacaccgca tcgtgcagga caacttcccc aagtttaagg agaattgtca catcttcaca 2281 cgcctgatca ccgccgtgcc cagcctgcgg gagcactttg agaacgtgaa gaaggccatc 2341 ggcatcttcg tgagcacctc catcgaggag gtgttttcct tcccttttta taaccagctg 2401 ctgacacaga cccagatcga cctgtataac cagctgctgg gaggaatctc tcgggaggca 2461 ggcaccgaga agatcaaggg cctgaacgag gtgctgaatc tggccatcca gaagaatgat 2521 gagacagccc acatcatcgc ctccctgcca cacagattca tccccctgtt taagcagatc 2581 ctgtccgata ggaacaccct gtctttcatc ctggaggagt ttaagagcga cgaggaagtg 2641 atccagtcct tctgcaagta caagacactg ctgagaaacg agaacgtgct ggagacagcc 2701 gaggccctgt ttaacgagct gaacagcatc gacctgacac acatcttcat cagccacaag 2761 aagctggaga caatcagcag cgccctgtgc gaccactggg atacactgag gaatgccctg 2821 tatgagcgga gaatctccga gctgacaggc aagatcacca agtctgccaa ggagaaggtg 2881 cagcgcagcc tgaagcacga ggatatcaac ctgcaggaga tcatctctgc cgcaggcaag 2941 gagctgagcg aggccttcaa gcagaaaacc agcgagatcc tgtcccacgc acacgccgcc 3001 ctggatcagc cactgcctac aaccctgaag aagcaggagg agaaggagat cctgaagtct 3061 cagctggaca gcctgctggg cctgtaccac ctgctggact ggtttgccgt ggatgagtcc 3121 aacgaggtgg accccgagtt ctctgcccgg ctgaccggca tcaagctgga gatggagcct 3181 tctctgagct tctacaacaa ggccagaaat tatgccacca agaagcccta ctccgtggag 3241 aagttcaagc tgaactttca gatgcctaca ctggccagag gctgggacgt gaatagagag 3301 aagaacaatg gcgccatcct gtttgtgaag aacggcctgt actatctggg catcatgcca 3361 aagcagaagg gcaggtataa ggccctgagc ttcgagccca cagagaaaac cagcgagggc 3421 tttgataaga tgtactatga ctacttccct gatgccgcca agatgatccc aaagtgcagc 3481 acccagctga aggccgtgac agcccacttt cagacccaca caacccccat cctgctgtcc 3541 aacaatttca tcgagcctct ggagatcaca aaggagatct acgacctgaa caatcctgag 3601 aaggagccaa agaagtttca gacagcctac gccaagaaaa ccggcgacca gaagggctac 3661 agagaggccc tgtgcaagtg gatcgacttc acaagggatt ttctgtccaa gtataccaag 3721 acaacctcta tcgatctgtc tagcctgcgg ccatcctctc agtataagga cctgggcgag 3781 tactatgccg agctgaatcc cctgctgtac cacatcagct tccagagaat cgccgagaag 3841 gagatcatgg atgccgtgga gacaggcaag ctgtacctgt tccagatcta taacaaggac 3901 tttgccaagg gccaccacgg caagcctaat ctgcacacac tgtattggac cggcctgttt 3961 tctccagaga acctggccaa gacaagcatc aagctgaatg gccaggccga gctgttctac 4021 cgccctaagt ccaggatgaa gaggatggca caccggctgg gagagaagat gctgaacaag 4081 aagctgaagg atcagaaaac cccaatcccc gacaccctgt accaggagct gtacgactat 4141 gtgaatcaca gactgtccca cgacctgtct gatgaggcca gggccctgct gcccaacgtg 4201 atcaccaagg aggtgtctca cgagatcatc aaggataggc gctttaccag cgacaagttc 4261 tttttccacg tgcctatcac actgaactat caggccgcca attccccatc taagttcaac 4321 cagagggtga atgcctacct gaaggagcac cccgagacac ctatcatcgg catcgcccgg 4381 ggtgagagaa acctgatcta tatcacagtg atcgactcca ccggcaagat cctggagcag 4441 cggagcctga acaccatcca gcagtttgat taccagaaga agctggacaa cagggagaag 4501 gagagggtgg cagcaaggca ggcctggtct gtggtgggca caatcaagga tctgaagcag 4561 ggctatctga gccaggtcat ccacgagatc gtggacctga tgatccacta ccaggccgtg 4621 gtggtgctgg agaacctgaa tttcggcttt aagagcaaga ggaccggcat cgccgagaag 4681 gccgtgtacc agcagttcga gaagatgctg atcgataagc tgaattgcct ggtgctgaag 4741 gactatccag cagagaaagt gggaggcgtg ctgaacccat accagctgac agaccagttc 4801 acctcctttg ccaagatggg cacccagtct ggcttcctgt tttacgtgcc tgccccatat 4861 acatctaaga tcgatcccct gaccggcttc gtggacccct tcgtgtggaa aaccatcaag 4921 aatcacgaga gccgcaagca cttcctggag ggcttcgact ttctgcacta cgacgtgaaa 4981 accggcgact tcatcctgca ctttaagatg aacagaaatc tgtccttcca gaggggcctg 5041 cccggcttta tgcctgcatg ggatatcgtg ttcgagaaga acgagacaca gtttgacgcc 5101 aagggcaccc ctttcatcgc cggcaagaga atcgtgccag tgatcgagaa tcacagattc 5161 accggcagat accgggacct gtatcctgcc aacgagctga tcgccctgct ggaggagaag 5221 ggcatcgtgt tcagggatgg ctccaacatc ctgccaaagc tgctggagaa tgacgattct 5281 cacgccatcg acaccatggt ggccctgatc cgcagcgtgc tgcagatgcg gaactccaat 5341 gccgccacag gcgaggacta tatcaacagc cccgtgcgcg atctgaatgg cgtgtgcttc 5401 gactcccggt ttcagaaccc agagtggccc atggacgccg atgccaatgg cgcctaccac 5461 atcgccctga agggccagct gctgctgaat cacctgaagg agagcaagga tctgaagctg 5521 cagaacggca tctccaatca ggactggctg gcctacatcc aggagctgcg caactctggc 5581 ggctcaaaaa gaaccgccga cggcagcgaa ttcgagccca agaagaagag gaaagtctaa 5641 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt 5701 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg 5761 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt 5821 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca 5881 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct 5941 ggggctcgat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc 6001 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 6061 gtaaagccta gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 6121 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 6181 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 6241 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 6301 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 6361 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 6421 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 6481 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 6541 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 6601 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 6661 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 6721 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 6781 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 6841 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 6901 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 6961 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 7021 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 7081 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 7141 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 7201 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 7261 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 7321 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 7381 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 7441 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 7501 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 7561 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 7621 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 7681 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 7741 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 7801 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 7861 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 7921 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 7981 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 8041 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 8101 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc 8161 gatctcccga tcccctaggg tcgactctca gtacaatctg ctctgatgcc gcatagttaa 8221 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt 8281 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc 8341 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta 8401 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg 8461 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga 8521 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat 8581 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat c //