LOCUS       Exported                8631 bp ds-DNA     circular UNA 31-DEC-2019
DEFINITION  ABEmax10.
ACCESSION   urn.local...c-8wnwdza
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 2, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8631
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     source          1654..5544
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          5545..5574
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          5575..5586
     primer_bind     234..253
                     /standard_name="LCv2_seq1"
                     /label=LCv2_seq1
     primer_bind     261..285
                     /standard_name="JLseq007_ACK15"
                     /label=JLseq007_ACK15
     primer_bind     261..285
                     /standard_name="ACK15"
                     /label=ACK15
     promoter        275..356
                     /note="Geneious type: Promoter"
     primer_bind     286..306
                     /standard_name="QB0012 CMV Forward"
                     /label=QB0012 CMV Forward
     primer_bind     286..306
                     /standard_name="Eton CMV-Forward"
                     /label=Eton CMV-Forward
     primer_bind     286..306
                     /standard_name="Genewiz CMV-Forward"
                     /label=Genewiz CMV-Forward
     primer_bind     293..314
                     /standard_name="QB0976 pRLuc-MCS-F"
                     /label=QB0976 pRLuc-MCS-F
     primer_bind     complement(331..360)
                     /standard_name="352_CMV_Rev2"
                     /label=352_CMV_Rev2
     primer_bind     377..396
                     /standard_name="T7 promoter"
                     /label=T7 promoter
     primer_bind     377..396
                     /standard_name="QB0106 T7"
                     /label=QB0106 T7
     primer_bind     377..396
                     /standard_name="Eton T7 Promoter"
                     /label=Eton T7 Promoter
     primer_bind     377..396
                     /standard_name="Genewiz T7"
                     /label=Genewiz T7
     primer_bind     377..396
                     /standard_name="T7"
                     /label=T7
     promoter        377..395
                     /note="Geneious type: Promoter"
     primer_bind     complement(382..408)
                     /standard_name="197_pCMV_bb_F"
                     /label=197_pCMV_bb_F
     primer_bind     complement(385..408)
                     /standard_name="203_bb_NpuC_R"
                     /label=203_bb_NpuC_R
     primer_bind     complement(389..447)
                     /standard_name="875_Genscript_APOBEC-bpnls-rev"
                     /label=875_Genscript_APOBEC-bpnls-rev
     primer_bind     389..428
                     /standard_name="903_GS_NpuN-fwd"
                     /label=903_GS_NpuN-fwd
     primer_bind     complement(389..408)
                     /standard_name="122_CMV_rev"
                     /label=122_CMV_rev
     primer_bind     complement(389..408)
                     /standard_name="CMV_rev"
                     /label=CMV_rev
     misc_feature    406..408
                     /standard_name="USER1"
                     /label=USER1
     CDS             409..465
                     /standard_name="BPNLS"
                     /label=BPNLS
     primer_bind     428..487
                     /standard_name="925_Genscript_ABE-BPNLS-fwd"
                     /label=925_Genscript_ABE-BPNLS-fwd
     CDS             466..1059
                     /standard_name="ABE 6.20"
                     /label=ABE 6.20
     primer_bind     690..714
                     /standard_name="KZ918"
                     /label=KZ918
     misc_feature    complement(690..714)
                     /standard_name="KZ922"
                     /label=KZ922
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1042..1062)
                     /standard_name="KZ1424"
                     /label=KZ1424
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     1053..1083
                     /standard_name="KZ1423"
                     /label=KZ1423
     CDS             1060..1557
                     /standard_name="max10"
                     /label=max10
     misc_feature    complement(1539..1558)
                     /standard_name="KZ1428"
                     /label=KZ1428
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     1552..1577
                     /standard_name="KZ1427"
                     /label=KZ1427
     misc_feature    1558..1653
                     /standard_name="3xGGS-XTEN-3xGGS"
                     /label=3xGGS-XTEN-3xGGS
                     /note="Geneious type: Misc"
     primer_bind     complement(1636..1655)
                     /standard_name="EE 134"
                     /label=EE 134
     primer_bind     1642..1677
                     /standard_name="EE 133"
                     /label=EE 133
     misc_feature    1642..1655
                     /standard_name="USER"
                     /label=USER
                     /note="Geneious type: overhang"
     CDS             1654..5544
                     /codon_start=1
                     /standard_name="AsCpf1"
                     /product="CRISPR-associated protein from Acidaminococcus
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
                     HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
                     AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
                     KFTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
                     ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
                     NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
                     RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
                     KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
                     KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
                     RNYATKKPYSVEKFKLNFQMPTLARGWDVNREKNNGAILFVKNGLYYLGIMPKQKGRYK
                     ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
                     LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
                     LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
                     HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
                     DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
                     HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIARGERNLIYITVIDSTGKILEQR
                     SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
                     VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
                     FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
                     VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
                     HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
                     RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
                     KDLKLQNGISNQD"
     misc_feature    1654..5543
                     /standard_name="GACAAGAAGTACAGCATCGGCCTGGCCATCGGCACCAACTCT 
                     GTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGCAAGAAATTCAAGGTGC 
                     TGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGAGCCCTGCTGTTCGA 
                     CAGCGGCGAAACAGCCGAGGCCACCCGGCTGAAGAGAACCGCCAGAAGAAGATACACC 
                     AGACGGAAGAACCGGATCTGCTATCTGCAAGAGATCTTCAGCAACGAGATGGCCAAGG 
                     TGGACGACAGCTTCTTCCACAGACTGGAAGAGTCCTTCCTGGTGGAAGAGGATAAGAA 
                     GCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAAG 
                     TACCCCACCATCTACCACCTGAGAAAGAAACTGGTGGACAGCACCGACAAGGCCGACC 
                     TGCGGCTGATCTATCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGAT 
                     CGAGGGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTG 
                     CAGACCTACAACCAGCTGTTCGAGGAAAACCCCATCAACGCCAGCGGCGTGGACGCCA 
                     AGGCCATCCTGTCTGCCAGACTGAGCAAGAGCAGACGGCTGGAAAATCTGATCGCCCA 
                     GCTGCCCGGCGAGAAGAAGAATGGCCTGTTCGGAAACCTGATTGCCCTGAGCCTGGGC 
                     CTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGATGCCAAACTGCAGCTGA 
                     GCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTA 
                     CGCCGACCTGTTTCTGGCCGCCAAGAACCTGTCCGACGCCATCCTGCTGAGCGACATC 
                     CTGAGAGTGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCTCTATGATCAAGAGAT 
                     ACGACGAGCACCACCAGGACCTGACCCTGCTGAAAGCTCTCGTGCGGCAGCAGCTGCC 
                     TGAGAAGTACAAAGAGATTTTCTTCGACCAGAGCAAGAACGGCTACGCCGGCTACATT 
                     GACGGCGGAGCCAGCCAGGAAGAGTTCTACAAGTTCATCAAGCCCATCCTGGAAAAGA 
                     TGGACGGCACCGAGGAACTGCTCGTGAAGCTGAACAGAGAGGACCTGCTGCGGAAGCA 
                     GCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGGGAGAGCTGCACGCC 
                     ATTCTGCGGCGGCAGGAAGATTTTTACCCATTCCTGAAGGACAACCGGGAAAAGATCG 
                     AGAAGATCCTGACCTTCCGCATCCCCTACTACGTGGGCCCTCTGGCCAGGGGAAACAG 
                     CAGATTCGCCTGGATGACCAGAAAGAGCGAGGAAACCATCACCCCCTGGAACTTCGAG 
                     GAAGTGGTGGACAAGGGCGCTTCCGCCCAGAGCTTCATCGAGCGGATGACCAACTTCG 
                     ATAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTT 
                     CACCGTGTATAACGAGCTGACCAAAGTGAAATACGTGACCGAGGGAATGAGAAAGCCC 
                     GCCTTCCTGAGCGGCGAGCAGAAAAAGGCCATCGTGGACCTGCTGTTCAAGACCAACC 
                     GGAAAGTGACCGTGAAGCAGCTGAAAGAGGACTACTTCAAGAAAATCGAGTGCTTCGA 
                     CTCCGTGGAAATCTCCGGCGTGGAAGATCGGTTCAACGCCTCCCTGGGCACATACCAC 
                     GATCTGCTGAAAATTATCAAGGACAAGGACTTCCTGGACAATGAGGAAAACGAGGACA 
                     TTCTGGAAGATATCGTGCTGACCCTGACACTGTTTGAGGACAGAGAGATGATCGAGGA 
                     ACGGCTGAAAACCTATGCCCACCTGTTCGACGACAAAGTGATGAAGCAGCTGAAGCGG 
                     CGGAGATACACCGGCTGGGGCAGGCTGAGCCGGAAGCTGATCAACGGCATCCGGGACA 
                     AGCAGTCCGGCAAGACAATCCTGGATTTCCTGAAGTCCGACGGCTTCGCCAACAGAAA 
                     CTTCATGCAGCTGATCCACGACGACAGCCTGACCTTTAAAGAGGACATCCAGAAAGCC 
                     CAGGTGTCCGGCCAGGGCGATAGCCTGCACGAGCACATTGCCAATCTGGCCGGCAGCC 
                     CCGCCATTAAGAAGGGCATCCTGCAGACAGTGAAGGTGGTGGACGAGCTCGTGAAAGT 
                     GATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAAATGGCCAGAGAGAACCAGACC 
                     ACCCAGAAGGGACAGAAGAACAGCCGCGAGAGAATGAAGCGGATCGAAGAGGGCATCA 
                     AAGAGCTGGGCAGCCAGATCCTGAAAGAACACCCCGTGGAAAACACCCAGCTGCAGAA 
                     CGAGAAGCTGTACCTGTACTACCTGCAGAATGGGCGGGATATGTACGTGGACCAGGAA 
                     CTGGACATCAACCGGCTGTCCGACTACGATGTGGACCATATCGTGCCTCAGAGCTTTC 
                     TGAAGGACGACTCCATCGACAACAAGGTGCTGACCAGAAGCGACAAGAACCGGGGCAA 
                     GAGCGACAACGTGCCCTCCGAAGAGGTCGTGAAGAAGATGAAGAACTACTGGCGGCAG 
                     CTGCTGAACGCCAAGCTGATTACCCAGAGAAAGTTCGACAATCTGACCAAGGCCGAGA 
                     GAGGCGGCCTGAGCGAACTGGATAAGGCCGGCTTCATCAAGAGACAGCTGGTGGAAAC 
                     CCGGCAGATCACAAAGCACGTGGCACAGATCCTGGACTCCCGGATGAACACTAAGTAC 
                     GACGAGAATGACAAGCTGATCCGGGAAGTGAAAGTGATCACCCTGAAGTCCAAGCTGG 
                     TGTCCGATTTCCGGAAGGATTTCCAGTTTTACAAAGTGCGCGAGATCAACAACTACCA 
                     CCACGCCCACGACGCCTACCTGAACGCCGTCGTGGGAACCGCCCTGATCAAAAAGTAC 
                     CCTAAGCTGGAAAGCGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGA 
                     TGATCGCCAAGAGCGAGCAGGAAATCGGCAAGGCTACCGCCAAGTACTTCTTCTACAG 
                     CAACATCATGAACTTTTTCAAGACCGAGATTACCCTGGCCAACGGCGAGATCCGGAAG 
                     CGGCCTCTGATCGAGACAAACGGCGAAACCGGGGAGATCGTGTGGGATAAGGGCCGGG 
                     ATTTTGCCACCGTGCGGAAAGTGCTGAGCATGCCCCAAGTGAATATCGTGAAAAAGAC 
                     CGAGGTGCAGACAGGCGGCTTCAGCAAAGAGTCTATCCTGCCCAAGAGGAACAGCGAT 
                     AAGCTGATCGCCAGAAAGAAGGACTGGGACCCTAAGAAGTACGGCGGCTTCGACAGCC 
                     CCACCGTGGCCTATTCTGTGCTGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCAAGAA 
                     ACTGAAGAGTGTGAAAGAGCTGCTGGGGATCACCATCATGGAAAGAAGCAGCTTCGAG 
                     AAGAATCCCATCGACTTTCTGGAAGCCAAGGGCTACAAAGAAGTGAAAAAGGACCTGA 
                     TCATCAAGCTGCCTAAGTACTCCCTGTTCGAGCTGGAAAACGGCCGGAAGAGAATGCT 
                     GGCCTCTGCCGGCGAACTGCAGAAGGGAAACGAACTGGCCCTGCCCTCCAAATATGTG 
                     AACTTCCTGTACCTGGCCAGCCACTATGAGAAGCTGAAGGGCTCCCCCGAGGATAATG 
                     AGCAGAAACAGCTGTTTGTGGAACAGCACAAGCACTACCTGGACGAGATCATCGAGCA 
                     GATCAGCGAGTTCTCCAAGAGAGTGATCCTGGCCGACGCTAATCTGGACAAAGTGCTG 
                     TCCGCCTACAACAAGCACCGGGATAAGCCCATCAGAGAGCAGGCCGAGAATATCATCC 
                     ACCTGTTTACCCTGACCAATCTGGGAGCCCCTGCCGCCTTCAAGTACTTTGACACCAC 
                     CATCGACCGGAAGAGGTACACCAGCACCAAAGAGGTGCTGGACGCCACCCTGATCCAC 
                     CAGAGCATCACCGGCCTGTACGAGACACGGATCGACCTGTCTCAGCTGGGAGGTGACT 
                     CTGGCGGCTCAAAAAGAACCGC"
                     /label=Geneious type: Editing History Replacement
                     /note="Geneious type: Editing History Replacement"
     primer_bind     complement(4365..4383)
                     /standard_name="EE 218"
                     /label=EE 218
     primer_bind     4372..4395
                     /standard_name="EE 217"
                     /label=EE 217
     misc_feature    4372..4383
                     /standard_name="USER"
                     /label=USER
                     /note="Geneious type: overhang"
     regulatory      5294..5303
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
     misc_feature    5545..5598
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
     CDS             5545..5574
                     /codon_start=1
                     /standard_name="AsCpf1"
                     /product="CRISPR-associated protein from Acidaminococcus
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="MLAYIQELRN"
     primer_bind     complement(5552..5577)
                     /standard_name="EE 136"
                     /label=EE 136
     primer_bind     5564..5597
                     /standard_name="EE 135"
                     /label=EE 135
     misc_feature    5564..5577
                     /standard_name="USER"
                     /label=USER
                     /note="Geneious type: overhang"
     CDS             5587..5598
                     /standard_name="BPNLS"
                     /label=BPNLS
     CDS             5599..5637
                     /standard_name="BPNLS"
                     /label=BPNLS
     primer_bind     5600..5622
                     /standard_name="KZ1406"
                     /label=KZ1406
     misc_feature    5600..5612
                     /standard_name="USER"
                     /label=USER
                     /note="Geneious type: overhang"
     terminator      5689..5916
                     /note="Geneious type: Terminator"
     primer_bind     5715..5733
                     /standard_name="436_bGH_QPCRF2"
                     /label=436_bGH_QPCRF2
     primer_bind     complement(5838..5862)
                     /standard_name="ACK64 (end)"
                     /label=ACK64 (end)
     misc_feature    complement(5838..5862)
                     /standard_name="ACK64"
                     /label=ACK64
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(5852..5871)
                     /standard_name="438_bGH_QPCRR2"
                     /label=438_bGH_QPCRR2
     primer_bind     complement(5857..5875)
                     /standard_name="439_bGH_QPCRR3"
                     /label=439_bGH_QPCRR3
     primer_bind     complement(5877..5896)
                     /standard_name="437_bGH_QPCRR1"
                     /label=437_bGH_QPCRR1
     primer_bind     complement(5897..5916)
                     /standard_name="920_bGH-BamHI-rev"
                     /label=920_bGH-BamHI-rev
     primer_bind     5975..6001
                     /standard_name="654_CAG_fwd"
                     /label=654_CAG_fwd
     primer_bind     complement(5987..6003)
                     /standard_name="Genewiz M13R"
                     /label=Genewiz M13R
     primer_bind     complement(5987..6003)
                     /standard_name="QB0048 M13 Reverse"
                     /label=QB0048 M13 Reverse
     primer_bind     complement(5987..6003)
                     /standard_name="Eton M13R Reverse"
                     /label=Eton M13R Reverse
     primer_bind     complement(5987..6003)
                     /standard_name="M13-R (-26)"
                     /label=M13-R (-26)
     primer_bind     complement(6000..6023)
                     /standard_name="QB0108 Tac promoter"
                     /label=QB0108 Tac promoter
     primer_bind     complement(6000..6023)
                     /standard_name="M13-R (-46)"
                     /label=M13-R (-46)
     primer_bind     complement(6000..6022)
                     /standard_name="QB0050 M13/pUC Reverse"
                     /label=QB0050 M13/pUC Reverse
     promoter        complement(6036..6065)
                     /note="Geneious type: Promoter"
     primer_bind     complement(6072..6091)
                     /standard_name="836_Lac-F"
                     /label=836_Lac-F
     primer_bind     complement(6072..6091)
                     /standard_name="Lac-F (EtonSeq)"
                     /label=Lac-F (EtonSeq)
     primer_bind     6080..6104
                     /standard_name="ACK21"
                     /label=ACK21
     misc_feature    complement(6080..6104)
                     /standard_name="ACK32"
                     /label=ACK32
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(6218..6235)
                     /standard_name="QB0039 L4440"
                     /label=QB0039 L4440
     primer_bind     complement(6270..6289)
                     /standard_name="VR"
                     /label=VR
     primer_bind     complement(6359..6380)
                     /standard_name="11_pFYF_Seq_Rev"
                     /label=11_pFYF_Seq_Rev
     misc_feature    complement(6374..6993)
                     /standard_name="pBR322 origin"
                     /label=pBR322 origin
                     /note="Geneious type: Origin of Replication"
     primer_bind     6389..6413
                     /standard_name="ACK10"
                     /label=ACK10
     primer_bind     complement(6450..6469)
                     /standard_name="BR322-F"
                     /label=BR322-F
     misc_feature    6585
                     /label=Geneious type: Editing History Insertion
                     /note="Geneious type: Editing History Insertion"
     primer_bind     6612..6630
                     /standard_name="QB0766 RepO-F"
                     /label=QB0766 RepO-F
     primer_bind     6709..6728
                     /standard_name="833_BR322-rev1"
                     /label=833_BR322-rev1
     primer_bind     complement(6737..6756)
                     /standard_name="QB0767 RepO-R"
                     /label=QB0767 RepO-R
     misc_feature    6830
                     /standard_name="G"
                     /label=G
                     /note="Geneious type: Editing History Replacement"
     primer_bind     6890..6914
                     /standard_name="ACK11"
                     /label=ACK11
     primer_bind     complement(6890..6909)
                     /standard_name="832_BR322-fwd1"
                     /label=832_BR322-fwd1
     misc_feature    complement(7148..8008)
                     /standard_name="amp marker"
                     /label=amp marker
                     /note="Geneious type: Marker"
     primer_bind     7389..7413
                     /standard_name="ACK12"
                     /label=ACK12
     primer_bind     7554..7573
                     /standard_name="ACK563"
                     /label=ACK563
     primer_bind     7771..7790
                     /standard_name="QB0005 Amp-R"
                     /label=QB0005 Amp-R
     primer_bind     complement(7856..7883)
                     /standard_name="562_Amp_Fwd"
                     /label=562_Amp_Fwd
     primer_bind     7856..7883
                     /standard_name="563_Amp_Rev"
                     /label=563_Amp_Rev
     primer_bind     7889..7913
                     /standard_name="ACK13"
                     /label=ACK13
     promoter        complement(8050..8078)
                     /note="Geneious type: Promoter"
     primer_bind     8122..8139
                     /standard_name="QB0090 pQE promoter"
                     /label=QB0090 pQE promoter
     primer_bind     complement(8192..8211)
                     /standard_name="QB0092 pRS-marker"
                     /label=QB0092 pRS-marker
     primer_bind     8392..8416
                     /standard_name="JLseq006_ACK14"
                     /label=JLseq006_ACK14
     primer_bind     8392..8416
                     /standard_name="ACK14"
                     /label=ACK14
     promoter        join(8399..8631,1..356)
     primer_bind     8399..8442
                     /standard_name="387_pAAV_fwd"
                     /label=387_pAAV_fwd
     primer_bind     complement(8449..8468)
                     /standard_name="QB1110 pSicoR"
                     /label=QB1110 pSicoR
     misc_feature    8588
                     /standard_name="C"
                     /label=C
                     /note="Geneious type: Editing History Replacement"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
      421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctctga agtcgagttt
      481 agccacgagt attggatgag gcacgcactg accctggcaa agcgagcatg ggatgaaaga
      541 gaagtccccg tgggcgccgt gctggtgcac aacaatagag tgatcggaga gggatggaac
      601 aggccaatcg gccgccacga ccctaccgca cacgcagaga tcatggcact gaggcaggga
      661 ggcctggtca tgcagaatta ccgcctgatc gatgccaccc tgtatgtgac actggagcca
      721 tgcgtgatgt gcgcaggagc aatgatccac agcaggatcg gaagagtggt gttcggagca
      781 cgggacgcca agaccggcgc agcaggctcc ctgatggatg tgctgcacca ccccggcatg
      841 aaccaccggg tggagatcac agagggaatc ctggcagacg agtgcgccgc cctgctgagc
      901 gatttcttta gaatgcggag acaggagatc aaggcccaga agaaggcaca gagctccacc
      961 gactctggag gatctagcgg aggatcctct ggaagcgaga caccaggcac aagcgagtcc
     1021 gccacaccag agagctccgg cggctcctcc ggaggatcct ctgaggtgga gttttcccac
     1081 gagtactgga tgagacatgc cctgaccctg gccaagaggg cacgggatga gagggaggtg
     1141 cctgtgggag ccgtgctggt gctgaacaat agagtgatcg gcgagggctg gaacagagcc
     1201 atcggcctgc acgacccaac agcccatgcc gaaattatgg ccctgagaca gggcggcctg
     1261 gtcatgcaga actacagact gattgacgcc accctgtacg tgacattcga gccttgcgtg
     1321 atgtgcgccg gcgccatgat ccactctagg atcggccgcg tggtgtttgg cgtgaggaac
     1381 tcaaaaagag gcgccgcagg ctccctgatg aacgtgctga actaccccgg catgaatcac
     1441 cgcgtcgaaa ttaccgaggg aatcctggca gatgaatgtg ccgccctgct gtgcgatttc
     1501 tatcggatgc ctagacaggt gttcaatgct cagaagaagg cccagagctc catcaactcc
     1561 ggaggatcta gcggaggctc ctctggctct gagacacctg gcacaagcga gagcgcaaca
     1621 cctgaaagca gcgggggcag cagcgggggg tcaatgacac agttcgaggg ctttaccaac
     1681 ctgtatcagg tgagcaagac actgcggttt gagctgatcc cacagggcaa gaccctgaag
     1741 cacatccagg agcagggctt catcgaggag gacaaggccc gcaatgatca ctacaaggag
     1801 ctgaagccca tcatcgatcg gatctacaag acctatgccg accagtgcct gcagctggtg
     1861 cagctggatt gggagaacct gagcgccgcc atcgactcct atagaaagga gaaaaccgag
     1921 gagacaagga acgccctgat cgaggagcag gccacatatc gcaatgccat ccacgactac
     1981 ttcatcggcc ggacagacaa cctgaccgat gccatcaata agagacacgc cgagatctac
     2041 aagggcctgt tcaaggccga gctgtttaat ggcaaggtgc tgaagcagct gggcaccgtg
     2101 accacaaccg agcacgagaa cgccctgctg cggagcttcg acaagtttac aacctacttc
     2161 tccggctttt atagaaacag gaagaacgtg ttcagcgccg aggatatcag cacagccatc
     2221 ccacaccgca tcgtgcagga caacttcccc aagtttaagg agaattgtca catcttcaca
     2281 cgcctgatca ccgccgtgcc cagcctgcgg gagcactttg agaacgtgaa gaaggccatc
     2341 ggcatcttcg tgagcacctc catcgaggag gtgttttcct tcccttttta taaccagctg
     2401 ctgacacaga cccagatcga cctgtataac cagctgctgg gaggaatctc tcgggaggca
     2461 ggcaccgaga agatcaaggg cctgaacgag gtgctgaatc tggccatcca gaagaatgat
     2521 gagacagccc acatcatcgc ctccctgcca cacagattca tccccctgtt taagcagatc
     2581 ctgtccgata ggaacaccct gtctttcatc ctggaggagt ttaagagcga cgaggaagtg
     2641 atccagtcct tctgcaagta caagacactg ctgagaaacg agaacgtgct ggagacagcc
     2701 gaggccctgt ttaacgagct gaacagcatc gacctgacac acatcttcat cagccacaag
     2761 aagctggaga caatcagcag cgccctgtgc gaccactggg atacactgag gaatgccctg
     2821 tatgagcgga gaatctccga gctgacaggc aagatcacca agtctgccaa ggagaaggtg
     2881 cagcgcagcc tgaagcacga ggatatcaac ctgcaggaga tcatctctgc cgcaggcaag
     2941 gagctgagcg aggccttcaa gcagaaaacc agcgagatcc tgtcccacgc acacgccgcc
     3001 ctggatcagc cactgcctac aaccctgaag aagcaggagg agaaggagat cctgaagtct
     3061 cagctggaca gcctgctggg cctgtaccac ctgctggact ggtttgccgt ggatgagtcc
     3121 aacgaggtgg accccgagtt ctctgcccgg ctgaccggca tcaagctgga gatggagcct
     3181 tctctgagct tctacaacaa ggccagaaat tatgccacca agaagcccta ctccgtggag
     3241 aagttcaagc tgaactttca gatgcctaca ctggccagag gctgggacgt gaatagagag
     3301 aagaacaatg gcgccatcct gtttgtgaag aacggcctgt actatctggg catcatgcca
     3361 aagcagaagg gcaggtataa ggccctgagc ttcgagccca cagagaaaac cagcgagggc
     3421 tttgataaga tgtactatga ctacttccct gatgccgcca agatgatccc aaagtgcagc
     3481 acccagctga aggccgtgac agcccacttt cagacccaca caacccccat cctgctgtcc
     3541 aacaatttca tcgagcctct ggagatcaca aaggagatct acgacctgaa caatcctgag
     3601 aaggagccaa agaagtttca gacagcctac gccaagaaaa ccggcgacca gaagggctac
     3661 agagaggccc tgtgcaagtg gatcgacttc acaagggatt ttctgtccaa gtataccaag
     3721 acaacctcta tcgatctgtc tagcctgcgg ccatcctctc agtataagga cctgggcgag
     3781 tactatgccg agctgaatcc cctgctgtac cacatcagct tccagagaat cgccgagaag
     3841 gagatcatgg atgccgtgga gacaggcaag ctgtacctgt tccagatcta taacaaggac
     3901 tttgccaagg gccaccacgg caagcctaat ctgcacacac tgtattggac cggcctgttt
     3961 tctccagaga acctggccaa gacaagcatc aagctgaatg gccaggccga gctgttctac
     4021 cgccctaagt ccaggatgaa gaggatggca caccggctgg gagagaagat gctgaacaag
     4081 aagctgaagg atcagaaaac cccaatcccc gacaccctgt accaggagct gtacgactat
     4141 gtgaatcaca gactgtccca cgacctgtct gatgaggcca gggccctgct gcccaacgtg
     4201 atcaccaagg aggtgtctca cgagatcatc aaggataggc gctttaccag cgacaagttc
     4261 tttttccacg tgcctatcac actgaactat caggccgcca attccccatc taagttcaac
     4321 cagagggtga atgcctacct gaaggagcac cccgagacac ctatcatcgg catcgcccgg
     4381 ggtgagagaa acctgatcta tatcacagtg atcgactcca ccggcaagat cctggagcag
     4441 cggagcctga acaccatcca gcagtttgat taccagaaga agctggacaa cagggagaag
     4501 gagagggtgg cagcaaggca ggcctggtct gtggtgggca caatcaagga tctgaagcag
     4561 ggctatctga gccaggtcat ccacgagatc gtggacctga tgatccacta ccaggccgtg
     4621 gtggtgctgg agaacctgaa tttcggcttt aagagcaaga ggaccggcat cgccgagaag
     4681 gccgtgtacc agcagttcga gaagatgctg atcgataagc tgaattgcct ggtgctgaag
     4741 gactatccag cagagaaagt gggaggcgtg ctgaacccat accagctgac agaccagttc
     4801 acctcctttg ccaagatggg cacccagtct ggcttcctgt tttacgtgcc tgccccatat
     4861 acatctaaga tcgatcccct gaccggcttc gtggacccct tcgtgtggaa aaccatcaag
     4921 aatcacgaga gccgcaagca cttcctggag ggcttcgact ttctgcacta cgacgtgaaa
     4981 accggcgact tcatcctgca ctttaagatg aacagaaatc tgtccttcca gaggggcctg
     5041 cccggcttta tgcctgcatg ggatatcgtg ttcgagaaga acgagacaca gtttgacgcc
     5101 aagggcaccc ctttcatcgc cggcaagaga atcgtgccag tgatcgagaa tcacagattc
     5161 accggcagat accgggacct gtatcctgcc aacgagctga tcgccctgct ggaggagaag
     5221 ggcatcgtgt tcagggatgg ctccaacatc ctgccaaagc tgctggagaa tgacgattct
     5281 cacgccatcg acaccatggt ggccctgatc cgcagcgtgc tgcagatgcg gaactccaat
     5341 gccgccacag gcgaggacta tatcaacagc cccgtgcgcg atctgaatgg cgtgtgcttc
     5401 gactcccggt ttcagaaccc agagtggccc atggacgccg atgccaatgg cgcctaccac
     5461 atcgccctga agggccagct gctgctgaat cacctgaagg agagcaagga tctgaagctg
     5521 cagaacggca tctccaatca ggactggctg gcctacatcc aggagctgcg caactctggc
     5581 ggctcaaaaa gaaccgccga cggcagcgaa ttcgagccca agaagaagag gaaagtctaa
     5641 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
     5701 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     5761 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     5821 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     5881 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     5941 ggggctcgat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     6001 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6061 gtaaagccta gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6121 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6181 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6241 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     6301 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     6361 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     6421 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     6481 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     6541 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     6601 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     6661 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     6721 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     6781 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     6841 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     6901 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     6961 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7021 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7081 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7141 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7201 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7261 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     7321 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     7381 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     7441 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     7501 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     7561 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     7621 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     7681 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     7741 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     7801 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     7861 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     7921 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     7981 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8041 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8101 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     8161 gatctcccga tcccctaggg tcgactctca gtacaatctg ctctgatgcc gcatagttaa
     8221 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
     8281 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
     8341 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
     8401 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     8461 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     8521 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     8581 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat c
//
