LOCUS       Exported                5189 bp ds-DNA     circular SYN 31-DEC-2019
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...8l-b7fdfaq
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5189)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 2, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5189
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      complement(11..52)
     modified_base   53..65
     CDS             complement(66..845)
                     /standard_name="aadA"
                     /label=aadA
     promoter        858..942
     misc_feature    914
                     /standard_name="G>T vs original map"
                     /label=G>T vs original map
     rep_origin      958..1546
     misc_feature    958
                     /standard_name="T deletion"
                     /label=T deletion
     CDS             complement(1974..2165)
                     /standard_name="Rop"
                     /label=Rop
     modified_base   2261..2275
     terminator      2276..2320
     misc_feature    complement(2315..2342)
                     /standard_name="primer"
                     /label=primer
     misc_feature    2334..2342
                     /standard_name="USER junction"
                     /label=USER junction
     promoter        2405..2410
     misc_feature    2411..2429
                     /standard_name="oligo overlap"
                     /label=oligo overlap
     promoter        2428..2433
     misc_feature    2430..2432
                     /standard_name="diff"
                     /label=diff
     prim_transcript 2440
                     /standard_name="+1 TSS"
                     /label=+1 TSS
     misc_feature    2442..2466
                     /standard_name="homology"
                     /label=homology
     misc_feature    2519..2533
                     /standard_name="syn RBS 0.4k"
                     /label=syn RBS 0.4k
     misc_feature    2534..5185
                     /standard_name="T7 RNAP"
                     /label=T7 RNAP
     misc_feature    complement(2686..2688)
                     /standard_name="PAM"
                     /label=PAM
     misc_feature    complement(2689..2708)
                     /standard_name="gRNA"
                     /label=gRNA
     misc_feature    complement(2701..2703)
                     /standard_name="CAC excellent site"
                     /label=CAC excellent site
     misc_feature    2702..2707
                     /standard_name="RQ"
                     /label=RQ
     misc_feature    complement(2702..2705)
                     /standard_name="window 4-7"
                     /label=window 4-7
     misc_feature    complement(2704..2706)
                     /standard_name="TAT excellent site"
                     /label=TAT excellent site
     misc_feature    2707..2716
                     /standard_name="USER jct 20"
                     /label=USER jct 20
     misc_feature    5166..5184
                     /standard_name="prime"
                     /label=prime
     misc_feature    5183..5186
                     /standard_name="TAAT*"
                     /label=TAAT*
ORIGIN
        1 actcctcagc aaatataatg accctcttga taacccaaga gggcattttt taatgcccat
       61 ggcgtttatt tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc
      121 aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct
      181 tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca
      241 tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact
      301 acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt
      361 agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct
      421 accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc
      481 gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt
      541 tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct
      601 acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc
      661 aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca
      721 ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc
      781 ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg
      841 atcaccgctt ccctcatact cttccttttt caatattatt gaagcattta tcagggttat
      901 tgtctcatga gcgtatacat atttgaatgt atttagaaaa ataggccaaa taggccgttg
      961 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1021 ggtggtttgt ttgccggatc aagagctacc acctcttttt ccgaaggtaa ctggcttcag
     1081 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     1141 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1201 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1261 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1321 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     1381 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     1441 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     1501 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     1561 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     1621 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     1681 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggatgagc gcctgatgcg
     1741 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     1801 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     1861 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     1921 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     1981 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg
     2041 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga
     2101 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg
     2161 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa
     2221 cgagagagga tgctcacgat acgggttact gatgatgaac aagaggacat ccggtcaaat
     2281 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt gctgaggaga cttagggact
     2341 ctcacagcta acaccacgtc gtccctatct gctgccctag gtctatgagt ggttgctgga
     2401 taactttacg ggcatgcata aggctcgtat gatatattca gggagaccac aacggtttcc
     2461 ctctacaaat aattttgttt aacttttact agagtgggac cctacctgca ggtgcagtac
     2521 tacatcatca ggcatgaaca cgattaacat cgctaagaac gacttctctg acatcgaact
     2581 ggctgctatc ccgttcaaca ctctggctga ccattacggt gagcgtttag ctcgcgaaca
     2641 gttggccctt gagcatgagt cttacgagat gggtgaagca cgcttccgca agatgtttga
     2701 gtgataactt aaagctggtg aggttgcgga taacgctgcc gccaagcctc tcatcactac
     2761 cctactccct aagatgattg cacgcatcaa cgactggttt gaggaagtga aagctaagcg
     2821 cggcaagcgc ccgacagcct tccagttcct gcaagaaatc aagccggaag ccgtagcgta
     2881 catcaccatt aagaccactc tggcttgcct aaccagtgct gacaatacaa ccgttcaggc
     2941 tgtagcaagc gcaatcggtc gggccattga ggacgaggct cgcttcggtc gtatccgtga
     3001 ccttaaagct aagcacttca agaaaaacgt tgaggaacaa ctcaacaagc gcgtagggca
     3061 cgtctacaag aaagcattta tgcaagttgt cgaggctgac atgctctcta agggtctact
     3121 cggtggcgag gcgtggtctt cgtggcataa ggaagactct attcatgtag gagtacgctg
     3181 catcgagatg ctcattgagt caaccggaat ggttagctta caccgccaaa atgctggcgt
     3241 agtaggtcaa gactctgaga ctatcgaact cgcacctgaa tacgctgagg ctatcgcaac
     3301 ccgtgcaggt gcgctggctg gcatctctcc gatgttccaa ccttgcgtag ttcctcctaa
     3361 gccgtggact ggcattactg gtggtggcta ttgggctaac ggtcgtcgtc ctctggcgct
     3421 ggtgcgtact cacagtaaga aagcactgat gcgctacgaa gacgtttaca tgcctgaggt
     3481 gtacaaagcg attaacattg cgcaaaacac cgcatggaaa atcaacaaga aagtcctagc
     3541 ggtcgccaac gtaatcacca agtggaagca ttgtccggtc gaggacatcc ctgcgattga
     3601 gcgtgaagaa ctcccgatga aaccggaaga catcgacatg aatcctgagg ctctcaccgc
     3661 gtggaaacgt gctgccgctg ctgtgtaccg caaggacaag gctcgcaagt ctcgccgtat
     3721 cagccttgag ttcatgcttg agcaagccaa taagtttgct aaccataagg ccatctggtt
     3781 cccttacaac atggactggc gcggtcgtgt ttacgctgtg tcaatgttca acccgcaagg
     3841 taacgatatg accaaaggac tgcttacgct ggcgaaaggt aaaccaatcg gtaaggaagg
     3901 ttactactgg ctgaaaatcc acggtgcaaa ctgtgcgggt gtcgataagg ttccgttccc
     3961 tgagcgcatc aagttcattg aggaaaacca cgagaacatc atggcttgcg ctaagtctcc
     4021 actggagaac acttggtggg ctgagcaaga ttctccgttc tgcttccttg cgttctgctt
     4081 tgagtacgct ggggtacagc accacggcct gagctataac tgctcccttc cgctggcgtt
     4141 tgacgggtct tgctctggca tccagcactt ctccgcgatg ctccgagatg aggtaggtgg
     4201 tcgcgcggtt aacttgcttc ctagtgaaac cgttcaggac atctacggga ttgttgctaa
     4261 gaaagtcaac gagattctac aagcagacgc aatcaatggg accgataacg aagtagttac
     4321 cgtgaccgat gagaacactg gtgaaatctc tgagaaagtc aagctgggca ctaaggcact
     4381 ggctggtcaa tggctggctt acggtgttac tcgcagtgtg actaagcgtt cagtcatgac
     4441 gctggcttac gggtccaaag agttcggctt ccgtcaacaa gtgctggaag ataccattca
     4501 gccagctatt gattccggca agggtctgat gttcactcag ccgaatcagg ctgctggata
     4561 catggctaag ctgatttggg aatctgtgag cgtgacggtg gtagctgcgg ttgaagcaat
     4621 gaactggctt aagtctgctg ctaagctgct ggctgctgag gtcaaagata agaagactgg
     4681 agagattctt cgcaagcgtt gcgctgtgca ttgggtaact cctgatggtt tccctgtgtg
     4741 gcaggaatac aagaagccta ttcagacgcg cttgaacctg atgttcctcg gtcagttccg
     4801 cttacagcct accattaaca ccaacaaaga tagcgagatt gatgcacaca aacaggagtc
     4861 tggtatcgct cctaactttg tacacagcca agacggtagc caccttcgta agactgtagt
     4921 gtgggcacac gagaagtacg gtatcgaatc ttttgcactg attcacgact ccttcggtac
     4981 cattccggct gacgctgcga acctgttcaa agcagtgcgc gaaactatgg ttgacacata
     5041 tgagtcttgt gatgtactgg ctgatttcta cgaccagttc gctgaccagt tgcacgagtc
     5101 tcaattggac aaaatgccag cacttccggc taaaggtaac ttgaacctcc gtgacatctt
     5161 agagtccgac ttcgcgttcg cctaatggc
//
