LOCUS       Exported                5186 bp ds-DNA     circular SYN 13-NOV-2019
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...8q-b7fdfaq
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 2, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(828..1019)
                     /standard_name="Rop"
                     /label=Rop
                     /note="Geneious type: rep_origin"
     modified_base   1115..1129
     terminator      1130..1174
     primer_bind     1170..1193
                     /standard_name="BT-120SF"
                     /label=BT-120SF
     misc_feature    1188..1196
                     /standard_name="USER junction"
                     /label=USER junction
     misc_feature    1197..1369
                     /standard_name="insulated proA"
                     /label=insulated proA
                     /note="Geneious type: promoter"
     promoter        1259..1264
     misc_feature    1262..1269
                     /standard_name="USER Junction"
                     /label=USER Junction
     promoter        1282..1287
     prim_transcript 1294
                     /standard_name="+1 TSS"
                     /label=+1 TSS
     misc_feature    1369^1370
                     /standard_name="AGT"
                     /label=AGT
                     /note="Geneious type: Editing History Deletion"
     RBS             1370..1384
     misc_feature    1385..4036
                     /standard_name="T7 RNAP"
                     /label=T7 RNAP
                     /note="Geneious type: CDS"
     misc_feature    complement(1537..1539)
                     /standard_name="PAM"
                     /label=PAM
     misc_feature    complement(1540..1559)
                     /standard_name="gRNA"
                     /label=gRNA
                     /note="Geneious type: misc_RNA"
     misc_feature    complement(1552..1554)
                     /standard_name="CAC excellent site"
                     /label=CAC excellent site
     misc_feature    1553..1558
                     /standard_name="RQ"
                     /label=RQ
                     /note="Geneious type: marker"
     misc_feature    complement(1553..1556)
                     /standard_name="window 4-7"
                     /label=window 4-7
     misc_feature    complement(1555..1557)
                     /standard_name="TAT excellent site"
                     /label=TAT excellent site
     misc_feature    4017..4035
                     /standard_name="prime"
                     /label=prime
     misc_feature    4034..4037
                     /standard_name="TAAT*"
                     /label=TAAT*
     misc_feature    4041..4063
                     /standard_name="primer"
                     /label=primer
     terminator      complement(4051..4092)
     modified_base   4093..4105
     misc_feature    complement(4095..4115)
                     /standard_name="BT-120SR"
                     /label=BT-120SR
                     /note="Geneious type: primer_bind_reverse"
     CDS             complement(4106..4885)
                     /standard_name="aadA"
                     /label=aadA
     promoter        4898..4982
     misc_feature    4954
                     /standard_name="G>T vs original map"
                     /label=G>T vs original map
     rep_origin      join(4998..5186,1..400)
                     /direction=RIGHT
     misc_feature    4998
                     /standard_name="T deletion"
                     /label=T deletion
ORIGIN
        1 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
       61 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
      121 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
      181 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
      241 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
      301 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
      361 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
      421 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
      481 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
      541 aacgaccgag cgcagcgagt cagtgagcga ggaagcggat gagcgcctga tgcggtattt
      601 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
      661 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
      721 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
      781 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
      841 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc
      901 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt
      961 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact
     1021 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag
     1081 aggatgctca cgatacgggt tactgatgat gaacaagagg acatccggtc aaataaaacg
     1141 aaaggctcag tcgaaagact gggcctttcg ttttgctgag gagacttagg gactctcaca
     1201 gctaacacca cgtcgtccct atctgctgcc ctaggtctat gagtggttgc tggataactt
     1261 tacgggcatg cataaggctc gtaggctata ttcagggaga ctacaacggt ttccctctac
     1321 aaataatttt gtttaacttt tactagagtg ggaccctacc tgcaggtgca aggaggaaaa
     1381 aaaaatgaac acgattaaca tcgctaagaa cgacttctct gacatcgaac tggctgctat
     1441 cccgttcaac actctggctg accattacgg tgagcgttta gctcgcgaac agttggccct
     1501 tgagcatgag tcttacgaga tgggtgaagc acgcttccgc aagatgtttg agtgataact
     1561 taaagctggt gaggttgcgg ataacgctgc cgccaagcct ctcatcacta ccctactccc
     1621 taagatgatt gcacgcatca acgactggtt tgaggaagtg aaagctaagc gcggcaagcg
     1681 cccgacagcc ttccagttcc tgcaagaaat caagccggaa gccgtagcgt acatcaccat
     1741 taagaccact ctggcttgcc taaccagtgc tgacaataca accgttcagg ctgtagcaag
     1801 cgcaatcggt cgggccattg aggacgaggc tcgcttcggt cgtatccgtg accttaaagc
     1861 taagcacttc aagaaaaacg ttgaggaaca actcaacaag cgcgtagggc acgtctacaa
     1921 gaaagcattt atgcaagttg tcgaggctga catgctctct aagggtctac tcggtggcga
     1981 ggcgtggtct tcgtggcata aggaagactc tattcatgta ggagtacgct gcatcgagat
     2041 gctcattgag tcaaccggaa tggttagctt acaccgccaa aatgctggcg tagtaggtca
     2101 agactctgag actatcgaac tcgcacctga atacgctgag gctatcgcaa cccgtgcagg
     2161 tgcgctggct ggcatctctc cgatgttcca accttgcgta gttcctccta agccgtggac
     2221 tggcattact ggtggtggct attgggctaa cggtcgtcgt cctctggcgc tggtgcgtac
     2281 tcacagtaag aaagcactga tgcgctacga agacgtttac atgcctgagg tgtacaaagc
     2341 gattaacatt gcgcaaaaca ccgcatggaa aatcaacaag aaagtcctag cggtcgccaa
     2401 cgtaatcacc aagtggaagc attgtccggt cgaggacatc cctgcgattg agcgtgaaga
     2461 actcccgatg aaaccggaag acatcgacat gaatcctgag gctctcaccg cgtggaaacg
     2521 tgctgccgct gctgtgtacc gcaaggacaa ggctcgcaag tctcgccgta tcagccttga
     2581 gttcatgctt gagcaagcca ataagtttgc taaccataag gccatctggt tcccttacaa
     2641 catggactgg cgcggtcgtg tttacgctgt gtcaatgttc aacccgcaag gtaacgatat
     2701 gaccaaagga ctgcttacgc tggcgaaagg taaaccaatc ggtaaggaag gttactactg
     2761 gctgaaaatc cacggtgcaa actgtgcggg tgtcgataag gttccgttcc ctgagcgcat
     2821 caagttcatt gaggaaaacc acgagaacat catggcttgc gctaagtctc cactggagaa
     2881 cacttggtgg gctgagcaag attctccgtt ctgcttcctt gcgttctgct ttgagtacgc
     2941 tggggtacag caccacggcc tgagctataa ctgctccctt ccgctggcgt ttgacgggtc
     3001 ttgctctggc atccagcact tctccgcgat gctccgagat gaggtaggtg gtcgcgcggt
     3061 taacttgctt cctagtgaaa ccgttcagga catctacggg attgttgcta agaaagtcaa
     3121 cgagattcta caagcagacg caatcaatgg gaccgataac gaagtagtta ccgtgaccga
     3181 tgagaacact ggtgaaatct ctgagaaagt caagctgggc actaaggcac tggctggtca
     3241 atggctggct tacggtgtta ctcgcagtgt gactaagcgt tcagtcatga cgctggctta
     3301 cgggtccaaa gagttcggct tccgtcaaca agtgctggaa gataccattc agccagctat
     3361 tgattccggc aagggtctga tgttcactca gccgaatcag gctgctggat acatggctaa
     3421 gctgatttgg gaatctgtga gcgtgacggt ggtagctgcg gttgaagcaa tgaactggct
     3481 taagtctgct gctaagctgc tggctgctga ggtcaaagat aagaagactg gagagattct
     3541 tcgcaagcgt tgcgctgtgc attgggtaac tcctgatggt ttccctgtgt ggcaggaata
     3601 caagaagcct attcagacgc gcttgaacct gatgttcctc ggtcagttcc gcttacagcc
     3661 taccattaac accaacaaag atagcgagat tgatgcacac aaacaggagt ctggtatcgc
     3721 tcctaacttt gtacacagcc aagacggtag ccaccttcgt aagactgtag tgtgggcaca
     3781 cgagaagtac ggtatcgaat cttttgcact gattcacgac tccttcggta ccattccggc
     3841 tgacgctgcg aacctgttca aagcagtgcg cgaaactatg gttgacacat atgagtcttg
     3901 tgatgtactg gctgatttct acgaccagtt cgctgaccag ttgcacgagt ctcaattgga
     3961 caaaatgcca gcacttccgg ctaaaggtaa cttgaacctc cgtgacatct tagagtccga
     4021 cttcgcgttc gcctaatggc actcctcagc aaatataatg accctcttga taacccaaga
     4081 gggcattttt taatgcccat ggcgtttatt tgccgactac cttggtgatc tcgcctttca
     4141 cgtagtggac aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc
     4201 caagataagc ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca
     4261 ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa
     4321 tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc
     4381 atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga
     4441 gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga
     4501 tagccagatc aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct
     4561 gccattctcc aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt
     4621 gcacaacaat ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag
     4681 tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg
     4741 taaccagcaa atcaatatca ctgtgtggct tcaggccgcc atccactgcg gagccgtaca
     4801 aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata
     4861 gttgagtcga tacttcggcg atcaccgctt ccctcatact cttccttttt caatattatt
     4921 gaagcattta tcagggttat tgtctcatga gcgtatacat atttgaatgt atttagaaaa
     4981 ataggccaaa taggccgttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5041 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc acctcttttt
     5101 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     5161 tagttaggcc accacttcaa gaactc
//
