LOCUS       pET-28a(+)-NatL2        6622 bp    DNA     circular      9-JUL-2018
SOURCE      
  ORGANISM  
COMMENT     http://www.informaxinc.com
            
            http://www.informaxinc.com/
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12369185|
COMMENT     VNTDBDATE|-12369185|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pET-28a(+)-NatL2|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|Larissa Wiskowski|
COMMENT     VNTOAUTHORTEL|608-238-6110|
COMMENT     VNTOAUTHORFAX|608-238-1388|
COMMENT     VNTOAUTHOREML|Novatech@novagen.com|
COMMENT     VNTOAUTHORWWW|www.novagen.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "Currently local object. Original author: Larissa Wiskowski" 
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FEATURES             Location/Qualifiers
     promoter        complement(1623..1639)
                     /vntifkey="30"
                     /label=T7\promoter
     protein_bind    complement(1523..1540)
                     /vntifkey="31"
                     /label=His\tag
     protein_bind    complement(1490..1492)
                     /vntifkey="31"
                     /label=T7\tag
     protein_bind    complement(140..157)
                     /vntifkey="31"
                     /label=His\tag
     terminator      complement(26..72)
                     /vntifkey="43"
                     /label=T7\terminator
     CDS             2026..3105
                     /vntifkey="4"
                     /label=lac\I
     rep_origin      complement(4539..4539)
                     /vntifkey="33"
                     /label=ColE1\pBR322\origin
     CDS             5248..6060
                     /vntifkey="4"
                     /label=kan\sequence
     rep_origin      complement(6156..6611)
                     /vntifkey="33"
                     /label=f1\origin
     CDS             complement(1596..1620)
                     /vntifkey="4"
                     /label=lac\operator
     protein_bind    complement(1496..1513)
                     /vntifkey="31"
                     /label=thrombin
     misc_feature    complement(179..1489)
                     /vntifkey="21"
                     /label=NatL2
BASE COUNT     1428 a      1811 c      1944 g      1439 t 
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagctttc 
      181 agaagtacat gaccgccttg tcccagtcct cgtcctccgc gaagaggtag cgggccttga 
      241 ggatgtcgcg ctgggcggtg agggcctccg ccgggacgag ggtgcccggg gcgaggccgg 
      301 tgatgcggtg cgggaccccc agttcgcggt cgagcgcggc gccgagctcc ttgacggccc 
      361 gctgccgcac cggctcgggc gcctcgaact ccagctccag cacgtccggg tgggccttgg 
      421 cccgccagaa catcacctcg tacgcggccg gcagggagaa caccagctcc tccagccgct 
      481 gctgggtgac ggtcgccgga ccgatccggt gaccggttcc ggcccgcccc agcacggtga 
      541 ccgtgggcag cagccagccg cagccgcacg ggtcggtgga cacctcgacg tcgtcggcga 
      601 ggttgtagcg cagcagcggc atcgcgtccc ggtacagcgg ggtgacgacc atctggccgc 
      661 ggcccgcctc ggagagcgtg ccggtcctcg ggtcgtacac ctcgaagatc gcccggtccg 
      721 cccacaggtg catccggccc tcggggcact gcccggcgat ggtgccggtc tccgtggagc 
      781 cgtactcctc gacgaccggg atgccgcccc agatctcgct gagccggcgg cgccgcacct 
      841 cggtcagcgg ctcggcggcg gtgaacatgg cccgcaggtg cgggaagtcc tggtcggggc 
      901 ggagtccggc ggccttggcg gcggcggcca gcatggtgat ctcggtcggg ttgcaccagg 
      961 tgagcgtcac gtcgagggtc ttcaggacgc gcaccatgcg ggagagcggg gtggccagcg 
     1021 agcgggcgtc gccggggacg acggtcgcac cgcgcagccg tcccgcggcc tgggcgaggt 
     1081 ggccggtgat gaccaggccg tagggggtgc gcaccaggaa ggtgtcggag gggtggatgc 
     1141 cggtccactt gcgggcgaag cgctcggcga ggtcggtcca gtcctcctcc gtgtagtagg 
     1201 aggcggtggg ttcgcccgcg gtgccgctgg actcgtggta ggtggccagg tgttcgcgcc 
     1261 cgaccgcgag catcccgaag gggtactggt cgcgcaggtc ctgcttggcg gtcacctcga 
     1321 cgccggcgaa gtcgtccgcg gtgcgcggcg gtgtggtgcc gcggtagcgg gccgcgtaga 
     1381 acggcgaacg gagtgcctgc gcgaggaccc tcggcagctg ggaccgctgg agctcggcca 
     1441 gttcggccgg gctcgaccag tcaccaagtt ccggacgcga gcgcgacatc atatggctgc 
     1501 cgcgcggcac caggccgctg ctgtgatgat gatgatgatg gctgctgccc atggtatatc 
     1561 tccttcttaa agttaaacaa aattatttct agaggggaat tgttatccgc tcacaattcc 
     1621 cctatagtga gtcgtattaa tttcgcggga tcgagatctc gatcctctac gccggacgca 
     1681 tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc gccgacatca 
     1741 ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc ggcgtgggta 
     1801 tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat gcaccattcc 
     1861 ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta atgcaggagt 
     1921 cgcataaggg agagcgtcga gatcccggac accatcgaat ggcgcaaaac ctttcgcggt 
     1981 atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg 
     2041 ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac 
     2101 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg 
     2161 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc 
     2221 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct 
     2281 cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa 
     2341 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac 
     2401 tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg 
     2461 ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac 
     2521 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta 
     2581 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc 
     2641 actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt 
     2701 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc 
     2761 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt 
     2821 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg 
     2881 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg 
     2941 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa 
     3001 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca 
     3061 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat 
     3121 taatgtaagt tagctcactc attaggcacc gggatctcga ccgatgccct tgagagcctt 
     3181 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac 
     3241 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg 
     3301 cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat 
     3361 cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa 
     3421 gcaggccatt atcgccggca tggcggcccc acgggtgcgc atgatcgtgc tcctgtcgtt 
     3481 gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg 
     3541 cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat 
     3601 ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat 
     3661 tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac ctacatctgt 
     3721 attaacgaag cgctggcatt gaccctgagt gatttttctc tggtcccgcc gcatccatac 
     3781 cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat cagtaacccg 
     3841 tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca gaaatccccc 
     3901 ttacacggag gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat 
     3961 cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc 
     4021 agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg 
     4081 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 
     4141 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 
     4201 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac 
     4261 tatgcggcat cagagcagat tgtactgaga gtgcaccata tatgcggtgt gaaataccgc 
     4321 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact 
     4381 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 
     4441 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 
     4501 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 
     4561 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 
     4621 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 
     4681 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 
     4741 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 
     4801 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 
     4861 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 
     4921 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 
     4981 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 
     5041 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 
     5101 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 
     5161 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gaacaataaa actgtctgct 
     5221 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctct 
     5281 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat 
     5341 aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag 
     5401 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga 
     5461 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct 
     5521 gatgatgcat ggttactcac cactgcgatc cccgggaaaa cagcattcca ggtattagaa 
     5581 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg 
     5641 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag 
     5701 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat 
     5761 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat 
     5821 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta 
     5881 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc 
     5941 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat 
     6001 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc 
     6061 taagaattaa ttcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 
     6121 ggttccgcgc acatttcccc gaaaagtgcc acctgaaatt gtaaacgtta atattttgtt 
     6181 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg 
     6241 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg 
     6301 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta 
     6361 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg 
     6421 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa 
     6481 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct 
     6541 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct 
     6601 acagggcgcg tcccattcgc ca 
//
