LOCUS       Exported                9837 bp ds-DNA     circular SYN 14-JAN-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9837)
  AUTHORS   Rob Mitra Lab WUSTL
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jan 16, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9837
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..502
                     /label=ADH1 Promoter
                     /note="ApEinfo_fwdcolor=#4d40bf
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     promoter        1..298
                     /label=ADH1 promoter
                     /note="ApEinfo_fwdcolor=#0080c0
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#0080c0"
     misc_feature    472..492
                     /label=sequencing forward primer OM6189
                     /note="ApEinfo_fwdcolor=#8eff41
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    497..535
                     /label=Forwrd linker
                     /note="ApEinfo_fwdcolor=#ea1aff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    536..1378
                     /label=GCN4
     misc_feature    1379..2128
                     /label=Sir4-CTD
                     /note="ApEinfo_fwdcolor=#fff672
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    1379..1417
                     /label=Reverse Linker
                     /note="ApEinfo_fwdcolor=#d8ffa4
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    1485..1508
                     /label=OM6373-long
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    1485..1504
                     /label=sequencing reverse primer OM6373
                     /note="ApEinfo_fwdcolor=#24ff25
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    2129..2269
                     /label=3XMyc
                     /note="ApEinfo_fwdcolor=#965cff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     CDS             2975..3043
                     /label=LacZ alpha
                     /note="ApEinfo_fwdcolor=#6495ed
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#6495ed"
     misc_feature    3101..3953
                     /label=CEN
                     /note="ApEinfo_fwdcolor=cyan
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    5078..5274
                     /label=LEU2 ORF
                     /note="ApEinfo_fwdcolor=#9684ff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=green"
     misc_feature    5277..5966
                     /label=LEU2 ORF(1)
                     /note="ApEinfo_fwdcolor=#9684ff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=LEU2 ORF
                     ApEinfo_revcolor=green"
     misc_feature    5968..6004
                     /label=LEU2 ORF(2)
                     /note="ApEinfo_fwdcolor=#9684ff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=LEU2 ORF
                     ApEinfo_revcolor=green"
     misc_feature    6006..6172
                     /label=LEU2 ORF(3)
                     /note="ApEinfo_fwdcolor=#9684ff
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_label=LEU2 ORF
                     ApEinfo_revcolor=green"
     CDS             7231..7890
                     /label=AmpR
                     /note="ApEinfo_fwdcolor=yellow
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=yellow"
     rep_origin      7988..8670
                     /label=ColE1 origin
                     /note="ApEinfo_fwdcolor=gray50
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=gray50"
     promoter        8894..9837
                     /label=ADH1 promoter
                     /note="ApEinfo_fwdcolor=#0080c0
                     ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0} 
                     width 5 offset 0
                     ApEinfo_revcolor=#0080c0"
ORIGIN
        1 gtggtacata acgaactaat actgtagccc tagacttgat agccatcatc atatcgaagt
       61 ttcactaccc tttttccatt tgccatctat tgaagtaata ataggcgcat gcaacttctt
      121 ttcttttttt ttcttttctc tctcccccgt tgttgtctca ccatatccgc aatgacaaaa
      181 aaatgatgga agacactaaa ggaaaaaatt aacgacaaag acagcaccaa cagatgtcgt
      241 tgttccagag ctgatgaggg gtatctcgaa gcacacgaaa ctttttcctt ccttcattca
      301 cgcacactac tctctaatga gcaacggtat acggccttcc ttccagttac ttgaatttga
      361 aataaaaaaa agtttgctgt cttgctatca agtataaata gacctgcaat tattaatctt
      421 ttgtttcctc gtcattgttc tcgttccctt tcttccttgt ttctttttct gcacaatatt
      481 tcaagctata ccaagcatac aatcaactcc aagcttgaag caagcctcct gaaagatgtc
      541 cgaatatcag ccaagtttat ttgctttaaa tccaatgggt ttctcaccat tggatggttc
      601 taaatcaacc aacgaaaatg tatctgcttc cacttctact gccaaaccaa tggttggcca
      661 attgattttt gataaattca tcaagactga agaggatcca attatcaaac aggatacccc
      721 ttcgaacctt gattttgatt ttgctcttcc acaaacggca actgcacctg atgccaagac
      781 cgttttgcca attccggagc tagatgacgc tgtagtggaa tctttctttt cgtcaagcac
      841 tgattcaact ccaatgtttg agtatgaaaa cctagaagac aactctaaag aatggacatc
      901 cttgtttgac aatgacattc cagttaccac tgacgatgtt tcattggctg ataaggcaat
      961 tgaatccact gaagaagttt ctctggtacc atccaatctg gaagtctcga caacttcatt
     1021 cttacccact cctgttctag aagatgctaa actgactcaa acaagaaagg ttaagaaacc
     1081 aaattcagtc gttaagaagt cacatcatgt tggaaaggat gacgaatcga gactggatca
     1141 tctaggtgtt gttgcttaca accgcaaaca gcgttcgatt ccactttctc caattgtgcc
     1201 cgaatccagt gatcctgctg ctctaaaacg tgctagaaac actgaagccg ccaggcgttc
     1261 tcgtgcgaga aagttgcaaa gaatgaaaca acttgaagac aaggttgaag aattgctttc
     1321 gaaaaattat cacttggaaa atgaggttgc cagattaaag aaattagttg gcgaacgcag
     1381 agtgtcgcat agtgatacta attctagcaa acccaaaaac acgaaggaga acctttcaaa
     1441 aagctcttgg aggcaagaat ggcttgcaaa tttgaaactt atttccgttt cgttggttga
     1501 tgagttccct tcggagcttt ccgacagtga tagacaaata attaacgaaa aaatgcagtt
     1561 acttaaagat atatttgcta acaaccttaa atcagcaatt tccaataatt ttagagagag
     1621 tgacatcatt atactgaaag gtgaaataga agattaccca atgagttctg aaattaagat
     1681 ttactacaac gaattacaga acaagcctga tgcaaaaaaa gccagatttt ggtcatttat
     1741 gaagactcag agatttgttt ccaacatggg atttgatatt cagaagtcct gtgaacctgt
     1801 ttctatatcc acgtcggtca aaccacatgt ggtcgagccc gagcatatgg cagatgccaa
     1861 aattatgcct aaggatatac tgcaaattac aaaaaaaccg ttaatggtta aaaatgtgaa
     1921 gccttcttct ccgccagatg tgaagtcttt ggtgcagctg agcacaatgg aaacgaaaac
     1981 cctaccagaa aagaagcaat tcgacagtat ttttaattct aacaaagcaa aaataatccc
     2041 tggaaatggg aagcacgcat cagaaaacat ctcactctct ttctcaagac ctgcgtccta
     2101 cggctatttt tctgttggaa aaagggttcg gggatcctct agaggtgaac aaaagttgat
     2161 ttctgaagaa gatttgaacg gtgaacaaaa gctaatctcc gaggaacact tgaacggtga
     2221 acaaaaatta atctcagaag aagacttgaa cggatcctct agagtcgact gaatcgtaga
     2281 tactgaaaaa ccccgcaagt tcacttcaac tgtgcatcgt gcaccatctc aatttctttc
     2341 atttatacat cgttttgcct tcttttatgt aactatactc ctctaagttt caatcttggc
     2401 catgtaacct ctgatctata gaatttttta aatgactaga attaatgccc atcttttttt
     2461 tggacctaaa ttcttcatga aaatatatta cgagggctta ttcagaagct ttggacttct
     2521 tcgccagagg tttggtcaag tctccaatca aggttgtcgg cttgtctacc ttgccagaaa
     2581 tttacgaaaa gatggaaaag ggtcaaatcg ttggtagata cgttgttgac acttctaaat
     2641 aagcgaattt cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg
     2701 tatacaaatt ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact
     2761 ctttcctgta ggtcaggttg ctttctcagg tatagcatga ggtcgctctt attgaccaca
     2821 cctctaccgg catgccgagc aaatgcctgc aaatcgctcc ccatttcacc caattgtaga
     2881 tatgctaact ccagcaatga gttgatgaat ctcggtgtgt attttatgtc ctcagaggac
     2941 aacacctgtt gtaatcgttc ttccacacgg atcctggcgt aatagcgaag aggcccgcac
     3001 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
     3061 tctccttacg catctgtgcg gtatttcaca ccgcatatat cgctgggcca ttctcatgaa
     3121 gaatatcttg aatttattgt catattacta gttggtgtgg aagtccatat atcggtgatc
     3181 aatatagtgg ttgacatgct ggctagtcaa cattgagcct tttgatcatg caaatatatt
     3241 acggtatttt acaatcaaat atcaaactta actattgact ttataactta tttaggtggt
     3301 aacattctta taaaaaagaa aaaaattact gcaaaacagt actagctttt aacttgtatc
     3361 ctaggttatc tatgctgtct caccatagag aatattacct atttcagaat gtatgtccat
     3421 gattcgccgg gtaaatacat ataatacaca aatctggctt aataaagtct ataatatatc
     3481 tcataaagaa gtgctaaatt ggctagtgct atatattttt aagaaaattt cttttgacta
     3541 agtccatatc gactttgtaa aagttcactt tagcatacat atattacacg agccagaaat
     3601 tgtaactttt gcctaaaatc acaaattgca aaatttaatt gcttgcaaaa ggtcacatgc
     3661 ttataatcaa cttttttaaa aatttaaaat acttttttat tttttatttt taaacataaa
     3721 tgaaataatt tatttattgt ttatgattac cgaaacataa aacctgctca agaaaaagaa
     3781 actgttttgt ccttggaaaa aaagcactac ctaggagcgg ccaaaatgcc gaggctttca
     3841 tagcttaaac tctttacaga aaataggcat tatagatcag ttcgagtttt cttattcttc
     3901 cttccggttt tatcgtcaca gttttacagt aaataagtat cacctcttag agttcgatga
     3961 taagctgtca aacatgagaa ttaattccac atgttaaaat agtgaaggag catgttcggc
     4021 acacagtgga ccgaacgtgg ggtaagtgca ctagggtccg gttaaacgga tctcgcattg
     4081 atgaggcaac gctaattatc aacatataga ttgttatcta tctgcatgaa cacgaaatct
     4141 ttacttgacg acttgaggct gatggtgttt atgcaaagaa accactgtgt ttaatatgtg
     4201 tcactgtttg atattactgt cagcgtagaa gataatagta aaagcggtta ataagtgtat
     4261 ttgagataag tgtgataaag tttttacagc gaaaagacga taaatacaag aaaatgatta
     4321 cgaggatacg gagagaggta tgtacatgtg tatttatata ctaagctgcc ggcggttgtt
     4381 tgcaagaccg agaaaaggct agcaagaatc gggtcattgt agcgtatgcg cctgtgaaca
     4441 ttctcttcaa caagtttgat tccattgcgg tgaaatggta aaagtcaacc ccctgcgatg
     4501 tatattttcc tgtacaatca atcaaaaagc caaatgattt agcattatct ttacatcttg
     4561 ttattttaca gattttatgt ttagatcttt tatgcttgct tttcaaaagg cttgcaaggc
     4621 cgtttctgac agagtaaaat tcttgaggga actttcacca ttatgggaaa tggttcaaga
     4681 aggtattgac ttaaactcca tcaaatggtc aggtcattga gtgtttttta tttgttgtat
     4741 tttttttttt ttagagaaaa tcctccaata ttaaattagg aatcatagtt tcatgatttt
     4801 ctgttacacc taactttttg tgtggtgccc tcctccttgt caatattaat gttaaagtgc
     4861 aattcttttt ccttatcacg ttgagccatt agtatcaatt tgcttacctg tattccttta
     4921 catcctcctt tttctccttc ttgataaatg tatgtagatt gcgtatatag tttcgtctac
     4981 cctatgaaca tattccattt tgtaatttcg tgtcgtttct attatgaatt tcatttataa
     5041 agtttatgta caaatatcat aaaaaaagag aatcttttta agcaaggatt ttcttaactt
     5101 cttcggcgac agcatcaccg acttcggtgg tactgttgga accacctaaa tcaccagttc
     5161 tgatacctgc atccaaaacc tttttaactg catcttcaat ggccttacct tcttcaggca
     5221 agttcaatga caatttcaac atcattgcag cagacaagat agtggcgata gggttgacct
     5281 tattctttgg caaatctgga gcagaaccgt ggcatggttc gtacaaacca aatgcggtgt
     5341 tcttgtctgg caaagaggcc aaggacgcag atggcaacaa acccaaggaa cctgggataa
     5401 cggaggcttc atcggagatg atatcaccaa acatgttgct ggtgattata ataccattta
     5461 ggtgggttgg gttcttaact aggatcatgg cggcagaatc aatcaattga tgttgaacct
     5521 tcaatgtagg gaattcgttc ttgatggttt cctccacagt ttttctccat aatcttgaag
     5581 aggccaaaac attagcttta tccaaggacc aaataggcaa tggtggctca tgttgtaggg
     5641 ccatgaaagc ggccattctt gtgattcttt gcacttctgg aacggtgtat tgttcactat
     5701 cccaagcgac accatcacca tcgtcttcct ttctcttacc aaagtaaata cctcccacta
     5761 attctctgac aacaacgaag tcagtacctt tagcaaattg tggcttgatt ggagataagt
     5821 ctaaaagaga gtcggatgca aagttacatg gtcttaagtt ggcgtacaat tgaagttctt
     5881 tacggatttt tagtaaacct tgttcaggtc taacactacc ggtaccccat ttaggaccac
     5941 ccacagcacc taacaaaacg gcatcagcct tcttggaggc ttccagcgcc tcatctggaa
     6001 gtggaacacc tgtagcatcg atagcagcac caccaattaa atgattttcg aaatcgaact
     6061 tgacattgga acgaacatca gaaatagctt taagaacctt aatggcttcg gctgtgattt
     6121 cttgaccaac gtggtcacct ggcaaaacga cgatcttctt aggggcagac attagaatgg
     6181 tatatccttg aaatatatat atatatattg ctgaaatgta aaaggtaaga aaagttagaa
     6241 agtaagacga ttgctaacca cctattggaa aaaacaatag gtccttaaat aatattgtca
     6301 acttcaagta ttgtgatgca agcatttagt catgaacgct tctctattct atatgaaaag
     6361 ccggttccgg cgctctcacc tttccttttt ctcccaattt ttcagttgaa aaaggtatat
     6421 gcgtcaggcg acctctgaaa ttaacaaaaa atttccagtc atcgaatttg attctgtgcg
     6481 atagcgcccc tgtgtgttct cgttatgttg aggaaaaaaa taatggttgc taagagattc
     6541 gaactcttgc atcttacgat acctgagtat tcccacagtt aactgcggtc aagatatttc
     6601 ttgaatcagg cgccttagac cgctcggcca aacaaccaat tacttgttga gaaatagagt
     6661 ataattatcc tataaatata acgtttttga acacacatga acaaggaagt acaggacaat
     6721 tgattttgaa gagaatgtgg attttgatgt aattgttggg attccatttt taataaggca
     6781 ataatattag gtatgtagat atactagaag ttctcctcga ggaattcttg aagacgaaag
     6841 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     6901 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     6961 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7021 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     7081 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7141 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7201 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7261 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7321 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7381 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     7441 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     7501 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     7561 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     7621 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     7681 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     7741 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     7801 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     7861 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     7921 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     7981 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8041 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8101 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8161 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     8221 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8281 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8341 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8401 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     8461 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8521 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8581 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     8641 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     8701 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     8761 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     8821 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     8881 taatgcagga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc aaggagcatg
     8941 aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt tgatatgatg
     9001 tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca tgctgactct
     9061 gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc tgtgcccggc
     9121 ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg agattggaga
     9181 agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg gtgtcattat
     9241 ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa gaatgagcca
     9301 agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat gaccttgaag
     9361 gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg gttgctacca
     9421 gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt acgctttcaa
     9481 ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac acttctccta
     9541 tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac ccctccgcgc
     9601 tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt tgtttccggg
     9661 tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga ttcctataat
     9721 accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat agaacagata
     9781 ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc attgatg
//
