LOCUS       pACDura3(s10)-C-        7264 bp    DNA     circular     09-JAN-2020
pACD4K-C Vector Sequence (7,675 bp)
N's are variable positions introduced during primer-based mutagenesis of the 350-bp fragment (between HindIII-BsrGI) for gene-specific targeting.
FEATURES             Location/Qualifiers
     misc_feature    585..1497
                     /note="p15A ori"
     promoter        1741..1762
                     /note="T7 promoter"
     misc_feature    1802..1826
                     /note="5' exon IBS"
     misc_feature    1827..3704
                     /note="intron RNA"
     misc_feature    2102..2111
                     /note="EBS1d"
     misc_feature    3705..3714
                     /note="3' exon"
     misc_difference 3951..5750
                     /note="LtrA ORF"
     misc_feature    5886..6176
                     /note="T1/T2 transcriptional terminator"
     gene            complement(join(6827..7264,1..219))
                     /note="Cm resistance gene"
     gene            6114..6141
                     /note=""
     gene            5939..5966
                     /note=""
     misc_feature    2652..2659
                     /note="RBS"
     misc_feature    2660..2666
                     /note="Linker"
     insert          2518..2589
                     /source="PEM7-Promoter.seq"
     misc_feature    2667..3470
                     /note="ura3 (optimized)"
     misc_feature    2046..2054
                     /note="EBS2"
     source          1..7264
                     /dnas_title="pACDura3(s10)-C-635s-retargeted features"
ORIGIN      
        1 GAATTCCGGA TGAGCATTCA TCAGGCGGGC AAGAATGTGA ATAAAGGCCG GATAAAACTT
       61 GTGCTTATTT TTCTTTACGG TCTTTAAAAA GGCCGTAATA TCCAGCTGAA CGGTCTGGTT
      121 ATAGGTACAT TGAGCAACTG ACTGAAATGC CTCAAAATGT TCTTTACGAT GCCATTGGGA
      181 TATATCAACG GTGGTATATC CAGTGATTTT TTTCTCCATT TTAGCTTCCT TAGCTCCTGA
      241 AAATCTCGAT AACTCAAAAA ATACGCCCGG TAGTGATCTT ATTTCATTAT GGTGAAAGTT
      301 GGAACCTCTT ACGTGCCGAT CAACGTCTCA TTTTCGCCAA AAGTTGGCCC AGGGCTTCCC
      361 GGTATCAACA GGGACACCAG GATTTATTTA TTCTGCGAAG TGATCTTCCG TCACAGGTAT
      421 TTATTCGGCG CAAAGTGCGT CGGGTGATGC TGCCAACTTA CTGATTTAGT GTATGATGGT
      481 GTTTTTGAGG TGCTCCAGTG GCTTCTGTTT CTATCAGCTG TCCCTCCTGT TCAGCTACTG
      541 ACGGGGTGGT GCGTAACGGC AAAAGCACCG CCGGACATCA GCGCTAGCGG AGTGTATACT
      601 GGCTTACTAT GTTGGCACTG ATGAGGGTGT CAGTGAAGTG CTTCATGTGG CAGGAGAAAA
      661 AAGGCTGCAC CGGTGCGTCA GCAGAATATG TGATACAGGA TATATTCCGC TTCCTCGCTC
      721 ACTGACTCGC TACGCTCGGT CGTTCGACTG CGGCGAGCGG AAATGGCTTA CGAACGGGGC
      781 GGAGATTTCC TGGAAGATGC CAGGAAGATA CTTAACAGGG AAGTGAGAGG GCCGCGGCAA
      841 AGCCGTTTTT CCATAGGCTC CGCCCCCCTG ACAAGCATCA CGAAATCTGA CGCTCAAATC
      901 AGTGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGCGGCTCCC
      961 TCGTGCGCTC TCCTGTTCCT GCCTTTCGGT TTACCGGTGT CATTCCGCTG TTATGGCCGC
     1021 GTTTGTCTCA TTCCACGCCT GACACTCAGT TCCGGGTAGG CAGTTCGCTC CAAGCTGGAC
     1081 TGTATGCACG AACCCCCCGT TCAGTCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT
     1141 GAGTCCAACC CGGAAAGACA TGCAAAAGCA CCACTGGCAG CAGCCACTGG TAATTGATTT
     1201 AGAGGAGTTA GTCTTGAAGT CATGCGCCGG TTAAGGCTAA ACTGAAAGGA CAAGTTTTGG
     1261 TGACTGCGCT CCTCCAAGCC AGTTACCTCG GTTCAAAGAG TTGGTAGCTC AGAGAACCTT
     1321 CGAAAAACCG CCCTGCAAGG CGGTTTTTTC GTTTTCAGAG CAAGAGATTA CGCGCAGACC
     1381 AAAACGATCT CAAGAAGATC ATCTTATTAA TCAGATAAAA TATTTCTAGC TAGATTTCAG
     1441 TGCAATTTAT CTCTTCAAAT GTAGCACCTG AAGTCAGCCC CATACGATAT AAGTTGTAAT
     1501 TCTCATGTTT GACAGCTTAT CATCGATAAG CTCAAGGAGA TGGCGCCCAA CAGTCCCCCG
     1561 GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA
     1621 GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG
     1681 CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT
     1741 TAATACGACT CACTATAGGG GAATTGTGAG CGGATAACAA TTCCCCTCTA GAAAAAAGCT
     1801 TATAATTATC CTTACATTTC CCGTGAGTGC GCCCAGATAG GGTGTTAAGT CAAGTAGTTT
     1861 AAGGTACTAC TCTGTAAGAT AACACAGAAA ACAGCCAACC TAACCGAAAA GCGAAAGCTG
     1921 ATACGGGAAC AGAGCACGGT TGGAAAGCGA TGAGTTACCT AAAGACAATC GGGTACGACT
     1981 GAGTCGCAAT GTTAATCAGA TATAAGGTAT AAGTTGTGTT TACTGAACGC AAGTTTCTAA
     2041 TTTCGGTTAA ATGTCGATAG AGGAAAGTGT CTGAAACCTC TAGTACAAAG AAAGGTAAGT
     2101 TACGTCACGG GACTTATCTG TTATCACCAC ATTTGTACAA TCTGTAGGAG AACCTATGGG
     2161 AACGAAACGA AAGCGATGCC GAGAATCTGA ATTTACCAAG ACTTAACACT AACTGGGGAT
     2221 ACCCTAAACA AGAATGCCTA ATAGAAAGGA GGAAAAAGGC TATAGCACTA GAGCTTGAAA
     2281 ATCTTGCAAG GGTACGGAGT ACTCGTAGTA GTCTGAGAAG GGTAACGCCC TTTACATGGC
     2341 AAAGGGGTAC AGTTATTGTG TACTAAAATT AAAAATTGAT TAGGGAGGAA AACCTCAAAA
     2401 TGAAACCAAC AATGGCAATT TTAGAAAGAA TCAGTAAAAA TTCACAAGAA AATATAGACG
     2461 AAGTTTTTAC AAGACTTTAT CGTTATCTTT TACGTCCAGA TATTTATTAC GTGGCGAcgc
     2521 gtTGTTGACA ATTAATCATC GGCATAGTAT ATCGGCATAG TATAATACGA CAAGGTGAGG
     2581 AACTAAACCC ctacgtagag gatcgagatc tcgatcccgc gaaatgAGAC CACAACGGTT
     2641 TCCCTAAagt taAGGAGGAA AAACATATGT CCAAGGCTAC TTACAAGGAG CGTGCTGCTA
     2701 CCCACCCTTC TCCGGTTGCT GCAAAACTGT TCAACATTAT GCACGAGAAG CAGACGAACC
     2761 TGTGCGCTTC CCTGGATGTC CGTACTACTA AGGAGCTGCT GGAGCTGGTT GAAGCACTGG
     2821 GTCCGAAAAT TTGCCTGCTG AAAACTCACG TAGACATCCT GACTGACTTC AGCATGGAAG
     2881 GTACCGTCAA ACCGCTGAAA GCCCTGAGCG CAAAATACAA CTTCCTGCTG TTCGAAGACC
     2941 GTAAGTTCGC TGACATCGGT AACACCGTGA AACTGCAGTA CAGCGCGGGT GTGTACCGTA
     3001 TCGCCGAATG GGCAGACATC ACGAACGCAC ATGGTGTGGT GGGTCCAGGT ATCGTTTCTG
     3061 GTCTGAAACA GGCAGCTGAA GAAGTGACCA AAGAACCGCG TGGTCTGCTG ATGCTGGCGG
     3121 AACTGTCTTG TAAAGGTTCC CTGGCGACCG GTGAATATAC CAAAGGTACC GTTGACATCG
     3181 CGAAATCCGA CAAAGACTTC GTTATTGGCT TTATCGCGCA GCGTGACATG GGCGGCCGTG
     3241 ACGAAGGTTA CGATTGGCTG ATTATGACGC CGGGTGTTGG TCTGGATGAT AAAGGCGATG
     3301 CCCTGGGCCA ACAATACCGT ACCGTAGATG ATGTAGTTTC TACCGGCTCT GATATTATCA
     3361 TCGTTGGCCG CGGCCTGTTT GCCAAAGGCC GCGATGCGAA AGTAGAAGGC GAACGTTATC
     3421 GCAAAGCGGG CTGGGAAGCG TATCTGCGCC GCTGTGGCCA GCAGAATTAA TaaACTtgca
     3481 CGCGTTGGGA AATGGCAATG ATAGCGAAAC AACGTAAAAC TCTTGTTGTA TGCTTTCATT
     3541 GTCATCGTCA CGTGATTCAT AAACACAAGT GAATGTCGAC AGTGAATTTT TACGAACGAA
     3601 CAATAACAGA GCCGTATACT CCGAGAGGGG TACGTACGGT TCCCGAAGAG GGTGGTGCAA
     3661 ACCAGTCACA GTAATGTGAA CAAGGCGGTA CCTCCCTACT TCACCATATC ATTTTCTGCA
     3721 GCCCCCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACATATAT GGCTAGATCG
     3781 TCCATTCCGA CAGCATCGCC AGTCACTATG GCGTGCTGCT AGCGCTATAT GCGTTGATGC
     3841 AATTTCTATG CACTCGTAGT AGTCTGAGAA GGGTAACGCC CTTTACATGG CAAAGGGGTA
     3901 CAGTTATTGT GTACTAAAAT TAAAAATTGA TTAGGGAGGA AAACCTCAAA ATGAAACCAA
     3961 CAATGGCAAT TTTAGAAAGA ATCAGTAAAA ATTCACAAGA AAATATAGAC GAAGTTTTTA
     4021 CAAGACTTTA TCGTTATCTT TTACGTCCAG ATATTTATTA CGTGGCGTAT CAAAATTTAT
     4081 ATTCCAATAA AGGAGCTTCC ACAAAAGGAA TATTAGATGA TACAGCGGAT GGCTTTAGTG
     4141 AAGAAAAAAT AAAAAAGATT ATTCAATCTT TAAAAGACGG AACTTACTAT CCTCAACCTG
     4201 TACGAAGAAT GTATATTGCA AAAAAGAATT CTAAAAAGAT GAGACCTTTA GGAATTCCAA
     4261 CTTTCACAGA TAAATTGATC CAAGAAGCTG TGAGAATAAT TCTTGAATCT ATCTATGAAC
     4321 CGGTATTCGA AGATGTGTCT CACGGTTTTA GACCTCAACG AAGCTGTCAC ACAGCTTTGA
     4381 AAACAATCAA AAGAGAGTTT GGCGGCGCAA GATGGTTTGT GGAGGGAGAT ATAAAAGGCT
     4441 GCTTCGATAA TATAGACCAC GTTACACTCA TTGGACTCAT CAATCTTAAA ATCAAAGATA
     4501 TGAAAATGAG CCAATTGATT TATAAATTTC TAAAAGCAGG TTATCTGGAA AACTGGCAGT
     4561 ATCACAAAAC TTACAGCGGA ACACCTCAAG GTGGAATTCT ATCTCCTCTT TTGGCCAACA
     4621 TCTATCTTCA TGAATTGGAT AAGTTTGTTT TACAACTCAA AATGAAGTTT GACCGAGAAA
     4681 GTCCAGAAAG AATAACACCT GAATATCGGG AGCTCCACAA TGAGATAAAA AGAATTTCTC
     4741 ACCGTCTCAA GAAGTTGGAG GGTGAAGAAA AAGCTAAAGT TCTTTTAGAA TATCAAGAAA
     4801 AACGTAAAAG ATTACCCACA CTCCCCTGTA CCTCACAGAC AAATAAAGTA TTGAAATACG
     4861 TCCGGTATGC GGACGACTTC ATTATCTCTG TTAAAGGAAG CAAAGAGGAC TGTCAATGGA
     4921 TAAAAGAACA ATTAAAACTT TTTATTCATA ACAAGCTAAA AATGGAATTG AGTGAAGAAA
     4981 AAACACTCAT CACACATAGC AGTCAACCCG CTCGTTTTCT GGGATATGAT ATACGAGTAA
     5041 GGAGATCTGG AACGATAAAA CGATCTGGTA AAGTCAAAAA GAGAACACTC AATGGGAGTG
     5101 TAGAACTCCT TATTCCTCTT CAAGACAAAA TTCGTCAATT TATTTTTGAC AAGAAAATAG
     5161 CTATCCAAAA GAAAGATAGC TCATGGTTTC CAGTTCACAG GAAATATCTT ATTCGTTCAA
     5221 CAGACTTAGA AATCATCACA ATTTATAATT CTGAACTCCG CGGGATTTGT AATTACTACG
     5281 GTCTAGCAAG TAATTTTAAC CAGCTCAATT ATTTTGCTTA TCTTATGGAA TACAGCTGTC
     5341 TAAAAACGAT AGCCTCCAAA CATAAGGGAA CACTTTCAAA AACCATTTCC ATGTTTAAAG
     5401 ATGGAAGTGG TTCGTGGGGG ATCCCGTATG AGATAAAGCA AGGTAAGCAG CGCCGTTATT
     5461 TTGCAAATTT TAGTGAATGT AAATCCCCTT ATCAATTTAC GGATGAGATA AGTCAAGCTC
     5521 CTGTATTGTA TGGCTATGCC CGGAATACTC TTGAAAACAG GTTAAAAGCT AAATGTTGTG
     5581 AATTATGTGG GACGTCTGAT GAAAATACTT CCTATGAAAT TCACCATGTC AATAAGGTCA
     5641 AAAATCTTAA AGGCAAAGAA AAATGGGAAA TGGCAATGAT AGCGAAACAA CGTAAAACTC
     5701 TTGTTGTATG CTTTCATTGT CATCGTCACG TGATTCATAA ACACAAGTGA ATGTCGAGCA
     5761 CCCGTTCTCG GAGCACTGTC CGACCGCTTT GGCCGCCGCC CAGTCCTGCT CGCTTCGCTA
     5821 CTTGGAGCCA CTATCGACTA CGCGATCATG GCGACCACAC CCGTCCTGTG GATCGCCAAG
     5881 CTCGCCGATG GTAGTGTGGG GTCTCCCCAT GCGAGAGTAG GGAACTGCCA GGCATCAAAT
     5941 AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT ATCTGTTGTT TGTCGGTGAA
     6001 CGCTCTCCTG AGTAGGACAA ATCCGCCGGG AGCGGATTTG AACGTTGCGA AGCAACGGCC
     6061 CGGAGGGTGG CGGGCAGGAC GCCCGCCATA AACTGCCAGG CATCAAATTA AGCAGAAGGC
     6121 CATCCTGACG GATGGCCTTT TTGCGTTTCT ACAAACTCTT CCTGTCGTCA TATCTACAAG
     6181 CCATCCCCCC ACAGATACGG TAAACTAGCC TCGTTTTTGC ATCAGGAAAG CAGAACGCCA
     6241 TGAGCGGCCT CATTTCTTAT TCTGAGTTAC AACAGTCCGC ACCGCTGTCC GGTAGCTCCT
     6301 TCCGGTGGGC GCGGGGCATG ACTATCGTCG CCGCACTTAT GACTGTCTTC TTTATCATGC
     6361 AACTCGTAGG ACAGGTGCCG GCAGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA
     6421 TACCCACGCC GAAACAAGCG CCCTGCACCA TTATGTTCCG GATCTGCATC GCAGGATGCT
     6481 GCTGGCTACC CTGTGGAACA CCTACATCTG TATTAACGAA GCGCTAACCG TTTTTATCAG
     6541 GCTCTGGGAG GCAGAATAAA TGATCATATC GTCAATTATT ACCTCCACGG GGAGAGCCTG
     6601 AGCAAACTGG CCTCAGGCAT TTGAGAAGCA CACGGTCACA CTGCTTCCGG TAGTCAATAA
     6661 ACCGGTAAAC CAGCAATAGA CATAAGCGGC TATTTAACGA CCCTGCCCTG AACCGACGAC
     6721 CGGGTCGAAT TTGCTTTCGA ATTTCTGCCA TTCATCCGCT TATTATCACT TATTCAGGCG
     6781 TAGCACCAGG CGTTTAAGGG CACCAATAAC TGCCTTAAAA AAATTACGCC CCGCCCTGCC
     6841 ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC GACATGGAAG CCATCACAGA
     6901 CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT GTCGCCTTGC GTATAATATT
     6961 TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT GGCCACGTTT AAATCAAAAC
     7021 TGGTGAAACT CACCCAGGGA TTGGCTGAGA CGAAAAACAT ATTCTCAATA AACCCTTTAG
     7081 GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG CGAATATATG TGTAGAAACT
     7141 GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA CGTTTCAGTT TGCTCATGGA
     7201 AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG CTCACCGTCT TTCATTGCCA
     7261 TACG
//

