LOCUS pLenti\PGK\Hygro\pip-Ven-P2A-mCherry-Gem110 10661 bp DNA circular 5-JUN-2018 DEFINITION attB1-attB2. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|-12320145| COMMENT VNTDBDATE|-12320145| COMMENT LSOWNER| COMMENT VNTNAME|pLenti PGK Hygro pip-Ven-P2A-mCherry-Gem110| COMMENT VNTAUTHORNAME|Gavin Grant| COMMENT VNTAUTHORTEL|919-843-3935| COMMENT VNTAUTHOREML|gavin_grant@med.unc.edu| COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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misc_feature 3255..3398 /vntifkey="21" /label=lacZ_a misc_feature complement(3255..3277) /vntifkey="21" /label=M13_pUC_fwd_primer promoter complement(3246..3262) /vntifkey="30" /label=M13_forward20_primer promoter complement(3218..3236) /vntifkey="30" /label=T7_promoter misc_feature 3112..3131 /vntifkey="21" /label=SV40pro_F_primer rep_origin 3050..3127 /vntifkey="33" /label=SV40_origin misc_feature 3001..3020 /vntifkey="21" /label=EBV_rev_primer terminator 2913..3032 /vntifkey="43" /label=SV40_PA_terminator misc_feature 2658..2838 /vntifkey="21" /label=truncHIV-1_3_LTR misc_feature 2658..2838 /vntifkey="21" /label=HIV-1_5_LTR misc_feature 2588..2609 /vntifkey="21" /label=U3PPT misc_feature 2588..2603 /vntifkey="21" /label=cPPT gene 1221..2228 /gene="hygroB" /vntifkey="60" CDS complement(1134..1964) /vntifkey="4" /label=ORF\frame\2 misc_feature 1121..1140 /vntifkey="21" /label=mPGK_F_primer CDS 969..2231 /vntifkey="4" /label=ORF\frame\1 misc_feature complement(724..747) /vntifkey="21" /label=MSCV_rev_primer CDS 135..677 /vntifkey="4" /label=ORF\frame\1 misc_feature 47..622 /vntifkey="21" /label=WPRE misc_feature 7887..7902 /vntifkey="21" /label=cPPT misc_feature 7154..7387 /vntifkey="21" /label=RRE CDS 7032..8240 /vntifkey="4" /label=ORF\frame\3 misc_feature 6600..6644 /vntifkey="21" /label=HIV-1_psi_pack misc_feature 6312..6492 /vntifkey="21" /label=HIV-1_5_LTR misc_feature 6312..6492 /vntifkey="21" /label=truncHIV-1_3_LTR promoter 6038..6057 /vntifkey="30" /label=T3_promoter promoter 6003..6021 /vntifkey="30" /label=M13_reverse_primer misc_feature 5982..6004 /vntifkey="21" /label=M13_pUC_rev_primer promoter 5939..5968 /vntifkey="30" /label=lac_promoter rep_origin 5011..5630 /vntifkey="33" /label=pBR322_origin CDS 3996..4856 /vntifkey="4" /label=AmpR gene 3996..4856 /gene="Ampicillin" /vntifkey="60" promoter 3926..3954 /vntifkey="30" /label=AmpR_promoter primer_bind 10278..10298 /vntifkey="28" /label=R_mch_primer\binding\site primer_bind 10305..10327 /vntifkey="28" /label=F_gem_primer\binding\site primer_bind 10619..10637 /vntifkey="28" /label=R_Gem\primer\site primer_bind 9485..9540 /vntifkey="28" /label=F_Ven-Xho_P2A-gib misc_feature 9591..9609 /vntifkey="21" /label=F\_FP\primer\binding\site primer 9566..9583 /vntifkey="27" /label=P2A\R\primer\binding\site primer 9519..9540 /vntifkey="27" /label=P2A\F\primer\site misc_feature 9591..10298 /vntifkey="21" /label=mCherry misc_feature 10305..10634 /vntifkey="21" /label=Gem110 misc_feature 9519..9584 /vntifkey="21" /label=P2A misc_feature 8643..8735 /vntifkey="21" /label=Cdt1pip_NLS misc_feature 8799..9512 /vntifkey="21" /label=mVenus misc_feature 8994..9016 /vntifkey="21" /label=best\place\to\make\Ven\specific\primers CDS 8736..8792 /vntifkey="4" /label=HA2 BASE COUNT 2578 a 2797 c 2904 g 2382 t ORIGIN 1 ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgacaatcaa cctctggatt 61 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 121 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 181 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 241 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 301 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 361 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 421 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct 481 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 541 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga 601 cgagtcggat ctccctttgg gccgcctccc cgcctggaat tctgcagata tccggactag 661 tgatctctcg aggttaacga attctaccgg gtaggggagg cgcttttccc aaggcagtct 721 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct 781 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc 841 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg 901 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc 961 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc 1021 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc 1081 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag 1141 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcatcccg 1201 ccaccatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt 1261 tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct 1321 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca 1381 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg 1441 acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca 1501 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca 1561 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc 1621 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg 1681 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg 1741 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt 1801 tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg 1861 aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt 1921 tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat 1981 cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg 2041 ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat 2101 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg 2161 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg 2221 caaaggaata gagtagatgc cgaccgaaca agagctgatt tcgagaacgc ctcagccagc 2281 aactcgcgcg agcctagcaa ggcaaatgcg agagaacggc cttacgcttg gtggcacagt 2341 tctcgtccac agttcgctaa gctcgctcgg ctgggtcgcg ggagggccgg tcgcagtgat 2401 tcaggccctt ctggattgtg ttggtcccca gggcacgatt gtcatgccca cgcactcggg 2461 tgatctgact gatcccgcag attggagatc gccgcccgtg cctgccgatt gggtgcagat 2521 ccgtcgaggg cccggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 2581 actttttaaa agaaaagggg ggactggaag ggctagctca ctcccaacga agacaagatc 2641 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 2701 gctgcctagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 2761 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 2821 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg 2881 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca 2941 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 3001 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct 3061 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 3121 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 3181 aggcctaggc ttttgcgtcg agacgtaccc aattcgccct atagtgagtc gtattacgcg 3241 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 3301 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc 3361 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcgacgc gccctgtagc 3421 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc 3481 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt 3541 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac 3601 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag 3661 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa 3721 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg 3781 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac 3841 aaaatattaa cgtttacaat ttcccaggtg gcacttttcg gggaaatgtg cgcggaaccc 3901 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 3961 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 4021 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 4081 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 4141 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 4201 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 4261 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 4321 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 4381 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 4441 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 4501 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 4561 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 4621 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 4681 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 4741 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 4801 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 4861 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 4921 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 4981 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 5041 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 5101 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 5161 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 5221 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 5281 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 5341 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 5401 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 5461 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 5521 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 5581 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 5641 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 5701 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 5761 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc 5821 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 5881 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 5941 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 6001 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac 6061 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat 6121 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt 6181 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg 6241 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg 6301 atacaataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac 6361 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg 6421 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga 6481 aaatctctag cagtggcgcc cgaacaggga cctgaaagcg aaagggaaac cagagctctc 6541 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt 6601 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt 6661 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg 6721 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc 6781 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca 6841 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct 6901 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga 6961 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg 7021 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa 7081 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa 7141 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg 7201 gcgcagcctc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc 7261 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct 7321 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac 7381 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga 7441 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt 7501 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa 7561 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga 7621 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag 7681 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg 7741 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg 7801 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg 7861 gatctcgacg gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag 7921 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa 7981 ttacaaaaat tcaaaatttt atcgatcacg agactagcct cgagaagctt gatatcgaat 8041 tcccacgggg ttggggttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg 8101 ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt 8161 cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac 8221 gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg 8281 acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat 8341 ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcgg ggcgcgccga 8401 gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct 8461 gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg 8521 ctccctcgtt gaccgaatca ccgacctctc tccccagggg gatcaccggt cgtcgactag 8581 tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca ggcttcgaat 8641 tcatggagca gcgccgcgtc accgacttct tcgcgcgccg ccgccccggg ccccggacac 8701 gtggcccaaa aaagaagaga aaggtaggag ctcaataccc atacgatgtt cctgactatg 8761 cgggctatcc ctatgacgtc ccggactatg caactagtat ggtgagcaag ggcgaggagc 8821 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 8881 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga 8941 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg 9001 gcctgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg 9061 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca 9121 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 9181 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca 9241 gccacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga 9301 tccgccacaa catcgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc 9361 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccgccc 9421 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg 9481 ccgggatcac tctcggcatg gacgagctgt acctcgaggg aagcggagct actaacttca 9541 gcctgctgaa gcaggctgga gacgtggagg agaaccctgg acctcaattg atggtgagca 9601 agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg 9661 agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg 9721 agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg 9781 acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca 9841 tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact 9901 tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca 9961 tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga 10021 agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg 10081 gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga 10141 ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt 10201 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg 10261 gccgccactc caccggcggc atggacgagc tgtacaaggt cgagatgaat cccagtatga 10321 agcagaaaca agaagaaatc aaagagaata taaagaatag ttctgtccca agaagaactc 10381 tgaagatgat tcagccttct gcatctggat ctcttgttgg aagagaaaat gagctgtccg 10441 caggcttgtc caaaaggaaa catcggaatg accacttaac atctacaact tccagccctg 10501 gggttattgt cccagaatct agtgaaaata aaaatcttgg aggagtcacc caggagtcat 10561 ttgatcttat gattaaagaa aatccatcct ctcagtattg gaaggaagtg gcagaaaaac 10621 ggagaaaggc gctgtaacaa ttgggatcct acccagcttt c //