LOCUS       pLenti\CMV\Blast\-PIP-FUCCI        9692 bp    DNA     circular      1-AUG-2019
DEFINITION  attB1-attB2.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-12927524|
COMMENT     VNTDBDATE|-12927524|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pLenti CMV Blast -PIP-FUCCI|
COMMENT     VNTAUTHORNAME|Gavin Grant|
COMMENT     VNTAUTHORTEL|919-843-3935|
COMMENT     VNTAUTHOREML|gavin_grant@med.unc.edu|
FEATURES             Location/Qualifiers
     misc_feature    7642..7662
                     /vntifkey="21"
                     /label=attB1
     misc_feature    complement(9685..13)
                     /vntifkey="21"
                     /label=attB2
     rep_origin      2437..2743
                     /vntifkey="33"
                     /label=f1_origin
     misc_feature    2268..2411
                     /vntifkey="21"
                     /label=lacZ_a
     misc_feature    complement(2268..2290)
                     /vntifkey="21"
                     /label=M13_pUC_fwd_primer
     promoter        complement(2259..2275)
                     /vntifkey="30"
                     /label=M13_forward20_primer
     promoter        complement(2231..2249)
                     /vntifkey="30"
                     /label=T7_promoter
     misc_feature    2125..2144
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     rep_origin      2063..2140
                     /vntifkey="33"
                     /label=SV40_origin
     misc_feature    2014..2033
                     /vntifkey="21"
                     /label=EBV_rev_primer
     terminator      1926..2045
                     /vntifkey="43"
                     /label=SV40_PA_terminator
     misc_feature    1671..1851
                     /vntifkey="21"
                     /label=truncHIV-1_3_LTR
     misc_feature    1671..1851
                     /vntifkey="21"
                     /label=HIV-1_5_LTR
     misc_feature    1601..1622
                     /vntifkey="21"
                     /label=U3PPT
     misc_feature    1601..1616
                     /vntifkey="21"
                     /label=cPPT
     gene            1133..1531
                     /gene="blast"
                     /vntifkey="60"
     promoter        1065..1132
                     /vntifkey="30"
                     /label=EM7_promoter
     misc_feature    932..951
                     /vntifkey="21"
                     /label=SV40pro_F_primer
     rep_origin      870..947
                     /vntifkey="33"
                     /label=SV40_origin
     promoter        702..971
                     /vntifkey="30"
                     /label=SV40_promoter
     misc_feature    complement(690..906)
                     /vntifkey="21"
                     /label=SV40_enhancer
     misc_feature    complement(684..704)
                     /vntifkey="21"
                     /label=pBABE_3_primer
     CDS             455..967
                     /vntifkey="4"
                     /label=ORF\frame\3
     CDS             135..659
                     /vntifkey="4"
                     /label=ORF\frame\1
     CDS             complement(132..755)
                     /vntifkey="4"
                     /label=ORF\frame\2
     misc_feature    47..622
                     /vntifkey="21"
                     /label=WPRE
     misc_feature    7533..7557
                     /vntifkey="21"
                     /label=LNCX_primer
     misc_feature    7531..7550
                     /vntifkey="21"
                     /label=pCEP_fwd_primer
     promoter        7488..7557
                     /vntifkey="30"
                     /label=CMV_promoter
     misc_feature    7487..7507
                     /vntifkey="21"
                     /label=CMV_fwd_primer
     misc_feature    7033..7320
                     /vntifkey="21"
                     /label=CAG_enhancer
     promoter        7026..7530
                     /vntifkey="30"
                     /label=CMV_immearly_promoter
     misc_feature    6893..6908
                     /vntifkey="21"
                     /label=cPPT
     CDS             6506..7048
                     /vntifkey="4"
                     /label=ORF\frame\2
     misc_feature    6167..6400
                     /vntifkey="21"
                     /label=RRE
     CDS             6045..6545
                     /vntifkey="4"
                     /label=ORF\frame\3
     misc_feature    5613..5657
                     /vntifkey="21"
                     /label=HIV-1_psi_pack
     misc_feature    5325..5505
                     /vntifkey="21"
                     /label=HIV-1_5_LTR
     misc_feature    5325..5505
                     /vntifkey="21"
                     /label=truncHIV-1_3_LTR
     promoter        5051..5070
                     /vntifkey="30"
                     /label=T3_promoter
     promoter        5016..5034
                     /vntifkey="30"
                     /label=M13_reverse_primer
     misc_feature    4995..5017
                     /vntifkey="21"
                     /label=M13_pUC_rev_primer
     promoter        4952..4981
                     /vntifkey="30"
                     /label=lac_promoter
     rep_origin      4024..4643
                     /vntifkey="33"
                     /label=pBR322_origin
     CDS             3009..3869
                     /vntifkey="4"
                     /label=ORF\frame\3
     gene            3009..3869
                     /gene="Ampicillin"
                     /vntifkey="60"
     promoter        2939..2967
                     /vntifkey="30"
                     /label=AmpR_promoter
     primer_bind     9309..9329
                     /vntifkey="28"
                     /label=R_mch_primer\binding\site
     primer_bind     9336..9358
                     /vntifkey="28"
                     /label=F_gem_primer\binding\site
     primer_bind     9650..9668
                     /vntifkey="28"
                     /label=R_Gem\primer\site
     primer_bind     8516..8571
                     /vntifkey="28"
                     /label=F_Ven-Xho_P2A-gib
     misc_feature    8622..8640
                     /vntifkey="21"
                     /label=F\_FP\primer\binding\site
     primer          8597..8614
                     /vntifkey="27"
                     /label=P2A\R\primer\binding\site
     primer          8550..8571
                     /vntifkey="27"
                     /label=P2A\F\primer\site
     misc_feature    8622..9329
                     /vntifkey="21"
                     /label=mCherry
     misc_feature    9336..9665
                     /vntifkey="21"
                     /label=Gem110
     misc_feature    8550..8615
                     /vntifkey="21"
                     /label=P2A
     misc_feature    7674..7766
                     /vntifkey="21"
                     /label=Cdt1pip_NLS
     misc_feature    7830..8543
                     /vntifkey="21"
                     /label=mVenus
     misc_feature    8025..8047
                     /vntifkey="21"
                     /label=best\place\to\make\Ven\specific\primers
     CDS             7767..7823
                     /vntifkey="4"
                     /label=HA2
BASE COUNT     2500 a      2441 c      2497 g      2254 t 
ORIGIN
        1 ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgacaatcaa cctctggatt 
       61 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg 
      121 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct 
      181 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc 
      241 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca 
      301 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac 
      361 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt 
      421 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct 
      481 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc 
      541 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga 
      601 cgagtcggat ctccctttgg gccgcctccc cgcctggaat tctgcagata tccggttagt 
      661 aatgagtttg gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg 
      721 ctccccaggc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg 
      781 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 
      841 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc 
      901 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg 
      961 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa 
     1021 agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc 
     1081 atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa 
     1141 gcctttgtct caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat 
     1201 ccccatctct gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt 
     1261 cactggtgtc aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg 
     1321 cactgctgct gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa 
     1381 caggggcatc ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg 
     1441 gatcaaagcc atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt 
     1501 gctgccctct ggttatgtgt gggagggcta agcacaattc gagctcggta cctttaagac 
     1561 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg 
     1621 aagggctagc tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct 
     1681 ggttagacca gatctgagcc tgggagctct ctggctgcct agggaaccca ctgcttaagc 
     1741 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 
     1801 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt 
     1861 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga 
     1921 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 
     1981 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 
     2041 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc 
     2101 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 
     2161 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgcg tcgagacgta 
     2221 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt 
     2281 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 
     2341 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 
     2401 ctgaatggcg aatggcgcga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 
     2461 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 
     2521 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 
     2581 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt 
     2641 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 
     2701 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 
     2761 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag 
     2821 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttcccag 
     2881 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 
     2941 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 
     3001 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 
     3061 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 
     3121 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 
     3181 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 
     3241 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 
     3301 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 
     3361 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 
     3421 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 
     3481 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 
     3541 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 
     3601 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 
     3661 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 
     3721 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 
     3781 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 
     3841 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 
     3901 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 
     3961 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 
     4021 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 
     4081 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 
     4141 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 
     4201 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 
     4261 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 
     4321 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 
     4381 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 
     4441 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 
     4501 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 
     4561 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 
     4621 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 
     4681 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 
     4741 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 
     4801 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 
     4861 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 
     4921 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 
     4981 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg 
     5041 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta 
     5101 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt 
     5161 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc 
     5221 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca 
     5281 ttgcagagat attgtattta agtgcctagc tcgatacaat aaacgggtct ctctggttag 
     5341 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat 
     5401 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact 
     5461 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag 
     5521 ggacctgaaa gcgaaaggga aaccagagct ctctcgacgc aggactcggc ttgctgaagc 
     5581 gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg 
     5641 aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc 
     5701 gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat 
     5761 agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc 
     5821 agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga 
     5881 acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat 
     5941 aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac 
     6001 cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga 
     6061 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca 
     6121 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc 
     6181 ttgggttctt gggagcagca ggaagcacta tgggcgcagc ctcaatgacg ctgacggtac 
     6241 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg 
     6301 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa 
     6361 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg 
     6421 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg 
     6481 aacagattgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag 
     6541 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt 
     6601 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg 
     6661 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc 
     6721 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca 
     6781 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag 
     6841 agacagagac agatccattc gattagtgaa cggatctcga cggttaactt ttaaaagaaa 
     6901 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat 
     6961 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg 
     7021 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc 
     7081 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat 
     7141 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat 
     7201 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat 
     7261 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc 
     7321 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac 
     7381 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa 
     7441 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt 
     7501 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc 
     7561 tggagacgcc atccacgctg ttttgacctc catagaagac accgactcta gtccagtgtg 
     7621 gtggaattct gcagatatca acaagtttgt acaaaaaagc aggcttcgaa ttcatggagc 
     7681 agcgccgcgt caccgacttc ttcgcgcgcc gccgccccgg gccccggaca cgtggcccaa 
     7741 aaaagaagag aaaggtagga gctcaatacc catacgatgt tcctgactat gcgggctatc 
     7801 cctatgacgt cccggactat gcaactagta tggtgagcaa gggcgaggag ctgttcaccg 
     7861 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt 
     7921 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagctg atctgcacca 
     7981 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgggctac ggcctgcagt 
     8041 gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg 
     8101 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg 
     8161 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact 
     8221 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg 
     8281 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca 
     8341 acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg 
     8401 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccgcc ctgagcaaag 
     8461 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca 
     8521 ctctcggcat ggacgagctg tacctcgagg gaagcggagc tactaacttc agcctgctga 
     8581 agcaggctgg agacgtggag gagaaccctg gacctcaatt gatggtgagc aagggcgagg 
     8641 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg 
     8701 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc 
     8761 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt 
     8821 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact 
     8881 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg 
     8941 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg 
     9001 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg 
     9061 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca 
     9121 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca 
     9181 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca 
     9241 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact 
     9301 ccaccggcgg catggacgag ctgtacaagg tcgagatgaa tcccagtatg aagcagaaac 
     9361 aagaagaaat caaagagaat ataaagaata gttctgtccc aagaagaact ctgaagatga 
     9421 ttcagccttc tgcatctgga tctcttgttg gaagagaaaa tgagctgtcc gcaggcttgt 
     9481 ccaaaaggaa acatcggaat gaccacttaa catctacaac ttccagccct ggggttattg 
     9541 tcccagaatc tagtgaaaat aaaaatcttg gaggagtcac ccaggagtca tttgatctta 
     9601 tgattaaaga aaatccatcc tctcagtatt ggaaggaagt ggcagaaaaa cggagaaagg 
     9661 cgctgtaaca attgggatcc tacccagctt tc 
//
