LOCUS       Exported                4984 bp ds-DNA     circular SYN 16-JUN-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4984)
  AUTHORS   Leonard Lab
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jan 10, 2020 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4984
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    31..55
                     /label=TUPV1 Barcode
     promoter        91..115
                     /label=YB_TATA Minimal Promoter
                     /note="From Hansen et al., PNAS 2014"
     regulatory      145..154
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
     CDS             151..849
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    900..1124
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    1142..1166
                     /label=TUPV1 Barcode
     misc_feature    1190..1425
                     /label=cHS4
     misc_feature    1529..1764
                     /label=cHS4
     rep_origin      1893..2321
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      2391..2526
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    2660..2781
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2830..2846)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2854..2870
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2878..2908)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2923..2944
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3232..3817)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3988..4848)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4849..4953)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatcgaag acggtgccaa ggtcttgaca aacgagtgct agtacaagat
       61 ctgaattcga gaccacaggt ctcggatccc tctagagggt atataatggg ggccactagt
      121 ctactaccag agctcatcgc tagcgccacc atggtgagcg agctgattaa ggagaacatg
      181 cacatgaagc tgtatatgga gggcaccgtg aacaaccacc acttcaagtg tacctccgag
      241 ggcgaaggca agccctacga gggcacccag accatgagaa tcaaggcggt cgagggcggc
      301 cctctcccct tcgccttcga catcctggct accagcttca tgtacggcag caaaaccttc
      361 atcaaccaca cccagggcat ccccgacttc tttaagcagt ccttccccga gggcttcaca
      421 tgggagagag tcaccacata cgaagatggg ggcgtgctga ccgctaccca ggacaccagc
      481 ctccaggacg gctgcctcat ctacaacgtc aagatcagag gggtgaactt cccatccaac
      541 ggccctgtga tgcagaagaa aacactcggc tgggaggcct ccaccgaaac cctgtacccc
      601 gctgacggcg gcctggaagg cagagccgac atggccctga agctcgtggg cgggggccac
      661 ctgatctgca acttgaaaac cacatacaga tccaagaaac ccgctaagaa cctcaagatg
      721 cccggcgtct actatgtgga caggagactg gaaagaatca aggaggccga caaagaaacc
      781 tacgtcgagc agcacgaggt ggctgtggcc agatactgcg acctccctag caaactgggg
      841 cacagatgag cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac
      901 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
      961 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     1021 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     1081 ggaagtcaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcaggtcg
     1141 agggtacatg cgccttactc cttgtgagcg catcgcgata agctctagag ggacagcccc
     1201 cccccaaagc ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc
     1261 gcccggggct ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg
     1321 gacagcccgg gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc
     1381 tctttgagcc tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga
     1441 tttagaatga cagaatcata gaacggcctg cgtacggatg gggctctagc tctagagctt
     1501 gatatcgaat tcctgcagcc ccgctagagg gacagccccc ccccaaagcc cccagggatg
     1561 taattacgtc cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc
     1621 cggcgctccc cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag
     1681 gtggcacggg atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct
     1741 ggggggatac ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag
     1801 aacggcctgg gcgcgcctgg tggcgaacga tcaacagacg ggagagggca aaagtcttcg
     1861 ctgacagcga ccggactcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     1921 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     1981 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     2041 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     2101 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     2161 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     2221 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     2281 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     2341 cagttaggct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     2401 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     2461 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     2521 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggaggttggg
     2581 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     2641 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa
     2701 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     2761 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc
     2821 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     2881 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     2941 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     3001 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     3061 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     3121 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     3181 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     3241 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     3301 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     3361 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     3421 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     3481 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     3541 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     3601 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     3661 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     3721 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg
     3781 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3841 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3901 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3961 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     4021 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     4081 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag atccacgctc
     4141 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     4201 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     4261 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     4321 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     4381 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     4441 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     4501 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     4561 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     4621 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     4681 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     4741 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     4801 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     4861 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     4921 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     4981 cgtc
//
